Long non-coding RNA DIO3OS binds to microRNA-130b to restore radiosensitivity in esophageal squamous cell carcinoma by upregulating PAX9
Abstract Background: Esophageal squamous cell carcinoma (ESCC) ranks as one of the most fatal cancers worldwide for its aggression and unsatisfactory survival rate. The long non-coding RNA (lncRNA)-microRNA (miRNA)-mRNA axis has been highlighted as a potency biomarker for enhancing the radiosensitivity of ESCC. Hence, we investigated the functional mechanism of the DIO3OS/miR-130b/paired box 9 (PAX9) axis in the radioresistance of ESCC cells. Methods: In cell experiments, we altered the miR-130b expression in ESCC cells using mimics or inhibitors to examine its effects on ESCC cell activities in response to 4 Gy irradiation, as well as the involvement of DIO3OS and PAX9. Tumor xenograft experiments were then conducted to observe the effect of miR-130b, DIO3OS and PAX9 on radiosensitivity of ESCC ells in vivo . Results: miR-130b was found to be highly-expressed in the ESCC. Downregulated miR-130b inhibited proliferation, invasion and resistance to apoptosis in ESCC cells. DIO3OS and PAX9 were reduced in ESCC. A notable finding revealed that miR-130b could bind to DIO3OS and PAX9 respectively. DIO3OS could upregulate PAX9 by binding to miR-130b, which ultimately promoted the radiosensitivity of ESCC in vitro and in vivo . Conclusion: Taken together, DIO3OS upregulates the expression of PAX9 by binding to miR-130b, ultimately promoting the radiosensitivity of ESCC. Keywords: DIO3OS. MicroRNA-130b. Paired box 9. Radiosensitivity. Esophageal squamous cell carcinoma.