scholarly journals Study on the germination of nano - TiO 2   synthetic  seeds and the physiology of Dendrobium  officinale regenerated plants

2020 ◽  
Author(s):  
Jiemin Guan ◽  
Xiaoping Lai ◽  
Guifang Zhang ◽  
Shijie Li ◽  
Jiexian Guan ◽  
...  
Keyword(s):  
Growth And Development ◽  
Life Science ◽  
Reductase Activity ◽  
Synthetic Seeds ◽  
Dendrobium Officinale ◽  
Synthetic Seed ◽  
Nano Tio2 ◽  
Biological Technology ◽  
Regenerated Plants ◽  
Promote Plant Growth

Abstract Synthetic seed of biological technology has been suggested as an effective strategy to improve breeding. Nano-TiO2 is a photosemiconductor material widely used in life science and agriculture. Nano-TiO2 has been recently shown to improve photosynthetic function, promote plant growth and development，but the physiology mechanism is still unclear. The aim of the current study was to confirm whether or not nano-TiO2 synthetic seed has an effect on germination and physiology of Dendrobium officinale. Using D. officinale protocorm models, we demonstrate that appropriate nano-TiO2 leads to growth and development of germination seedlings, and improves the root activity, nitrate reductase activity and antioxidant system activity. Our data also provide a basis for the improvement of the preparation process and practical production of D. officinale synthetic seeds.

Effects of kitchen waste biofertilizer on growth and development of Wheat (Triticum aestivum L.) crop

2016 ◽  
Vol 2 (1-2) ◽  
Author(s):  
Shakti K. Prabhuji ◽  
Richa . ◽  
Shiraz A. Wajih ◽  
Rajesh K. Tiwari ◽  
Gaurav K. Srivastava
Keyword(s):  
Triticum Aestivum ◽  
Growth And Development ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Triticum Aestivum L ◽  
Kitchen Waste ◽  
Chemical Fertilizers ◽  
Chlorophyll Contents ◽  
Shoot Ratio ◽  
Root Shoot Ratio

The growth and development of Wheat (Triticum aestivum Linn.) plant has been studied using self-prepared kitchen waste biofertilizer. The experimental plants which were grown using the biofertilizer prepared using the kitchen biodegradable wastes excelled on all the parameters tested (rate of seed germination, root – shoot ratio, chlorophyll contents in leaves and Nitrate Reductase activity) in comparison to traditional chemical fertilizers and therefore, the prepared biofertilizer has been found to be more effective nutrient supplier than the traditional chemical fertilizers.

scholarly journals Synthetic Seed Production as a Tool for the Conservation and Domestication of Celastrus paniculatus: A Rare Medicinal Plant

2019 ◽  
pp. 1-8
Author(s):  
D. L. C. K. Fonseka ◽  
W. W. U. I. Wickramaarachchi ◽  
R. P. S. Madushani
Keyword(s):  
Sri Lanka ◽  
Medicinal Plant ◽  
Plant Species ◽  
Synthetic Seeds ◽  
Shoot Tips ◽  
Medicinal Plant Species ◽  
Synthetic Seed ◽  
Black Oil ◽  
Celastrus Paniculatus

The black-oil tree (Celastrus paniculatus Willd) is a highly valued medicinal plant species belong to the Celastraceae family, known as Jyothishmathi in Ayurveda and Duhundu in Sri Lanka and grows as a perennial vine. It is an endangered medicinal plant species recorded in the red list of endangered fauna and flora of Sri Lanka in 1999. The seed oil of Celastrus paniculatus contains sesquiterpene alkaloids namely; celapagine, celapanigine, celapanine and celastrol, used in traditional system of medicine for various disorders and because of its high pharmaceutical value, plants are over exploited in natural habitats. Owing to poor seed germination and lack of successful vegetative propagation methods, domestication and commercial planting of this important medicinal plant species to meet the demand seems impossible. Therefore, it is of high importance to develop a reliable and efficient in vitro propagation to produce black oil plants for commercial use. In this study, it was attempted to produce synthetic seeds of Celestrus paniculatus via in vitro multiple shoot proliferation. Nodal segment explants were collected from freshly emerged age of sprouts, surface sterilized and cultured in Murashige and Skoog medium supplemented with different 6-benzylaminopurine (BAP) and Thidiazuron (TDZ) concentrations for shoot induction. The highest soot proliferation rate; 25 shoot tips/explant were observed with 0.1 mg/L TDZ. Induced shoot tips were used for synthetic seed production after encapsulating with BAP and a-naphthalene acetic (NAA) enriched sodium alginate. Shoot tip encapsulated beads produced with 4% sodium alginate were firm, clear, round and uniform in size and easy to handle. The influence of growth regulators (BAP and NAA) and storage period on the germination of encapsulated shoot tips was studied to evaluate the success of encapsulated shoot tips as a propagule. The beads germinated with 2 mg/L BAP and 0.2 mg/L NAA provided 80% in vitro germination percentage. Shoot tips of synthetic seeds remained green and healthy after storage at 5°C for a period of 8 weeks. Current findings suggest that encapsulated micro shoots (synthetic seeds) could be produced successfully, as the first step in domestication and conservation of Celastrus paniculatus. Further studies required on rooting of micro shoots, acclimatization and transferring of plantlets produced from synthetic seeds to in vivo conditions for domestication and conservation purposes.

scholarly journals Introduction Pages

2019 ◽  
Vol 11 (2) ◽  
pp. I-VIII
Author(s):  
Radu E. SESTRAS
Keyword(s):  
Life Science ◽  
Biological Activities ◽  
Wildlife Sanctuary ◽  
Biotic And Abiotic Stresses ◽  
Regenerated Plants ◽  
Sloth Bear ◽  
Open Markets ◽  
Securinega Virosa ◽  
Cymbopogon Schoenanthus

Notulae Scientia Biologicae (http://www.notulaebiologicae.ro), Issue 2, Volume 11, 2019: The papers published in this issue (http://www.notulaebiologicae.ro/index.php/nsb/issue/current) represent interesting novelties in different topics of life science. Among the exciting researches, we invite readers to find news about: endophytic bacteria - hidden protective associates of plants against biotic and abiotic stresses (review); Occurrence of Salmonella in raw chicken meat from retail equipment and environments in southern Nigeria open markets; fruiting species influence the seasonal use of the habitat by sloth bear in and around Balaram Ambaji Wildlife Sanctuary, Gujarat, India; age susceptibility of wistar rats to alloxan-induced diabetes: a paradox; anti-ulcerogenic potential of aqueous extract of Securinega virosa leaf in indomethacin-induced ulcerated rats; evaluation of various biological activities of endemic Sideritis libanotica extracts; genetic polymorphism of the wild and in vitro regenerated plants of the medicinal grass Cymbopogon schoenanthus subsp. Proximus etc.

scholarly journals Synthetic Seed Technology Development and Production Studies for Storage, Transport, and Industrialization of Bracken Spores

Plants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1079
Author(s):  
Bo Kook Jang ◽  
Ju Sung Cho ◽  
Cheol Hee Lee
Keyword(s):  
Technology Development ◽  
Synthetic Seeds ◽  
Pteridium Aquilinum ◽  
Spore Density ◽  
Synthetic Seed ◽  
Seed Technology ◽  
Production Studies ◽  
Long Term Storage ◽  
Propagation Methods

Bracken fern (Pteridium aquilinum var. latiusculum (Desv.) Underw. ex A. Heller) has long been grown industrially in South Korea. Conventional propagation methods, including planting rhizomes and in vitro seedling culture, are labor intensive and expensive, and thus not commercially suitable. We aimed to develop a system to produce synthetic seeds using fern spores (SFS). Synthetic seeds were prepared by mixing bracken spores and alginate matrix. Spore germination and gametophyte and sporophyte growth and development from SFS proceeded normally. Spore density affected gametophyte and sporophyte numbers. SFS prepared using cold (4 °C) long-term storage spores (even 7-year-old spores) could effectively form sporophytes. The highest germination was observed at 25 °C. Soaking-treated SFS successfully formed sporophytes, even after 30 days of storage at 4 °C; indeed, sporophytes formed even after five days of storage at 25 °C during transport conditions. SFS were sown in plug trays for commercial use. Young sporophytes grown from plug seedlings were greenhouse cultivated, and transplanting within eight weeks was effective for root growth and growing-point formation. Developing synthetic seeds is a feasible solution for facilitating efficient transport and the handling of small-sized fern spores; furthermore, this SFS technology provides the basis for fern seedling culture and fern spore industrialization.

The GDP-mannose transporter gene (DoGMT) from Dendrobium officinale is critical for mannan biosynthesis in plant growth and development

Plant Science ◽  
2018 ◽  
Vol 277 ◽  
pp. 43-54 ◽  
Author(s):  
Zhenming Yu ◽  
Chunmei He ◽  
Jaime A. Teixeira da Silva ◽  
Jianping Luo ◽  
Ziyin Yang ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth And Development ◽  
Dendrobium Officinale ◽  
Transporter Gene ◽  
Plant Growth And Development

scholarly journals Pengaruh matriks kapsul terhadap perkecambahan benih sintetik teh (Camellia sinensis L.) Effect of capsule matrix on germination of synthetic seeds of tea (Camellia sinensis L.)

2016 ◽  
Vol 83 (2) ◽  
Author(s):  
. SUMARYONO ◽  
Rizka T SAPTARI
Keyword(s):  
Sodium Alginate ◽  
Camellia Sinensis ◽  
Somatic Embryos ◽  
Germination Rate ◽  
Clonal Plant ◽  
Physical Characteristics ◽  
Synthetic Seeds ◽  
Synthetic Seed ◽  
Plant Materials ◽  
Seed Technology

Abstract         Synthetic seed technology has been developed to combine the practical use of ordinary seeds and clonal plant materials. Single somatic embryos of tea (Camellia sinensis L.) clone GMB 9 were encap-sulated using sodium alginate and CaCl2 at different concentrations to produce synthetic seeds.  Encapsulating matrices with and without somatic embryos were made of WP medium and 20 g/L sucrose with   1, 2 or 3%  sodium alginate and 50 or 100 mM CaCl2. Encapsulating matrices without somatic embryos were then tested its physical characteristics, whereas the capsules with somatic embryos were observed its germination rate and secondary embryo formation every week up to six weeks. The results showed that the concentrations of sodium alginate and CaCl2 were affected significantly the physical characteristics of encap-sulating matrix produced. The sufficient level of hardness and highest germination rate was obtained from 2% sodium alginate and 50 mM CaCl2. Sodium alginate at 1% or less produced soft, leaky and oval encapsulating matrices which were not suitable for synthetic seeds. Sodium alginate 3% and 100 mM CaCl2  produced rounded and very hard encapsulating matrices and inhibited the germination of somatic embryos. Germination rates of tea synthetic seeds and somatic embryos without encapsulation were 5 to 20% after six weeks. Abstrak         Teknologi benih sintetik dikembangkan untuk memadukan kepraktisan penggunaan benih biasa dengan bahan tanam klonal. Embrio somatik tunggal dari tanaman teh (Camellia sinensis L.) klon GMB 9 dienkapsulasi menggunakan natrium alginat   dan CaCl2 untuk membuat benih sintetik. Matriks kapsul dengan dan tanpa embrio somatik dibuat dari medium WP dengan sukrosa 20 g/L dicampur dengan natrium alginat 1, 2, atau 3% dan CaCl2 50  atau 100 mM.  Matriks  kapsul  tanpa embrio somatik kemudian diuji sifat fisiknya, sedangkan matriks kapsul dengan embrio somatik diamati daya kecambah dan pembentukan embrio somatik sekunder setiap minggu sampai dengan enam minggu. Hasil penelitian menunjukkan bahwa konsentrasi natrium alginat dan CaCl2 sangat berpengaruh terhadap sifat fisik matriks kapsul yang dihasilkan. Tingkat kekerasan yang memadai dan menghasilkan persentase perkecambahan yang tinggi diperoleh dengan alginat 2% dan CaCl2 50 mM. Natrium alginat pada konsentrasi 1% atau kurang menghasilkan matriks kapsul yang  lunak, mudah bocor dan berbentuk lonjong sehingga tidak sesuai untuk benih sintetik. Natrium alginat konsentrasi 3% pada larutan CaCl2 100 mM menghasilkan benih sintetik yang bulat, sangat keras dan menghambat perkecambahan embrio somatik teh.  Daya kecambah benih sintetik dan embrio somatik teh tanpa enkapsulasi berkisar  5 - 20% setelah enam minggu. 

scholarly journals The effect of cytokinins in vitro on the growth and development of Elatior begonias (Begonia Elatior-hybr.)

1991 ◽  
Vol 63 (2) ◽  
pp. 137-142
Author(s):  
Päivi Roivainen
Keyword(s):  
Somaclonal Variation ◽  
Nutrient Medium ◽  
Growth And Development ◽  
Flowering Plants ◽  
Growth Substances ◽  
Regenerated Plants ◽  
Number Of Shoots

Micropropagation involves the risk of somaclonal variation among the regenerated plants. It is possible that the components of the nutrient medium, especially growth substances, increase the frequency of variation. Elatior Begonia 'Barbara' plants were micropropagatedusing four cytokinins (zeatin 0.5—2.0 mg/l, kinetin 0.5—2.0 mg/l, IPA 0.5—2.0 mg/l or BAP 0.05—0.65 mg/l) in vitro. The number of shoots produced per explant and the subsequent quality of full-sized, flowering plants were studied. In general, the number of big (> 1 cm) and medium-sized (0.5 —1.0 cm) shoots decreased and the number of small (

scholarly journals Callus Induction and Synthetic Seed Development in Draceana sanderiana Sanderex Mast: Lucky Bamboo

2019 ◽  
pp. 1-8
Author(s):  
Mohmad Amin ◽  
Abdul Mujeeb
Keyword(s):  
Sodium Alginate ◽  
Seed Development ◽  
Callus Induction ◽  
Callus Formation ◽  
Synthetic Seeds ◽  
Shoot Tips ◽  
Synthetic Seed ◽  
Ms Medium ◽  
Seed Formation ◽  
Light Yellow

The study was carried out for callus induction and synthetic seed development from the shoot tips of Draceana sanderiana sander ex Mast. The shoot tips were subjected to different concentrations (0.25, 0.5 &1.0 mg/l) of 2,4-D on MS medium. The research findings revealed that the 2,4-D at concentrations of 0.25 mg/l was more suited for the profuse callus formation. The friable and light yellow callus was induced within 2 weeks of culture at 0.25 mg/l of 2,4-D on MS medium as compared to the other two concentrations of 2,4-D i.e.; 0.5 and 1.0 mg/l. Similarly the effect of sodium alginate and calcium chloride percentage on synthetic seed formation was observed, it was found that somatic embryos formed from shoot tips via callus kept in 2.5% sodium alginate and 100 milli molar CaCl2 produced synthetic seeds with firm spherical beads. The study leads to the formation of synthetic seeds of Draceana sanderiana which can be used for the conservation of germplasm through cryopreservation and the micro propagation of the said plant species.

scholarly journals Preparation of Synthetic Seeds of Citrus jambhiri Using in vitro Regenerated Multiple Plantlets

2020 ◽  
pp. 22-29
Author(s):  
Priyanka Sharma ◽  
Bidhan Roy
Keyword(s):  
Germplasm Conservation ◽  
Synthetic Seeds ◽  
Shoot Tips ◽  
Nodal Segments ◽  
Synthetic Seed ◽  
Ms Medium ◽  
Meristematic Tissue ◽  
Citrus Jambhiri ◽  
Cacl2 Solution

Biotechnological tools are useful for true-to-type propagation. Shoot tips encapsulation is potential for plant development from pre-existing meristematic tissue. MS medium fortified with 1 and 2 mg/L of BAP (6-bezylaminopurine) was found to be suitable for in vitro mass-multiplication of plantlets (10.18 and 13.05 plantlets/explant, respectively) of Citrus jambhiri from nodal segments. Nodal segments were more appropriate than the shoot tips for in vitro multiplication of plantlets. Synthetic seeds were prepared using 2.5% sodium alginate dropping into 3.0% CaCl2 solution. Maximum germination was recorded when beaded shoot tips were cultured on MS medium fortified with 1 and 2 mg/L of BAP (96.67 and 100.00%, respectively). However, the germination of synthetic seeds was found to be comparatively high than the earlier findings. The results support the use of encapsulated unipolar explants for synthetic seed preparation. These type of capsules could be useful in exchange of sterile material between laboratories, germplasm conservation and direct plant propagation.

Sign in / Sign up

Export Citation Format

Share Document