scholarly journals An Elevated High-Density Lipoprotein Cholesterol Levels Is Associated with Favorable Outcomes of Patients with Small-Artery Occlusion

2019 ◽  
Author(s):  
Hao Ruixiao ◽  
Liu Peipei ◽  
Wang Yajing ◽  
Jialing Wu
Keyword(s):  
High Density Lipoprotein ◽  
High Density Lipoprotein Cholesterol ◽  
Primary Outcome ◽  
Density Lipoprotein ◽  
Hdl Cholesterol ◽  
Artery Occlusion ◽  
High Density ◽  
Lipoprotein Cholesterol ◽  
Small Artery ◽  
Small Artery Occlusion

Abstract Background: The relationship between high-density lipoprotein (HDL) cholesterol and small-artery occlusion (SAO) is not well understood. Methods: A total of 3067 consecutive patients with SAO were recruited from Tianjin Huanhu Hospital between January 01, 2008, and December 31, 2015. HDL values at admission were classified into four groups according to quartiles (<0.92, 0.92–1.08, 1.08–1.28, and ≥1.28). Patients were followed up to 12 months after stroke. Prognoses, represented by modified Rankin scale (mRS), were estimated via HDL quartiles upon admission utilizing multivariate logistic regression analysis. Meanwhile, we conducted additional subgroup analyses to investigate associations according to age. Results: Among 3067 patients, 2284(74.5%) were classified as having favorable outcomes, 783(25.5%) had a composite of poor outcomes,recurrent stroke, myocardial infarction or vascular death within 12 months. After adjustment for possible confounders, HDL levels in the two highest quartiles (1.08–1.28 and ≥1.28) were correlated with the 12-month primary outcome of patients with SAO (1.08–1.28 , 0.587; 95% CI,0.394-0.873; P = 0.009; ≥1.28, 0.448; 95% CI, 0.291-0.688; P < 0.001). However, a direct correlation was found in patients aged 45–75 years (1.08–1.28, P = 0.033; ≥1.28, P = 0.001).Similar to the primary outcome,a direct correlation was also found in patients aged 45–75 years with a 12-month secondary outcomes(≥1.28 mmol/L, p = 0.001). Conclusions: An elevated HDL cholesterol level in patients with SAO is an independent predictor of a favorable prognosis 12 months after SAO. However, the association was only present in patients aged 45–75 years. Keywords: High-Density Lipoprotein Cholesterol, Stroke, Outcome, Small-Artery Occlusion.

scholarly journals Evaluation of two homogeneous methods for measuring high-density lipoprotein cholesterol

1997 ◽  
Vol 43 (6) ◽  
pp. 1048-1055 ◽  
Author(s):  
Yi-Chang Huang ◽  
Jau-Tsuen Kao ◽  
Keh-Sung Tsai
Keyword(s):  
Polyethylene Glycol ◽  
High Density Lipoprotein ◽  
High Density Lipoprotein Cholesterol ◽  
Phosphotungstic Acid ◽  
Density Lipoprotein ◽  
Hdl Cholesterol ◽  
High Density ◽  
Lipoprotein Cholesterol ◽  
Homogeneous Assays ◽  
Better Than

Abstract We evaluated the performance of two homogeneous assays for quantifying HDL cholesterol (HDL-C) and compared them with the phosphotungstic acid (PTA)/MgCl2 assay. Both homogeneous HDL-C assays were precise, having a within-run CV of &lt;1.20% and a between-run CV of &lt;4.07%. The HDL-C values (y) measured by the two homogeneous methods correlated well with those by the PTA/MgCl2 method (x): y = 1.00x + 64.98 mg/L, r = 0.987, Sy|x = 27.99 mg/L (n = 152) for the polyethylene glycol-modified enzymes/α-cyclodextrin sulfate (PEGME) assay (Kyowa), and y = 0.84x + 106.51 mg/L, r = 0.984, Sy|x = 26.10 mg/L (n = 152) for the polyanion–polymer/detergent (PPD) assay (Daiichi). The specificity of the PEGME method seemed better than that of the PPD method, as the PPD method was markedly interfered with by supplemental LDL-C. Addition of 20 g/L triglycerides produced a negative error of ∼18% in both homogeneous assays. Bilirubin and hemoglobin had little influence on the PEGME method; hemoglobin had little effect on the PPD method. Bilirubin, however, markedly decreased the readings by the PPD method. We found the PEGME assay superior to the PPD assay for routine HDL-C testing, because the PPD assay is relatively inaccurate and not specific.

Effects of Smoking on Oral Fat Tolerance and High Density Lipoprotein Cholesterol

1983 ◽  
Vol 65 (6) ◽  
pp. 669-672 ◽  
Author(s):  
R. S. Elkeles ◽  
S. R. Khan ◽  
V. Chowdhury ◽  
M. B. Swallow
Keyword(s):  
High Density Lipoprotein ◽  
High Density Lipoprotein Cholesterol ◽  
Density Lipoprotein ◽  
Serum Triglyceride ◽  
Hdl Cholesterol ◽  
High Density ◽  
Lipoprotein Cholesterol ◽  
Fatty Meal ◽  
Cholesterol Ratio ◽  
Average Serum

1. Changes in serum triglyceride and high density lipoprotein (HDL) cholesterol after a fatty meal have been studied in smokers and non-smokers. 2. Average serum triglyceride during the study was higher in smokers than in non-smokers. 3. In non-smokers there was a rise in the HDL2/HDL3 cholesterol ratio after oral fat, but not in smokers. 4. These findings are compatible with the hypothesis that smoking interferes with the lipolysis of triglyceride rich lipoproteins and the conversion of HDL3 into HDL2.

High-density-lipoprotein cholesterol in heparin-MnCl2 supernates determined with the Dow enzymic method after precipitation of Mn2+ with HCO3-.

1984 ◽  
Vol 30 (6) ◽  
pp. 839-842 ◽  
Author(s):  
P S Bachorik ◽  
R E Walker ◽  
D G Virgil
Keyword(s):  
High Density Lipoprotein ◽  
Disease Control ◽  
High Density Lipoprotein Cholesterol ◽  
Density Lipoprotein ◽  
Hdl Cholesterol ◽  
Comparison Method ◽  
High Density ◽  
Lipoprotein Cholesterol ◽  
Plasma Samples ◽  
Fresh Plasma

Abstract Manganese interferes with enzymic cholesterol methods. In this study, we enzymically measured high-density-lipoprotein (HDL) cholesterol in heparin-Mn2+ supernates that had been treated with NaHCO3 (91 mmol/L) to precipitate Mn2+, and compared results with those by an automated Liebermann- Burchard method. For untreated supernates of 96 fresh plasma samples, the enzymic values were 10.4% higher than comparison-method values, a bias that declined to +2.3% for treated supernates. For 72 sera promptly frozen and stored after collection, the enzymic values for untreated and treated supernates were, respectively, 6.0% and 0.5% higher than comparison-method values. In all cases, the magnitude of the bias was independent of the concentrations of cholesterol, triglyceride, and HDL-cholesterol. Enzymic HDL-cholesterol measurements in NaHCO3-treated heparin-Mn2+ supernates prepared from four pooled serum controls agreed within 21 mg/L with values established for these pools by the Centers for Disease Control. We conclude that the accuracy of enzymic HDL cholesterol measurements in heparin-Mn2+ supernates in considerably increased by treatment with NaHCO3.

High density lipoprotein cholesterol and coronary artery occlusion

Metabolism ◽  
1979 ◽  
Vol 28 (7) ◽  
pp. 735-738 ◽  
Author(s):  
Joseph J. Barboriak ◽  
Alfred J. Anderson ◽  
Alfred A. Rimm ◽  
James F. King
Keyword(s):  
Coronary Artery ◽  
High Density Lipoprotein ◽  
High Density Lipoprotein Cholesterol ◽  
Density Lipoprotein ◽  
Coronary Artery Occlusion ◽  
Artery Occlusion ◽  
High Density ◽  
Lipoprotein Cholesterol

scholarly journals A Biochemical Study Of High-Density Lipoprotein Cholesterol (Hdl-C) Changes In Middle Aged Common People With Different Lifestyle

2017 ◽  
Vol 16 (2) ◽  
pp. 289-294
Author(s):  
Suresh Kanna ◽  
Premila Thamizhvanan ◽  
Jaya Bharathi
Keyword(s):  
Physical Activity ◽  
Body Weight ◽  
Young Adult ◽  
High Density Lipoprotein ◽  
High Density Lipoprotein Cholesterol ◽  
Density Lipoprotein ◽  
Hdl Cholesterol ◽  
Lifestyle Factors ◽  
High Density ◽  
Lipoprotein Cholesterol

Background and rationale: HDL cholesterol is one of the 5 major groups of lipoproteins cholesterol, which enable lipids like cholesterol and TG to be transported within the water based blood stream. In healthy persons, about thirty percent of blood cholesterol is carried by HDL cholesterol. HDL-C is a potent predictor of coronary heart disease. Genetic as well as environmental factors including lifestyle factors play a role as determinants of its level in the blood. To examine the effects of certain lifestyle factors on serum level of high density lipoprotein cholesterol in young adult people HDL cholesterol seems to protect against CVD which increases the risk for heart disease.Subjects and methods: Three hundred and twenty five young adult subjects of both sexes aged 18-45 years asymptomatic for cardiovascular diseases were interviewed according to special questionnaire including information on lifestyle habits. Physical examination was done, height, body weight, and blood pressure measurements were performed. Blood analysis to determine the blood level of high density lipoprotein cholesterol was done after 12 hours fasting.Results and conclusion: Smoking and obesity were the most significant risk factors associated with a decreased level of high density lipoprotein cholesterol. The level of HDL-C was 50.5±11.5 mg/dl in smokers compared with 57.7±12.5 mg/dl in nonsmokers. Its level was 48.5 ±8.5 mg/dl in obese individuals compared to 57.5±11.7mg/dl in normal body weight subjects. Physical activity was not significantly associated with low level of HDL-C analysis, but it was found to be significantly associated with its level by the multiple regression analysis. High-density lipoprotein cholesterol level was a function of many factors, some of them were lifestyle related such as smoking, physical activity, and obesity. Therefore, efforts to encourage more physical activity, quitting smoking, consuming low fat diet, and keeping ideal body weight are recommended.Bangladesh Journal of Medical Science Vol.16(2) 2017 p.289-294

Polyacrylamide gel electrophoresis in quantification of high-density lipoprotein cholesterol.

1985 ◽  
Vol 31 (11) ◽  
pp. 1893-1895 ◽  
Author(s):  
D Roche ◽  
V Atger ◽  
N T Le Quang ◽  
A Girard ◽  
O G Ekindjian
Keyword(s):  
High Density Lipoprotein ◽  
High Density Lipoprotein Cholesterol ◽  
Polyacrylamide Gel Electrophoresis ◽  
Density Lipoprotein ◽  
Hdl Cholesterol ◽  
Polyacrylamide Gel ◽  
High Density ◽  
Lipoprotein Cholesterol ◽  
Wide Range ◽  
Ultracentrifugal Analysis

Abstract We evaluated a method for quantifying high-density lipoprotein cholesterol in plasma, based on electrophoretic migration of the prestained (with Sudan Black III) sample through a discontinuous polyacrylamide++ gel and densitometric integration of the stain associated with each class of lipoprotein. With this method, operations can be carried out on all types of lipoproteins over a broad range of concentrations. Overloading with very-low and low-density lipoproteins did not affect reliability within a wide range of HDL concentrations (0.45 to 16.60 mmol/L). Results for 22 individual plasma samples from normal and dyslipemic subjects correlated well with those by ultracentrifugal analysis (r=0.96; Student's t= 0.90, p &gt; 0.30). We conclude that this method is reliable, sensitive, and accurate, It may be used for simultaneously typing dyslipoproteinemias and assaying HDL cholesterol.

Association between the cholesteryl ester transfer protein TaqI-detectable B polymorphism and low high-density lipoprotein cholesterol concentration in Saudis

2003 ◽  
Vol 105 (4) ◽  
pp. 467-472 ◽  
Author(s):  
Nassr M. AL-DAGHRI ◽  
Omer AL-ATTAS ◽  
Ashok PATEL ◽  
Nikolai D. BELYAEV ◽  
William A. BARTLETT ◽  
...  
Keyword(s):  
High Density Lipoprotein ◽  
Cholesteryl Ester ◽  
High Density Lipoprotein Cholesterol ◽  
Density Lipoprotein ◽  
Hdl Cholesterol ◽  
High Density ◽  
Lipoprotein Cholesterol ◽  
Cholesterol Concentration ◽  
Cholesterol Levels ◽  
Significant Difference

Plasma concentrations of HDL (high-density lipoprotein) cholesterol are low in the Saudi Arabian population. A B polymorphism at the CETP (cholesteryl ester protein transfer) locus that is detectable with the restriction enzyme TaqI is a genetic determinant of the plasma HDL cholesterol concentration. We assessed the relationship between the TaqI B CETP polymorphism and lipid and apolipoprotein concentrations in a study sample of 335 Saudi residents. The TaqI B1 and B2 allele frequencies were 0.54 and 0.46 respectively, similar to those in other populations. HDL cholesterol levels in B2B2 homozygotes were significantly higher than in B1B1 homozygotes [1.01 (0.3) compared with 0.92 (0.2) mmol/l; mean (S.D.); P=0.03]. There was also a significant difference between the B2B2 and B1B1 homozygotes with regard to apolipoprotein AI concentration [123.6 (16.4) compared with 113.7 (13.9) mg/dl; P=0.04]. This genetic variation was independent of metabolic risk factors known to influence HDL cholesterol levels. The allele frequency of the TaqI B CETP polymorphism and its relatively modest impact on HDL cholesterol concentrations argue against an important role for this allele, or for strongly linked loci, in determining the low levels of HDL cholesterol seen in the Saudi population.

Comparison of improved precipitation methods for quantification of high-density lipoprotein cholesterol.

1985 ◽  
Vol 31 (2) ◽  
pp. 217-222 ◽  
Author(s):  
G R Warnick ◽  
T Nguyen ◽  
A A Albers
Keyword(s):  
Polyethylene Glycol ◽  
High Density Lipoprotein ◽  
High Density Lipoprotein Cholesterol ◽  
Dextran Sulfate ◽  
Density Lipoprotein ◽  
Hdl Cholesterol ◽  
High Density ◽  
Protein Precipitation ◽  
Lipoprotein Cholesterol ◽  
Lipid Research

Abstract We compared the standard Lipid Research Clinics heparin-Mn2+ (46 mmol/L) method and five improved precipitation methods for quantification of high-density lipoprotein (HDL) cholesterol. Three of these methods--a dextran sulfate-Mg2+ procedure, reported as a Selected Method, a modified heparin-Mn2+ (92 mmol/L) method, and a modified phosphotungstate-Mg2+ procedure--all gave similar results. Three other methods--the standard heparin-Mn2+ (46 mmol/L) method and two polyethylene glycol methods (75 g/L or pH 10 reagent at 100 g/L final concentrations)--gave slightly higher values for HDL cholesterol. Addition of NaCl or glucose to specimens did not significantly change protein precipitation. In terms of sedimentation effectiveness with hypertriglyceridemic specimens, the methods were ranked in the following order: polyethylene glycol (pH 10, 100 g/L) greater than dextran sulfate-Mg2+ greater than heparin-Mn2+ (92 mmol/L) = polyethylene glycol (75 g/L) greater than phosphotungstate-Mg2+ greater than heparin-Mn2+ (46 mmol/L).

scholarly journals Inflammation Alters Relationship Between High‐Density Lipoprotein Cholesterol and Cardiovascular Risk in Patients With Chronic Kidney Disease: Results From KNOW‐CKD

2021 ◽  
Author(s):  
Jae Young Kim ◽  
Jung Tak Park ◽  
Hyung Woo Kim ◽  
Tae‐Ik Chang ◽  
Ea Wha Kang ◽  
...  
Keyword(s):  
Chronic Kidney Disease ◽  
Kidney Disease ◽  
High Density Lipoprotein ◽  
High Density Lipoprotein Cholesterol ◽  
Cardiovascular Events ◽  
Primary Outcome ◽  
Density Lipoprotein ◽  
High Density ◽  
Lipoprotein Cholesterol ◽  
Major Adverse Cardiovascular Events

Background The function of high‐density lipoprotein can change from protective to proatherosclerotic under inflammatory conditions. Herein, we studied whether inflammation could modify the relationship between high‐density lipoprotein level and risk of adverse outcomes in patients with chronic kidney disease . Methods and Results In total, 1864 patients from the prospective KNOW‐CKD (Korean Cohort Study for Outcome in Patients With Chronic Kidney Disease) were enrolled. The main predictor was high‐density lipoprotein cholesterol (HDL‐C) level. Presence of inflammation was defined by hs‐CRP (high‐sensitivity C‐reactive protein) level of ≥1.0 mg/L. The primary outcome was extended major adverse cardiovascular events. During 9231.2 person‐years of follow‐up, overall incidence of the primary outcome was 15.8 per 1000 person‐years. In multivariable Cox analysis after adjusting for confounders, HDL‐C level was not associated with the primary outcome. There was a significant interaction between the inflammatory status and HDL‐C for risk of extended major adverse cardiovascular events ( P =0.003). In patients without inflammation, the hazard ratios (HRs) (95% CIs) for HDL‐C levels <40, 50 to 59, and ≥60 mg/dL were 1.10 (0.50–1.82), 0.95 (0.50–1.82), and 0.42 (0.19–0.95), respectively, compared with HDL‐C of 40 to 49 mg/dL. However, the significant association for HDL‐C ≥60 mg/dL was not seen after Bonferroni correction. In patients with inflammation, we observed a trend toward increased risk of extended major adverse cardiovascular events in higher HDL‐C groups (HRs [95% CIs], 0.73 [0.37–1.43], 1.24 [0.59–2.61], and 1.56 [0.71–3.45], respectively), but without statistical significance. Conclusions The association between HDL‐C level and adverse cardiovascular outcomes showed reverse trends based on inflammation status in Korean patients with chronic kidney disease. Registration URL: https://www.clinicaltrials.gov ; Unique identifier: NCT01630486.

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