scholarly journals Physiological Changes in Chicken Embryos Inoculated with Drugs and Viruses Highlight the Need for More Standardisation of This Animal Model

2021 ◽  
Author(s):  
Simone Sommerfeld ◽  
Antonio Mundim ◽  
Rogério Silva ◽  
Jéssica Queiroz ◽  
Maisa Rios ◽  
...  
Keyword(s):  
Experimental Model ◽  
Viral Infections ◽  
Blood Parameters ◽  
Physiological Changes ◽  
Blood Cell Count ◽  
In Ovo ◽  
Chicken Embryos ◽  
Microscopic Damage ◽  
Toxicity Of Drugs ◽  
And Oxidative Stress

Abstract Several works have been developed using the Gallus gallus embryo as an experimental model to study the toxicity of drugs and infections. Studies that seek to standardise the evaluated parameters are needed to better understand and identify the viability of chicken embryos (CE) as an experimental model. Therefore, we sought to verify whether macroscopic, histopathological, blood count, metabolites and/or enzymes changes and oxidative stress in CE of different ages are peculiar to the model. To achieve this goal, in ovo assays were performed injecting a virus (Gammacoronavirus) and two drugs (filgrastim and dexamethasone) that cause known changes in adult chickens and other animals. Embryo response to virus and drug challenges may not occur as expected for adult chickens and even different species. While macroscopic and microscopic damage was visible in the case of viral infections, the white blood cell count and inflammation biomarkers did not change. Filgrastim (FG) testing did not result in the expected effects for CE. On the other hand, with dexamethasone (DX), changes in blood parameters and biomarkers were inherent to the model and depended on the stage of CE development. Our work reinforces the importance of standardization and correct use of the model so that the results of infection, toxicity and pharmacokinetics are recorded.

scholarly journals Physiological Changes in Chicken Embryos Inoculated With Drugs and Viruses Highlight the Need for More Standardisation of This Animal Model

2021 ◽  
Author(s):  
Simone Sommerfeld ◽  
Antonio Vicente Mundim ◽  
Rogério Reis Silva ◽  
Jéssica Santos Queiroz ◽  
Maisa Paschoal Rios ◽  
...  
Keyword(s):  
Experimental Model ◽  
Viral Infections ◽  
Blood Parameters ◽  
Physiological Changes ◽  
Blood Cell Count ◽  
In Ovo ◽  
Chicken Embryos ◽  
Microscopic Damage ◽  
Toxicity Of Drugs ◽  
And Oxidative Stress

Abstract Background: Several works have been developed using the Gallus gallus embryo as an experimental model to study the toxicity of drugs and infections. Studies that seek to standardise the evaluated parameters are needed to better understand and identify the viability of chicken embryos (CE) as an experimental model. Therefore, we sought to verify whether macroscopic, histopathological, blood count, metabolites and/or enzymes changes and oxidative stress in CE of different ages are peculiar to the model. To achieve this goal, in ovo assays were performed injecting a virus (Gammacoronavirus) and two drugs (filgrastim and dexamethasone) that cause known changes in adult chickens and other animals. Results: Embryo response to virus and drug challenges may not occur as expected for adult chickens and even different species. While macroscopic and microscopic damage was visible in the case of viral infections, the white blood cell count and inflammation biomarkers did not change. Filgrastim (FG) testing did not result in the expected effects for CE. On the other hand, with dexamethasone (DX), changes in blood parameters and biomarkers were inherent to the model and depended on the stage of CE development.Conclusions: Our work reinforces the importance of standardization and correct use of the model so that the results of infection, toxicity and pharmacokinetics are recorded.

McN-A-343, a muscarinic agonist, reduces inflammation and oxidative stress in an experimental model of ulcerative colitis

Life Sciences ◽  
2021 ◽  
Vol 272 ◽  
pp. 119194
Author(s):  
Diva de Aguiar Magalhães ◽  
Jalles Arruda Batista ◽  
Stefany Guimarães Sousa ◽  
Jayro dos Santos Ferreira ◽  
Lauanda da Rocha Rodrigues ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ulcerative Colitis ◽  
Experimental Model ◽  
Muscarinic Agonist ◽  
And Oxidative Stress

scholarly journals Silver Nanoparticles as Modulators of Myogenesis-Related Gene Expression in Chicken Embryos

Genes ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 629
Author(s):  
Walaa A. Husseiny ◽  
Abeer A. I. Hassanin ◽  
Adel A. S. El Nabtiti ◽  
Karim Khalil ◽  
Ahmed Elaswad
Keyword(s):  
Silver Nanoparticles ◽  
Placebo Treatment ◽  
Cellular Level ◽  
Breast Muscle ◽  
Transcriptional Level ◽  
Poultry Production ◽  
In Ovo ◽  
Chicken Embryos ◽  
Muscle Tissues ◽  
Myogenic Factor

The present study was conducted to investigate the effects of colloidal nanoparticles of silver (Nano-Ag) on the expression of myogenesis-related genes in chicken embryos. The investigated genes included the members of the myogenic regulatory factors family (MRFs) and myocyte enhancer factor 2A (MEF2A) genes. A total of 200 fertilized broiler eggs (Indian River) were randomly distributed into four groups; non-injected control, injected control with placebo, treatment I in ovo injected with 20 ppm Nano-Ag, and treatment II in ovo injected with 40 ppm Nano-Ag. The eggs were then incubated for 21 days at the optimum temperature and humidity conditions. Breast muscle tissues were collected at the 5th, 8th, and 18th days of the incubation period. The mRNA expression of myogenic determination factor 1 (MYOD1), myogenic factor 5 (MYF5), myogenic factor 6 (MYF6), myogenin (MYOG), and MEF2A was measured at the three sampling points using real-time quantitative PCR, while MYOD1 protein expression was evaluated on day 18 using western blot. Breast muscle tissues were histologically examined on day 18 to detect the changes at the cellular level. Our results indicate that myogenesis was enhanced with the low concentration (20 ppm) of Nano-Ag due to the higher expression of MYOD1, MYF5, and MYF6 at the transcriptional level and MYOD1 at the translational level. Moreover, histological analysis revealed the presence of hyperplasia (31.4% more muscle fibers) in treatment I (injected with 20 ppm). Our findings indicate that in ovo injection of 20 ppm Nano-Ag enhances the development of muscles in chicken embryos compared with the 40-ppm dosage and provide crucial information for the use of silver nanoparticles in poultry production.

Development of specific muscle and cutaneous sensory projections in cultured segments of spinal cord

Development ◽  
1994 ◽  
Vol 120 (5) ◽  
pp. 1315-1323 ◽  
Author(s):  
K. Sharma ◽  
Z. Korade ◽  
E. Frank
Keyword(s):  
Spinal Cord ◽  
Dorsal Horn ◽  
Muscle Afferents ◽  
Cutaneous Afferents ◽  
In Ovo ◽  
Sensory Afferents ◽  
Chicken Embryos ◽  
Sensory Projections ◽  
Spinal Segments ◽  
Horn Development

Development of sensory projections was studied in cultured spinal segments with attached dorsal root ganglia. In spinal segments from stage 30 (E6.5) and older chicken embryos, prelabeled muscle and cutaneous afferents established appropriate projections. Cutaneous afferents terminated solely within the dorsolateral laminae, whereas some muscle afferents (presumably Ia afferents) projected ventrally towards motoneurons. Development of appropriate projections suggests that sufficient cues are preserved in spinal segments to support the formation of modality-specific sensory projections. Further, because these projections developed in the absence of muscle or skin, these results show that the continued presence of peripheral targets is not required for the formation of specific central projections after stage 29 (E6.0). Development of the dorsal horn in cultured spinal segments was assessed using the dorsal midline as a marker. In ovo, this midline structure appears at stage 29. Lack of midline formation in stage 28 and 29 cultured spinal segments suggests that the development of the dorsal horn is arrested in this preparation. This is consistent with earlier reports suggesting that dorsal horn development may be dependent on factors outside the spinal cord. Because dorsal horn development is blocked in cultured spinal segments, this preparation makes it possible to study the consequences of premature ingrowth of sensory axons into the spinal cord. In chicken embryos sensory afferents reach the spinal cord at stage 25 (E4.5) but do not arborize within the gray matter until stage 30. During this period dorsal horn cells are still being generated. In spinal segments, only those segments that have developed a midline at the time of culture support the formation of midline at the time of culture support the formation of specific sensory projections.(ABSTRACT TRUNCATED AT 250 WORDS)

Organisation of Medical Care for Pregnant Women, Women in Labor and New Mothers with COVID-19 Infection

2020 ◽  
pp. 47-56
Author(s):  
Vladimir Klimov
Keyword(s):  
Pregnant Women ◽  
Physiological Condition ◽  
Viral Infections ◽  
Physiological Changes ◽  
Insufficient Information ◽  
Present Stage ◽  
New Mothers ◽  
High Affinity ◽  
Coronavirus Infection ◽  
Respiratory Viral Infections

Although pregnancy is a physiological condition, this category of women has a high probability of severe acute respiratory viral infections, and a fairly high affinity to these diseases, due to physiological changes in the immune system. In 2009/2010, during the A(H1N1)09 virus that caused pig flu, the morbidity among pregnant women reached 27.9%. In addition, such viruses as SARS-CoV and MERS-CoV are known to contribute to severe complications in pregnancy and lead to the need for endotracheal intubation and hospitalisation of women to the intensive care unit, and in some cases, diseases caused by these viruses can cause kidney failure and even death, which reaches 25% when SARS-CoV is detected among pregnant women. At the present stage, there is insufficient information on the new coronavirus infection impact on pregnant women and newborns, and there are no reliable recommendations as to the management of pregnant women when COVID-19 is diagnosed. In this article, we will try to summarise information on managing such patients based on the cases when coronavirus was diagnosed in pregnant women.

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