scholarly journals Silver Nanoparticles as Modulators of Myogenesis-Related Gene Expression in Chicken Embryos

Genes ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 629
Author(s):  
Walaa A. Husseiny ◽  
Abeer A. I. Hassanin ◽  
Adel A. S. El Nabtiti ◽  
Karim Khalil ◽  
Ahmed Elaswad
Keyword(s):  
Silver Nanoparticles ◽  
Placebo Treatment ◽  
Cellular Level ◽  
Breast Muscle ◽  
Transcriptional Level ◽  
Poultry Production ◽  
In Ovo ◽  
Chicken Embryos ◽  
Muscle Tissues ◽  
Myogenic Factor

The present study was conducted to investigate the effects of colloidal nanoparticles of silver (Nano-Ag) on the expression of myogenesis-related genes in chicken embryos. The investigated genes included the members of the myogenic regulatory factors family (MRFs) and myocyte enhancer factor 2A (MEF2A) genes. A total of 200 fertilized broiler eggs (Indian River) were randomly distributed into four groups; non-injected control, injected control with placebo, treatment I in ovo injected with 20 ppm Nano-Ag, and treatment II in ovo injected with 40 ppm Nano-Ag. The eggs were then incubated for 21 days at the optimum temperature and humidity conditions. Breast muscle tissues were collected at the 5th, 8th, and 18th days of the incubation period. The mRNA expression of myogenic determination factor 1 (MYOD1), myogenic factor 5 (MYF5), myogenic factor 6 (MYF6), myogenin (MYOG), and MEF2A was measured at the three sampling points using real-time quantitative PCR, while MYOD1 protein expression was evaluated on day 18 using western blot. Breast muscle tissues were histologically examined on day 18 to detect the changes at the cellular level. Our results indicate that myogenesis was enhanced with the low concentration (20 ppm) of Nano-Ag due to the higher expression of MYOD1, MYF5, and MYF6 at the transcriptional level and MYOD1 at the translational level. Moreover, histological analysis revealed the presence of hyperplasia (31.4% more muscle fibers) in treatment I (injected with 20 ppm). Our findings indicate that in ovo injection of 20 ppm Nano-Ag enhances the development of muscles in chicken embryos compared with the 40-ppm dosage and provide crucial information for the use of silver nanoparticles in poultry production.

scholarly journals In Ovo Administration of Silver Nanoparticles and/or Amino Acids Influence Metabolism and Immune Gene Expression in Chicken Embryos

2015 ◽  
Vol 16 (12) ◽  
pp. 9484-9503 ◽  
Author(s):  
Subrat Bhanja ◽  
Anna Hotowy ◽  
Manish Mehra ◽  
Ewa Sawosz ◽  
Lane Pineda ◽  
...  
Keyword(s):  
Gene Expression ◽  
Amino Acids ◽  
Silver Nanoparticles ◽  
Immune Gene ◽  
In Ovo ◽  
Chicken Embryos ◽  
Immune Gene Expression

scholarly journals Effects of Silver Nanoparticles on Proliferation and Apoptosis in Granulosa Cells of Chicken Preovulatory Follicles: An In Vitro Study

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1652
Author(s):  
Dorota Katarzyńska-Banasik ◽  
Anna Kozubek ◽  
Małgorzata Grzesiak ◽  
Andrzej Sechman
Keyword(s):  
Silver Nanoparticles ◽  
Granulosa Cells ◽  
Caspase 3 ◽  
In Vitro Study ◽  
Poultry Production ◽  
Cell Death Pathways ◽  
Preovulatory Follicles ◽  
Proliferation And Apoptosis ◽  
Apoptosis Process

The continuous development of poultry production related to the growing demand for eggs and chicken meat makes it necessary to use modern technologies. An answer to this demand may be the use of nanotechnology in poultry farming. One of the promising nanomaterials in this field are silver nanoparticles (AgNPs), which are used as disinfectants, reducing microbial pollution and the amounts of greenhouse gases released. This study aimed to evaluate the effect of AgNPs on the proliferation and apoptosis process in the granulosa cells of chicken preovulatory follicles. The in vitro culture experiment revealed that both 13 nm and 50 nm AgNPs inhibited the proliferation of the granulosa cells. However, a faster action was observed in 50 nm AgNPs than in 13 nm ones. A size-dependent effect of AgNP was also demonstrated for the caspase-3 activity. AgNPs 13 nm in size increased the caspase-3 activity in granulosa cells, while 50 nm AgNPs did not exert an effect, which may indicate the induction of distinct cell death pathways by AgNPs. In conclusion, our study reveals that AgNPs in vitro inhibit granulosa cell proliferation and stimulate their apoptosis. These results suggest that AgNPs may disrupt the final stage of preovulatory follicle maturation and ovulation.

Oxidative stress in broiler chicken and its consequences on meat quality

2021 ◽  
Vol 1 (1) ◽  
pp. 045-054
Author(s):  
Ali Hassan Nawaz ◽  
Li Zhang
Keyword(s):  
Oxidative Stress ◽  
Meat Quality ◽  
Poultry Feed ◽  
Poultry Production ◽  
Contributing Factors ◽  
Nutritive Quality ◽  
Muscle Tissues ◽  
Biochemical Status ◽  
Transport Chain ◽  
Processing Techniques

Oxidative stress is inevitable in poultry production, and it affects the physiological, behavioral and biochemical status of growing chicken which ultimately deteriorates meat quality. Appearance, texture, juiciness, tenderness and odor are responsible for the overall meat quality as they are essential perceptible features, which determine the consumer’s judgment. Overproduction of free radicals including reactive oxygen species (ROS) disturbs the mitochondrial function in living cells. During high-temperature mitochondrial substrate oxidation and electron transport chain (ETC) activity increases. This increased activity results in excessive production of superoxide that oxidizes protein and lipid contents in muscle tissues. By oxidizing protein and lipid, ROS spoils the nutritive quality of chicken meat. High ambient temperature is one of the major contributing factors that enhance oxidative stress. Poultry feed with anti-oxidant supplementation and innovative processing techniques can help the poultry industry to overcome oxidative stress.

scholarly journals p38/AP-1 Pathway in Lipopolysaccharide-Induced Inflammatory Responses Is Negatively Modulated by Electrical Stimulation

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Deok Jeong ◽  
Jaehwi Lee ◽  
Young-Su Yi ◽  
Yanyan Yang ◽  
Kyoung Won Kim ◽  
...  
Keyword(s):  
Electrical Stimulation ◽  
Inflammatory Response ◽  
Inflammatory Responses ◽  
Inflammatory Diseases ◽  
Physiological Role ◽  
Peritoneal Macrophages ◽  
Cellular Level ◽  
Transcriptional Level ◽  
Weak Current ◽  
Anti Inflammatory

Electrical stimulation with a weak current has been demonstrated to modulate various cellular and physiological responses, including the differentiation of mesenchymal stem cells and acute or chronic physical pain. Thus, a variety of investigations regarding the physiological role of nano- or microlevel currents at the cellular level are actively proceeding in the field of alternative medicine. In this study, we focused on the anti-inflammatory activity of aluminum-copper patches (ACPs) under macrophage-mediated inflammatory conditions. ACPs generated nanolevel currents ranging from 30 to 55 nA in solution conditions. Interestingly, the nanocurrent-generating aluminum-copper patches (NGACPs) were able to suppress both lipopolysaccharide-(LPS-) and pam3CSK-induced inflammatory responses such as NO and PGE2production in both RAW264.7 cells and peritoneal macrophages at the transcriptional level. Through immunoblotting and immunoprecipitation analyses, we found that p38/AP-1 could be the major inhibitory pathway in the NGACP-mediated anti-inflammatory response. Indeed, inhibition of p38 by SB203580 showed similar inhibitory activity of the production of TNF-αand PGE2and the expression of TNF-αand COX-2 mRNA. These results suggest that ACP-induced nanocurrents alter signal transduction pathways that are involved in the inflammatory response and could therefore be utilized in the treatment of various inflammatory diseases such as arthritis and colitis.

scholarly journals Embryonic Thermal Manipulation and in ovo Gamma-Aminobutyric Acid Supplementation Regulating the Chick Weight and Stress-Related Genes at Hatch

2022 ◽  
Vol 8 ◽  
Author(s):  
Akshat Goel ◽  
Chris Major Ncho ◽  
Chae-Mi Jeong ◽  
Yang-Ho Choi
Keyword(s):  
Gene Expression ◽  
Growth Performance ◽  
Organ Weight ◽  
Aminobutyric Acid ◽  
Poultry Production ◽  
Gamma Aminobutyric Acid ◽  
Related Gene ◽  
In Ovo ◽  
Stress Related Genes ◽  
Thermal Manipulation

Chickens are exposed to numerous types of stress from hatching to shipping, influencing poultry production. Embryonic manipulation may develop resistance against several stressors. This study investigates the effects of thermoneutral temperature (T0; 37.8°C) with no injection (N0) (T0N0), T0 with 0.6 ml of 10% in ovo gamma-aminobutyric acid (GABA) supplementation (N1) at 17.5th embryonic day (ED) (T0N1), thermal manipulation (T1) at 39.6°C from the 10th to 18th ED (6 h/day) with N0 (T1N0), and T1 with N1 (T1N1) on hatchability parameters and hepatic expression of stress-related genes in day-old Arbor Acres chicks. The parameters determined were hatchability, body weight (BW), organ weight, hepatic malondialdehyde (MDA), and antioxidant-related gene expression. Percent hatchability was calculated on a fertile egg basis. Growth performance was analyzed using each chick as an experimental unit. Eight birds per group were used for organ weight. Two-way ANOVA was used taking temperature and GABA as the main effect for growth performance and gene expression studies. Analysis was performed using an IBM SPSS statistics software package 25.0 (IBM software, Chicago, IL, USA). Hatchability was similar in all the groups and was slightly lower in the T1N1. Higher BW was recorded in both T1 and N1. Intestinal weight and MDA were higher in T0N1 against T0N0 and T1N1, respectively. The expression of HSP70, HSP90, NOX1, and NOX4 genes was higher and SOD and CAT genes were lower in the T1 group. The present results show that T1 and N1 independently improve the BW of broiler chicks at hatch, but T1 strongly regulates stress-related gene expression and suggests that both T1 and N1 during incubation can improve performance and alleviate stress after hatch.

Development of specific muscle and cutaneous sensory projections in cultured segments of spinal cord

Development ◽  
1994 ◽  
Vol 120 (5) ◽  
pp. 1315-1323 ◽  
Author(s):  
K. Sharma ◽  
Z. Korade ◽  
E. Frank
Keyword(s):  
Spinal Cord ◽  
Dorsal Horn ◽  
Muscle Afferents ◽  
Cutaneous Afferents ◽  
In Ovo ◽  
Sensory Afferents ◽  
Chicken Embryos ◽  
Sensory Projections ◽  
Spinal Segments ◽  
Horn Development

Development of sensory projections was studied in cultured spinal segments with attached dorsal root ganglia. In spinal segments from stage 30 (E6.5) and older chicken embryos, prelabeled muscle and cutaneous afferents established appropriate projections. Cutaneous afferents terminated solely within the dorsolateral laminae, whereas some muscle afferents (presumably Ia afferents) projected ventrally towards motoneurons. Development of appropriate projections suggests that sufficient cues are preserved in spinal segments to support the formation of modality-specific sensory projections. Further, because these projections developed in the absence of muscle or skin, these results show that the continued presence of peripheral targets is not required for the formation of specific central projections after stage 29 (E6.0). Development of the dorsal horn in cultured spinal segments was assessed using the dorsal midline as a marker. In ovo, this midline structure appears at stage 29. Lack of midline formation in stage 28 and 29 cultured spinal segments suggests that the development of the dorsal horn is arrested in this preparation. This is consistent with earlier reports suggesting that dorsal horn development may be dependent on factors outside the spinal cord. Because dorsal horn development is blocked in cultured spinal segments, this preparation makes it possible to study the consequences of premature ingrowth of sensory axons into the spinal cord. In chicken embryos sensory afferents reach the spinal cord at stage 25 (E4.5) but do not arborize within the gray matter until stage 30. During this period dorsal horn cells are still being generated. In spinal segments, only those segments that have developed a midline at the time of culture support the formation of midline at the time of culture support the formation of specific sensory projections.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals In ovo feeding improves energy status of late-term chicken embryos

2005 ◽  
Vol 84 (5) ◽  
pp. 764-770 ◽  
Author(s):  
Z. Uni ◽  
P.R. Ferket ◽  
E. Tako ◽  
O. Kedar
Keyword(s):  
Energy Status ◽  
In Ovo ◽  
Chicken Embryos ◽  
In Ovo Feeding

scholarly journals Betaine and Antioxidants Improve Growth Performance, Breast Muscle Development and Ameliorate Thermoregulatory Responses to Cyclic Heat Exposure in Broiler Chickens

Animals ◽  
2018 ◽  
Vol 8 (10) ◽  
pp. 162 ◽  
Author(s):  
Majid Shakeri ◽  
Jeremy Cottrell ◽  
Stuart Wilkinson ◽  
Mitchell Ringuet ◽  
John Furness ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Muscle Development ◽  
Average Daily Gain ◽  
Heat Exposure ◽  
Basal Diet ◽  
Breast Muscle ◽  
Broiler Chicks ◽  
Poultry Production ◽  
Feed Conversion ◽  
Muscle Weight

Heat stress (HS) is an environmental stressor challenging poultry production and requires a strategy to cope with it. A total of 288-day-old male broiler chicks were fed with one of the following diets: basal diet, basal with betaine (BET), or with selenium and vitamin E (AOX), or with a combination of BET and AOX, under thermoneutral and cyclic HS. Results showed that HS reduced average daily feed intake (ADFI) (p = 0.01) and average daily gain (ADG) (p < 0.001), and impaired feed conversion ratio (FCR) (p = 0.03) during rearing period (0–42 day). BET increased ADG (p = 0.001) and decreased FCR (p = 0.02), whereas AOX had no effects. Breast muscle weight was decreased by HS (p < 0.001) and increased by BET (p < 0.001). Rectal temperature was increased by HS (p < 0.001) and improved by BET overall. Respiration rate was increased by HS (p < 0.001), but BET decreased it during HS (p = 0.04). Jejunum transepithelial resistance was reduced by HS and had no effect on permeability whereas BET increased jejunum permeability (p = 0.013). Overall, the reductions in ADG of broiler chickens during HS were ameliorated by supplementation with BET, with much of the increase in ADG being breast muscle.

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