Establishment and Functional Characterization of Immortalized Rabbit Dermal Papilla Cell Lines

2021 ◽  
Author(s):  
Jiali Li ◽  
Bohao Zhao ◽  
Chen Zhang ◽  
Xiyu Zhang ◽  
Yingying Dai ◽  
...  
Keyword(s):  
Cell Lines ◽  
Growth And Development ◽  
Functional Characterization ◽  
Dermal Papilla ◽  
Early Passage ◽  
Dermal Papilla Cells ◽  
Viability Analysis ◽  
Periodic Growth ◽  
Basic Characteristics ◽  
Immortalized Cell

Abstract Background Hair follicle (HF) undergo periodic growth and development in mammals, which regulated by dermal papilla cells (DPCs) are reported to play an important role in the HF morphogenesis and development. However, primary DPCs have low proliferative activity, age quickly, and fresh cell isolation is both time-consuming and laborious. Method In this study, we introduced SV40LT into dissociated early passage rabbit vibrissae DPCs with lentiviral vectors and established seven immortalized DP cell lines (R-1, R-2, R-3, R-4, R-5, R-6 and R-7). Result These cell lines displayed early passage morphology and displayed high alkaline phosphatase activity. RT-PCR and immunofluorescence staining showed that all the immortalized cell lines expressed the DPC markers (α-SMA ,IGF1, ALPL, FGF2, BMP2 and TGFβ2; α-SMA and VIM protein), but α-SMA was only expressed well in R-3, R-4, and R-7. Furthermore, it was found that R-7 was the only line to survive beyond 50 passages. Compared to melanoma cells, R-7 did not undergo malignant transformation. Karyotyping and cell growth viability analysis illustrated that the R-7 cell line preserved the basic characteristics of primary DPCs. Conclusion The R-7 DPCs established have potential application for future hair research. The study provides the theoretical basis in the cell research of HF growth and development.

Transfection of rat dermal papilla cells with a gene encoding a temperature-sensitive polyomavirus large T antigen generates cell lines retaining a differentiated phenotype

1994 ◽  
Vol 107 (7) ◽  
pp. 1761-1772
Author(s):  
W. Filsell ◽  
J.C. Little ◽  
A.J. Stones ◽  
S.P. Granger ◽  
S.A. Bayley
Keyword(s):  
Cell Lines ◽  
Hair Growth ◽  
Dermal Papilla ◽  
Growth Cycle ◽  
T Antigen ◽  
Temperature Sensitive ◽  
Early Passage ◽  
Gene Encoding ◽  
Dermal Papilla Cells ◽  
Dermal Papilla Cell

The dermal papilla is a discrete group of cells at the base of the hair follicle and is implicated in controlling the hair growth cycle. Early passage dermal papilla cells can induce hair growth in vivo, but, upon further culturing, this property is lost. In order to study the events occurring in hair induction, a representative dermal papilla cell line was required. We have transfected passage 1 rat vibrissa dermal papilla cells with a polyomavirus large T gene encoding a temperature-sensitive T antigen, and generated permanent cell lines in which the immortalizing function can be switched off by temperature shift. The cells established without crisis, resembled cells in the starting population, and retained the aggregative properties of early passage dermal papilla cells. Growth studies were performed on the immortalized cell lines, which showed that transferring the cells to the restrictive temperature for the large T gene product resulted in cell senescence or quiescence, and changes in morphology. Implantation of cell pellets into the ears of immunologically compatible rats showed that the immortal cells retained hair-inductive ability. Cytokines are believed to have an important role in the control of hair growth. The pattern of cytokine gene expression in the immortal cell lines was compared with early passage dermal papilla cells and a non-hair-inducing dermal papilla cell line, using reverse transcriptase-polymerase chain reaction. Epidermal growth factor, tumour necrosis factor, and interleukin-1a were detected in the immortalized and non-hair-inducing dermal papilla cell lines, but were absent in passage 2 dermal papilla cells. All other cytokines examined were detected in all the cell types under study. These results demonstrate that the polyomavirus large Ttsa-immortalized dermal papilla cell lines are very similar to passage 2 dermal papilla cells and thus provide a good model for hair growth studies. Cytokine expression profiles indicate that the expression of several cytokines may be implicated in hair induction. Further studies are under way to investigate the relationship between cytokine expression and the hair growth cycle.

scholarly journals Comparison of Effects on Gene Expression Activity of Low-Molecular-Weight Lychee Fruit Polyphenol (Oligonol®), Adenosine, and Minoxidil in Human Dermal Papilla Cells

2017 ◽  
Vol 3 (1) ◽  
pp. 5 ◽  
Author(s):  
Koji Wakame ◽  
Akifumi Nakata ◽  
Keisuke Sato ◽  
Yoshihiro Mihara ◽  
Jun Takanari ◽  
...  
Keyword(s):  
Cell Lines ◽  
Dna Microarrays ◽  
Food Product ◽  
Dermal Papilla ◽  
Pcr Analysis ◽  
Low Molecular Weight ◽  
Dermal Papilla Cells ◽  
Dermal Papilla Cell ◽  
Expression Of Genes ◽  
Expression Activity

Background: Oligonol® (OLG) is a functional food product and ingredient for cosmetics derived from a lychee fruit polyphenol. It has been reported to act on the skin as an anti-inflammatory and prevent UVB-induced skin damage.Aim: In this study, with the aim of exploring new functionalities of OLG on the scalp, we investigated the effect of OLG on human dermal papilla cells by comparing with adenosine and minoxidil at the genetic level.Method: OLG, adenosine, and minoxidil were applied to human dermal papilla cell lines for 24 h, after which VEGF, FGF-7, WNT5a, and WNT10a mRNA expressions were measured by real-time PCR analysis. Additionally, using DNA microarrays, we investigated the effect on 205 inflammation-related genes.Result: Consequently, in human dermal papilla cell lines, FGF-7 and WNT10a mRNA expression were observed in 100 µg/mL OLG-supplemented cells. The results of the DNA microarray analysis showed that 10 genes were suppressed by OLG.Conclusions: OLG may be expected to affect function of human dermal papilla cell by regulating the expression of genes related to cell proliferation and inflammation.

scholarly journals miR-195-5p Regulates Hair Follicle Inductivity of Dermal Papilla Cells by Suppressing Wnt/β-Catenin Activation

2018 ◽  
Vol 2018 ◽  
pp. 1-13 ◽  
Author(s):  
Ningxia Zhu ◽  
Keng Huang ◽  
Yang Liu ◽  
Huan Zhang ◽  
En Lin ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Posttranscriptional Regulation ◽  
Dermal Papilla ◽  
Vital Role ◽  
Early Passage ◽  
Dermal Papilla Cells ◽  
Key Factor ◽  
Late Passage ◽  
Wnt Pathways ◽  
Canonical Wnt

Dermal papilla (DP) cells play a vital role in hair follicle (HF) development and postnatal hair cycling. However, the abilities are lost on further culture. Recent studies have demonstrated significant influences of posttranscriptional regulation by microRNA (miRNA) on HF development. The current study aims to investigate how miRNAs regulate Wnt/β-catenin to control HF inductivity of DP cells by performing microarray analysis in early- and late-passage DP cells and transfecting with miRNAs inhibitor or mimic. Results showed early-passage DP cells strongly expressed miRNAs related to inhibition of noncanonical Wnt pathways. In late-passage DP cells, miRNAs capable of inhibiting the canonical Wnt/β-catenin pathway were upregulated, in addition to the miRNAs targeting the noncanonical Wnt pathway. Moreover, we verified that β-catenin expression was downregulated by miR-195-5p overexpression in dose manner. Meanwhile LRP6 expression was downregulated in both protein and mRNA as well as the genes involved in the hair inductivity of DP cells. These results suggest that the appearance of miRNAs that suppress the Wnt/β-catenin pathway may be responsible for the loss of ability of DP cells in culture and miR-195-5p is the potential key factor involved in regulating HF inductivity of DP cells.

Whisker growth induced by implantation of cultured vibrissa dermal papilla cells in the adult rat

Development ◽  
1986 ◽  
Vol 97 (1) ◽  
pp. 111-124
Author(s):  
Kenneth A. Horne ◽  
Colin A. B. Jahoda ◽  
Roy F. Oliver
Keyword(s):  
Hair Growth ◽  
Cell Activity ◽  
Dermal Papilla ◽  
Growth Cycle ◽  
Early Passage ◽  
Dermal Papilla Cells ◽  
Adult Rat ◽  
Late Passage ◽  
Papilla Formation ◽  
Spontaneous Regeneration

Retention of the capacity to induce the growth of hair by cultured adult rat vibrissa dermal papilla cells has been investigated. Small pellets of serially cultured papilla cells were implanted into the bases of the exposed follicular epidermis of amputated adult rat vibrissa follicles. Amputated follicles that received no cell implants or implants of cultured dorsal skin fibroblasts were used as controls. Over 50% of follicles implanted with cultured papilla cells in the passage range 1–3 grew hairs. In contrast none of the follicles that received late passage cells (range 6–15) produced hairs; and spontaneous regeneration of hair occurred in only 3% of the control follicles. These results demonstrate that cultured papilla cells of early passage numbers retain their ability to induce hair growth. Histological examination confirmed that the implanted papilla cells interacted with follicular epidermis to organize the development of new, hair-producing bulbs, each containing a discrete dermal papilla. An important observation was that aggregative behaviour leading to papilla formation was only manifested by early passage papilla cell implants. This persisting embryonic characteristic appears to be an essential functional component of papilla cell activity which operates to regulate the profound morphogenetic changes that occur during the hair growth cycle.

scholarly journals Detecting the Mechanism behind the Transition from Fixed Two-Dimensional Patterned Sika Deer (Cervus nippon) Dermal Papilla Cells to Three-Dimensional Pattern

2021 ◽  
Vol 22 (9) ◽  
pp. 4715
Author(s):  
Guanning Wei ◽  
Hongmei Sun ◽  
Haijun Wei ◽  
Tao Qin ◽  
Yifeng Yang ◽  
...  
Keyword(s):  
Culture Condition ◽  
Sika Deer ◽  
Three Dimensional ◽  
Cervus Nippon ◽  
Dermal Papilla ◽  
Growth Patterns ◽  
The Self ◽  
Dermal Papilla Cells ◽  
Aggregative Behavior ◽  
Cell Pattern

The hair follicle dermal papilla is critical for hair generation and de novo regeneration. When cultured in vitro, dermal papilla cells from different species demonstrate two distinguishable growth patterns under the conventional culture condition: a self-aggregative three dimensional spheroidal (3D) cell pattern and a two dimensional (2D) monolayer cell pattern, correlating with different hair inducing properties. Whether the loss of self-aggregative behavior relates to species-specific differences or the improper culture condition remains unclear. Can the fixed 2D patterned dermal papilla cells recover the self-aggregative behavior and 3D pattern also remains undetected. Here, we successfully constructed the two growth patterns using sika deer (Cervus nippon) dermal papilla cells and proved it was the culture condition that determined the dermal papilla growth pattern. The two growth patterns could transit mutually as the culture condition was exchanged. The fixed 2D patterned sika deer dermal papilla cells could recover the self-aggregative behavior and transit back to 3D pattern, accompanied by the restoration of hair inducing capability when the culture condition was changed. In addition, the global gene expressions during the transition from 2D pattern to 3D pattern were compared to detect the potential regulating genes and pathways involved in the recovery of 3D pattern and hair inducing capability.

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