Bioaugmentation of Corn Stalks Saccharification with Aspergillus Fumigatus Under Low/High Solid Loading Culture
Abstract Decomposed the dense structure of lignocellulosic feedstocks and hydrolysis lignocellulose into monosaccharide were essential prerequisite for bio-energy production at this level. In this study, a cellulosic fungi Aspergillus fumigatus CLL was conducted to pretreated the corn stalks under high/low solid loading culture to enhanced the cellulase saccharification performance. The results indicated that A. fumigatus CLL decomposed the corn stalks effectively under high/low solid loading culture, what’s more, A. fumigatus CLL completed the T. reesei cellulase system and promoted the corn stalks saccharification performance. 25.2% lignin was degraded after A. fumigatus CLL treated just for two day under low solid loading culture with holocellulose loss less than 10%. Meanwhile, the β-glucosidase of A. fumigatus CLL complemented the incomplete cellulase system of T. reesei, the maximum saccharification ratio of sample saccharified by T. reesei cellulase combined A. fumigatus CLL was comparable with the sample saccharified by commercial cellulase. Compared with raw corn stalks, the saccharification ratio of pretreated sample increased 3.1-3.4 fold. These results demonstrated that A. fumigatus CLL can be used for pretreatment of lignocellulosic materials to enhanced the saccharification performance.