Does spontaneous eradication of Helicobacter pylori infection lead to functional dyspepsia?

Abstract Introduction: The treatment of patients with functional dyspepsia in cases of active helicobacter pylori (H. pylori) infection is not promising. Therefore, the present study was designed to determine in which of the dyspeptic patients with the normal endoscopic examination and negative stool testing for H. pylori antigen (without a history of specific treatment for H. pylori) there is evidence of a previous H. pylori infection in the serum.Materials and methods: In this cross-sectional study, patients with functional dyspepsia who were negative in terms of the H. pylori stool antigen test and rapid urease test, and had no obvious gross pathologic sign in gastroduodenoscopy were considered suitable candidates for serological study for the detection of H. pylori IgG specific antibodies.Results: A total of 200 patients were enrolled in this study, including 86 men (43%) and 114 women (57%), with an average age of 38.76 ± 12.35 years. The results showed that 109 (54.5%) were positive subjects were positive by anti-H. pylori IgG ELISA tests. It was found that positive anti-H. pylori IgG ELISA tests were a higher significant difference among rural residents (75.2%) compared with urban residents (24%) (p<0.001).Conclusion: More than half of patients with functional dyspepsia have a history of previous H.pylori infection. Despite the possibility of spontaneous H. pylori infection, the complication of old infections may be sustained by dyspepsia and early treatment of acute H. pylori infections may prevent this complication.

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Comparison of IL-6, IL-8 Concentrations in H. pylori- and non-H. pylori-associated Gastritis

The Indonesian Biomedical Journal ◽

10.18585/inabj.v6i3.29 ◽

2014 ◽

Vol 6 (3) ◽

pp. 163 ◽

Cited By ~ 1

Author(s):

Gontar Alamsyah Siregar ◽

Sahat Salim ◽

Ricky Rivalino Sitepu

Keyword(s):

Helicobacter Pylori ◽

Serum Levels ◽

Cross Sectional Study ◽

Bivariate Analysis ◽

Rapid Urease Test ◽

Serum Samples ◽

Cross Sectional ◽

Non Invasive ◽

Urease Test ◽

H Pylori

BACKGROUND: Helicobacter pylori is a non-invasive microorganism causing intense gastric mucosal inflammatory and immune reaction. The gastric mucosal levels of the proinflammatory cytokines Interleukin 6 (IL-6) and IL-8 have been reported to be increased in H. pylori infection, but the serum levels in H. pylori infection is still controversial. The purpose of this study was to investigate the serum levels of IL-6 and IL-8 in H. pylori infection.METHODS: A cross sectional study was done on eighty consecutive gastritis patients admitted to endoscopy units at Adam Malik General Hospital and Permata Bunda Hospital, Medan, Indonesia from May-October 2014. Histopathology was performed for the diagnosis of gastritis. Rapid urease test for diagnosis of H. pylori infection. Serum samples were obtained to determine circulating IL-6 and IL-8. Univariate and bivariate analysis (independent t test) were done.RESULTS: There were 41.25% patients infected with H. pylori. Circulatory IL-6 levels were significantly higher in H. pylori-infected patients compared to H. pylori negative, but there were no differences between serum levels of IL-8 in H. pylori positive and negative patients.CONCLUSION: The immune response to H. pylori promotes systemic inflammation, which was reflected in an increased level of serum IL-6. Serum levels of IL-8 were not significantly different between H. pylori positive and negative.KEYWORDS: Helicobacter pylori, gastritis, IL-6, IL-8, cytokine

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GENDER, AGE, ENDOSCOPIC FINDINGS, UREASE AND HELICOBACTER PYLORI: ALL UNCORRELATED WITHIN A SAMPLE OF A HIGH GASTRIC CANCER PREVALENCE POPULATION IN AMAZON

Arquivos de Gastroenterologia ◽

10.1590/s0004-2803.201900000-50 ◽

2019 ◽

Vol 56 (3) ◽

pp. 264-269

Author(s):

Ariney Costa de MIRANDA ◽

Cássio CALDATO ◽

Mira Nabil SAID ◽

Caio de Souza LEVY ◽

Claudio Eduardo Corrêa TEIXEIRA ◽

...

Keyword(s):

Gastric Cancer ◽

Helicobacter Pylori ◽

Cross Sectional Study ◽

Upper Gastrointestinal Endoscopy ◽

Histopathological Analysis ◽

Rapid Urease Test ◽

Cross Sectional ◽

Cancer Prevalence ◽

Urease Test ◽

H Pylori

ABSTRACT BACKGROUND: It is widely assumed that gender, age, gastritis and Helicobacter pylori , all have some degree of correlation and, therefore, can synergistically lead to the development of gastric cancer. OBJECTIVE: In this cross-sectional study, we expected to observe the above mentioned correlation in the analysis of medical records of 67 patients of both sexes (female, n=44), mean age ± standard deviation: 41±12 years old, all from Belém (capital of Pará State, Brazilian Amazon), a city historically known as one with the highest gastric cancer prevalence in this country. METHODS: All patients were submitted to upper gastrointestinal endoscopy for gastric biopsy histopathological analysis and rapid urease test. All diagnoses of gastritis were recorded considering its topography, category and the degree of inflammatory activity, being associated or not associated with H. pylori infection. RESULTS: The results show that no statistically relevant associations were found among the prevalences of the observed variables. CONCLUSION: The authors hypothesize that observed risk factors associated to gastric cancer might be lesser synergistic than is usually expected.

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Helicobacter pylori infection in patients undergoing upper endoscopy at University Hospital in Uberaba, Minas Gerais, Brazil.

JMPHC | Journal of Management & Primary Health Care | ISSN 2179-6750 ◽

10.14295/jmphc.v4i1.164 ◽

2013 ◽

Vol 4 (1) ◽

pp. 33-35 ◽

Cited By ~ 1

Author(s):

Fernanda Machado Fonseca ◽

Renata Margarida Etchebehere ◽

Adriana Gonçalves Oliveira

Keyword(s):

Helicobacter Pylori ◽

Breath Test ◽

Histological Study ◽

Rapid Test ◽

University Hospital ◽

Rapid Urease Test ◽

Duodenal Ulcers ◽

Urease Test ◽

Significant Difference ◽

H Pylori

Helicobacter pylori is a Gram negative bacterium that cause chronic gastritis, duodenal ulcers and can predispose the gastric cancer. The study aimed to determinate the prevalence of H. pylori infection by different methods of diagnosis in patients submitted to endoscopy. Of the 145 patients included in the study, were collected fragments of gastric mucosa for histological analysis, and for the rapid urease test. The breath test was also performed. The H. pylori infection was detected in 84 (57.9%) patients by histological study, the rapid test of urease was positive in 81 (55,8%) and the breath test in 62 (56,3%). There was no statistically significant difference when comparing the prevalence of infection by different methods of diagnosis. The prevalence of H. pylori infection in our community was lower than that found in the literature for patients with age similar to this study (mean = 53.19 years).

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A REVIEW OF HELICOBACTER PYLORI INFECTION DISEASES, ANTIBIOTIC RESISTANCE AND DIAGNOSIS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i12.24745 ◽

2018 ◽

Vol 11 (12) ◽

pp. 566

Author(s):

Bolai Paul ◽

Senthil Adimoolam ◽

Mohd Javed Qureshi ◽

Nahlah Elkudssiah Ismail

Keyword(s):

Helicobacter Pylori ◽

Antibiotic Resistance ◽

Developed Countries ◽

Epidemiologic Studies ◽

Diagnostic Methods ◽

Gastric Ulcers ◽

Rapid Urease Test ◽

Stool Antigen Test ◽

Urease Test ◽

H Pylori

Objective: The aim of the study was designed to assess the mechanisms of antibiotic resistance in Helicobacter pylori, affecting disease by this infection and diagnostic methods which are used to detect H. pylori.Methods: A wide literature search was performed using PubMed, Medline, Cinahl, Embase, Educational Resources Information Center, PsycINFO, Google Scholar, Scopus, and Web of Science, and review of appropriate epidemiologic studies conducted from 1995 to 2017 for studies fully published investigating a contribution between H. pylori infection, antibiotic resistance, and diagnosis of H. pylori infection.Results: H. pylori infection is extremely contributed to the main symptoms and death that is currently affecting 50–75% of the people in the world. It is more affected in developing countries compared to developed countries. These infections are regarded to be the most important reasons for gastric cancer, peptic ulcer, chronic gastritis, duodenal ulcer, mucosa-associated lymphoid tissue lymphomas, and gastric adenocarcinoma. About 90–100% of duodenal ulcers and 60–90% of gastric ulcers were associated with H. pylori infections. At present, antibiotic resistance is a growing problem for the eradication of H. pylori infection; it contains metronidazole, amoxicillin, clarithromycin, and levofloxacin resistance. Diagnosis of H. pylori infection is a crucial part for the better treatment of those diseases. Different types of testing method for H. pylori infection are used including invasive (endoscopic image, histology, rapid urease test, and culture) and non-invasive (urea breath test, stool antigen test, and serological).Conclusion: H. pylori antibiotic resistance is the major contributor to the failure of H. pylori treatment. Appropriate diagnostic method selected in detecting H. pylori antibiotic resistance may lead to reduced treatment failures and less antibiotic resistance.

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Influence of Living Environment during Childhood on Helicobacter pylori Infection in Japanese Young Adults

Digestion ◽

10.1159/000502574 ◽

2019 ◽

Vol 101 (6) ◽

pp. 779-784 ◽

Cited By ~ 1

Author(s):

Takunori Ueno ◽

Hideo Suzuki ◽

Mitsuaki Hirose ◽

Takashi Shida ◽

Kazuto Ikezawa ◽

...

Keyword(s):

Helicobacter Pylori ◽

Young Adult ◽

Statistical Significance ◽

Living Environment ◽

Well Water ◽

Rapid Urease Test ◽

Urease Test ◽

History Of ◽

H Pylori ◽

Usage Rates

Introduction: Helicobacter pylori infection is usually established during childhood, for which certain responsible environmental factors have been identified. However, the details of the infection routes remain unclear. Objective: To determine the relation between H. pylori infection statuses and living environment of Japanese young adult. Methods: The subjects were 449 healthy young adult medical students of Tsukuba University (299 men and 150 women, mean age: 22.8 years). The H. pylori infection statuses were investigated using the rapid urease test or urine antibody. Questionnaires regarding sanitary conditions including usage of pit toilet or well water and experience of living with one’s grandparents during childhood were surveyed. Each item was compared between the H. pylori-positive and -negative groups. Results: Among all participants, 33 (7.3%) were H. pylori-positive. The usage rates of pit toilets were 12.1 and 3.1% for the H. pylori-positive and -negative groups respectively (p = 0.03; OR 4.35, 95% CI 1.33–14.22). The usage rates of well water were 24.2 and 13.7% for the H. pylori-positive and -negative groups respectively (p = 0.07; OR 2.12, 95% CI 0.91–4.98). The proportion of participants with a history of living with their grandparents was significantly greater in the H. pylori-positive group (46.7%) than in the -negative group (20.9%; p = 0.03; OR 3.28, 95% CI 1.13–9.54). Only a history of living with one’s grandparents during childhood showed statistical significance in the multivariate regression analysis (p = 0.04; OR 3.20, 95% CI 1.08–9.49). Conclusions: These results suggest that H. pylori infection is more strongly related to living with one’s grandparents than living in a hygienic environment.

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DIAGNOSTIC UTILITY OF THE RAPID UREASE TEST FOR HELICOBACTER PYLORI IN GASTRIC JUICE SAMPLES

International Journal of Advanced Research ◽

10.21474/ijar01/13225 ◽

2021 ◽

Vol 9 (08) ◽

pp. 32-36

Author(s):

Marlene Ordonez Pereira ◽

◽

Julian David Martinez ◽

Keyword(s):

Helicobacter Pylori ◽

Gastric Juice ◽

Diagnostic Tests ◽

Diagnostic Performance ◽

Medical Center ◽

Rapid Urease Test ◽

Test Device ◽

Cross Sectional ◽

Urease Test ◽

H Pylori

Objective: The purpose of this study is to compare the diagnostic performance of the rapid urease test for H. pylori in endoscopic samples of gastric juice compared with the same test in gastric mucosa. Materials and methods: Cross-sectional, comparative and prospective study with study of concordance of diagnostic tests, carried out with patients referred to a medical center in BogotÃƒÂƒÃ‚Â¡ DC, Colombia for the performance of an esophagogastroduodenoscopy. We included 130 patients older than 18 years, without antibiotic treatment or inhibitors of gastric secretion, or with any type of immunodeficiency, or cancer. Were processed in the sensibacterpyroli test device a sample of gastric antrum for Rapid Ureasa Test RUT in mucosa and for the sample of juice 5 mL was taken with suctionator 7A-23B pulmomed (USA) R, to compare results. Results: In this study the infection by Helicobacter pylori was detected by biopsy sample in 40.8% of the patients that corresponded to 53 patients, among (59.2%) 77 patients with negative values. The prevalence of H. pylori infection in this study was 40.8%. It was calculated of VPP and NPV: 95% and NPV: 89.3%. Conclusions: rapid urease test in gastric juice is a fast and economic test, with very good sensitivity and specificity, is also very useful to detect H. pylori infection.

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Prevalence of Helicobacter Pylori Genotypes and Their Association with Upper Gastrointestinal Diseases: A Cross Sectional Study in Southern Iran

10.21203/rs.3.rs-137967/v2 ◽

2021 ◽

Author(s):

Rahim Raufi ◽

Reza Shahriarirad ◽

SeyedehMaryam Pishva ◽

Nikta Taghipour

Keyword(s):

Helicobacter Pylori ◽

Hiatal Hernia ◽

Gastrointestinal Diseases ◽

Cross Sectional Study ◽

Rapid Urease Test ◽

Cross Sectional ◽

Southern Iran ◽

Normal Esophagus ◽

Urease Test ◽

H Pylori

Abstract Background: Investigating the prevalence of vacuolating cytotoxin (vacA), cytotoxin associated gene A (cagA), glm M genotypes, and subtypes of vacA of Helicobacter pylori (H. pylori) isolate in Jahrom, Southern Iran.Methods: DNA extracted from H. pylori samples retrieved from gastric biopsy isolated from 113 dyspeptic patients with positive rapid urease test (RUT). Genotyping was done by polymerase chain reaction (PCR) technique, using primers for vacA (s1a, s1b, s1c, s1, s2, m2, and m1), cagA, and glmM. Endoscopy was done for all the patients to screen gastrointestinal (GI) pathologies. Results: GlmM was detected in 100% of the cases. VacA subtypes s1am2, s2m2, s1am1, s1b, and s1c were detected in 27.9%, 25.6%, 22.1%, 3.5% and 2.4% of the isolates, respectively, while cagA was detected in 60.5% of the isolates. VacA alleles m1, s1, and s2 were detected in 54%, 50%, and 44% of isolates, respectively. Also, 60.5% of the isolates were cagA-vacA-positive. A significant correlation was observed between vacAs1bm1 and gastroesophageal reflux disease (GERD), and glmM positive isolates had normal esophagus. The presence of vacAs1bm1 and vacAs1bm2 has a significant association with gastric erythema. The presence of cagA showed a significant association with normal esophagus and hiatal hernia.Conclusion: In our research, the number of glmM and cagA positive isolates is higher among other genotypes, and cagA is correlated with hiatal hernia, and normal esophageal finding is correlated with glmM genotype. There was no association between the age or sex of the patients and bacterial genotype.

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Utility of Rapid urease test in diagnosis of Helicobacter pylori infection

International Journal Of Medical Science And Clinical Invention ◽

10.18535/ijmsci/v6i4.05 ◽

2019 ◽

pp. 4417-4422

Author(s):

Shaan Khetrapal ◽

Samarth Shukla ◽

Safia Rana ◽

Zeeba S Jairajpuri ◽

Sourya Acharya

Keyword(s):

Helicobacter Pylori ◽

Cross Sectional Study ◽

Helicobacter Pylori Infection ◽

Rapid Urease Test ◽

Cross Sectional ◽

Intestinal Lesions ◽

Common Diagnosis ◽

Urease Test ◽

H Pylori ◽

Haematoxylin And Eosin Stain

Aim: To evaluate the role of rapid urease test in diagnosis of Helicobacter pylori infection in various gastro intestinal lesions vis a vis conventional and special staining methods. Materials & Methods: This hospital based cross sectional study was conducted on 50 cases with gastro intestinal lesions. The obtained biopsies were then subjected to rapid urease test for detection of H. pylori by using the Pronto Dry Kit immediately. Further histopathology examination using conventional H & E (haematoxylin and eosin) stain and special Gimenez stain for H. pylori was done. Results: In this study gender wise distribution of patients showed 58% males and 42% females. Chronic gastritis was found to be the most common diagnosis (28%), followed by squamous cell carcinoma (20%) chronic inflammation (16%), H. pylori gastritis and adenocarcinoma stomach (6%), adenocarcinoma esophagus, dysplasia, erosion, esophagitis and perforation in 4%. Least common diagnosis was atrophic gastritis and carcinoid in 2%. We found that 26 cases were positive for H. pylori with Rapid urease test and 24 with special stain (Gimenez). Conclusion: This study amalgamates and incorporates the valuable clinical assessment along with the endoscopic findings, the surgical pathological (histopathology) evaluation of the biopsies along with the proto dry kit (rapid urease test), as a kind of “Gastric Triple Test” for declaring the patients as positive or negative for pathogenic infection with Helicobacter pylori.

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Serum VEGF Levels in Helicobacter pylori Infection and Correlation with Helicobacter pylori cagA and vacA Genes

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2017.031 ◽

2017 ◽

Vol 5 (2) ◽

pp. 137-141

Author(s):

Gontar Siregar ◽

Dina Sari ◽

Taufik Sungkar

Keyword(s):

Helicobacter Pylori ◽

Serum Levels ◽

Cross Sectional Study ◽

Rapid Urease Test ◽

Serum Samples ◽

Cross Sectional ◽

Urease Test ◽

H Pylori ◽

Endothelial Growth Factor ◽

Vegf Level

BACKGROUND: Helicobacter pylori vacA and cagA genes are associated with higher virulence. Vascular Endothelial Growth Factor (VEGF) is one important marker for neo-angiogenesis.AIM: The purpose of this study was to investigate the relationship between VEGF serum levels with cagA and vacA genes in H. pylori infection.METHODS: A cross-sectional study was done on eighty patients that consecutive admitted to endoscopy unit. The diagnosis of H. pylori infection was based on rapid urease test. Serum samples were obtained to determine circulating VEGF level. Polymerase chain reaction was done to examine H. pylori vacA and cagA genes. Data analysis were carried-out using SPSS version 22. RESULTS: A total of 80 patients were examined. There were 45 (56.3%) patients infected with Helicobacter pylori. There were 33 (73.3%) patients with H. pylori cagA positive. Serum VEGF levels in patients with the H. pylori positive were significantly higher compared to the patients that have no H. pylori. Serum levels of VEGF were significantly higher in cagA positive than negative.CONCLUSION: Serum VEGF level is correlated with H. pylori infection and its virulence status. The more virulence of H. pylori, cagA gene, the higher serum VEGF levels were found.

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Prevalence of Helicobacter Pylori Genotypes and Their Association With Upper Gastrointestinal Diseases: a Cross Sectional Study in Southern Iran

10.21203/rs.3.rs-137967/v1 ◽

2021 ◽

Author(s):

Rahim Raufi ◽

Reza Shahriarirad ◽

SeyedehMaryam Pishva ◽

Nikta Taghipour

Keyword(s):

Helicobacter Pylori ◽

Hiatal Hernia ◽

Gastrointestinal Diseases ◽

Rapid Urease Test ◽

Cross Sectional ◽

Southern Iran ◽

Normal Esophagus ◽

Urease Test ◽

H Pylori ◽

Upper Gastrointestinal

Abstract Background: Investigating the prevalence of vacuolating cytotoxin (vacA), cytotoxin associated gene A (cagA), glm M genotypes, and subtypes of vacA of Helicobacter pylori (H. pylori) isolate in Jahrom, Southern Iran. DNA extracted from H. pylori samples retrieved from gastric biopsy isolated from 113 dyspeptic patients with positive rapid urease test (RUT). Genotyping was done by polymerase chain reaction (PCR) technique, using primers for vacA (s1a, s1b, s1c, s1, s2, m2, and m1), cagA, and glmM. Endoscopy was done for all the patients to screen upper gastrointestinal (GI) disorders. Results: GlmM was detected in 100% of the cases. VacA subtypes s1am2, s2m2, s1a, s1b, and s1c were detected in 27.9%, 25.6%, 50%, 3.5% and 2.4% of the isolates, respectively, while cagA was detected in 60.5% of the isolates. VacA alleles m1, s1, and s2 were detected in 54%, 50%, and 44% of isolates respectively. Also, 60.5% of the isolates were cagA-vacA-positive. A significant correlation was observed between vacAs1bm1 and gastroesophageal reflux disease (GERD) and glmM and normal esophagus. The presence of vacAs1bm1 and vacAs1bm2 has a significant association with gastric erythema. The presence of cagA showed a significant association with normal esophagus and hiatal hernia.Conclusions: In our research, the number of glmM and cagA positive isolates is higher among other genotypes and cagA is correlated with hiatal hernia, and normal esophageal finding is correlated with glmM genotype. There was no association between age or sex of the patients and bacterial genotype.

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