Genomic circular RNA expression profile analysis indicated hsa_circRNA_000780 as a diagnostic marker for gastric cancer

Abstract Background: This study aimed to find the specific circular RNA (circRNA) for gastric cancer (GC) and lay the foundation for the early diagnosis of GC.Methods: Four patients with GC were selected for this study. The total RNA of their cancer tissues and adjacent tissues was extracted, and the circRNA was screened. The top eight upregulated and downregulated circRNAs with statistical significance between GC and paired adjacent nontumorous tissues were identified using real-time fluorescent quantitative polymerase chain reaction (PCR). The expression level of circRNA was identified by quantitative reverse transcription PCR in 78 cases of GC and its adjacent tissues, and in the gastric juice of 30 patients with chronic nonatrophic gastritis, 30 with chronic atrophic gastritis, 21 with early GC, and 57 with advanced GC.Results: A total of 445 circRNAs, including 69 upregulated and 376 downregulated circRNAs, were found to be significantly aberrantly expressed in GC tissues. Most of the differentially expressed circRNAs originated from chr1, chr3, chr4, chr6, and chr11. Hsa_circRNA_000780 was significantly downregulated in 80.77% of GC tissues. Its level in GC tissues correlated with the tumor size, tumor stage, T stage, venous invasion, carcinoembryonic antigen, and carbohydrate antigen 19-9 expression. More importantly, hsa_circRNA_000780 was found in the gastric juice of early and advanced GC.Conclusions: This study uncovered the new hsa_circRNA expression profile in human GC. Among them, hsa_circRNA_000780 was significantly downregulated in GC tissues and gastric juice. It had the potential to be used as a novel biomarker for the screening of early GC.

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Circular RNA hsa_circ_000780 has been identified by genomic expression profile analysis as a potential diagnostic marker for gastric cancer

10.21203/rs.3.rs-20318/v2 ◽

2020 ◽

Author(s):

Jian Song ◽

Shuyong Yu ◽

Dunjing Zhong ◽

Weizhong Yang ◽

Zhen Jia ◽

...

Keyword(s):

Gastric Cancer ◽

Expression Profile ◽

Profile Analysis ◽

Quantitative Polymerase Chain Reaction ◽

Venous Invasion ◽

Tumor Stage ◽

Circular Rna ◽

Chronic Atrophic Gastritis ◽

Gastric Fluid ◽

Aberrant Expression

Abstract Background : The present study attempted to detect a specific circular RNA (circRNA) for the early diagnosis of gastric cancer (GC). Methods: We selected four patients with GC for this study. The total RNA of the malignant and adjacent tissues was extracted, and the circRNA was screened. The eight most upregulated and downregulated circRNAs with a statistically significant relation to GC and paired adjacent nontumorous tissues were identified using real-time fluorescent quantitative polymerase chain reaction (PCR). CircRNA expression was identified by quantitative reverse transcriptase PCR in 78 cases of GC and adjacent tissues, and in the gastric fluids of 30 patients with chronic nonatrophic gastritis, 30 patients with chronic atrophic gastritis, 21 with early GC, and 57 with advanced GC. Results: A total of 445 circRNAs, including 69 upregulated and 376 downregulated circRNAs, showed significant aberrant expression in GC tissues. A majority of the differentially expressed circRNAs originated from chr1, chr3, chr4, chr6, and chr11. Hsa_circ_000780 was significantly downregulated in 80.77% of GC tissues, and its level in GC tissues correlated with the tumor size, tumor stage, T stage, venous invasion, carcinoembryonic antigen, and carbohydrate antigen 19-9 expression. A crucial observation was the presence of this circRNA in the gastric fluid of patients with early and advanced GC. Conclusions: The present study uncovered a new hsa_circRNA expression profile in human GC, of which hsa_circ_000780 was significantly downregulated in GC tissues and in gastric fluid. It can be potentially used as a novel biomarker for early GC screening.

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The Circular RNA hsa_circ_000780 as a Potential Molecular Diagnostic Target for Gastric Cancer

10.21203/rs.3.rs-257988/v1 ◽

2021 ◽

Author(s):

Jian Song ◽

Shuyong Yu ◽

Dunjing Zhong ◽

Weizhong Yang ◽

Zhen Jia ◽

...

Keyword(s):

Gastric Cancer ◽

Atrophic Gastritis ◽

Quantitative Polymerase Chain Reaction ◽

Circular Rna ◽

Chronic Atrophic Gastritis ◽

Gastric Fluid ◽

Molecular Diagnostic ◽

Tissue Samples ◽

Altered Expression ◽

Adjacent Tissue

Abstract Background: The present study aimed to identify a specific circular RNA (circRNA) for early diagnosis of gastric cancer (GC).Methods: Totally 82 patients with GC, 30 with chronic nonatrophic gastritis and 30 with chronic atrophic gastritis were included in this study. Four of the 82 GC patients were selected for screening. Total RNA from malignant and adjacent tissue samples was extracted, and circRNAs in four patients were screened. According to the screening results, the eight most upregulated and downregulated circRNAs with a statistically significant association with GC were identified by real-time fluorescent quantitative polymerase chain reaction (PCR). Then, the most regulated circRNA was selected for further sensitivity and specificity assessments. CircRNA expression was examined by quantitative reverse transcriptase PCR in 78 GC and adjacent tissue samples, as well as in gastric fluid samples from 30 patients with chronic nonatrophic gastritis, 30 with chronic atrophic gastritis, 21 with early GC, and 57 with advanced GC.Results: A total of 445 circRNAs, including 69 upregulated and 376 downregulated circRNAs, showed significantly altered expression in GC tissue samples. Hsa_circ_000780 was significantly downregulated in 80.77% of GC tissue samples, with levels in GC tissue samples correlating with tumor size, tumor stage, T stage, venous invasion, carcinoembryonic antigen amounts, and carbohydrate antigen 19-9 levels. Strikingly, this circRNA was found in the gastric fluid of patients with early and advanced GC.Conclusions: The present study uncovered a new circRNA expression profile in human GC, with hsa_circ_000780 significantly downregulated in GC tissue and gastric fluid specimens. These findings indicate that hsa_circ_000780 should be considered a novel biomarker for early GC screening.

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The circular RNA hsa_circ_000780 as a potential molecular diagnostic target for gastric cancer

BMC Medical Genomics ◽

10.1186/s12920-021-01096-6 ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Jian Song ◽

Shuyong Yu ◽

Dunjing Zhong ◽

Weizhong Yang ◽

Zhen Jia ◽

...

Keyword(s):

Gastric Cancer ◽

Atrophic Gastritis ◽

Quantitative Polymerase Chain Reaction ◽

Circular Rna ◽

Chronic Atrophic Gastritis ◽

Gastric Fluid ◽

Molecular Diagnostic ◽

Tissue Samples ◽

Altered Expression ◽

Adjacent Tissue

Abstract Background The present study aimed to identify a specific circular RNA (circRNA) for early diagnosis of gastric cancer (GC). Methods Totally 82 patients with GC, 30 with chronic nonatrophic gastritis and 30 with chronic atrophic gastritis were included in this study. Four of the 82 GC patients were selected for screening. Total RNA from malignant and adjacent tissue samples was extracted, and circRNAs in four patients were screened. According to the screening results, the eight most upregulated and downregulated circRNAs with a statistically significant association with GC were identified by real-time fluorescent quantitative polymerase chain reaction (PCR). Then, the most regulated circRNA was selected for further sensitivity and specificity assessments. CircRNA expression was examined by quantitative reverse transcriptase PCR in 78 GC (21 and 57 early and advanced GC, respectively) and adjacent tissue samples, as well as in gastric fluid samples from 30 patients with chronic nonatrophic gastritis, 30 with chronic atrophic gastritis, and 78 GC. Results A total of 445 circRNAs, including 69 upregulated and 376 downregulated circRNAs, showed significantly altered expression in GC tissue samples. Hsa_circ_000780 was significantly downregulated in 80.77% of GC tissue samples, with levels in GC tissue samples correlating with tumor size, tumor stage, T stage, venous invasion, carcinoembryonic antigen amounts, and carbohydrate antigen 19–9 levels. Strikingly, this circRNA was found in the gastric fluid of patients with early and advanced GC. Conclusions The present study uncovered a new circRNA expression profile in human GC, with hsa_circ_000780 significantly downregulated in GC tissue and gastric fluid specimens. These findings indicate that hsa_circ_000780 should be considered a novel biomarker for early GC screening.

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The Diagnosis Value of Promoter Methylation of UCHL1 in the Serum for Progression of Gastric Cancer

BioMed Research International ◽

10.1155/2015/741030 ◽

2015 ◽

Vol 2015 ◽

pp. 1-8 ◽

Cited By ~ 5

Author(s):

Gongping Wang ◽

Wei Zhang ◽

Bo Zhou ◽

Canhui Jin ◽

Zengfang Wang ◽

...

Keyword(s):

Gastric Cancer ◽

Promoter Methylation ◽

Poor Prognosis ◽

Methylation Status ◽

Tumor Stage ◽

Chronic Atrophic Gastritis ◽

Dependent Manner ◽

Specific Pcr ◽

And Control

Background.Aberrant promoter methylation has been considered as a potential molecular marker for gastric cancer (GC). However, the role of methylation of FLNC, THBS1, and UCHL1 in the development and progression of GC has not been explored.Methods.The promoter methylation status of UCHL1, FLNC, THBS1, and DLEC1 was assessed by quantitative methylation-specific PCR (QMSP) in the serum of 82 GC patients, 46 chronic atrophic gastritis (CAG) subjects, and 40 healthy controls.Results.All four genes had significantly higher methylation levels in GC patients than in CAG and control subjects. However, only UCHL1 methylation was significantly correlated with the tumor stage and lymph node metastasis. While THBS1 methylation was altered in an age-dependent manner, FLNC methylation was correlated with differentiation andHelicobacter pyloriinfection. DLEC1 methylation was only associated with tumor size. Moreover, methylated UCHL1 with or without THBS1 in the serum was found to be significantly associated with a poor prognosis.Conclusion.The promoter methylation degree of FLNC, THBS1, UCHL1, and DLEC1 in serum could tell the existence of GC and only UCHL1 in the serum was also associated with poor prognosis of GC.

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Negative Correlation Between Circular RNA SMARC5 and MicroRNA 432, and Their Clinical Implications in Bladder Cancer Patients

Technology in Cancer Research & Treatment ◽

10.1177/15330338211039110 ◽

2021 ◽

Vol 20 ◽

pp. 153303382110391

Author(s):

Zhijia Zhang ◽

Yanxia Sang ◽

Zhengan Liu ◽

Jinkai Shao

Keyword(s):

Bladder Cancer ◽

Cancer Patients ◽

Survival Data ◽

Tumor Size ◽

Quantitative Polymerase Chain Reaction ◽

Disease Free Survival ◽

Tumor Stage ◽

Circular Rna ◽

High Expression ◽

Tumor Tissues

Objective: Our study aimed to evaluate the correlation of circular RNA SMARCA5 (circ-SMARCA5) and microRNA 432 (miR-432) with clinical characteristics and survival in bladder cancer patients. Methods: Preoperative clinicopathologic features and survival data of 156 bladder cancer patients were retrospectively reviewed. A total of 156 cases of tumor tissues, whereas 71 cases out of 156 available adjacent tissues were obtained from the Pathology Department for circ-SMARCA5 and miR-432 detections using real-time quantitative polymerase chain reaction. Results: Circ-SMARCA5 was upregulated but miR-432 was downregulated in tumor tissues compared with adjacent tissues; meanwhile, circ-SMARCA5 expression was negatively correlated with miR-432 in bladder cancer tissues. Circ-SMARCA5 high expression was correlated with larger tumor size, higher tumor stage, and lymph node (LYN) metastasis. However, miR-432 high expression was correlated with single multiplicity, smaller tumor size, lower tumor stage, less LYN metastasis in bladder cancer patients. Regarding survival, circ-SMARCA5 high expression was correlated with shorter disease-free survival (DFS) and overall survival (OS); whereas, miR-432 high expression was correlated with longer DFS and OS in bladder cancer patients. Further multivariate Cox's regression analysis displayed that circ-SMARCA5 high expression was an independent predictive factor for both worse DFS and OS in bladder cancer patients. Conclusion: Circ-SMARCA5 high expression but miR-432 low expression is correlated with advanced tumor features and poor survival of bladder cancer patients, which present as potential prognostic markers in bladder cancer.

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CEA Levels in Gastric Juice in Precancerous Conditions and Cancer

The International Journal of Biological Markers ◽

10.1177/172460088700200208 ◽

1987 ◽

Vol 2 (2) ◽

pp. 101-104 ◽

Cited By ~ 2

Author(s):

Dino Amador ◽

Alberto Ravaioli ◽

Roberta Biserni ◽

Chiara Bonaguri ◽

Paola Erbacci ◽

...

Keyword(s):

Gastric Cancer ◽

Gastric Carcinoma ◽

Gastric Juice ◽

Specific Antigen ◽

Chronic Atrophic Gastritis ◽

Precancerous Conditions ◽

Risk Indicator ◽

Gastric Lesions ◽

Clinical Routine ◽

Malignant Neoplasia

First described in 1965 as a specific antigen for cancer of the colon, CEA is now considered to be an antigen associated with many types of malignant neoplasia, although the CEA-Test's role in clinical routine has yet to be clearly defined. In the present study CEA levels in gastric juice were measured in subjects with gastric carcinoma (n = 25) and with benign gastric lesions (n = 171). CEA was significantly (p < 0.05) higher in patients with gastric carcinoma (GC) than in subjects with benign gastric lesions, other than chronic atrophic gastritis (CAG) associated with intestinal metaplasia (IM). In this latter condition CEA levels were similar to those in patients with GC. These results suggest that the assay of CEA in gastric juice could be included in the diagnostic program for gastric cancer and its precursors with the aim of assessing its utility as risk indicator in the management of precancerous conditions and lesion.

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