scholarly journals Circular RNA hsa_circ_000780 has been identified by genomic expression profile analysis as a potential diagnostic marker for gastric cancer

2020 ◽  
Author(s):  
Jian Song ◽  
Shuyong Yu ◽  
Dunjing Zhong ◽  
Weizhong Yang ◽  
Zhen Jia ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Expression Profile ◽  
Profile Analysis ◽  
Quantitative Polymerase Chain Reaction ◽  
Venous Invasion ◽  
Tumor Stage ◽  
Circular Rna ◽  
Chronic Atrophic Gastritis ◽  
Gastric Fluid ◽  
Aberrant Expression

Abstract Background : The present study attempted to detect a specific circular RNA (circRNA) for the early diagnosis of gastric cancer (GC). Methods: We selected four patients with GC for this study. The total RNA of the malignant and adjacent tissues was extracted, and the circRNA was screened. The eight most upregulated and downregulated circRNAs with a statistically significant relation to GC and paired adjacent nontumorous tissues were identified using real-time fluorescent quantitative polymerase chain reaction (PCR). CircRNA expression was identified by quantitative reverse transcriptase PCR in 78 cases of GC and adjacent tissues, and in the gastric fluids of 30 patients with chronic nonatrophic gastritis, 30 patients with chronic atrophic gastritis, 21 with early GC, and 57 with advanced GC. Results: A total of 445 circRNAs, including 69 upregulated and 376 downregulated circRNAs, showed significant aberrant expression in GC tissues. A majority of the differentially expressed circRNAs originated from chr1, chr3, chr4, chr6, and chr11. Hsa_circ_000780 was significantly downregulated in 80.77% of GC tissues, and its level in GC tissues correlated with the tumor size, tumor stage, T stage, venous invasion, carcinoembryonic antigen, and carbohydrate antigen 19-9 expression. A crucial observation was the presence of this circRNA in the gastric fluid of patients with early and advanced GC. Conclusions: The present study uncovered a new hsa_circRNA expression profile in human GC, of which hsa_circ_000780 was significantly downregulated in GC tissues and in gastric fluid. It can be potentially used as a novel biomarker for early GC screening.

scholarly journals Genomic circular RNA expression profile analysis indicated hsa_circRNA_000780 as a diagnostic marker for gastric cancer

2020 ◽  
Author(s):  
Jian Song ◽  
Shuyong Yu ◽  
Dunjing Zhong ◽  
Weizhong Yang ◽  
Zhen Jia ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Gastric Juice ◽  
Expression Profile ◽  
Profile Analysis ◽  
Quantitative Polymerase Chain Reaction ◽  
Statistical Significance ◽  
Venous Invasion ◽  
Tumor Stage ◽  
Circular Rna ◽  
Chronic Atrophic Gastritis

Abstract Background: This study aimed to find the specific circular RNA (circRNA) for gastric cancer (GC) and lay the foundation for the early diagnosis of GC.Methods: Four patients with GC were selected for this study. The total RNA of their cancer tissues and adjacent tissues was extracted, and the circRNA was screened. The top eight upregulated and downregulated circRNAs with statistical significance between GC and paired adjacent nontumorous tissues were identified using real-time fluorescent quantitative polymerase chain reaction (PCR). The expression level of circRNA was identified by quantitative reverse transcription PCR in 78 cases of GC and its adjacent tissues, and in the gastric juice of 30 patients with chronic nonatrophic gastritis, 30 with chronic atrophic gastritis, 21 with early GC, and 57 with advanced GC.Results: A total of 445 circRNAs, including 69 upregulated and 376 downregulated circRNAs, were found to be significantly aberrantly expressed in GC tissues. Most of the differentially expressed circRNAs originated from chr1, chr3, chr4, chr6, and chr11. Hsa_circRNA_000780 was significantly downregulated in 80.77% of GC tissues. Its level in GC tissues correlated with the tumor size, tumor stage, T stage, venous invasion, carcinoembryonic antigen, and carbohydrate antigen 19-9 expression. More importantly, hsa_circRNA_000780 was found in the gastric juice of early and advanced GC.Conclusions: This study uncovered the new hsa_circRNA expression profile in human GC. Among them, hsa_circRNA_000780 was significantly downregulated in GC tissues and gastric juice. It had the potential to be used as a novel biomarker for the screening of early GC.

The Circular RNA hsa_circ_000780 as a Potential Molecular Diagnostic Target for Gastric Cancer

2021 ◽  
Author(s):  
Jian Song ◽  
Shuyong Yu ◽  
Dunjing Zhong ◽  
Weizhong Yang ◽  
Zhen Jia ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Atrophic Gastritis ◽  
Quantitative Polymerase Chain Reaction ◽  
Circular Rna ◽  
Chronic Atrophic Gastritis ◽  
Gastric Fluid ◽  
Molecular Diagnostic ◽  
Tissue Samples ◽  
Altered Expression ◽  
Adjacent Tissue

Abstract Background: The present study aimed to identify a specific circular RNA (circRNA) for early diagnosis of gastric cancer (GC).Methods: Totally 82 patients with GC, 30 with chronic nonatrophic gastritis and 30 with chronic atrophic gastritis were included in this study. Four of the 82 GC patients were selected for screening. Total RNA from malignant and adjacent tissue samples was extracted, and circRNAs in four patients were screened. According to the screening results, the eight most upregulated and downregulated circRNAs with a statistically significant association with GC were identified by real-time fluorescent quantitative polymerase chain reaction (PCR). Then, the most regulated circRNA was selected for further sensitivity and specificity assessments. CircRNA expression was examined by quantitative reverse transcriptase PCR in 78 GC and adjacent tissue samples, as well as in gastric fluid samples from 30 patients with chronic nonatrophic gastritis, 30 with chronic atrophic gastritis, 21 with early GC, and 57 with advanced GC.Results: A total of 445 circRNAs, including 69 upregulated and 376 downregulated circRNAs, showed significantly altered expression in GC tissue samples. Hsa_circ_000780 was significantly downregulated in 80.77% of GC tissue samples, with levels in GC tissue samples correlating with tumor size, tumor stage, T stage, venous invasion, carcinoembryonic antigen amounts, and carbohydrate antigen 19-9 levels. Strikingly, this circRNA was found in the gastric fluid of patients with early and advanced GC.Conclusions: The present study uncovered a new circRNA expression profile in human GC, with hsa_circ_000780 significantly downregulated in GC tissue and gastric fluid specimens. These findings indicate that hsa_circ_000780 should be considered a novel biomarker for early GC screening.

scholarly journals The circular RNA hsa_circ_000780 as a potential molecular diagnostic target for gastric cancer

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Jian Song ◽  
Shuyong Yu ◽  
Dunjing Zhong ◽  
Weizhong Yang ◽  
Zhen Jia ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Atrophic Gastritis ◽  
Quantitative Polymerase Chain Reaction ◽  
Circular Rna ◽  
Chronic Atrophic Gastritis ◽  
Gastric Fluid ◽  
Molecular Diagnostic ◽  
Tissue Samples ◽  
Altered Expression ◽  
Adjacent Tissue

Abstract Background The present study aimed to identify a specific circular RNA (circRNA) for early diagnosis of gastric cancer (GC). Methods Totally 82 patients with GC, 30 with chronic nonatrophic gastritis and 30 with chronic atrophic gastritis were included in this study. Four of the 82 GC patients were selected for screening. Total RNA from malignant and adjacent tissue samples was extracted, and circRNAs in four patients were screened. According to the screening results, the eight most upregulated and downregulated circRNAs with a statistically significant association with GC were identified by real-time fluorescent quantitative polymerase chain reaction (PCR). Then, the most regulated circRNA was selected for further sensitivity and specificity assessments. CircRNA expression was examined by quantitative reverse transcriptase PCR in 78 GC (21 and 57 early and advanced GC, respectively) and adjacent tissue samples, as well as in gastric fluid samples from 30 patients with chronic nonatrophic gastritis, 30 with chronic atrophic gastritis, and 78 GC. Results A total of 445 circRNAs, including 69 upregulated and 376 downregulated circRNAs, showed significantly altered expression in GC tissue samples. Hsa_circ_000780 was significantly downregulated in 80.77% of GC tissue samples, with levels in GC tissue samples correlating with tumor size, tumor stage, T stage, venous invasion, carcinoembryonic antigen amounts, and carbohydrate antigen 19–9 levels. Strikingly, this circRNA was found in the gastric fluid of patients with early and advanced GC. Conclusions The present study uncovered a new circRNA expression profile in human GC, with hsa_circ_000780 significantly downregulated in GC tissue and gastric fluid specimens. These findings indicate that hsa_circ_000780 should be considered a novel biomarker for early GC screening.

scholarly journals The Diagnosis Value of Promoter Methylation of UCHL1 in the Serum for Progression of Gastric Cancer

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Gongping Wang ◽  
Wei Zhang ◽  
Bo Zhou ◽  
Canhui Jin ◽  
Zengfang Wang ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Promoter Methylation ◽  
Poor Prognosis ◽  
Methylation Status ◽  
Tumor Stage ◽  
Chronic Atrophic Gastritis ◽  
Dependent Manner ◽  
Specific Pcr ◽  
And Control

Background.Aberrant promoter methylation has been considered as a potential molecular marker for gastric cancer (GC). However, the role of methylation of FLNC, THBS1, and UCHL1 in the development and progression of GC has not been explored.Methods.The promoter methylation status of UCHL1, FLNC, THBS1, and DLEC1 was assessed by quantitative methylation-specific PCR (QMSP) in the serum of 82 GC patients, 46 chronic atrophic gastritis (CAG) subjects, and 40 healthy controls.Results.All four genes had significantly higher methylation levels in GC patients than in CAG and control subjects. However, only UCHL1 methylation was significantly correlated with the tumor stage and lymph node metastasis. While THBS1 methylation was altered in an age-dependent manner, FLNC methylation was correlated with differentiation andHelicobacter pyloriinfection. DLEC1 methylation was only associated with tumor size. Moreover, methylated UCHL1 with or without THBS1 in the serum was found to be significantly associated with a poor prognosis.Conclusion.The promoter methylation degree of FLNC, THBS1, UCHL1, and DLEC1 in serum could tell the existence of GC and only UCHL1 in the serum was also associated with poor prognosis of GC.

scholarly journals Negative Correlation Between Circular RNA SMARC5 and MicroRNA 432, and Their Clinical Implications in Bladder Cancer Patients

2021 ◽  
Vol 20 ◽  
pp. 153303382110391
Author(s):  
Zhijia Zhang ◽  
Yanxia Sang ◽  
Zhengan Liu ◽  
Jinkai Shao
Keyword(s):  
Bladder Cancer ◽  
Cancer Patients ◽  
Survival Data ◽  
Tumor Size ◽  
Quantitative Polymerase Chain Reaction ◽  
Disease Free Survival ◽  
Tumor Stage ◽  
Circular Rna ◽  
High Expression ◽  
Tumor Tissues

Objective: Our study aimed to evaluate the correlation of circular RNA SMARCA5 (circ-SMARCA5) and microRNA 432 (miR-432) with clinical characteristics and survival in bladder cancer patients. Methods: Preoperative clinicopathologic features and survival data of 156 bladder cancer patients were retrospectively reviewed. A total of 156 cases of tumor tissues, whereas 71 cases out of 156 available adjacent tissues were obtained from the Pathology Department for circ-SMARCA5 and miR-432 detections using real-time quantitative polymerase chain reaction. Results: Circ-SMARCA5 was upregulated but miR-432 was downregulated in tumor tissues compared with adjacent tissues; meanwhile, circ-SMARCA5 expression was negatively correlated with miR-432 in bladder cancer tissues. Circ-SMARCA5 high expression was correlated with larger tumor size, higher tumor stage, and lymph node (LYN) metastasis. However, miR-432 high expression was correlated with single multiplicity, smaller tumor size, lower tumor stage, less LYN metastasis in bladder cancer patients. Regarding survival, circ-SMARCA5 high expression was correlated with shorter disease-free survival (DFS) and overall survival (OS); whereas, miR-432 high expression was correlated with longer DFS and OS in bladder cancer patients. Further multivariate Cox's regression analysis displayed that circ-SMARCA5 high expression was an independent predictive factor for both worse DFS and OS in bladder cancer patients. Conclusion: Circ-SMARCA5 high expression but miR-432 low expression is correlated with advanced tumor features and poor survival of bladder cancer patients, which present as potential prognostic markers in bladder cancer.

scholarly journals Global circular RNA expression profile of human gastric cancer and its clinical significance

2017 ◽  
Vol 6 (6) ◽  
pp. 1173-1180 ◽  
Author(s):  
Yongfu Shao ◽  
Jinyun Li ◽  
Rongdan Lu ◽  
Tianwen Li ◽  
Yunben Yang ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Clinical Significance ◽  
Expression Profile ◽  
Circular Rna ◽  
Rna Expression ◽  
Human Gastric Cancer

scholarly journals Expression profile analysis to identify circular RNA expression signatures in hair follicle of Hu sheep lambskin

Genomics ◽  
2020 ◽  
Vol 112 (6) ◽  
pp. 4454-4462
Author(s):  
Xiaoyang Lv ◽  
Weihao Chen ◽  
Wei Sun ◽  
Zahid Hussain ◽  
Ling Chen ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Expression Profile ◽  
Profile Analysis ◽  
Circular Rna ◽  
Rna Expression ◽  
Expression Profile Analysis ◽  
Hu Sheep

scholarly journals TRPV2 Calcium Channel Gene Expression and Outcomes in Gastric Cancer Patients: A Clinically Relevant Association

2019 ◽  
Vol 8 (5) ◽  
pp. 662 ◽  
Author(s):  
Pietro Zoppoli ◽  
Giovanni Calice ◽  
Simona Laurino ◽  
Vitalba Ruggieri ◽  
Francesco La Rocca ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gastric Cancer ◽  
Intracellular Calcium ◽  
Tissue Microarrays ◽  
Causative Factor ◽  
Tumor Stage ◽  
Rank Test ◽  
Apoptosis Resistance ◽  
Aberrant Expression ◽  
Normal Stomach

Gastric cancer (GC) is characterized by poor efficacy and the modest clinical impact of current therapies. Apoptosis evasion represents a causative factor for treatment failure in GC as in other cancers. Since intracellular calcium homeostasis regulation has been found to be associated with apoptosis resistance, the aberrant expression of intracellular calcium regulator genes (CaRGs) could have a prognostic value in GC patients. We analyzed the association of the expression levels of 98 CaRGs with prognosis by the log-rank test in a collection of 1524 GC samples from four gene expression profiling datasets. We also evaluated differential gene expression in comparison with normal stomach tissue, and then we crossed results with tissue microarrays from the Human Protein Atlas. Among the investigated CaRGs, patients with high levels of TRPV2 expression were characterized by a shorter overall survival. TRPV2 expression was found to increase according to tumor stage. Both mRNA and protein levels were significantly higher in tumor than normal stomach samples. TRPV2 was also associated with poor prognosis in the Lauren’s intestinal type GC and in patients treated with adjuvant therapy. Overall, we highlighted the relevance of TRPV2 not only as a prognostic biomarker but also as a potential therapeutic target to improve GC treatment efficacy.

scholarly journals Circular RNA hsa_circ_0044234 as distinct molecular signature of triple negative breast cancer: a potential regulator of GATA3

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Farzaneh Darbeheshti ◽  
Elham Zokaei ◽  
Yaser Mansoori ◽  
Sima Emadi Allahyari ◽  
Zeeba Kamaliyan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Lines ◽  
Triple Negative Breast Cancer ◽  
Triple Negative ◽  
Profile Analysis ◽  
Quantitative Polymerase Chain Reaction ◽  
Circular Rna ◽  
Molecular Signature ◽  
Circular Rnas ◽  
Roc Curve Analysis

Abstract Background Circular RNAs (circRNAs) have been implicated in the initiation and development of breast cancer as functional non-coding RNAs (ncRNA). The roles of circRNAs as the competing endogenous RNAs (ceRNAs) to sponge microRNAs (miRNAs) have also been indicated. However, the functions of circRNAs in breast cancer have not been totally elucidated. This study aimed to explore the clinical implications and possible roles of circ_0044234 in carcinogenesis of the most problematic BC subtype, triple negative breast cancer (TNBC), which are in desperate need of biomarkers and targeted therapies. Methods The importance of circ_0044234 as one of the most dysregulated circRNAs in TNBC was discovered through microarray expression profile analysis. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to confirm the downregulation of circ_0044234 in triple negative tumors and cell lines versus non-triple negative ones. The bioinformatics prediction revealed that circ_0044234 could act as an upstream sponge in the miR-135b/GATA3 axis, two of the most dysregulated transcripts in TNBC. Results Our experimental investigation of circ_0044234 expressions in various BC subtypes as well as cell lines reveals that TNBC expresses circ_0044234 at a substantially lower level than non-TNBC. The ROC curve analysis indicates that it could be applied as a discriminative biomarker to identify TNBC from other BC subtypes. Moreover, circ_0044234 expression could be an independent prognostic biomarker in BC. Interestingly, a substantial inverse expression correlation was detected between circ_0044234 and miR-135b-5p as well as between miR-135b-5p and GATA3 in breast tumors. Conclusions The possible clinical usefulness of circ_0044234 as a promising distinct biomarker and upcoming therapeutic target for TNBC have been indicated in this research. Our comprehensive approach revealed the potential circ_0044234/miR135b-5p/GATA3 ceRNA axis in TNBC.

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