scholarly journals Heterologous Expression of Cyanobacterial PCS Confers Augmented Arsenic And Cadmium Stress Tolerance and Higher Artemisinin In Artemisia Annua Hairy Roots.

2021 ◽  
Author(s):  
Neha Pandey ◽  
Krishna Kumar Rai ◽  
Sanjay Kumar Rai ◽  
Shashi Pandey-Rai
Keyword(s):  
Heavy Metals ◽  
Heterologous Expression ◽  
Hairy Roots ◽  
Artemisia Annua ◽  
In Silico Analysis ◽  
Cadmium Stress ◽  
Functional Domain ◽  
Cd Accumulation ◽  
Transformed Hairy Roots ◽  
Pcc 7120

Abstract The present study provides the first report of heterologous expression of phytochelatin synthase from Anabaena PCC 7120 (anaPCS) into the hairy roots of Artemisia annua. Transformed hairy roots of A. annua expressing anaPCS gene showed better tolerance to heavy metals viz., arsenic (As) and cadmium (Cd) owing to 143 and 191 % more As and Cd accumulation respectively as compared to normal roots with a bioconcentration factor (BCF) of 9.7 and 21.1 for As and Cd respectively. Under As and Cd stresses, transformed hairy roots possessed significantly higher amounts of phytochelatins and thiols probably due to the presence of both AaPCS (Artemisia annua PCS) and anaPCS. In addition, artemisinin synthesis was also induced in transformed hairy roots under heavy metals stresses. In-silico analysis revealed the presence of conserved motifs in both AaPCS and anaPCS sequences as well as structural modelling of PCS functional domain was conducted. Interaction of AaPCS and anaPCS proteins with CdCl2 and sodium arsenate gene ontology analysis gave insights to anaPCS functioning in transformed hairy roots of A. annua. The study provides transformed hairy roots of A. annua as an efficient tool for effective phytoremediation with added advantages of artemisinin extraction from hairy roots used for phytoremediation.

scholarly journals Efficacy of Indole Acetic Acid and Exopolysaccharides-Producing Bacillus safensis Strain FN13 for Inducing Cd-Stress Tolerance and Plant Growth Promotion in Brassica juncea (L.)

2021 ◽  
Vol 11 (9) ◽  
pp. 4160
Author(s):  
Farheen Nazli ◽  
Xiukang Wang ◽  
Maqshoof Ahmad ◽  
Azhar Hussain ◽  
Bushra ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Plant Growth ◽  
Stress Tolerance ◽  
Brassica Juncea ◽  
Contaminated Soils ◽  
Effective Strain ◽  
Antioxidant Enzyme Activities ◽  
Cd Stress ◽  
Cd Accumulation ◽  
Bacillus Safensis

Untreated wastewater used for irrigating crops is the major source of toxic heavy metals and other pollutants in soils. These heavy metals affect plant growth and deteriorate the quality of edible parts of growing plants. Phytohormone (IAA) and exopolysaccharides (EPS) producing plant growth-promoting rhizobacteria can reduce the toxicity of metals by stabilizing them in soil. The present experiment was conducted to evaluate the IAA and EPS-producing rhizobacterial strains for improving growth, physiology, and antioxidant activity of Brassica juncea (L.) under Cd-stress. Results showed that Cd-stress significantly decreased the growth and physiological parameters of mustard plants. Inoculation with Cd-tolerant, IAA and EPS-producing rhizobacterial strains, however, significantly retrieved the inhibitory effects of Cd-stress on mustard growth, and physiology by up regulating antioxidant enzyme activities. Higher Cd accumulation and proline content was observed in the roots and shoot tissues upon Cd-stress in mustard plants while reduced proline and Cd accumulation was recorded upon rhizobacterial strains inoculation. Maximum decrease in proline contents (12.4%) and Cd concentration in root (26.9%) and shoot (29%) in comparison to control plants was observed due to inoculation with Bacillus safensis strain FN13. The activity of antioxidant enzymes was increased due to Cd-stress; however, the inoculation with Cd-tolerant, IAA-producing rhizobacterial strains showed a non-significant impact in the case of the activity of superoxide dismutase (SOD), peroxidase (POX) and catalase (CAT) in Brassica juncea (L.) plants under Cd-stress. Overall, Bacillus safensis strain FN13 was the most effective strain in improving the Brassica juncea (L.) growth and physiology under Cd-stress. It can be concluded, as the strain FN13 is a potential phytostabilizing biofertilizer for heavy metal contaminated soils, that it can be recommended to induce Cd-stress tolerance in crop plants.

scholarly journals The Influence of Zn and Cd Accumulation on the Growth and Development of Medicinal Plants in the Impact Zone of the Novocherkassk Power Station

2022 ◽  
Author(s):  
Victor Chaplygin ◽  
Tatiana Minkina ◽  
Saglara Mandzhieva ◽  
Dina Nevidomskaya ◽  
Natalia Chernikova ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Chemical Composition ◽  
Poa Pratensis ◽  
Negative Consequences ◽  
Industrial Emissions ◽  
Power Station ◽  
Cd Accumulation ◽  
Elytrigia Repens ◽  
Medical Plants ◽  
The Impact

Over the pastdecade, particular attention has been paid to studies of the chemical composition of medical plants to identify the possible negative consequences of using raw plant material polluted with heavy metals for the production of medical drugs. In our study, we analyzed the chemical composition of the medical plants growing in the impact area of the Novocherkassk power station. Specifically, the plants Artemisia austriaca, Poa pratensis and Elytrigia repenswere examined for the analysis.The content and distribution of Zn and Cd, which are most distributed in industrial emissions and belong to the first class of hazardous elements, were measured. The maximum permissible content (MPC) of Zn in the raw material of Artemisia austriaca and Elytrigia repens was found, as was the maximum content of Cd in all analyzed plants growing in the 5km area around thepower station. The plant Artemisia austriacawasfound to have Zn and Cd accumulation in itsabovegroundcomponents, while in Poa pratensis and Elytrigia repens, accumulation was in the roots. The morphobiometric parameters of the plants were mostly dependent on the soil properties, followed by the degree of technogenic load. The content of Zn and Cd in the medical drugs was higher than the MPC without visible features of heavy metal pollution and so these plants weredangerous for human health. Keywords: heavy metals, technogenic load, phytoreagents, morphometric parameters

scholarly journals Conservation of the Prion Properties of Ure2p through Evolution

2003 ◽  
Vol 14 (8) ◽  
pp. 3449-3458 ◽  
Author(s):  
Agnès Baudin-Baillieu ◽  
Eric Fernandez-Bellot ◽  
Fabienne Reine ◽  
Eric Coissac ◽  
Christophe Cullin
Keyword(s):  
Functional Analysis ◽  
Candida Albicans ◽  
Gene Deletion ◽  
Yeast Species ◽  
Kluyveromyces Lactis ◽  
In Silico Analysis ◽  
Functional Domain ◽  
Extreme Situation ◽  
Homologous Genes ◽  
Silico Analysis

The yeast inheritable [URE3] element corresponds to a prion form of the nitrogen catabolism regulator Ure2p. We have isolated several orthologous URE2 genes in different yeast species: Saccharomyces paradoxus, S. uvarum, Kluyveromyces lactis, Candida albicans, and Schizosaccharomyces pombe. We show here by in silico analysis that the GST-like functional domain and the prion domain of the Ure2 proteins have diverged separately, the functional domain being more conserved through the evolution. The more extreme situation is found in the two S. pombe genes, in which the prion domain is absent. The functional analysis demonstrates that all the homologous genes except for the two S. pombe genes are able to complement the URE2 gene deletion in a S. cerevisiae strain. We show that in the two most closely related yeast species to S. cerevisiae, i.e., S. paradoxus and S. uvarum, the prion domains of the proteins have retained the capability to induce [URE3] in a S. cerevisiae strain. However, only the S. uvarum full-length Ure2p is able to behave as a prion. We also show that the prion inactivation mechanisms can be cross-transmitted between the S. cerevisiae and S. uvarum prions.

scholarly journals Cadmium stress in Dongying wild soybean seedlings: growth, Cd accumulation, and photosynthesis

2018 ◽  
Vol 56 (4) ◽  
pp. 1346-1352 ◽  
Author(s):  
L. Liu ◽  
Y. K. Shang ◽  
L. Li ◽  
Y. H. Chen ◽  
Z. Z. Qin ◽  
...  
Keyword(s):  
Wild Soybean ◽  
Cadmium Stress ◽  
Cd Accumulation ◽  
Soybean Seedlings
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