New laboratory criterion for the differential diagnosis of sarcoidosis and pulmonary tuberculosis

Abstract Take-Home Message.This study provides a new diagnostic index based on the antibodies to mutated citrullinated vimentin concentration and the level of different B subsets. Determination of index over 5 Units proved to be characteristic of sarcoidosis, what might help to differentiate SD from TB.Abstract. In some cases there is a problem of differential diagnosis of sarcoidosis(SD) and tuberculosis(TB)because of the similarities in clinical, X-ray and laboratory features. The aim of this study was to search for new differential diagnostic criteria for sarcoidosis and tuberculosis by calculating the index, based on the level of autoantibodies to modified citrullinated vimentin (anti-MCV) and the level of B-cell subpopulations. These parameters were measured in patients with sarcoidosis (n = 93), tuberculosis (n = 40) and healthy donors (n = 40) using the ELISA and cytometry. The absence of a statistically significant difference when comparing the level of anti-MCV, the number of B-cells in SD and TB suggests that these changes may be characteristic of granulomatous diseases. The use of the formula Ds=([B-naïve%]\[B-memory%])*([B-CD38%]+[B-CD5%])/[anti-MCV] might allow to differentiate SD with an increase in the calculated index of more than 5 units with a sensitivity of 80.00% and specificity of 93.10% (AUC = 0.926).

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Efficiency and safety of transbronchial cryobiopsy in the diagnosis of disseminated lung diseases in patients after unsuccessful transbronchial forceps lung biopsy

MedAlliance ◽

10.36422/23076348-2020-8-3-66-72 ◽

2020 ◽

Vol 8 (3) ◽

pp. 66-72

Keyword(s):

Differential Diagnosis ◽

Pulmonary Tuberculosis ◽

Lung Biopsy ◽

Lung Diseases ◽

Pleural Cavity ◽

Pulmonary Hemorrhage ◽

Transbronchial Biopsy ◽

Small Airways ◽

Safe Procedure ◽

X Ray

Disseminated lung diseases is a group of pathologies characterized by damage to the small airways, alveo-li, interstitium of the lungs united by a common symp-tom, dissemination in the lung tissue detected by X-ray. Pathologies included in the DLD group are numerous, and differential diagnosis between them is difficult, due to the similarity of symptoms and the absence of patho-gnomonic signs. Transbronchial cryobiopsy of the lungs is a relatively new technique, currently used in diagnos-tics, along with other methods of morphological verifi-cation in disseminated lung diseases. However, there are only sporadic publications on its use in Russia. The article describes the first experience of using transbronchial cry-obiopsy in patients with disseminated lung diseases after negative transbronchial biopsy. Objective. To obtain the first cli nical experience with the TBLC method. To assess the role and efficacy of TBLC in the diagnosis of DLDs. Ma-terials and methods. Patients with oncology in medical history or with previous tuberculosis were not included into the study; a total of 15 patients were included. The process was verified in 11 (73.33%) patients. Of these, sarcoidosis of the lungs was detected in 8 (72.73%) cases, pulmonary tuberculosis in 2 (18.18%), idiopathic intersti-tial pneumonia in 1 (9.09%). Complications were record-ed in 2 patients (13.33%): apical pneumothorax, which did not require pleural cavity drainage; and pulmonary hemorrhage requiring prolonged mechanical ventilation.Conclusions. Transbronchial cryobiopsy in patients with disseminated lung disease after unsuccessful transbron-chial lung biopsy appears to be a highly informative and relatively safe procedure. Cryobiopsy through a rigid bronchoscope tube appears to be safer. TBLC from the apex of the lungs, as well as in smokers, seems to be a more difficult task to verify, but this requires further studies

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Comparison of Chest X-Ray Findings Between Primary and Secondary Multidrug Resistant Pulmonary Tuberculosis

BioScientia Medicina ◽

10.32539/bsm.v5i10.356 ◽

2021 ◽

Vol 5 (10) ◽

pp. 903-910

Author(s):

Ricky Septafianty ◽

Anita Widyoningroem ◽

M. Yamin S. S ◽

Rosy Setiawati ◽

Soedarsono

Keyword(s):

Pulmonary Tuberculosis ◽

Multidrug Resistant ◽

X Rays ◽

Pulmonary Tb ◽

X Ray ◽

Pleural Thickening ◽

Significant Difference ◽

Record Data ◽

Mdr Tb ◽

Chest X Ray

Introduction: Radiological imaging has a key role in multidrug-resistant (MDR) pulmonary tuberculosis (TB) screening and diagnosis. However, new cases of MDR pulmonary TB are often overlooked; therefore, its transmission might continue before its diagnosis. The most widely used and affordable radiological modality is a chest radiograph. This study aims to describe the characteristics of primary and secondary MDR pulmonary TB chest x-ray findings for differential diagnosis. Methods: This study was an analytic observational study with a retrospective design. Researchers evaluated medical record data of primary and secondary MDR pulmonary TB patients who underwent chest x-ray examinations. The patient's chest x-rays were then evaluated. Evaluated variables were lung, pleural, and mediastinal abnormalities and severity category. Results: The most common chest x-ray finding in primary MDR pulmonary TB was consolidation (96.2%), which was mostly unilateral (52.0%), accompanied by cavities (71.2%), most of which were multiple (83.8%) with a moderate category of severity. The most common chest x-ray finding in secondary MDR pulmonary TB was consolidation (100%), which was mostly bilateral (60.4%), accompanied by cavities (80.2%), most of which were multiple (90.1%) with severe category of severity. Pleural thickening (47.5%) was also found. Conclusion: There was a significant difference between primary and secondary MDR pulmonary TB in terms of mild severity category, and pleural thickening. Mild severity category is mostly found in primary MDR-TB and pleural thickening is mostly found in secondary TB.

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The efficacy of different extrapleural fixations in upper-posterior thoracoplasty in fibrous cavernous pulmonary tuberculosis patients

Tuberculosis and lung diseases ◽

10.21292/2075-1230-2020-98-12-42-46 ◽

2021 ◽

Vol 98 (12) ◽

pp. 42-46

Author(s):

S. A. Belov ◽

A. A. Grigoryuk

Keyword(s):

Pulmonary Tuberculosis ◽

Polypropylene Mesh ◽

Tuberculosis Patients ◽

X Ray ◽

Significant Difference

The objective of the study: to compare the efficacy of different modifications of thoracoplasty in patients with apical fibrous cavernous pulmonary tuberculosis.Subjects and methods. The article describes the analysis of the results of thoracoplasty in 65 fibrous cavernous pulmonary tuberculosis patients performed from 2016 to 2019. Three types of thoracoplasty were used: thoracoplasty with polypropylene mesh, osteoplastic thoracoplasty, and traditional thoracoplasty. The results were assessed by the severity of intraoperative trauma, development of bronchopulmonary complications, lack of compression, and healing of cavities.Results. No significant difference in the degree of intraoperative injury was found when comparing three types of thoracoplasty (p > 0.05). Clinical and X-ray examinations of patients in 3 weeks after surgery revealed that in thoracoplasty with a polypropylene mesh, the frequency of bronchopulmonary complications and lack of compression was lower than in other types of thoracoplasty (p < 0.05). Using the patient's own tissues for extrapleural fixation, especially with large cavities, results in a significant decrease in the thoracoplasty efficacy.

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Comparison of Chest X-Ray Findings Between Primary and Secondary Multidrug Resistant Pulmonary Tuberculosis

BioScientia Medicina ◽

10.32539/bsm.v5i4.356 ◽

2021 ◽

Vol 5 (4) ◽

pp. 855-862

Author(s):

Ricky Septafianty ◽

Anita Widyoningroem ◽

M. Yamin S. S ◽

Rosy Setiawati ◽

Soedarsono

Keyword(s):

Pulmonary Tuberculosis ◽

Multidrug Resistant ◽

X Rays ◽

Pulmonary Tb ◽

X Ray ◽

Pleural Thickening ◽

Significant Difference ◽

Record Data ◽

Mdr Tb ◽

Chest X Ray

Introduction: Radiological imaging has a key role in multidrug-resistant (MDR) pulmonary tuberculosis (TB) screening and diagnosis. However, new cases of MDR pulmonary TB are often overlooked; therefore, its transmission might continue before its diagnosis. The most widely used and affordable radiological modality is a chest radiograph. This study aims to describe the characteristics of primary and secondary MDR pulmonary TB chest x-ray findings for differential diagnosis. Methods: This study was an analytic observational study with a retrospective design. Researchers evaluated medical record data of primary and secondary MDR pulmonary TB patients who underwent chest x-ray examinations. The patient's chest x-rays were then evaluated. Evaluated variables were lung, pleural, and mediastinal abnormalities and severity category. Results: The most common chest x-ray finding in primary MDR pulmonary TB was consolidation (96.2%), which was mostly unilateral (52.0%), accompanied by cavities (71.2%), most of which were multiple (83.8%) with a moderate category of severity. The most common chest x-ray finding in secondary MDR pulmonary TB was consolidation (100%), which was mostly bilateral (60.4%), accompanied by cavities (80.2%), most of which were multiple (90.1%) with severe category of severity. Pleural thickening (47.5%) was also found. Conclusion: There was a significant difference between primary and secondary MDR pulmonary TB in terms of mild severity category, and pleural thickening. Mild severity category is mostly found in primary MDR-TB and pleural thickening is mostly found in secondary TB.

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Multiple Pulmonary Calssified Nodules and Cavitation Filled With the Dense Body on Chest X-Ray: A Case Report

International Journal of Medical Toxicology and Forensic Medicine ◽

10.32598/ijmtfm.v9i4.25106 ◽

2019 ◽

Vol 9 (4) ◽

pp. 255-260

Author(s):

Khosrow Agin ◽

Marymo Sadat Moin Azad Tehrani ◽

Bita Dadpour ◽

Maryam Vahabzadeh ◽

Babak Mostafazadeh

Keyword(s):

Differential Diagnosis ◽

Case Report ◽

Clinical Study ◽

Pulmonary Tuberculosis ◽

Clinical Assessment ◽

Dense Body ◽

Pulmonary Diseases ◽

X Ray ◽

Chest X Ray ◽

Initial Imaging

Imaging in the clinical study has a significant value in medical diagnosing and the following diseases. The characteristic signs of imaging are a clue in approach to differential diagnosis. A conventional chest x-ray radiography is a more prevalent image used in the initial clinical assessment of pulmonary diseases. Iran is one of the 9 countries with a 95% burden of tuberculosis in the Eastern Mediterranian region. Chest radiography is the initial imaging for the evaluation of pulmonary tuberculosis.

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Determination some Immunological Aspects in Pulmonary Tuberculosis Patients in Qadisiyah Province

International Journal of Pharmaceutical Quality Assurance ◽

10.25258/ijpqa.v9i2.13632 ◽

2018 ◽

Vol 9 (2) ◽

Author(s):

Syoof Khowman Alramahy ◽

Akram Hadi Hamza

Keyword(s):

Mycobacterium Tuberculosis ◽

Pulmonary Tuberculosis ◽

Statistical Difference ◽

Positive Culture ◽

Control Group ◽

Tuberculosis Patients ◽

Significant Difference ◽

Lowenstein Jensen ◽

The Mean ◽

Igg Level

This study was carried out to study of some immunological aspects among the pulmonary Tuberculosis patients infected with causative agent, Mycobacterium tuberculosis. A Total of 200 sputum samples were collected from patients attending the consultant Clinic for Chest and Respiratory disease center, Diwaniya. Control group (No=15) also included. According to acid fast stain of sputum, the patients were classified as positive (No=91,45.5%) and negative (No=109,54.5, Lowenstein Jensen medium used for the cultivation of samples, on which 70% of sputum samples where positive culture for this microorganism. The grown microorganism were identified as M. tuberculosis, based on positive A.F.B, Niacin producers ,negative for catlase at 68c. The mean IgG level was l184.053±76.684 mg/100 ml in tuberculosis group compared with 1016.533 ± 44.882 mg/100ml in control group, rendering the statistical difference significant. For IgA and IgM levels, they were at mean of 315.880±38.552 mg/100 ml and 119.527±8.464 mg/100 ml in control group compared with 396.358±38.776 mg/100 ml and 134.207±11.696 mg/100 ml in patients group respectively with significant difference

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Crystal structure of pimecrolimus Form B, C43H68ClNO11

Powder Diffraction ◽

10.1017/s088571562100004x ◽

2021 ◽

Vol 36 (1) ◽

pp. 35-42

Author(s):

Shivang Bhaskar ◽

Joseph T. Golab ◽

James A. Kaduk ◽

Amy M. Gindhart ◽

Thomas N. Blanton

Keyword(s):

Crystal Structure ◽

Powder Diffraction ◽

Density Functional ◽

Van Der Waals Interactions ◽

Powder Diffraction File ◽

X Ray ◽

Weak Intermolecular Interactions ◽

Significant Difference ◽

Atomic Coordinates ◽

Solid State Conformation

The crystal structure of pimecrolimus Form B has been solved and refined using synchrotron X-ray powder diffraction data and optimized using density functional techniques. Pimecrolimus crystallizes in the space group P21 (#4) with a = 15.28864(7), b = 13.31111(4), c = 10.95529(5) Å, β = 96.1542(3)°, V = 2216.649(9) Å3, and Z = 2. Although there are an intramolecular six-ring hydrogen bond and some larger chain and ring patterns, the crystal structure is dominated by van der Waals interactions. There is a significant difference between the conformation of the Rietveld-refined and the DFT-optimized structures in one portion of the macrocyclic ring. Although weak, intermolecular interactions are apparently important in determining the solid-state conformation. The powder pattern is included in the Powder Diffraction File™ (PDF®) as entry 00-066-1619. This study provides the atomic coordinates to be added to the PDF entry.

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AB0009 ASSOCIATION OF STAT4 RS7574865, RUNX1 RS9979383, IL6 RS1800795, IL6R RS2228145, IL6R RS4845618 WITH SYSTEMIC LUPUS ERYTHEMATOSUS SUSCEPTIBILITY AND SOME LUPUS MANIFESTATIONS IN BELARUSIAN POPULATION

Annals of the Rheumatic Diseases ◽

10.1136/annrheumdis-2021-eular.2523 ◽

2021 ◽

Vol 80 (Suppl 1) ◽

pp. 1039.2-1040

Author(s):

N. Dostanko ◽

V. Yagur ◽

R. Goncharova ◽

E. Siniauskaya ◽

T. Zybalova

Keyword(s):

Systemic Lupus Erythematosus ◽

Lupus Erythematosus ◽

Diagnostic Odds Ratio ◽

Protective Role ◽

Fisher Exact Test ◽

Systemic Lupus ◽

Exact Test ◽

American College Of Rheumatology ◽

Healthy Donors ◽

Significant Difference

Background:Systemic lupus erythematosus (SLE) has a significant genetic predisposition. Many genetic variants of susceptibility to SLE have been published and analyzed, but the clinical and functional significance of the various genotypes has not yet been clearly defined [1].Objectives:To estimate the association between some of non-HLA gene polymorphisms such as STAT4 rs7574865, RUNX1 rs9979383, IL6 rs1800795, IL6R rs2228145, IL6R rs4845618 and susceptibility to SLE in Belarusian population as well as some disease manifestations.Methods:We examined 383 healthy blood donors and 54 SLE patients (18-72 years old, median age 35) classified according to the 1997 American College of Rheumatology (ACR) revised classification criteria [2]. Deoxyribonucleic acid was extracted from peripheral blood samples by phenol-chloroform method. Genotyping was performed by real-time PCR with fluorescent probes. Differences of distribution of all the single nucleotide polymorphism (SNP) genotypes and their associations with secondary antiphospholipid syndrom (APS) and lupus arthritis were analyzed using Pearson χ2 (χ2) and two-way Fisher exact test (F, p2-t). Diagnostic odds ratio (dOR), likelihood ratio of positive (LR +) and negative (LR –) tests and corresponding 95% confidence intervals (CI) were also calculated.Results:We revealed significant difference in STAT4 rs7574865 genotypes in SLE patients and healthy donors (χ2=8,27, р=0,016) with significant increase of ТТ genotype frequency in SLE patients vs healthy donors (χ2=6.83 p=0.009; p2-t =0.020; dOR=3.78 (CI95% 1.36-10.55); LR+ =3.44 (CI95% 1.35-8.71); LR– =0.91 (CI95% 0.83-0.98)). Lupus arthritis was more common in risk TT-genotype SLE carriers than in other SLE patients (χ2=5.902 p=0.015; p2-t =0.027).We revealed significant increase of СТ genotype (RUNX1 rs9979383) in healthy donors vs SLE patients (χ2=4.14; p=0.042; dOR=0.53 (CI95% 0.29-0.98); LR+ =0.69 (CI95% 0.45-0.99); LR– =1.3 (CI95% 1.01-1,56)). Lupus arthritis was more common in SLE СТ-genotype carriers than in other SLE patients (χ2=4.66 p=0.031; p2-t =0.058).Significant differences in IL6 rs1800795, IL6R rs2228145 and IL6R rs4845618 genotypes distribution between studied groups were not found (χ2, p=0.427, p=0.559 and p=0.407, correspondingly) but GG-genotype (IL6 rs1800795) carriership in SLE patients was associated with increased APS frequency (χ2=4.45, p=0.035; dOR=0.19 (CI95% 0.04-0.9); LR+ =0.28 (CI95% 0.07-0.93); LR– =1.41 (CI95% 1.03-1.64).Conclusion:Our data suggest the susceptibility to SLE in ТТ genotype of STAT4 rs7574865 polymorphism, protective role of СТ genotype of RUNX1 rs9979383 for SLE and association between GG-genotype of IL6 rs1800795 and APS in SLE patients in Belarusian population. Lupus arthritis was associated with ТТ genotype of STAT4 rs7574865 and СТ genotype of RUNX1 rs9979383.References:[1]Chen L, Morris DL, Vyse TJ. Genetic advances in systemic lupus erythematosus: an update. Curr Opin Rheumatol 2017;29:423–33.[2]Hochberg MC. Updating the American College of Rheumatology Revised Criteria for the classification of Systemic Lupus Erythematosus. Arthritis Rheum 1997;40:1725.Disclosure of Interests:None declared

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Integration of Dental Implants in Conjunction with EDTA-Conditioned Dentin Grafts: An Experimental Study

Dentistry Journal ◽

10.3390/dj9060063 ◽

2021 ◽

Vol 9 (6) ◽

pp. 63

Author(s):

Payam Farzad ◽

Ted Lundgren ◽

Adel Al-Asfour ◽

Lars Andersson ◽

Christer Dahlin

Keyword(s):

Direct Contact ◽

Mineral Content ◽

X Rays ◽

X Ray ◽

Dentin Surface ◽

Significant Difference ◽

C And N ◽

Micro Implants ◽

Demineralized Dentin

This study was undertaken to investigate the integration of titanium micro-implants installed in conjunction with previously dentin-grafted areas and to study the morphological appearance, mineral content, and healing pattern of xenogenic EDTA-conditioned dentin blocks and granules grafted to cavities in the tibial bone of rabbits. Demineralized and non-demineralized dentin blocks and granules from human premolars were implanted into cavities prepared on the lateral aspects of the tibias of rabbits. After a healing period of six months, micro-implants were installed at each surgical site. Histological examinations were carried out after 24 weeks. Characterization of the EDTA-conditioned dentin blocks was performed by means of light microscopy, dental X-rays, scanning electron microscopy, and energy dispersive X-ray analysis (EDX). No implants were found to be integrated in direct contact with the dentin particles or blocks. On the EDTA-conditioned dentin surface, the organic marker elements C and N dominated, as revealed by EDX. The hydroxyapatite constituents Ca and P were almost absent on the dentin surface. No statistically significant difference was observed between the EDTA-conditioned and non-demineralized dentin, as revealed by BIC and BA. The bone-inductive capacity of the dentin material seemed limited, although demineralization by means of EDTA indicated higher BIC and BA values in conjunction with the installed implants in the area. A 12 h EDTA treatment did not fully decalcify the grafts, as revealed by X-ray analysis.

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