scholarly journals New Laboratory Criterion for the Differential Diagnosis of Sarcoidosis and Pulmonary Tuberculosis

2020 ◽  
Author(s):  
Anna Starshinova ◽  
Anna Malkova ◽  
Yulia Zinchenko ◽  
Natalia Basantsova ◽  
Igor Kudryavtsev ◽  
...  

Abstract Take-Home Message.This study provides a new diagnostic index based on the antibodies to mutated citrullinated vimentin concentration and the level of different B subsets. Determination of index over 5 Units proved to be characteristic of sarcoidosis, what might help to differentiate SD from TB.Abstract. In some cases there is a problem of differential diagnosis of sarcoidosis(SD) and tuberculosis(TB)because of the similarities in clinical, X-ray and laboratory features. The aim of this study was to search for new differential diagnostic criteria for sarcoidosis and tuberculosis by calculating the index, based on the level of autoantibodies to modified citrullinated vimentin (anti-MCV) and the level of B-cell subpopulations. These parameters were measured in patients with sarcoidosis (n = 93), tuberculosis (n = 40) and healthy donors (n = 40) using the ELISA and cytometry. The absence of a statistically significant difference when comparing the level of anti-MCV, the number of B-cells in SD and TB suggests that these changes may be characteristic of granulomatous diseases. The use of the formula Ds=([B-naïve%]\[B-memory%])*([B-CD38%]+[B-CD5%])/[anti-MCV] might allow to differentiate SD with an increase in the calculated index of more than 5 units with a sensitivity of 80.00% and specificity of 93.10% (AUC = 0.926).

2020 ◽  
Author(s):  
Anna Starshinova ◽  
Anna Malkova ◽  
Yulia Zinchenko ◽  
Natalia Basantsova ◽  
Igor Kudryavtsev ◽  
...  

Abstract In some cases there is a problem of differential diagnosis of sarcoidosis (SD) and tuberculosis (TB) because of the similarities in clinical, X-ray and laboratory features. The aim of this study was to search for new differential diagnostic criteria for sarcoidosis and tuberculosis by calculating the index, based on the level of autoantibodies to modified citrullinated vimentin (anti-MCV) and the level of B-cell subpopulations. These parameters were measured in patients with sarcoidosis (n = 93), tuberculosis (n = 28) and healthy donors (n = 40) using the ELISA and cytometry. The absence of a statistically significant difference when comparing the level of anti-MCV, the number of B-cells in SD and TB suggests that these changes may be characteristic of granulomatous diseases. The use of the formula Ds=([B-naïve%]\[B-memory%])*([B-CD38%]+[B-CD5%])/[anti-MCV] might allow to differentiate SD with an increase in the calculated index of more than 5 units with a sensitivity of 80.00% and specificity of 93.10% (AUC = 0.926).


1961 ◽  
Vol 7 (2) ◽  
pp. 115-129 ◽  
Author(s):  
Samuel Natelson ◽  
Bertrum Sheid

Abstract X-ray spectroscopy was applied to the determination of the total phosphorus content of serum and the iron content of whole blood (hemoglobin). Samples were placed on confined spots on paper, dried, then passed through the X-ray field. Concentration was then determined from the peaks as drawn on a recorder. A device is described that permits automatic assay of successive samples dried on paper without delays between readings. Samples are placed in the field and rapidly withdrawn at 30-sec. intervals, the response of the recorder serving as a measure of concentration. Results for total phosphorus in serum and total iron in whole blood were compared with those obtained by a wet ashing procedure. No significant difference with respect to both mean values and precision was observed except that total iron by the wet ashing procedure was more precise. However, the X-ray technic was adequate for clinical purposes. Hemoglobin levels calculated from whole-blood iron values demonstrated that bilirubin, red cell debris, leukocytes, and lipids interfered in the colorimetric but not in the X-ray procedure. A hemoglobinometer based on the X-ray spectroscopic technic may, therefore, yield results of greater clinical significance.


2018 ◽  
Vol 2018 ◽  
pp. 1-11
Author(s):  
L. M. Romero-Guerrero ◽  
R. Moreno-Tovar ◽  
A. Arenas-Flores ◽  
Y. Marmolejo Santillán ◽  
F. Pérez-Moreno

In the present work, the chemical, mineralogical, refractory, and microstructural characterizations of kaolinites from the Huayacocotla-Alumbres region, which is between Veracruz and Hidalgo border, by X-ray diffraction (XRD), polarization optical microscopy (POM), scanning electron microscopy (SEM), refractoriness proof (pyrometric cone equivalent), and thermogravimetric analysis (TGA) were carried out. The analysis by POM showed that the kaolinization degree in this region is variable due to the presence of primary minerals, such as plagioclase, feldspar, and quartz. Additionally, hydrothermal alteration of the epithermal type was determined by oxidation of sulfides (pyrite and galena) and chlorite association. With the X-ray diffraction technique, andalusite and kaolinite were identified as the majority phases in Huayacocotla and quartz was identified as the majority phase in Alumbres. The minority phases, such as dickite, kaolinite, and cristobalite, were observed in both zones. The SEM technique was useful in the determination of the morphology of kaolinite and impurities of Na, Mg, K, and Fe of the complex clay illite-andalusite-dickite group. Thermogravimetric analysis was useful to discover the decomposition temperature and reveal the significant difference between 400 and 800°C, which showcases the greatest mass loss due to dehydration and carbonates decomposition. The mullite phase was detected at approximately 1000°C in the kaolin samples. The refractoriness tests were important to determine the stability temperature of kaolin, which is between 1300 and 1600°C. This stability temperature makes it feasible to use the kaolin as a refractory material for both low and high temperatures. The variables that affect the kaolin stability temperature were determined by principal components with the XLSTAT free program.


1989 ◽  
Vol 03 (01) ◽  
pp. 93-99 ◽  
Author(s):  
C. MARTIN ◽  
D. BOURGAULT ◽  
C. MICHEL ◽  
M. HERVIEU ◽  
B. RAVEAU

Two new thallium cuprates isostructural with the “1212” superconductors were synthesized. These materials do not exhibit any superconductivity in spite of the presence of Tl(III), contrary to the oxides such as Tl 2 Ba 2 CaCu 2 O 8 or Tl 2 Ba 2 Ca 2 Cu 3 O 10. Their X-ray powder diffraction study confirms their close relationships with the superconductor TlBa 2 CaCu 2 O 7, i.e., their structure is built up from distorted double rock salt-type layers [(TIO) (BaO)] intergrown with oxygen deficient double perovskite layers [ BaY ( CuO 2.5)2] or [ BaNd ( CuO 2.5)2]. Although the determination of the positions of the oxygen atoms cannot be considered as accurate, one significant difference with respect to TlBa 2 CaCu 2 O 7 must be pointed out: the oxygen atoms forming the basal planes of the CuO 5 pyramids are brought closer together along c, leading to an almost cubic environment for Y and Nd, whereas the CaO 8 prisms are elongated along c in the thallium superconductors.


1964 ◽  
Vol BME-11 (3) ◽  
pp. 67-72 ◽  
Author(s):  
H. C. Becker ◽  
W. J. Nettleton ◽  
P. H. Meyers ◽  
J. W. Sweeney ◽  
C. M. Nice

1963 ◽  
Vol 7 ◽  
pp. 584-589
Author(s):  
M. C. Farquhar ◽  
M. M. English

AbstractA method is presented for the quantitative determination of thorium in monazite by fluorescent X-ray speetrometry. Strontium is used as an internal standard, and sample preparation consists of mechanically blending the finelyground (325-mesh) sample with reagent grade strontium carbonate. The prepared sample blend is packed into a sample cup and irradiated at 50 kV and 27 mA. A lithium fluoride analyzing crystal is used, and the intensity of the Th Lα1 and Sr Kα peaks are scaled. Intensities are corrected fov background. Calibration is achieved with a single sample of monazite of known thorium content. National Bureau of Standards monazite sample number 2601 (9.65% ThO2) was used in this work. The standard monazite is blended with strontium carbonate, irradiated, and scaled under the same conditions as the sample. The thorium content of the sample is then calculated by the equation: %ThO2 = (R sample) (%ThO2 standard)/R standard, where R = Thcounts/sec/Srcounts/sec. Replicate results agreed within 0.2% ThO2 on six samples of monazite which contained from 4.4% to 6.8% ThO2. Independent results by a second laboratory show no significant difference in precision.The method was used to determine the thorium content gf several rare-earth materials which contained approximately 0.5% to 1.0% TriOs. The results agree closely with values obtained by gamma-ray spectrometry.


2020 ◽  
Vol 644 ◽  
pp. A78
Author(s):  
L. Lovisari ◽  
S. Ettori ◽  
M. Sereno ◽  
G. Schellenberger ◽  
W. R. Forman ◽  
...  

Context. Total mass is arguably the most fundamental property for cosmological studies with galaxy clusters. The individual cluster masses can be obtained with different methods, each with its own biases and limitations. Systematic differences in mass measurements can strongly impact the determination of the hydrostatic bias and of the mass-observable relations, key requirements of many cluster abundance studies. Aims. We investigate the present differences in the mass estimates obtained through independent X-ray, weak-lensing, and dynamical studies using a large subsample of the Planck-ESZ clusters. We also discuss the implications for mass bias analyses. Methods. After assessing the systematic differences in the X-ray-derived masses reported by distinct groups, we examine the mass estimates obtained with independent methods and quantify the differences as the mean ratio 1-b = MHE/MWL, dyn, where HE refers to hydrostatic masses obtained from X-ray observations, WL refers to the results of weak-lensing measurements, and dyn refers to the mass estimates either from velocity dispersion or from the caustic technique. So defined, the 1-b parameter includes all possible astrophysical, observational, and methodological biases in one single value. Results. Recent X-ray masses reported by independent groups show average differences smaller than ∼10%, posing a strong limit on the systematics that can be ascribed to the differences in the X-ray analysis when studying the hydrostatic bias. The mean ratio between our X-ray masses and the weak-lensing masses in the LC2-single catalog is 1-b = 0.74 ± 0.06, which corresponds to a mass bias of 26 ± 6%, a value insufficient to reconcile the Planck cluster abundance and cosmic microwave background results. However, the mean mass ratios inferred from the WL masses of different projects vary by a large amount, with APEX-SZ showing a bias consistent with zero (1-b = 1.02 ± 0.12), LoCuSS and CCCP/MENeaCS showing a significant difference (1-b = 0.76 ± 0.09 and 1-b = 0.77 ± 0.10, respectively), and WtG pointing to the largest deviation (1-b = 0.61 ± 0.12), which would substantially reduce the tension between the Planck results. Because of small differences between our M − YX relation and the one used by the Planck collaboration, our X-ray masses are on average 7% lower (4% at the same physical radius) than the Planck masses and can further reduce the required bias. At odds with the WL results, the dynamical mass measurements show better agreement with the X-ray hydrostatic masses, although there are significant differences when relaxed or disturbed clusters are used. However, the comparison is currently limited by the small sample sizes. Conclisions. The systematic differences between total masses obtained with recent independent X-ray analyses are smaller than those found in previous studies. This shifts the focus to WL and dynamical studies for a better convergence of the level of mass bias. However, the ratios obtained using different mass estimators suggest that there are still systematics that are not accounted for in all the techniques used to recover cluster masses. This prevents the determination of firm constraints on the level of hydrostatic mass bias in galaxy clusters.


Author(s):  
Reham A. Mostafa ◽  
Doaa R. Negm ◽  
Naser A. ElSawy

Background: Dual energy X-ray absorptiometry (DEXA) is the most accurate technique in determining fat percentage but it is unpotable, expensive, unavailable for general applicability. Objective: This study aimed to find the most accurate and easiest technique as alternative to DEXA for quick determination of body fat%. This study examined the accuracy of three models of Bioelectrical impedance analysis (BIA) technique (Fat Loss Monitor- Body Composition -Body Fat Analyzer) in determining the body fat percentage with using DEXA as a reference standard. Subject and Methods: A cross sectional study was carried out during the period from (28-9-2017) to (5-4-2018) among a random sample of (53) volunteers female student in Umm Al Qura University from Faculty of Applied Medical Science aged between (20-39 years) from different level of education. All subjects were generally healthy, data was collected through a structured questionnaire compose of three section. Demographic data was collected and anthropometric measurements were evaluated as well as body composition (Only body fat%) using DEXA, body composition monitor, fat loss monitor and body fat analyzer. Results: BMI classification of the total 53 subjects was including 8 healthy females, 17 overfat, 25 obese and 3 underweight. The fat% result from DEXA and body fat analyzer was significant difference at (P < 0.05). Fat percent result from DEXA and Body Composition device was non-significant difference at (P>0.05). The fat% result from DEXA and Fat Loss Monitor device was significant at difference (P<0.05). Conclusion: The most accurate of BIA technique was the Body Composition device compering to DEXA.


Author(s):  
H.J. Dudek

The chemical inhomogenities in modern materials such as fibers, phases and inclusions, often have diameters in the region of one micrometer. Using electron microbeam analysis for the determination of the element concentrations one has to know the smallest possible diameter of such regions for a given accuracy of the quantitative analysis.In th is paper the correction procedure for the quantitative electron microbeam analysis is extended to a spacial problem to determine the smallest possible measurements of a cylindrical particle P of high D (depth resolution) and diameter L (lateral resolution) embeded in a matrix M and which has to be analysed quantitative with the accuracy q. The mathematical accounts lead to the following form of the characteristic x-ray intens ity of the element i of a particle P embeded in the matrix M in relation to the intensity of a standard S


Author(s):  
R. J. Narconis ◽  
G. L. Johnson

Analysis of the constituents of renal and biliary calculi may be of help in the management of patients with calculous disease. Several methods of analysis are available for identifying these constituents. Most common are chemical methods, optical crystallography, x-ray diffraction, and infrared spectroscopy. The application of a SEM with x-ray analysis capabilities should be considered as an additional alternative.A scanning electron microscope equipped with an x-ray “mapping” attachment offers an additional dimension in its ability to locate elemental constituents geographically, and thus, provide a clue in determination of possible metabolic etiology in calculus formation. The ability of this method to give an undisturbed view of adjacent layers of elements in their natural state is of advantage in determining the sequence of formation of subsequent layers of chemical constituents.


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