scholarly journals Metabolomics Study of Dried Ginger Extract on Serum and Urine in Blood Stasis Rats Based on UPLC-Q-TOF/MS

2020 ◽  
Author(s):  
Min Su ◽  
Gang Cao ◽  
Xiaoli Wang ◽  
Raftery Daniel ◽  
Yan Hong ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Metabolic Pathways ◽  
Blood Stasis ◽  
Blood Stasis Syndrome ◽  
High Dose ◽  
Therapeutic Effects ◽  
Ginger Extract ◽  
Multivariate Statistical ◽  
Metabolomics Study ◽  
Serum And Urine

Abstract Backgroud: According to the theory of traditional Chinese medicine (TCM), blood stasis syndrome (BSS) is the basis for many cardiovascular diseases. Ginger is often used as Chinese medicine (herb medicine) in China and Southeast Asia for the treatment of cardiovascular, respiratory, and digestive diseases. but the specific mechanism is unclear.Methods: After establishing an acute rat model of blood stasis syndrome, blood and urine of 5 groups of rats were collected for analysis of efficacy indicators. Aortic vessels are used for pathological testing. Serum and urine were used for metabolomics, multivariate statistical analysis was used to explore metabolites and metabolic pathways, and the correlation between metabolites and pharmacodynamic indicators was further explored.Results: In this experiment, the model of blood stasis syndrome was successfully established based on changes in vascular disease and the efficacy index. The experimental results show that the efficacy indicators of dried ginger (DG) extracts of different doses have different degrees of changes than model group (MG), and the high dose of dried ginger group (GJH) changes are the most significant (p < 0.05 or p < 0.01). And 22 metabolites (10 in serum and 12 in urine) were identified and contributed to the blood stasis progress. These metabolites mainly involve seven metabolism pathways in different impact-value. Dried ginger has therapeutic effects on BSS rats by regulating multiple metabolic pathways.Conclusion: This study provides an effective method for understanding the metabolic mechanism of dried ginger extracts on BSS.

scholarly journals Therapeutic Effect and Mechanism Study of Rhodiola wallichiana var. cholaensis Injection to Acute Blood Stasis Using Metabolomics Based on UPLC-Q/TOF-MS

2019 ◽  
Vol 2019 ◽  
pp. 1-20 ◽  
Author(s):  
Nan Ran ◽  
Zhiqiang Pang ◽  
Xuewa Guan ◽  
Guoqiang Wang ◽  
Jinping Liu ◽  
...  
Keyword(s):  
Rat Model ◽  
Metabolic Pathways ◽  
Acid Metabolism ◽  
Blood Stasis ◽  
Blood Stasis Syndrome ◽  
Inflammatory Factors ◽  
Control Group ◽  
High Dose ◽  
Alpha Linolenic Acid ◽  
Model Control

In traditional Chinese medicine theory, blood stasis syndrome (BSS), characterized by blood flow retardation and blood stagnation, is one of the main pathologic mechanisms and clinical syndromes of cardiovascular diseases (CVDs). Rhodiola wallichiana var. cholaensis injection (RWCI) is made from dry roots and stems of RWC via the processes of decoction, alcohol precipitation, filtration, and dilution. Studies indicated the extracts of RWC could alleviate CVDs; however, the mechanism had not been illustrated. In the present study, the acute blood stasis rat model was established to investigate the pathogenesis of BSS and the therapeutic mechanism of RWCI against BSS. Hemorheological parameters (whole blood viscosity and plasma viscosity) and inflammatory factors (TNF-α and IL-6) were used to evaluate the success of the BSS rat model and RWCI efficacy. 14 and 33 differential metabolites were identified from plasma and urine samples using the metabolomics approach based on ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. The results of multivariate analysis displayed that there were significant separations among model, control, and treatment groups, but the high-dose RWCI treatment group was closer to the control group. 9 perturbed metabolic pathways were related to BSS’s development and RWCI intervention. 5 metabolic pathways (arachidonic acid metabolism, linoleic acid metabolism, alpha-linolenic acid metabolism, retinol metabolism, and steroid hormone biosynthesis) showed apparent correlations. These differential metabolites and perturbed metabolic pathways might provide a novel view to understand the pathogenesis of BSS and the pharmacological mechanism of RWCI.

scholarly journals Metabolomics study of dried ginger extract on serum and urine in blood stasis rats based on UPLC‐Q‐TOF/MS

2020 ◽  
Vol 8 (12) ◽  
pp. 6401-6414
Author(s):  
Min Su ◽  
Gang Cao ◽  
Xiaoli Wang ◽  
Raftery Daniel ◽  
Yan Hong ◽  
...  
Keyword(s):  
Blood Stasis ◽  
Ginger Extract ◽  
Metabolomics Study ◽  
Tof Ms ◽  
Serum And Urine

scholarly journals A high-throughput metabolomics strategy for discovering the influence of differential metabolites and metabolic pathways of huaxian capsules on sepsis-associated Qi deficiency and blood stasis syndrome

RSC Advances ◽  
2019 ◽  
Vol 9 (53) ◽  
pp. 30868-30878
Author(s):  
Qun Liang ◽  
Han Liu ◽  
Xiu-li Li ◽  
Pei-yang Sun ◽  
Yang Yang ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
High Throughput ◽  
Metabolic Pathways ◽  
Blood Stasis ◽  
Therapeutic Targets ◽  
Blood Stasis Syndrome

High-throughput metabolomics can be used to investigate the therapeutic targets and metabolic mechanisms of traditional Chinese medicine formulae.

The therapeutic effect and mechanism of Qishen Yiqi dripping pills on cardiovascular and cerebrovascular diseases and diabetic complications

2021 ◽  
Vol 14 ◽  
Author(s):  
Chunlai Zhao ◽  
Wenjia Wang ◽  
Kaijing Yan ◽  
He Sun ◽  
Jihong Han ◽  
...  
Keyword(s):  
Coronary Heart Disease ◽  
Diabetic Nephropathy ◽  
Heart Disease ◽  
Ischemic Stroke ◽  
Diabetic Complications ◽  
Blood Stasis ◽  
Blood Stasis Syndrome ◽  
Syndrome Differentiation ◽  
Therapeutic Effects ◽  
The Common

: The alterations in vascular homeostasis is deeply involved in the development of numerous diseases, such as coronary heart disease, stroke, and diabetic complications. Changes in blood flow and endothelial permeability caused by vascular dysfunction are the common mechanisms for these three types of diseases. The disorders of glucose and lipid metabolism can result in changes of the energy production patterns in endothelium and surrounding cells which may consequently cause local energy metabolic disorders, oxidative stress and inflammatory responses. Traditional Chinese medicine (TCM) follows the principle of the “treatment by the syndrome differentiation”. TCM considers of that coronary heart disease, stroke and diabetes complications all as the type of “Qi deficiency and Blood stasis” syndrome, which mainly happens to the vascular system. Therefore, the common pathogenesis of these three types of diseases suggests the treatment strategy by TCM should be in a close manner and named as “treating different diseases by the same treatment”. Qishen Yiqi dripping pills is a modern Chinese herbal medicine which has been widely used for treatment of patients with coronary heart disease characterized as “Qi deficiency and blood stasis” in China. Recently, many clinical reports have demonstrated the potent therapeutic effects of Qishen Yiqi dripping pills on ischemic stroke and diabetic nephropathy. Based on these reports, we will summarize the clinical applications of Qishen Yiqi dripping pills on coronary heart disease, ischemic stroke and diabetic nephropathy, including the involved mechanisms with basic researches.

scholarly journals Therapeutic Effects of Traditional Chinese Medicine Formula Qianlie Tongli Decoction on Chronic Prostatitis/Chronic Pelvic Pain Syndrome Induced by Peptide T2 in Mice

2020 ◽  
Author(s):  
Xingxing Cui ◽  
Muhammad Naveed ◽  
Mirza Muhammad Faran Ashraf Baig ◽  
Wenlu Wang ◽  
Kashif Dar ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Pelvic Pain ◽  
Dose Group ◽  
Chronic Pelvic Pain Syndrome ◽  
High Dose Group ◽  
Pelvic Pain Syndrome ◽  
High Dose ◽  
Therapeutic Effects ◽  
Chinese Medicine Formula ◽  
Medium Dose

Objectives: This study was undertaken to reveal therapeutic effects and the preliminary mechanism of Chinese medicine formula Qianlie Tongli decoction (QTD) in chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS). Methods: A total of 50 male C57BL/6 mice were randomly divided into five groups. All groups except the control group were injected subcutaneously T2 peptide emulsion, which induced the CP/CPPS model. After the induction of CP/CPPS, the model group was given 0.9% NaCl by oral gavage while low-dose, medium-dose, and high-dose groups were treated with Chinese medicine formula. Micturition habits and pain behavior of mice were analyzed for each group. Hematoxylin and eosin staining were used to investigate prostate inflammation. The serum level of tumor necrosis factor-&alpha; (TNF-&alpha;) was measured by enzyme-linked immunosorbent assay (ELISA) kit. Key findings: Chinese medicine formula significantly reduced the number of urine spots and improved pain response frequency in the medium-dose and high-dose group. The high-dose group showed reduced considerably inflammatory lesion and inflammatory cell infiltration than the low-dose and medium-dose groups. Serum levels of TNF-&alpha; in the high-dose group were significantly reduced compared with the model group. Conclusions: The results demonstrated the therapeutic effects of Qianlie Tongli decoction in CP/CPPS mice by analyzing clinically relevant symptoms (urinary tract system, pelvic pain, and prostate inflammation), and preliminary explored the inflammatory-related treatment mechanisms by measuring TNF-&alpha;.

BUILDING AND EVALUATING AN ANIMAL MODEL FOR SYNDROME IN TRADITIONAL CHINESE MEDICINE IN THE CONTEXT OF UNSTABLE ANGINA (MYOCARDIAL ISCHEMIA) BY SUPERVISED DATA MINING APPROACHES

2009 ◽  
Vol 17 (04) ◽  
pp. 531-546 ◽  
Author(s):  
SHUZHEN GUO ◽  
JIANXIN CHEN ◽  
HUIHUI ZHAO ◽  
WEI WANG ◽  
JIANQIANG YI ◽  
...  
Keyword(s):  
Data Mining ◽  
Animal Model ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Myocardial Ischemia ◽  
Unstable Angina ◽  
Clinical Epidemiology ◽  
Blood Stasis ◽  
Blood Stasis Syndrome ◽  
Novel Strategy

Building an animal model for a disease is a better avenue to understand the inner mechanism of it. Traditional Chinese Medicine accumulated much practical experience and a large amount of literature to heal diseases during the past 3000 years. However, as there is no available animal model for TCM research because syndrome, the core of TCM theory, it is hard to be diagnosed from animals. In this paper, we present a novel strategy to build and evaluate an animal model for syndrome in TCM in the context of a disease. We first carried out a clinical epidemiology survey for a syndrome (Blood stasis syndrome, BSS) diagnosed by TCM experts in the context of a disease (Unstable angina, UA). Meanwhile, the blood samples of patients included in the survey were collected and measured as physical and chemical specifications by laboratory examinations. Alternatively, we used supervised data mining methods to build association between the specifications and the syndrome in the context of UA. The accuracy of classification was used to evaluate performance of the association built. Finally, we built an animal model for myocardial ischemia and validated the model by established diagnosis criterion of myocardial ischemia. Furthermore, the built association was used to evaluate whether an animal is with BSS. The results indicated that the strategy successfully evaluates and separates the animal model for syndrome in TCM from the counterpart for myocardial ischemia. The novel strategy presented in the paper provides a better insight to understand the nature of syndrome in TCM and pave a basis for personalized therapies of UA.

scholarly journals Holistic and dynamic metabolic alterations of traditional Chinese medicine syndrome in a toxic heat and blood stasis syndrome rat model

RSC Advances ◽  
2017 ◽  
Vol 7 (89) ◽  
pp. 56471-56483
Author(s):  
Jing-Jing Xu ◽  
Feng Xu ◽  
Shu-Jie Shen ◽  
Teng Li ◽  
Yi-Fan Zhang ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Rat Model ◽  
Blood Stasis ◽  
Blood Stasis Syndrome ◽  
Chinese Medicine Syndrome ◽  
Dynamic Changes ◽  
Metabolic Alterations ◽  
Traditional Chinese Medicine Syndrome ◽  
First Time

Dynamic changes of the metabolic network during the evolution of a syndrome based on the toxic heat and blood stasis syndrome (THBSS) rat model have been elucidated for the first time.

scholarly journals The Expression of CD14+CD16+Monocyte Subpopulation in Coronary Heart Disease Patients with Blood Stasis Syndrome

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Ye Huang ◽  
Jing-Shang Wang ◽  
Hui-jun Yin ◽  
Ke-ji Chen
Keyword(s):  
Coronary Heart Disease ◽  
Heart Disease ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Plaque Rupture ◽  
Blood Stasis ◽  
Blood Stasis Syndrome ◽  
Pathological State ◽  
Significant Difference ◽  
Gene Level

Blood stasis syndrome (BSS), a comprehensive pathological state, is one of the traditional Chinese medicine syndromes of coronary heart disease (CHD). In our previous study, we investigated that FcγRIIIA (also called CD14+CD16+monocyte subpopulation) is one of the differentially expressed genes related to CHD patients and its possible role in the atherosclerotic formation and plaque rupture. However, whether or not the deregulation of CD14+CD16+monocyte subpopulation expression is implicated in the pathogenesis of CHD patients with BSS has not yet been elucidated. In this study, we found that there was no significant difference between CHD patients with BSS and non-BSS in CD14+CD16+monocyte subpopulation at gene level. Moreover, the protein level of CD14+CD16+monocyte subpopulation in CHD patients with BSS was increased significantly when compared to the CHD patients with non-BSS. Additionally, the level of inflammatory cytokines downstream of CD14+CD16+monocyte subpopulation such as TNF-αand IL-1 in sera was much higher in CHD patients with BSS than that in CHD patients with non-BSS. Taken together, these results indicated that CD14+CD16+monocyte subpopulation was implicated in the pathogenesis of CHD patients with BSS, which may be one of the bases of the essence of BSS investigation.

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