scholarly journals Characterization of bacterial community of rumen from bovine during laminitis challenge by high-throughput sequencing

2020 ◽  
Author(s):  
Jian Guo ◽  
Haowen Sun ◽  
T. Maimai ◽  
Caijun Zhao ◽  
Yongguo Cao ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Bacterial Community ◽  
High Throughput Sequencing ◽  
Economic Loss ◽  
Ruminal Bacteria ◽  
Classic Problem ◽  
Bacterial Microbiota ◽  
Clostridium Papyrosolvens ◽  
Acid Metabolites

Abstract Background: Laminitis is a classic problem in the dairy industry, which can cause a great economic loss. However, the etiology and pathogenesis of laminitis have yet to be understood. In recent years, the microbiota has been the focus of much investigation in the search for various diseases. The present study aimed to explore the relationship between ruminal bacterial microbiota and laminitis. Results: The serum of healthy and laminitis bovines (n=8, respectively) collected from farms was used to detect concentrations of LPS, lactic acid, and histamine by the detection kits. This study used 16S rRNA sequencing to identify the differences in the bacterial community. The results showed that there was a significant increase in LPS and lactic acid in the laminitis group. Furthermore, microbial data analysis revealed that the laminitis group increased the abundance of bacteria with acid metabolites, such as Candidatus Saccharimonas, Saccharofermentans, Erysipelotrichaceae UCG-009, and Erysipelotrichaceae UCG-008, and [Clostridium] papyrosolvens and Ruminococcaceae bacterium AE2021.Conclusions: In summary, the changes in ruminal bacteria may potentially serve as the risk of laminitis.

scholarly journals Characterization of the Bacterial Community of Rumen in Dairy Cows with Laminitis

Genes ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1996
Author(s):  
Jian Guo ◽  
Ruiying Mu ◽  
Shuang Li ◽  
Naisheng Zhang ◽  
Yunhe Fu ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Rumen Fluid ◽  
Economic Loss ◽  
Control Group ◽  
16S Rdna Sequence ◽  
16S Rdna Sequence Analysis ◽  
The World ◽  
Clostridium Papyrosolvens ◽  
Ruminal Microbiota

Laminitis is the inflammation of the lamella, and it has caused great economic loss to the dairy industry and attracted wide attention around the world. In recent years, microbiota are considered to play a significant role in various diseases processes. Therefore, our study aimed to explore the characteristics of ruminal microbiota in laminitis cows. The serum of bovines with or without laminitis was collected to detect concentrations of lipopolysaccharide (LPS), lactic acid, and histamine, and ruminal fluid was collected for 16S rDNA sequence analysis. The results showed a significant increase in LPS and lactic acid levels in the laminitis group compared to the control group cows. In addition, a higher abundance of Candidatus Saccharimonas, Saccharofermentans, Erysipelotrichaceae UCG-009 genus, Acetobacter pasteurianus, Clostridium papyrosolvens, Ruminococcaceae bacterium AE2021, Porphyromonas crevioricanis, Pseudomonas boreopolis, Pseudomonas psychrotolerans, Rothia nasimurium, and Rothia pickettii was detected in the rumen fluid of laminitis bovines. In conclusion, this article confirms that there are differences in rumen microbiota between healthy and laminitis bovines. The elevated abundance of bacteria that enrich acid-enhancing metabolites, as well as increase the concentration of lactic acid and LPS, could be harmful factors to bovines and increase the risk of laminitis.

scholarly journals Characterization of bacterial community in the rumen of bovine with laminitis using high-throughput sequencing

2020 ◽  
Author(s):  
Jian Guo ◽  
Bo Liu ◽  
Tuniyazi. Maimai ◽  
Caijun Zhao ◽  
Yongguo Cao ◽  
...  
Keyword(s):  
Lactic Acid ◽  
High Throughput Sequencing ◽  
Rumen Fluid ◽  
Economic Loss ◽  
Control Group ◽  
16S Rrna Sequence ◽  
Pathological Mechanism ◽  
16S Rrna Sequence Analysis ◽  
Clostridium Papyrosolvens ◽  
Ruminal Microbiota

Abstract Background: Laminitis- an inflammation of lamella, could cause great economic loss to dairy industry, which has attracted wide attention around the world. Although previous researches explored risk factors of laminitis, the exact pathological mechanism of laminitis is still remaining unclear. In recent years, microbiota is considered as one of the vital parts that played significant role in various diseases processes. However, current studies are far from sufficient. Aim of this study is to explore the characteristics of ruminal microbiota in laminitis cows.Results: The serum of bovines with or without laminitis from farms was collected to detect concentrations of LPS, lactic acid, and histamine, and the ruminal fluid was collected for 16S rRNA sequence analysis. The results showed that there was a significant increase in LPS and lactic acid levels in laminitis group comparing to control group cows. In addition, the higher abundance of bacteria that -riches acid-enhancing metabolites, namely, Candidatus Saccharimonas, Saccharofermentans, Erysipelotrichaceae UCG-009, Erysipelotrichaceae UCG-008, Clostridium papyrosolvens and Ruminococcaceae bacterium AE2021 were detected in the rumen fluid from laminitis bovines.Conclusions: This article confirmed that difference of rumen microbiota were occurred in rumen between health and laminitis bovines. The elevated abundance of bacteria that riches acid- enhancing metabolites, as well as increased the concentration of lactic acid and LPS could be harmful factors to bovines and increase risks of laminitis.

scholarly journals Characterization of Bacterial Community of Rumen in Dairy Cows With Laminitis

2021 ◽  
Author(s):  
Jian Gao ◽  
Bo Liu ◽  
Shuang Li ◽  
Ruiying Mu ◽  
Naisheng Zhang ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Rumen Fluid ◽  
Economic Loss ◽  
Control Group ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
16S Rrna Sequence Analysis ◽  
Clostridium Papyrosolvens ◽  
Ruminal Microbiota

Abstract Background: Laminitis- an inflammation of lamella, could cause great economic loss to dairy industry, which has attracted wide attention around the world. In recent years, microbiota is considered as one of the vital parts that played significant role in various diseases processes. However, current studies are far from sufficient. Aim of this study is to explore the characteristics of ruminal microbiota in laminitis cows. Methods: The serum of bovines with or without laminitis was collected to detect concentrations of lipopolysaccharide (LPS), lactic acid, and histamine, and the ruminal fluid was collected for 16S rRNA sequence analysis. Results: The results showed that a significant increase in LPS and lactic acid levels in laminitis group comparing to control group cows. In addition, the higher abundance of bacteria that -riches acid-enhancing metabolites, namely, Candidatus Saccharimonas, Saccharofermentans, Erysipelotrichaceae UCG-009, Erysipelotrichaceae UCG-008, Clostridium papyrosolvens and Ruminococcaceae bacterium AE2021 were detected in the rumen fluid from laminitis bovines. Conclusions: This article confirmed that difference of rumen microbiota were occurred in rumen between health and laminitis bovines. The elevated abundance of bacteria that riches acid- enhancing metabolites, as well as increased the concentration of lactic acid and LPS could be harmful factors to bovines and increase risks of laminitis.

scholarly journals Enzyme additives influence bacterial communities of Medicago sativa silage as determined by Illumina sequencing

2020 ◽  
Author(s):  
Zongfu Hu ◽  
Deying Ma ◽  
huaxin Niu ◽  
Jie Chang ◽  
Jianhua Yu ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Bacterial Community ◽  
High Throughput ◽  
Bacterial Communities ◽  
Crude Protein ◽  
High Throughput Sequencing ◽  
Ammonium N ◽  
Enzyme Combination ◽  
Fermentation Quality ◽  
Dominant Bacteria

Abstract This study aimed to evaluate the effects of enzymes (cellulase combined with galactosidase),, and the combination of these enzymes with Lactobacillus plantarum (LP) on bacterial diversity using high-throughput sequencing. Alfalfa forages were treated without or with cellulase + ɑ-galactosidase (CEGA), cellulase + LP (CELP), ɑ-galactosidase + LP (GALP). After 56 days of ensiling, All the treated silages exhibited improved fermentation quality as reflecting by decreased pH, ammonium-N and increased lactic acid levels compared to the control silage. Enzymatic treatment improved nutrients value by increased the level of crude protein and decreased the neutral detergent fibre (NDF) level. Treatment of the silage significantly changed the bacterial community, as determined by the PCoA test. LAB dominated the bacterial community of the treated silage after ensiling. The dominant bacteria from Garciella, Enterococcus, Lactobacillus and Pediococcus in control silage changed to Lactobacillus and Pediococcus in CEGA silage, and Lactobacillus in CELP and GALP silages. Collectively, enzymes and enzyme in combination with inoculants both greatly increased the abundance of LAB, with Enterococcus, Lactobacillus and Pediococcus in enzymes only silge (CEGA) and Lactobacillus in enzyme combination with inoculants silage (CELP and GALP).

scholarly journals Characterization of the First Virulent Phage Infecting Oenococcus oeni, the Queen of the Cellars

2021 ◽  
Vol 11 ◽  
Author(s):  
Cécile Philippe ◽  
Amel Chaïb ◽  
Fety Jaomanjaka ◽  
Olivier Claisse ◽  
Patrick M. Lucas ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Lactic Acid ◽  
Bacterial Community ◽  
Malolactic Fermentation ◽  
Oenococcus Oeni ◽  
Common Species ◽  
Red Wines ◽  
Virulent Phage ◽  
Complete Genomic

There has been little exploration of how phages contribute to the diversity of the bacterial community associated with winemaking and may impact fermentations and product quality. Prophages of Oenococcus oeni, the most common species of lactic acid bacteria (LAB) associated with malolactic fermentation of wine, have been described, but no data is available regarding phages of O. oeni with true virulent lifestyles. The current study reports on the incidence and characterization of the first group of virulent oenophages named Vinitor, isolated from the enological environment. Vinitor phages are morphologically very similar to siphoviruses infecting other LAB. Although widespread during winemaking, they are more abundant in musts than temperate oenophages. We obtained the complete genomic sequences of phages Vinitor162 and Vinitor27, isolated from white and red wines, respectively. The assembled genomes shared 97.6% nucleotide identity and belong to the same species. Coupled with phylogenetic analysis, our study revealed that the genomes of Vinitor phages are architecturally mosaics and represent unique combinations of modules amongst LAB infecting-phages. Our data also provide some clues to possible evolutionary connections between Vinitor and (pro)phages associated to epiphytic and insect-related bacteria.

scholarly journals Effects of Lactic Acid Bacterial Inoculants on Fermentation Quality, Bacterial Community, and Mycotoxins of Alfalfa Silage under Vacuum or Nonvacuum Treatment

2021 ◽  
Vol 9 (12) ◽  
pp. 2614
Author(s):  
Xiaomiao Fan ◽  
Shanshan Zhao ◽  
Fengyuan Yang ◽  
Yuan Wang ◽  
Yanping Wang
Keyword(s):  
Lactic Acid ◽  
Microbial Community ◽  
Bacterial Community ◽  
High Throughput Sequencing ◽  
Ammonia Nitrogen ◽  
Positive Influence ◽  
Bacterial Inoculants ◽  
Fermentation Quality ◽  
Alfalfa Silage ◽  
Silage Quality

To investigate the effects of lactic acid bacterial (LAB) inoculants and vacuuming on the fermentation quality and bacterial community, alfalfas were ensiled with or without a commercial LAB YX or Lactobacillus plantarum strain ZZUA493 for 10, 30, 60, and 90 days while undergoing either vacuum (V) or nonvacuum (NV) treatment. At 90 days, analysis of the microbial community by high-throughput sequencing was performed, and contents of aflatoxin B1 and deoxynivalenol (DON) mycotoxins in alfalfa silage were determined. In all inoculated alfalfa silage, irrespective of V or NV treatment, lactic acid (LA) content increased, pH (p < 0.05), and ammonia nitrogen (p < 0.05) content decreased, and no butyric acid was detected. Lactobacillus or Pediococcus became the dominant genus, and the abundance of Garciella decreased in alfalfa silage with the addition of either inoculant. The LAB inoculants YX and ZZUA493 helped reduce the mycotoxin content in alfalfa silage. The abundance of Garciella in the control and DON content in all alfalfa silage groups were higher (p < 0.05) in NV than V. In summary, LAB inoculants and vacuuming had a positive influence on alfalfa silage quality, and LAB inoculants were effective in reducing mycotoxins in silage alfalfa.

scholarly journals Enzyme additives influence bacterial communities of Medicago sativa silage as determined by Illumina sequencing

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Zongfu Hu ◽  
Deying Ma ◽  
Huaxin Niu ◽  
Jie Chang ◽  
Jianhua Yu ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Bacterial Community ◽  
Bacterial Communities ◽  
Crude Protein ◽  
High Throughput Sequencing ◽  
Protein Levels ◽  
Nutrient Value ◽  
Ammonium N ◽  
Fermentation Quality ◽  
Dominant Bacteria

AbstractThe goal of the present study was to evaluate the effects of enzymes (cellulase combined with galactosidase) and their combination with Lactobacillus plantarum (LP) on bacterial diversity in alfalfa silages using high-throughput sequencing. Alfalfa forages were treated with or without cellulase + ɑ-galactosidase (CEGA), cellulase + LP (CELP), or ɑ-galactosidase + LP (GALP). After 56 days of ensiling, all treated silages exhibited improved fermentation quality, as reflected by decreased pH, ammonium-N and increased lactic acid levels compared to the control silage (P < 0.05). Enzymatic treatment improved nutrient value by increasing crude protein levels and decreasing neutral detergent fibre (NDF) levels (P < 0.05). Silage treatment significantly altered the bacterial community, as determined by PCoA (P < 0.05). Lactic acid bacteria (LAB) dominated the bacterial community of the treated silage after ensiling. The dominant bacteria changed from Garciella, Enterococcus, Lactobacillus and Pediococcus in the control silage to Lactobacillus and Pediococcus in the CEGA silage and Lactobacillus in the CELP and GALP silages. Collectively, these results suggest that treatment with both enzymes alone and in combination with inoculants greatly increased the abundance of LAB, with Enterococcus, Lactobacillus and Pediococcus observed in the silage treated with enzymes alone (CEGA) and Lactobacillus observed in the silage treated with a combination of enzymes and inoculants (CELP and GALP).

Characterization of bacterial diversity in drinking water by pyrosequencing

2013 ◽  
Vol 13 (2) ◽  
pp. 358-367 ◽  
Author(s):  
Yan Zhang ◽  
Qiang He
Keyword(s):  
Drinking Water ◽  
Bacterial Community ◽  
Bacterial Diversity ◽  
Clone Library ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Bacterial Community Composition ◽  
Environmental Distribution ◽  
Clone Library Analysis

Controlling microbial contamination of drinking water is critical to public health. However, understanding of the microbial ecology of drinking water remains incomplete. Representing the first application of high-throughput sequencing in drinking water microbiology, the objective of this study is to evaluate pyrosequencing as a high-throughput technique for the characterization of bacterial diversity in drinking water in comparison with conventional clone library analysis. Pyrosequencing and clone library analysis were performed in parallel to study the bacterial community composition in drinking water samples following the concentration of microbial biomass in drinking water with ultrafiltration. Validated by clone library analysis, pyrosequencing was confirmed as a highly efficient deep-sequencing technique to characterize the bacterial diversity in drinking water. Sequences of Alphaproteobacteria and Betaproteobacteria dominated the bacterial community in drinking water with Oxalobacteraceae and Methylobacteriaceae as the most abundant bacterial families, which is consistent with the prominent abundance of these populations frequently detected in various freshwater environments where source waters originate. Bacterial populations represented by the most abundant sequences in drinking water were closely related to cultures of metabolically versatile bacterial taxa widely distributed in the environment, suggesting a potential link between environmental distribution, metabolic characteristics, and abundance in drinking water.

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