Characterization of bacterial community in the rumen of bovine with laminitis using high-throughput sequencing

Abstract Background: Laminitis- an inflammation of lamella, could cause great economic loss to dairy industry, which has attracted wide attention around the world. Although previous researches explored risk factors of laminitis, the exact pathological mechanism of laminitis is still remaining unclear. In recent years, microbiota is considered as one of the vital parts that played significant role in various diseases processes. However, current studies are far from sufficient. Aim of this study is to explore the characteristics of ruminal microbiota in laminitis cows.Results: The serum of bovines with or without laminitis from farms was collected to detect concentrations of LPS, lactic acid, and histamine, and the ruminal fluid was collected for 16S rRNA sequence analysis. The results showed that there was a significant increase in LPS and lactic acid levels in laminitis group comparing to control group cows. In addition, the higher abundance of bacteria that -riches acid-enhancing metabolites, namely, Candidatus Saccharimonas, Saccharofermentans, Erysipelotrichaceae UCG-009, Erysipelotrichaceae UCG-008, Clostridium papyrosolvens and Ruminococcaceae bacterium AE2021 were detected in the rumen fluid from laminitis bovines.Conclusions: This article confirmed that difference of rumen microbiota were occurred in rumen between health and laminitis bovines. The elevated abundance of bacteria that riches acid- enhancing metabolites, as well as increased the concentration of lactic acid and LPS could be harmful factors to bovines and increase risks of laminitis.

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Characterization of Bacterial Community of Rumen in Dairy Cows With Laminitis

10.21203/rs.3.rs-942015/v1 ◽

2021 ◽

Author(s):

Jian Gao ◽

Bo Liu ◽

Shuang Li ◽

Ruiying Mu ◽

Naisheng Zhang ◽

...

Keyword(s):

Lactic Acid ◽

Rumen Fluid ◽

Economic Loss ◽

Control Group ◽

Rrna Sequence ◽

16S Rrna Sequence ◽

16S Rrna Sequence Analysis ◽

Clostridium Papyrosolvens ◽

Ruminal Microbiota

Abstract Background: Laminitis- an inflammation of lamella, could cause great economic loss to dairy industry, which has attracted wide attention around the world. In recent years, microbiota is considered as one of the vital parts that played significant role in various diseases processes. However, current studies are far from sufficient. Aim of this study is to explore the characteristics of ruminal microbiota in laminitis cows. Methods: The serum of bovines with or without laminitis was collected to detect concentrations of lipopolysaccharide (LPS), lactic acid, and histamine, and the ruminal fluid was collected for 16S rRNA sequence analysis. Results: The results showed that a significant increase in LPS and lactic acid levels in laminitis group comparing to control group cows. In addition, the higher abundance of bacteria that -riches acid-enhancing metabolites, namely, Candidatus Saccharimonas, Saccharofermentans, Erysipelotrichaceae UCG-009, Erysipelotrichaceae UCG-008, Clostridium papyrosolvens and Ruminococcaceae bacterium AE2021 were detected in the rumen fluid from laminitis bovines. Conclusions: This article confirmed that difference of rumen microbiota were occurred in rumen between health and laminitis bovines. The elevated abundance of bacteria that riches acid- enhancing metabolites, as well as increased the concentration of lactic acid and LPS could be harmful factors to bovines and increase risks of laminitis.

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Characterization of the Bacterial Community of Rumen in Dairy Cows with Laminitis

Genes ◽

10.3390/genes12121996 ◽

2021 ◽

Vol 12 (12) ◽

pp. 1996

Author(s):

Jian Guo ◽

Ruiying Mu ◽

Shuang Li ◽

Naisheng Zhang ◽

Yunhe Fu ◽

...

Keyword(s):

Lactic Acid ◽

Rumen Fluid ◽

Economic Loss ◽

Control Group ◽

16S Rdna Sequence ◽

16S Rdna Sequence Analysis ◽

The World ◽

Clostridium Papyrosolvens ◽

Ruminal Microbiota

Laminitis is the inflammation of the lamella, and it has caused great economic loss to the dairy industry and attracted wide attention around the world. In recent years, microbiota are considered to play a significant role in various diseases processes. Therefore, our study aimed to explore the characteristics of ruminal microbiota in laminitis cows. The serum of bovines with or without laminitis was collected to detect concentrations of lipopolysaccharide (LPS), lactic acid, and histamine, and ruminal fluid was collected for 16S rDNA sequence analysis. The results showed a significant increase in LPS and lactic acid levels in the laminitis group compared to the control group cows. In addition, a higher abundance of Candidatus Saccharimonas, Saccharofermentans, Erysipelotrichaceae UCG-009 genus, Acetobacter pasteurianus, Clostridium papyrosolvens, Ruminococcaceae bacterium AE2021, Porphyromonas crevioricanis, Pseudomonas boreopolis, Pseudomonas psychrotolerans, Rothia nasimurium, and Rothia pickettii was detected in the rumen fluid of laminitis bovines. In conclusion, this article confirms that there are differences in rumen microbiota between healthy and laminitis bovines. The elevated abundance of bacteria that enrich acid-enhancing metabolites, as well as increase the concentration of lactic acid and LPS, could be harmful factors to bovines and increase the risk of laminitis.

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Characterization of bacterial community of rumen from bovine during laminitis challenge by high-throughput sequencing

10.21203/rs.3.rs-31535/v1 ◽

2020 ◽

Author(s):

Jian Guo ◽

Haowen Sun ◽

T. Maimai ◽

Caijun Zhao ◽

Yongguo Cao ◽

...

Keyword(s):

Lactic Acid ◽

Bacterial Community ◽

High Throughput Sequencing ◽

Economic Loss ◽

Ruminal Bacteria ◽

Classic Problem ◽

Bacterial Microbiota ◽

Clostridium Papyrosolvens ◽

Acid Metabolites

Abstract Background: Laminitis is a classic problem in the dairy industry, which can cause a great economic loss. However, the etiology and pathogenesis of laminitis have yet to be understood. In recent years, the microbiota has been the focus of much investigation in the search for various diseases. The present study aimed to explore the relationship between ruminal bacterial microbiota and laminitis. Results: The serum of healthy and laminitis bovines (n=8, respectively) collected from farms was used to detect concentrations of LPS, lactic acid, and histamine by the detection kits. This study used 16S rRNA sequencing to identify the differences in the bacterial community. The results showed that there was a significant increase in LPS and lactic acid in the laminitis group. Furthermore, microbial data analysis revealed that the laminitis group increased the abundance of bacteria with acid metabolites, such as Candidatus Saccharimonas, Saccharofermentans, Erysipelotrichaceae UCG-009, and Erysipelotrichaceae UCG-008, and [Clostridium] papyrosolvens and Ruminococcaceae bacterium AE2021.Conclusions: In summary, the changes in ruminal bacteria may potentially serve as the risk of laminitis.

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Isolation, Selection, and Characterization of Lactic Acid Bacteria for a Competitive Exclusion Product To Reduce Shedding of Escherichia coli O157:H7 in Cattle

Journal of Food Protection ◽

10.4315/0362-028x-66.3.355 ◽

2003 ◽

Vol 66 (3) ◽

pp. 355-363 ◽

Cited By ~ 130

Author(s):

M. M. BRASHEARS ◽

D. JARONI ◽

J. TRIMBLE

Keyword(s):

Escherichia Coli ◽

Lactic Acid ◽

Lactic Acid Bacteria ◽

Competitive Exclusion ◽

Rumen Fluid ◽

Significant Inhibition ◽

Escherichia Coli O157 ◽

16S Rrna Sequence ◽

E Coli ◽

Acid And Bile Tolerance

Lactic acid bacteria (LAB) were selected on the basis of characteristics indicating that they would be good candidates for a competitive exclusion product (CEP) that would inhibit Escherichia coli O157:H7 in the intestinal tract of live cattle. Fecal samples from cattle that were culture negative for E. coli O157:H7 were collected. LAB were isolated from cattle feces by repeated plating on deMan Rogosa Sharpe agar and lactobacillus selection agar. Six hundred eighty-six pure colonies were isolated, and an agar spot test was used to test each isolate for its inhibition of a four-strain mixture of E. coli O157:H7. Three hundred fifty-five isolates (52%) showed significant inhibition. Seventy-five isolates showing maximum inhibition were screened for acid and bile tolerance. Most isolates were tolerant of acid at pH levels of 2, 4, 5, and 7 and at bile levels of 0.05, 0.15, and 0.3% (oxgall) and were subsequently identified with the API system. Lactobacillus acidophilus, Lactobacillus fermentum, Lactobacillus delbreukii, Lactobacillus salivarius, Lactobacillus brevis, Lactobacillus cellobiosus, Leuconostoc spp., and Pediococcus acidilactici were the most commonly identified LAB. Nineteen strains were further tested for antibiotic resistance and inhibition of E. coli O157:H7 in manure and rumen fluid. Four of these 19 strains showed susceptibility to all of the antibiotics, 13 significantly reduced E. coli counts in manure, and 15 significantly reduced E. coli counts in rumen fluid (P < 0.05) during at least one of the sampling periods. One of the strains, M35, was selected as the best candidate for a CEP. A 16S rRNA sequence analysis of M35 revealed its close homology to Lactobacillus crispatus. The CEP developed will be used in cattle-feeding trials.

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Effect of Dapagliflozin on Intestinal Flora in MafA-deficient Mice

Current Pharmaceutical Design ◽

10.2174/1381612824666180912143434 ◽

2018 ◽

Vol 24 (27) ◽

pp. 3223-3231 ◽

Cited By ~ 3

Author(s):

Luyao Li ◽

Shiyao Xu ◽

Tingting Guo ◽

Shouliang Gong ◽

Chuan Zhang

Keyword(s):

Blood Glucose ◽

High Throughput Sequencing ◽

Fatty Acid Content ◽

Fasting Blood Glucose ◽

Intestinal Flora ◽

Short Chain Fatty Acids ◽

The Body ◽

Short Chain ◽

Control Group ◽

Deficient Mice

Objective: To investigate the effect of dapagliflozin on intestinal microflora in MafA-deficient mice using an animal model of diabetes. Methods: Male MafA-deficient mice were administered dapagliflozin (1.0 mg/kg/d) intragastrically for 6 weeks. Mouse body weights and fasting blood glucose levels were measured, and intestinal short-chain fatty acids were measured by gas chromatography. A series of methods was used to analyse the number of primary harmful bacteria in the faeces, and high-throughput sequencing was used to sequence the changes in intestinal flora. Results: The weight of the mice decreased after dapagliflozin gavage, and fasting blood glucose was significantly lower than that in the control group (P < 0.001). Acetic acid and butyric acid contents in the intestinal tracts of the mice increased, and the growth of harmful microorganisms, such as Clostridium perfringens, enterococci, Enterobacteriaceae, and intestinal enterococci, was inhibited. Blautia is a species found in the experimental group and was significantly different from the control and blank groups as determined by the LDA score from highthroughput sequencing. Conclusion: Dapagliflozin can reduce fasting blood glucose, decrease body weight, increase short-chain fatty acid content, regulate the intestinal microecological balance of the body and promote blood glucose and energy homeostasis.

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Pseudomonas eucalypticola sp. nov., a producer of antifungal agents isolated from Eucalyptus dunnii leaves

Scientific Reports ◽

10.1038/s41598-021-82682-7 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Yujing Liu ◽

Zhang Song ◽

Hualong Zeng ◽

Meng Lu ◽

Weiyao Zhu ◽

...

Keyword(s):

Dna Hybridization ◽

Antifungal Agents ◽

Novel Species ◽

Phytopathogenic Fungi ◽

Strictly Aerobic ◽

Rrna Sequence ◽

16S Rrna Sequence ◽

Eucalyptus Dunnii ◽

16S Rrna Sequence Analysis

AbstractPseudomonas are ubiquitously occurring microorganisms and are known for their ability to produce antimicrobials. An endophytic bacterial strain NP-1 T, isolated from Eucalyptus dunnii leaves, exhibits antifungal properties against five tested phytopathogenic fungi. The strain is a Gram-negative rod-shaped bacterium containing a single polar flagellum. It is strictly aerobic, grows at 4–37 °C, 2–5% NaCl, and pH 3–7. The 16S rRNA sequence analysis showed that NP-1 T belongs to the Pseudomonas genus. Phylogenetic analysis based on four concatenated partial genes (16S rDNA, gyrB, rpoB and rpoD) and the phylogenomic tree indicated that NP-1 T belongs to Pseudomonas fluorescens lineage but is distinct from any known Pseudomonas species. The G + C mol % of NP-1 T genome is 63.96, and the differences between NP-1 T and related species are larger than 1. The digital DNA-DNA hybridization and tetranucleotide signatures are 23.8 and 0.97, which clearly separates strain NP-1 T from its closest neighbours, Pseudomonas coleopterorum and Pseudomonas rhizosphaerae. Its phenotypic and chemotaxonomic features confirmed its differentiation from related taxa. The results from this polyphasic approach support the classification of NP-1 T as a novel species of Pseudomonas, and the name of Pseudomonas eucalypticola is thus proposed for this strain, whose type is NP-1 T (= CCTCC M2018494T = JCM 33572 T).

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Effects of Feed Mixed with Lactic Acid Bacteria and Carbon, Nitrogen, Phosphorus Supplied to the Water on the Growth and Survival Rate of White Leg Shrimp (Penaeus vannamei) Infected with Acute Hepatopancreatic Necrosis Disease Caused by Vibrio parahaemolyticus

Biology ◽

10.3390/biology10040280 ◽

2021 ◽

Vol 10 (4) ◽

pp. 280

Author(s):

Linh Nguyen Thi Truc ◽

Tuu Nguyen Thanh ◽

To Tran Thi Hong ◽

Day Pham Van ◽

Minh Vo Thi Tuyet ◽

...

Keyword(s):

Lactic Acid ◽

Survival Rate ◽

Lactic Acid Bacteria ◽

Vibrio Parahaemolyticus ◽

Control Group ◽

Penaeus Vannamei ◽

Growth And Survival ◽

Acute Hepatopancreatic Necrosis Disease ◽

Nitrogen Phosphorus ◽

Shrimp Health

This study aimed to evaluate the growth, survival rate, and resistance to acute hepatopancreatic necrosis disease (AHPND) of white leg shrimp (Penaeus vannamei) by using Lactobacillus plantarum, Lactobacillus fermentum, and Pediococcus pentosaceus mixed with feed, and at the same time supplying CNP in a ratio of 15:1:0.1 to the water. As a result, the treatments that shrimp were fed with feed containing lactic acid bacteria (LAB), especially L. plantarum, have increased shrimp growth, total hemocyte cells, granulocyte cells, and hyaline cells significantly (p < 0.05) in comparison to the control group. The supply of CNP to the water has promoted the intensity of V. parahaemolyticus effects on shrimp health and significantly decreased total hemocyte cells, granulocyte cells, and hyaline cells by 30–50% in the period after three days of the challenge, except in L. plantarum treatment, which had only a 20% decrease compared to other treatments. In CNP supplying treatments, the AHPND infected rate and mortality of shrimp were higher than those in other treatments. In summary, the supply of CNP had significantly reduced the shrimp’s immune response and promoted the susceptibility of shrimp to AHPND in both cases of use with and without LAB-containing diets.

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Probiotic Potential of a Novel Vitamin B2-Overproducing Lactobacillus plantarum Strain, HY7715, Isolated from Kimchi

Applied Sciences ◽

10.3390/app11135765 ◽

2021 ◽

Vol 11 (13) ◽

pp. 5765

Author(s):

Joo-Yun Kim ◽

Eun-Jung Choi ◽

Jae-Ho Lee ◽

Myeong-Seok Yoo ◽

Keon Heo ◽

...

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Lactobacillus Plantarum ◽

Probiotic Strain ◽

Optimal Growth ◽

Control Group ◽

High Survival ◽

Vitamin B2 ◽

Riboflavin Deficiency

Vitamin B2, also known as riboflavin, is essential for maintaining human health. The purpose of this study was to isolate novel lactic acid bacteria that overproduce vitamin B2 and to validate their potential as probiotics. In this study, Lactobacillus plantarum HY7715 (HY7715) was selected among lactic acid bacteria isolated from Kimchi. HY7715 showed a very high riboflavin-producing ability compared to the control strain due to the high expression of ribA, ribB, ribC, ribH, and ribG genes. HY7715 produced 34.5 ± 2.41 mg/L of riboflavin for 24 h without consuming riboflavin in the medium under optimal growth conditions. It was able to produce riboflavin in an in vitro model of the intestinal environment. In addition, when riboflavin deficiency was induced in mice through nutritional restriction, higher levels of riboflavin were detected in plasma and urine in the HY7715 administration group than in the control group. HY7715 showed high survival rate in simulated gastrointestinal conditions and had antibiotic resistance below the cutoff MIC value suggested by the European Food Safety Authority; moreover, it did not cause hemolysis. In conclusion, HY7715 could be considered a beneficial probiotic strain for human and animal applications, suggesting that it could be a new alternative to address riboflavin deficiency.

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EFFECT OF SUPPLEMENTAL RATION ON CHANGES IN BLOOD COMPONENTS OF STEERS GIVEN ACCESS TO LUSH PASTURE

Canadian Journal of Animal Science ◽

10.4141/cjas71-024 ◽

1971 ◽

Vol 51 (1) ◽

pp. 179-186 ◽

Cited By ~ 1

Author(s):

E. E. LISTER ◽

W. SZEPESI ◽

K. A. WINTER ◽

H. F. JEFFERS ◽

V. S. LOGAN

Keyword(s):

Lactic Acid ◽

Rumen Fluid ◽

Plasma Calcium ◽

Lactic Acid Concentration ◽

Lower Plasma ◽

Supplemental Feed ◽

Glucose Levels ◽

Subsequent Effect ◽

Calcium And Magnesium ◽

Plasma Magnesium

Prepasture feeding of low quality hay versus hay and grain had no subsequent effect on rumen fluid ammonia, blood urea, or plasma calcium, glucose and lactic acid concentration in steers turned out on lush pasture. Steers previously fed hay and grain had lower plasma magnesium levels on the first day on unsupplemented pasture than those fed hay prior to pasture. Supplemental feed on pasture partly alleviated the depression in plasma magnesium on the first day on pasture. Feeding supplemental hay with pasture tended to lower rumen fluid ammonia, blood urea and plasma lactic acid compared with feeding no supplement during the initial days on pasture, indicating the desirability of hay feeding during this time. A hay and grain supplement with pasture had a similar effect to the hay supplement on rumen-fluid ammonia, and plasma lactic acid, but a lesser effect on blood urea concentration. Neither supplemental hay nor hay plus grain affected plasma calcium or glucose levels. All steers showed an increase in rumen fluid ammonia, blood urea and plasma lactic acid on the first day and a decrease in plasma calcium and magnesium on the second day on pasture. Blood urea, plasma magnesium and plasma calcium concentrations had not returned to prepasture levels by 21 days on pasture.

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Construction and Evaluation of a Rat Model of Postpartum Fatigue

Gynecologic and Obstetric Investigation ◽

10.1159/000517997 ◽

2021 ◽

pp. 1-9

Author(s):

Ting Bai ◽

Fan Wu ◽

Shuhan Yan ◽

Feng Zhang ◽

Xujuan Xu

Keyword(s):

Lactic Acid ◽

Rat Model ◽

Water Maze ◽

Acid Level ◽

Model Building ◽

Small Sample ◽

Control Group ◽

Lactic Acid Level ◽

Physical Fatigue ◽

Postpartum Fatigue

Objectives: The aim of the study was to construct and evaluate a rat model of postpartum fatigue. Design: This is an article about animal model building. Methods: Sprague-Dawley rats on the 1st day after delivery were randomized into control group and fatigue group. The deep sleep of rats was interfered with by forcing them to stand in water, to make the rats experience mental and physical fatigue. To maintain galactosis and lactation, rats and pups were caged for 90 min after every 3 h of separation. The control group was separated routinely without any stimulus. The model was evaluated from mental and physical fatigue on the 8th day and 15th day. The mental fatigue was evaluated by a water maze test and the rat’s 5-hydroxytryptamine (5-HT) level in hippocampus, while the physical fatigue was evaluated using lactic acid level in serum and duration of weight-loaded forced swimming. Results: Among the 7-day and 14-day modeling groups, compared with the control group, the success rate of water maze landing was significantly decreased, the time for water maze landing was significantly prolonged and 5-HT level in hippocampus significantly decreased in the fatigue group. With respect to physical fatigue, among the 7-day and 14-day modeling groups, the lactic acid level in serum in the fatigue group was significantly increased, and the duration of exhaustive swimming of rats was significantly shortened. Limitations: A small sample size was the main limitation of this study. Conclusions: We have successfully constructed a rat model of postpartum fatigue by forcing postpartum rats to stand in water, which was similar to a level of stress that contributes to the development of postpartum fatigue. Our model opens the door for future studies evaluating the effectiveness of pharmacological and behavioral therapies.

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