scholarly journals Characterization of the Bacterial Community of Rumen in Dairy Cows with Laminitis

Genes ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1996
Author(s):  
Jian Guo ◽  
Ruiying Mu ◽  
Shuang Li ◽  
Naisheng Zhang ◽  
Yunhe Fu ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Rumen Fluid ◽  
Economic Loss ◽  
Control Group ◽  
16S Rdna Sequence ◽  
16S Rdna Sequence Analysis ◽  
The World ◽  
Clostridium Papyrosolvens ◽  
Ruminal Microbiota

Laminitis is the inflammation of the lamella, and it has caused great economic loss to the dairy industry and attracted wide attention around the world. In recent years, microbiota are considered to play a significant role in various diseases processes. Therefore, our study aimed to explore the characteristics of ruminal microbiota in laminitis cows. The serum of bovines with or without laminitis was collected to detect concentrations of lipopolysaccharide (LPS), lactic acid, and histamine, and ruminal fluid was collected for 16S rDNA sequence analysis. The results showed a significant increase in LPS and lactic acid levels in the laminitis group compared to the control group cows. In addition, a higher abundance of Candidatus Saccharimonas, Saccharofermentans, Erysipelotrichaceae UCG-009 genus, Acetobacter pasteurianus, Clostridium papyrosolvens, Ruminococcaceae bacterium AE2021, Porphyromonas crevioricanis, Pseudomonas boreopolis, Pseudomonas psychrotolerans, Rothia nasimurium, and Rothia pickettii was detected in the rumen fluid of laminitis bovines. In conclusion, this article confirms that there are differences in rumen microbiota between healthy and laminitis bovines. The elevated abundance of bacteria that enrich acid-enhancing metabolites, as well as increase the concentration of lactic acid and LPS, could be harmful factors to bovines and increase the risk of laminitis.

scholarly journals Characterization of Bacterial Community of Rumen in Dairy Cows With Laminitis

2021 ◽  
Author(s):  
Jian Gao ◽  
Bo Liu ◽  
Shuang Li ◽  
Ruiying Mu ◽  
Naisheng Zhang ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Rumen Fluid ◽  
Economic Loss ◽  
Control Group ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
16S Rrna Sequence Analysis ◽  
Clostridium Papyrosolvens ◽  
Ruminal Microbiota

Abstract Background: Laminitis- an inflammation of lamella, could cause great economic loss to dairy industry, which has attracted wide attention around the world. In recent years, microbiota is considered as one of the vital parts that played significant role in various diseases processes. However, current studies are far from sufficient. Aim of this study is to explore the characteristics of ruminal microbiota in laminitis cows. Methods: The serum of bovines with or without laminitis was collected to detect concentrations of lipopolysaccharide (LPS), lactic acid, and histamine, and the ruminal fluid was collected for 16S rRNA sequence analysis. Results: The results showed that a significant increase in LPS and lactic acid levels in laminitis group comparing to control group cows. In addition, the higher abundance of bacteria that -riches acid-enhancing metabolites, namely, Candidatus Saccharimonas, Saccharofermentans, Erysipelotrichaceae UCG-009, Erysipelotrichaceae UCG-008, Clostridium papyrosolvens and Ruminococcaceae bacterium AE2021 were detected in the rumen fluid from laminitis bovines. Conclusions: This article confirmed that difference of rumen microbiota were occurred in rumen between health and laminitis bovines. The elevated abundance of bacteria that riches acid- enhancing metabolites, as well as increased the concentration of lactic acid and LPS could be harmful factors to bovines and increase risks of laminitis.

scholarly journals Characterization of bacterial community in the rumen of bovine with laminitis using high-throughput sequencing

2020 ◽  
Author(s):  
Jian Guo ◽  
Bo Liu ◽  
Tuniyazi. Maimai ◽  
Caijun Zhao ◽  
Yongguo Cao ◽  
...  
Keyword(s):  
Lactic Acid ◽  
High Throughput Sequencing ◽  
Rumen Fluid ◽  
Economic Loss ◽  
Control Group ◽  
16S Rrna Sequence ◽  
Pathological Mechanism ◽  
16S Rrna Sequence Analysis ◽  
Clostridium Papyrosolvens ◽  
Ruminal Microbiota

Abstract Background: Laminitis- an inflammation of lamella, could cause great economic loss to dairy industry, which has attracted wide attention around the world. Although previous researches explored risk factors of laminitis, the exact pathological mechanism of laminitis is still remaining unclear. In recent years, microbiota is considered as one of the vital parts that played significant role in various diseases processes. However, current studies are far from sufficient. Aim of this study is to explore the characteristics of ruminal microbiota in laminitis cows.Results: The serum of bovines with or without laminitis from farms was collected to detect concentrations of LPS, lactic acid, and histamine, and the ruminal fluid was collected for 16S rRNA sequence analysis. The results showed that there was a significant increase in LPS and lactic acid levels in laminitis group comparing to control group cows. In addition, the higher abundance of bacteria that -riches acid-enhancing metabolites, namely, Candidatus Saccharimonas, Saccharofermentans, Erysipelotrichaceae UCG-009, Erysipelotrichaceae UCG-008, Clostridium papyrosolvens and Ruminococcaceae bacterium AE2021 were detected in the rumen fluid from laminitis bovines.Conclusions: This article confirmed that difference of rumen microbiota were occurred in rumen between health and laminitis bovines. The elevated abundance of bacteria that riches acid- enhancing metabolites, as well as increased the concentration of lactic acid and LPS could be harmful factors to bovines and increase risks of laminitis.

scholarly journals Characterization of bacterial community of rumen from bovine during laminitis challenge by high-throughput sequencing

2020 ◽  
Author(s):  
Jian Guo ◽  
Haowen Sun ◽  
T. Maimai ◽  
Caijun Zhao ◽  
Yongguo Cao ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Bacterial Community ◽  
High Throughput Sequencing ◽  
Economic Loss ◽  
Ruminal Bacteria ◽  
Classic Problem ◽  
Bacterial Microbiota ◽  
Clostridium Papyrosolvens ◽  
Acid Metabolites

Abstract Background: Laminitis is a classic problem in the dairy industry, which can cause a great economic loss. However, the etiology and pathogenesis of laminitis have yet to be understood. In recent years, the microbiota has been the focus of much investigation in the search for various diseases. The present study aimed to explore the relationship between ruminal bacterial microbiota and laminitis. Results: The serum of healthy and laminitis bovines (n=8, respectively) collected from farms was used to detect concentrations of LPS, lactic acid, and histamine by the detection kits. This study used 16S rRNA sequencing to identify the differences in the bacterial community. The results showed that there was a significant increase in LPS and lactic acid in the laminitis group. Furthermore, microbial data analysis revealed that the laminitis group increased the abundance of bacteria with acid metabolites, such as Candidatus Saccharimonas, Saccharofermentans, Erysipelotrichaceae UCG-009, and Erysipelotrichaceae UCG-008, and [Clostridium] papyrosolvens and Ruminococcaceae bacterium AE2021.Conclusions: In summary, the changes in ruminal bacteria may potentially serve as the risk of laminitis.

scholarly journals Identification and characterization of lactic acid bacteria associated with tropical grass silage produced in Okinawa

2020 ◽  
Vol 8 (3) ◽  
pp. 234-249
Author(s):  
Takashi Hanagasaki
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
16S Rdna Sequence ◽  
16S Rdna Sequence Analysis ◽  
Rate Of Increase ◽  
Digitaria Eriantha ◽  
Chloris Gayana ◽  
Tropical Grass

In Okinawa, rate of increase in gross agricultural production during 2011‒2016 was the highest in Japan and sales of calves ranked fourth throughout Japan. Raising cattle by feeding high quality silage is beneficial both nutritionally and economically. However, little is known about lactic acid bacteria (LAB) present in silages made from tropical grass in Okinawa. To improve understanding of fermentation processes in silages, the LAB present in a range of silages (Digitaria eriantha, Megathyrsus maximus, Chloris gayana, Urochloa mutica, Sorghum sp. and Zea mays) were identified. All isolates were Gram-positive and mainly catalase-negative bacteria. According to morphological and biochemical characters, 37 isolates were divided into 16 groups and on the basis of 16S rDNA sequence analysis, 7 were identified as Lactobacillus plantarum, 3 as L. paraplantarum, 1 as L. brevis, 1 as L. acidipiscis, 3 as L. casei, 1 as L. fermentum, 9 as Weissella paramesenteroides, 1 as W. kimchii, 5 as Lactococcus lactis subsp. lactis, 2 as Lactococcus garvieae and 4 as Pediococcus pentosaceus. Some of this wide variety of LAB in Okinawan silage could be beneficial for improving quality of silages and further studies are planned to determine benefits of inoculating forage with particular strains at ensiling.

scholarly journals Erratum to: Deodorization of pig slurry and characterization of bacterial diversity using 16S rDNA sequence analysis

2014 ◽  
Vol 52 (12) ◽  
pp. 1056-1056
Author(s):  
Ok-Hwa Hwang ◽  
Sebastian Raveendar ◽  
Young-Ju Kim ◽  
Ji-Hun Kim ◽  
Tae-Hun Kim ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Bacterial Diversity ◽  
16S Rdna ◽  
Rdna Sequence ◽  
Pig Slurry ◽  
16S Rdna Sequence ◽  
16S Rdna Sequence Analysis

Isolation and Characterization of Laccase Activity in a Novel Bacillus amyloliquefaciens LC02

2011 ◽  
Vol 183-185 ◽  
pp. 773-777 ◽  
Author(s):  
Jun Bo Pan ◽  
Min Zhao ◽  
Lei Lu ◽  
Mei Hui Du ◽  
Guo Fu Li ◽  
...  
Keyword(s):  
Bacillus Amyloliquefaciens ◽  
Laccase Activity ◽  
Copper Ions ◽  
Bacterial Strains ◽  
Biochemical Tests ◽  
16S Rdna Sequence ◽  
16S Rdna Sequence Analysis ◽  
Isolation And Characterization ◽  
Physiological And Biochemical

Bacterial strains exhibiting laccase activity were isolated from the forest soil. A strain LC02 with syringaldazine oxidation ability was obtained using enrichment medium supplemented with copper ions. The isolated strain was identified as Bacillus amyloliquefaciens using physiological and biochemical tests as well as 16S rDNA sequence analysis. The characterization of spore laccase activity was investigated. The result showed that the optimum pH and temperature of the enzyme was 6.6 and 70°C, respectively. A great thermostability was observed for the spore laccase at 70°C. Laccase activity was strongly inhibited by 0.1 mmol/L NaN3, dithiothreitol and cysteine.

Deodorization of pig slurry and characterization of bacterial diversity using 16S rDNA sequence analysis

2014 ◽  
Vol 52 (11) ◽  
pp. 918-929 ◽  
Author(s):  
Ok-Hwa Hwang ◽  
Sebastian Raveendar ◽  
Young-Ju Kim ◽  
Ji-Hun Kim ◽  
Tae-Hun Kim ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Bacterial Diversity ◽  
16S Rdna ◽  
Rdna Sequence ◽  
Pig Slurry ◽  
16S Rdna Sequence ◽  
16S Rdna Sequence Analysis

The hunt for the G-bacteria in activated sludge biomass

1998 ◽  
Vol 37 (4-5) ◽  
pp. 65-69 ◽  
Author(s):  
A. M. Maszenan ◽  
R. J. Seviour ◽  
B. K. C. Patel ◽  
G. N. Rees ◽  
B. McDougall
Keyword(s):  
Activated Sludge ◽  
New Genus ◽  
Distinct Species ◽  
16S Rdna Sequence ◽  
Gram Negative ◽  
16S Rdna Sequence Analysis ◽  
Large Numbers ◽  
Phenotypic Characterisation ◽  
The World ◽  
Practical Implications

Routine microscopic examination of biomass samples from many activated sludge plants around the world reveals the presence of large numbers of both Gram positive and Gram negative coccoid cells often arranged in distinctive tetrads or sheets of tetrads. Three Gram negative tetrad cocci were successfully isolated into pure culture using micromanipulation from samples of biomass from plants in Tamworth, N.S.W., Australia, Verona, Italy and Macau. After extensive phenotypic characterisation and 16S rDNA sequence analysis, all isolates showed high overall similarity to each other, but were phylogenetically quite different to any previously sequenced bacterium. They have therefore been classified as three species of a new genus, the genus Amaricoccus in the α Proteobacteria in the domain Bacteria. None could accumulate polyphosphate granules, and production of intracellular poly-β-hydroxybutyrate (PHB) granules depended on the carbon source used in the medium. A similar characterisation of the original isolate of the G-bacteria described by Cech and Hartman showed this organism also belonged to the same genus as the other three isolates, but as a distinct species. The practical implications of these observations are discussed.

Screening and Characterization of a Heavy Metal Tolerance Bacterium

2012 ◽  
Vol 550-553 ◽  
pp. 1540-1544
Author(s):  
Xiao Xi Zeng ◽  
Li Yuan Chai ◽  
Jian Xin Tang ◽  
Li Jian Xu ◽  
Pei Jiang ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Sequence Analysis ◽  
Metal Tolerance ◽  
Heavy Metal Tolerance ◽  
Morphological Observation ◽  
High Tolerance ◽  
16S Rdna Sequence ◽  
16S Rdna Sequence Analysis

Strain ZSI isolated from heavy metal contaminated soil was identified by morphological observation and 16S rDNA sequence analysis. The tolerance to heavy metals and 12 antibiotics was examined. The results showed that the strain was most homophylic with Cupriavidus sp. It has high tolerance to serval metals, and the order was Cu＞Zn＞Cd＞Ni＞Mn=Co＞Pb. The strain can resistant to 9 antibiotics.

scholarly journals Isolation and Characterization of Bacillus Sp. Capable of Degradating Alkali Lignin

2021 ◽  
Vol 9 ◽  
Author(s):  
Jing Yang ◽  
Jian Zhao ◽  
Jianchun Jiang ◽  
Hao Xu ◽  
Ning Zhang ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Ligninolytic Enzyme ◽  
Enzyme Analysis ◽  
16S Rdna Sequence ◽  
Alkali Lignin ◽  
16S Rdna Sequence Analysis ◽  
Isolation And Characterization ◽  
Azure B ◽  
Physical And Chemical

Alkali lignin-degrading Bacillus were isolated from forest soils in China and were identified as Bacillus subtilis TR-03 and Bacillus cereus TR-25 by 16S rDNA sequence analysis. Wherein TR-03 displayed optimal 26.72% alkali lignin (2 g/L) degradation at 7 days and 71.23% of Azure-B (0.01%) decolorization at 36 h of cultivation at 37°C. Ligninolytic enzyme analysis revealed that TR-03 was capable of depolymerizing alkali lignin effectively by the producing of lignin peroxidase and laccase, wherein higher laccase activity was cell-associated. At last, the physical and chemical changes of lignin via SEM and FTIR analysis was further observed to prove the lignin degradation by Bacillus subtilis TR-03.

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