scholarly journals Berberine Attenuates Neonatal Sepsis in Rats By Inhibiting FOXA1 and NF-κB Signaling Transduction Via The Induction of miR-132-3p

2021 ◽  
Author(s):  
Binghui Li ◽  
Shiping Niu ◽  
Hongli Geng ◽  
Changkun Yang ◽  
Changliang Zhao
Keyword(s):  
Survival Rate ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Systemic Inflammation ◽  
Neonatal Sepsis ◽  
Molecular Level ◽  
Protective Effects ◽  
Newborn Mice ◽  
Functional Component ◽  
Tnf Α

Abstract Neonatal sepsis (NS) is a severe syndrome in newborns induced by infections, the initiation and development of which are closely related to the function of miRs. In the current study, the anti-NS effects of berberine, a functional component in Traditional Chinese medicine (TCM), were assessed by focusing on its interaction with miR-132-3p-mediated signaling. NS model was induced using cecal slurry (CS) and handled with berberine. The changes in survival rate, intestinal structure, systemic inflammation, miR-132-3p level, and activities of FOXA1 and NF-κB pathways were detected. The data showed that berberine increased survival rate of NS mice. The intestinal injures induced by CS were also attenuated by berberine, which was associated with the inhibited production of systemic IL-6, IL-1β, and TNF-α. At molecular level, the expression of miR-132-3p was up-regulated, suppressing the expressions of FOXA1, p-IκBα, and p65, while inducing the expression of IκBα. The effects of berberine on NS-induced impairments were blocked by the injection of miR-132-3p antagomir, which reduced survival rate, exacerbated intestinal injuries, induced systemic inflammation, and re-activated FOXA1 and NF-κB pathways. Collectively, the findings outlined in the current study indicated that berberine had solid protective effects on newborn mice against NS-induced symptoms, and the effects depended on the up-regulation of miR-132-3p.

scholarly journals Protective effect of Traditional Chinese medicine Shuangwu Zhentong Capsule on collagen-induced arthritis in rats and possible mechanisms

2019 ◽  
Vol 17 ◽  
pp. 205873921984340 ◽  
Author(s):  
Dandan Tian ◽  
Jianhua Sun ◽  
Jun Li ◽  
Guoqi Xie ◽  
Shaojun Hao ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Protective Effect ◽  
Synovial Tissue ◽  
Index Score ◽  
High Dose ◽  
Protective Effects ◽  
Collagen Induced Arthritis ◽  
Swelling Degree ◽  
Tnf Α

The aim of this study was to investigate the protective effect of Traditional Chinese medicine Shuangwu Zhentong Capsule (SZC) on collagen-induced arthritis (CIA) and the possible mechanisms. Sixty rats were randomly divided into control and model: low-, medium-, and high-dose SZC and prednisone acetate (PA) groups, with 10 rats in each group. The CIA model was established in later 5 groups. The rats in low-, medium-, and high-dose SZC groups were intragastricallly administered with SZC, with dose of 0.5, 1, and 2 g/kg, respectively. The PA group was intragastricallly administered with 5 mg/kg PA. The treatment was performed for 3 weeks. Before treatment and after treatment, the paw swelling degree and polyarthritis index score of rats were measured. At the end of experiment, the serum tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and nitric oxide (NO) levels were determined. The expressions of Ras and Raf-1 protein in synovial tissue were detected. Results showed that, after treatment, compared with model group, in high-dose SZC and PA groups, the paw swelling degree, polyarthritis index score, serum TNF-α, IL-1β and NO levels, and synovial tissue Ras and Raf-1 protein levels were significantly decreased, respectively ( P < 0.05). In conclusion, SZC has obvious protective effects on CIA in rats. The mechanisms may be related to its resistance of inflammatory reaction and down-regulation of Ras and Raf-l protein expressions in synovial tissue.

Mechanism of Sini Decoction on Coronary Heart Disease in Molecular Level

2013 ◽  
Vol 756-759 ◽  
pp. 2868-2872
Author(s):  
Wei Ye Tao ◽  
Lai You Wang ◽  
Guo Hua Cheng ◽  
Jun Liu ◽  
Lang Ping Tang
Keyword(s):  
Coronary Heart Disease ◽  
Heart Disease ◽  
Molecular Docking ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Small Molecules ◽  
Molecular Level ◽  
New Drugs ◽  
Curative Effect ◽  
Material Basis

Sini Decoction is a traditional Chinese medicine which has a curative effect. The mode of action between small molecules and the targets were presented visually, which provided an in-depth interpretation about the pharmacodynamic material basis. It is valuable for the research and development of new drugs. Experimental results show that we can reveal the treatment mechanism of Sini Decoction in molecular level by molecular docking.

scholarly journals Dehydrocorydaline Accounts the Majority of Anti-Inflammatory Property of Corydalis Rhizoma in Cultured Macrophage

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Xiangpeng Kong ◽  
Zhicong Chen ◽  
Yingjie Xia ◽  
Etta Y. L. Liu ◽  
Haiqin Ren ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Proinflammatory Cytokines ◽  
Blood Circulation ◽  
Nuclear Translocation ◽  
Total Alkaloids ◽  
Tnf Α ◽  
Different Types ◽  
Anti Inflammatory ◽  
Chromatographic Peaks

Corydalis Rhizoma (CR) is a commonly used traditional Chinese medicine for its potency in activating blood circulation and analgesia. In clinic, CR extracts or components are commonly used in the treatment of myocardial ischemia, rheumatism, and dysmenorrhea with different types of inflammation. However, due to different mechanism of pain and inflammation, the anti-inflammatory property of CR has not been fully revealed. Here, the major chromatographic peaks of CR extracts in different extracting solvents were identified, and the anti-inflammatory activities of CR extracts and its major alkaloids were evaluated in LPS-treated macrophages by determining expressions of proinflammatory cytokines, IκBα and NF-κB. The most abundant alkaloid in CR extract was dehydrocorydaline, having >50% of total alkaloids. Besides, the anti-inflammatory activities of dehydrocorydaline and its related analogues were demonstrated. The anti-inflammatory roles were revealed in LPS-treated cultured macrophages, including (i) inhibiting proinflammatory cytokines release, for example, TNF-α, IL-6; (ii) suppressing mRNA expressions of proinflammatory cytokines; (iii) promoting IκBα expression and suppressing activation of NF-κB transcriptional element; and (iv) reducing the nuclear translocation of NF-κB. The results supported that dehydrocorydaline was the major alkaloid in CR extract, which, together with its analogous, accounted the anti-inflammatory property of CR.

scholarly journals A Standardized Traditional Chinese Medicine Preparation Named Yejuhua Capsule Ameliorates Lipopolysaccharide-Induced Acute Lung Injury in Mice via Downregulating Toll-Like Receptor 4/Nuclear Factor-κB

2015 ◽  
Vol 2015 ◽  
pp. 1-15 ◽  
Author(s):  
Chu-Wen Li ◽  
Zhi-Wei Chen ◽  
Xiao-Li Wu ◽  
Zhao-Xiao Ning ◽  
Zu-Qing Su ◽  
...  
Keyword(s):  
Acute Lung Injury ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Lung Injury ◽  
Therapeutic Drug ◽  
Nuclear Factor ◽  
Protective Effects ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4 ◽  
Traditional Chinese Medicine Preparation

A standardized traditional Chinese medicine preparation named Yejuhua capsule (YJH) has been clinically used in treatments of various acute respiratory system diseases with high efficacy and low toxicity. In this study, we were aiming to evaluate potential effects and to elucidate underlying mechanisms of YJH against lipopolysaccharide- (LPS-) induced acute lung injury (ALI) in mice. Moreover, the chemical analysis and chromatographic fingerprint study were performed for quality evaluation and control of this drug. ALI was induced by intratracheal instillation of LPS (5 mg/kg) into the lung in mice and dexamethasone (5 mg/kg, p.o.) was used as a positive control drug. Results demonstrated that pretreatments with YJH (85, 170, and 340 mg/kg, p.o.) effectively abated LPS-induced histopathologic changes, attenuated the vascular permeability enhancement and edema, inhibited inflammatory cells migrations and protein leakages, suppressed the ability of myeloperoxidase, declined proinflammatory cytokines productions, and downregulated activations of nuclear factor-κB (NF-κB) and expressions of toll-like receptor 4 (TLR4). This study demonstrated that YJH exerted potential protective effects against LPS-induced ALI in mice and supported that YJH was a potential therapeutic drug for ALI in clinic. And its mechanisms were at least partially associated with downregulations of TLR4/NF-κB pathways.

Protective effects of a Traditional Chinese Medicine, You-Gui-Wan, on steroid-induced inhibition of cytokine production in mice

2005 ◽  
Vol 5 (6) ◽  
pp. 1041-1048 ◽  
Author(s):  
Chengfang Yao ◽  
Li Wang ◽  
Shengye Cai ◽  
Haiming Wei ◽  
Xianbin Zhou ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Cytokine Production ◽  
Protective Effects
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