scholarly journals Study of arsenic genotoxicity in a freshwater fish (Channa punctatus) using RAPD as molecular marker

2021 ◽  
Author(s):  
Deepak K Jha ◽  
Niti Yashvardhini ◽  
Saurav Bhattacharya ◽  
Kumar Sayrav ◽  
Amod Kumar ◽  
...  
Keyword(s):  
Molecular Marker ◽  
Freshwater Fish ◽  
Channa Punctatus ◽  
Randomly Amplified Polymorphic Dna ◽  
Arbitrary Primers ◽  
Banding Patterns ◽  
Group Band ◽  
Before And After ◽  
Global Concern ◽  
Polymerase Chain

Abstract Arsenic, the proven genotoxic carcinogen in humans, is a groundwater contaminant of global concern. The present work investigates the applicability of randomly amplified polymorphic DNA (RAPD) as molecular marker to demonstrate arsenic genotoxicity in the freshwater fish, Channa punctatus. Fish specimens, segregated into three groups, were exposed to 10, 50 and 500 µgL− 1 of arsenic respectively for 20 consecutive days. DNA extracted from same specimens of each group before and after exposure was amplified by polymerase chain reaction (PCR) using single arbitrary primers. Marked changes in RAPD profiles of fish DNA were observed upon exposure to arsenic compare to RAPD profiles of their pre-exposure state, resulting from loss or gain of certain bands. Altogether a total of 41 loci were amplified with 37–41 bands in each group. Band 1 of pre-exposure state was lost in all post-exposure samples while bands 4, 6 and 7 appeared as new bands after exposure to arsenic. The changes in the RAPD banding patterns upon exposure to arsenic reflect alterations in fish DNA. The RAPD bands, therefore, appeared as potential markers, capable of revealing the genotoxicity induced by arsenic in this piscine model.

Genetic diversity amongst Australian canola cultivars determined by randomly amplified polymorphic DNA

1997 ◽  
Vol 37 (7) ◽  
pp. 793 ◽  
Author(s):  
R. J. Mailer ◽  
N. Wratten ◽  
M. Vonarx
Keyword(s):  
Cultivar Identification ◽  
Genetic Material ◽  
Randomly Amplified Polymorphic Dna ◽  
Chain Reaction ◽  
Arbitrary Primers ◽  
Breeding Programs ◽  
Accurate Picture ◽  
The Common ◽  
Polymerase Chain ◽  
Selection Of

Summary. The rapid increase in canola production in Australia in recent years has been associated with the release of several new cultivars with progressive improvements in quality, yield and disease resistance. The origin of many Australian cultivars is Canadian material that was introduced into Australia in the late 1970s. A selection of new genetic material has recently been introduced from diverse sources including China, Japan and Europe. As a result, Australian cultivars now contain a much larger gene pool and wide ranging agronomic characteristics. Cultivar identification and discrimination has become an important issue in canola breeding programs. This paper describes the diversity amongst 25 Australian cultivars, analysed using the polymerase chain reaction and arbitrary primers to detect randomly amplified polymorphic DNA (RAPD) sequences. From 19 primers, 89 bands were detected. Six of the best of these primers, producing 27 bands, were selected for cultivar discrimination. Cluster analysis was used to illustrate the diversity of genetic material in Australian canola cultivars and between individual breeding programs. Despite the common origin of Australian cultivars, differences between individual programs are evident. The results obtained are generally in agreement with known pedigrees. However, RAPD provides canola breeders with a more accurate picture of similarities and diversity among cultivars. The similarity of cultivars within individual programs suggests RAPD discrimination may be associated with particular cultivar characteristics and able to be used in selections in breeding programs.

Pim-1 is up-regulated by constitutively activated FLT3 and plays a role in FLT3-mediated cell survival

Blood ◽  
2005 ◽  
Vol 105 (4) ◽  
pp. 1759-1767 ◽  
Author(s):  
Kyu-Tae Kim ◽  
Kristin Baird ◽  
Joon-Young Ahn ◽  
Paul Meltzer ◽  
Michael Lilly ◽  
...  
Keyword(s):  
Tyrosine Kinase ◽  
Quantitative Polymerase Chain Reaction ◽  
Colony Growth ◽  
Dominant Negative ◽  
Internal Tandem Duplication ◽  
Downstream Target ◽  
Before And After ◽  
Flt3 Expression ◽  
Polymerase Chain ◽  
Expression Microarrays

AbstractConstitutively activating internal tandem duplication (ITD) mutations of the receptor tyrosine kinase FLT3 (Fms-like tyrosine kinase 3) play an important role in leukemogenesis, and their presence is associated with poor prognosis in acute myeloid leukemia (AML). To better understand FLT3 signaling in leukemogenesis, we have examined the changes in gene expression induced by FLT3/ITD or constitutively activated wild-type FLT3 expression. Microarrays were used with RNA harvested before and after inhibition of FLT3 signaling. Pim-1 was found to be one of the most significantly down-regulated genes upon FLT3 inhibition. Pim-1 is a proto-oncogene and is known to be up-regulated by signal transducer and activator of transcription 5 (STAT5), which itself is a downstream target of FLT3 signaling. Quantitative polymerase chain reaction (QPCR) confirmed the microarray results and demonstrated approximately 10-fold decreases in Pim-1 expression in response to FLT3 inhibition. Pim-1 protein also decreased rapidly in parallel with decreasing autophosphorylation activity of FLT3. Enforced expression of either the 44-kDa or 33-kDa Pim-1 isotypes resulted in increased resistance to FLT3 inhibition-mediated cytotoxicity and apoptosis. In contrast, expression of a dominant-negative Pim-1 construct accelerated cytotoxicity in response to FLT3 inhibition and inhibited colony growth of FLT3/ITD-transformed BaF3 cells. These findings demonstrate that constitutively activated FLT3 signaling up-regulates Pim-1 expression in leukemia cells. This up-regulation contributes to the proliferative and antiapoptotic pathways induced by FLT3 signaling. (Blood. 2005;105: 1759-1767)

Polymerase chain reaction based mapping of rye involving repeated DNA sequences

Genome ◽  
1992 ◽  
Vol 35 (4) ◽  
pp. 621-626 ◽  
Author(s):  
Peter M. Rogowsky ◽  
Ken W. Shepherd ◽  
Peter Langridge
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Sequences ◽  
Chromosome 1 ◽  
Repeated Dna ◽  
Chain Reaction ◽  
Pcr Marker ◽  
Banding Patterns ◽  
Repeated Dna Sequences ◽  
Cereal Rye ◽  
Polymerase Chain

A novel type of polymerase chain reaction (PCR) marker was developed for the mapping of cereal rye (Secale cereale). Primer pairs were synthesized targeting the insertion sites of three individual copies of the R173 family of rye specific repeated DNA sequences. While one primer was derived from a sequence within the respective R173 element, the second primer corresponded to a flanking region. The complex banding patterns obtained in rye allowed not only the mapping of the three R173 elements to certain chromosome regions of 1RS (the short arm of rye chromosome 1) but also the mapping of an additional 3–10 easily identifiable bands per primer pair to other rye chromosomes. Linkage mapping of a polymorphic 1R band derived from three rye cultivars demonstrated the presence of nonallelic, dominant markers in two independent crosses. Because of the high copy number of the R173 family (15 000 copies per diploid rye genome), its dispersion over the entire length of all chromosomes and the high number of markers obtained per primer pair, PCR markers based on the R173 family provide an almost unlimited source for well-spaced markers in rye mapping.Key words: polymerase chain reaction, mapping, repetitive DNA sequences, wheat, rye.

Genetic diversity and phylogeny analysis of Azolla based on DNA amplification by arbitrary primers

Genome ◽  
1993 ◽  
Vol 36 (4) ◽  
pp. 686-693 ◽  
Author(s):  
Benoit Van Coppenolle ◽  
Iwao Watanabe ◽  
Charles Van Hove ◽  
Gerard Second ◽  
Ning Huang ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Polymerase Chain Reaction ◽  
Principal Component ◽  
Dna Amplification ◽  
Component Analysis ◽  
Genetic Distances ◽  
Group Method ◽  
Chain Reaction ◽  
Arbitrary Primers ◽  
Polymerase Chain

The polymerase chain reaction was used to amplify random sequences of DNA from 25 accessions of Azolla to evaluate the usefulness of this technique for identification and phylogenetic analysis of this aquatic fern. Accessions were selected to represent all known species within the genus Azolla and to encompass the worldwide distribution of the fern. Primers of 10 nucleotides with 70% G + C content were used to generate randomly amplified polymorphic DNA from the symbiotic Azolla–Anabaena complex. Twenty-two primers were used and each primer gave 4–10 bands of different molecular weights for each accession. Bands were scored as present or absent for each accession and variation among accessions was quantified using Nei's genetic distances. A dendrogram summarizing phenetic relationships among the 25 accessions was generated using the unweighted pair-group method with arithmetic mean. Principal component analysis was also used to evaluate genetic similarities. Three distinct groups were identified: group 1 contains five species, group 2 contains the pinnata species, and group 3 contains the nilotica species. The analysis demonstrates that the major groups of Azolla species can be easily distinguished from one an other and, in addition, that closely related accessions within species can be identified. We further found that using 10 primers, a phylogeny that is essentially the same as that derived from 22 primers can be constructed. Our results suggest that total DNA extracted from the Azolla–Anabaena symbionts is useful for classification and phylogenetic studies of Azolla.Key words: Azolla–Anabaena symbiosis, genetic distances, polymerase chain reaction, principal component analysis.

scholarly journals Molecular typing of bacteria of the genus Asaia in malaria vector Anopheles arabiensis Patton, 1905

2012 ◽  
Vol 44 (2) ◽  
pp. 7 ◽  
Author(s):  
S. Epis ◽  
M. Montagna ◽  
F. Comandatore ◽  
C. Damiani ◽  
A. Diabaté ◽  
...  
Keyword(s):  
Molecular Typing ◽  
Internal Transcribed Spacer ◽  
Anopheles Arabiensis ◽  
Acetic Acid Bacterium ◽  
Sub Saharan Africa ◽  
Randomly Amplified Polymorphic Dna ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Sub Saharan ◽  
Dna Pcr

The acetic acid bacterium <em>Asaia</em> spp. was successfully detected in <em>Anopheles arabiensis</em> Patton, 1905, one of the major vector of human malaria in Sub-Saharan Africa. A collection of 45 <em>Asaia</em> isolates in cellfree media was established from 20 individuals collected from the field in Burkina Faso. 16S rRNA universal polymerase chain reaction (PCR) and specific qPCR, for the detection of <em>Asaia</em> spp. were performed in order to reveal the presence of different bacterial taxa associated with this insect. The isolates were typed by internal transcribed spacer-PCR, BOX-PCR, and randomly amplified polymorphic DNA-PCR, proved the presence of different <em>Asaia</em> in <em>A. arabiensis</em>.

scholarly journals Use of Public Transport and Contraction of SARS-CoV-2 in a Large Prospective Cohort in Norway.

2021 ◽  
Author(s):  
Merete Ellingjord-Dale ◽  
Karl Trygve Kalleberg ◽  
Mette Istre ◽  
Anders B. Nygaard ◽  
Sonja H. Brunvoll ◽  
...  
Keyword(s):  
Public Transport ◽  
Public Transportation ◽  
Polymerase Chain Reaction Test ◽  
Calendar Time ◽  
Frequency Of Use ◽  
Before And After ◽  
Polymerase Chain ◽  
Chain Reaction Test ◽  
Incident Cases

Abstract Background: For many people public transport is the only mode of travel, and it can be challenging to keep the necessary distances in such a restricted space. The exact role of public transportation and risk of SARS-CoV-2 transmission is not known. Methods: Participants (n=121 374) were untested adult Norwegian residents recruited through social media who in the spring of 2020 completed a baseline questionnaire on demographics and use of public transport. Incident cases (n=1069) had a positive SARS-CoV-2 polymerase chain reaction test registered at the Norwegian Messaging System for Infectious Diseases by January 27, 2021. We investigated the association between use of public transport and SARS-CoV-2 using logistic regression. Odds ratios (ORs) with 95% confidence intervals (CIs) adjusted for age, calendar time, gender, municipality, smoking, income level, fitness and underlying medical conditions were estimated. Frequency of use of public transport was reported for 2 week-periods. Results: Before lockdown, those who tested positive on SARS-CoV-2 were more likely to have used public transport 1-3 times (OR =1.28, CI 1.09-1.51), 4-10 times (OR=1.49, CI 1.26-1.77) and ≥11 times (OR=1.50, CI 1.27-1.78, p for trend<0.0001) than those who had not tested positive. Conclusion: Use of public transport was positively associated with contracting SARS-CoV-2 both before and after lockdown.

scholarly journals Incidence of COVID-19 Infection in Hospital Workers From March 1, 2020 to May 31, 2021 Routinely Tested, Before and After Vaccination With BNT162B2

2021 ◽  
Author(s):  
Francesca Larese Filon ◽  
Francesca Rui ◽  
Federico Ronchese ◽  
Paola Michieli ◽  
Corrado Negro
Keyword(s):  
Health Care ◽  
Health Care Workers ◽  
Hospital Workers ◽  
Factors Associated ◽  
Care Workers ◽  
Before And After ◽  
Polymerase Chain ◽  
Incident Rate ◽  
Rate Ratios ◽  
Monthly Incidence

Abstract Objective To evaluate the incidence of COVID-19 infection in health care workers from the start of COVID-19 pandemic in NE of Italy, to the vaccination with BNT162b2. Materials and methods This was a retrospective cohort study. Health care workers were routinely tested for SARS-CoV-2 infections using real-time polymerase chain reaction tests in nasopharyngeal swabs. Logistic regression was used to calculate incident rate ratios (IRRs) of factors associated to COVID-19. Results A total of 4251 workers were followed-up and an annual incidence of COVID-19 of 13.6% was found. In March 2021 the incidence of infection was 4.88 and 103.55 cases for 100.000 person-days in vaccinated and non-vaccinated workers, respectively, with an adjusted IRRs of 0.05 (95% CI 0.02–0.08). Conclusions Our study evaluated the monthly incidence in health care workers in Trieste hospitals before and after the vaccination finding the protective effect of BNT162B2 vaccine in 95% of health care workers routinely tested.

scholarly journals Effects of Aging Torque Controllers on Screw Tightening Force and Bacterial Micro-Leakage on the Implant-Abutment Complex

Materials ◽  
2022 ◽  
Vol 15 (2) ◽  
pp. 620
Author(s):  
Yousef Jiries ◽  
Tamar Brosh ◽  
Shlomo Matalon ◽  
Vladimir Perlis ◽  
Zeev Ormianer
Keyword(s):  
Polymerase Chain Reaction Test ◽  
Tightening Force ◽  
Reduction In Force ◽  
Implant Abutment ◽  
Before And After ◽  
Spring Type ◽  
Polymerase Chain ◽  
Bacterial Leakage ◽  
Effects Of Aging ◽  
Chain Reaction Test

Aim: We assess the accuracy of torque controllers after several aging processes and the bacterial leakage on Implant-Abutment complexes (IAC).Methods: A total of 12 spring-type and 12 friction-type torque controllers and 48 IAC (24 conical and 24 hexagonal connections) were evaluated. Chemical, mechanical, temperature, and pressure-aging methods were applied individually to replicate clinical use. Torque controller accuracy was analyzed before and after aging using a calibrated gauge. To assess bacterial leakage, the IAC were suspended in a bacterial medium for 24 h. Direct Contact Test (DCT) and Polymerase Chain Reaction Test (RT-PCR) analyzed the infiltration of F. nucleatum and P. gingivalis into the IAC micro-gap. Results: A significant decrease in torque after 10 days of aging was found. The spring-type torque controller was affected the most, regardless of the aging method (P < 0.05). PCR results indicated that all groups exhibited significantly more bacterial leakage, regardless of the method used (P < 0.05). The conical IAC demonstrated more bacterial leakage of P. gingivalis compared with the hexagonal IAC (P = 0.07). DCT found bacterial growth in the IAC only before aging and was not identified after aging. Conclusion: Aging affects torque accuracy. A reduction in force was noticed after 10 days. The conical IAC exhibits more bacterial leakage, although this was not statistically significant.

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