scholarly journals Serum miRNA Profile in Diabetic Patients with Ischemic Heart Disease as a Promising non-invasive Biomarker

2020 ◽  
Author(s):  
Agnieszka Bielska ◽  
Witold Bauer ◽  
Anna Szalkowska ◽  
Iwona Sidorkiewicz ◽  
Anna Skwarska ◽  
...  
Keyword(s):  
Heart Disease ◽  
Ischemic Heart Disease ◽  
Target Genes ◽  
Diagnostic Value ◽  
Functional Enrichment ◽  
Ischemic Heart ◽  
Individual Risk ◽  
Diabetic Patients ◽  
Serum Samples ◽  
Potential Biomarker

Abstract Background The increasing morbidity and mortality of type 2 diabetic mellitus (T2DM) patients with ischemic heart disease (IHD) highlights an urgent need to identify early biomarkers, which would help to predict individual risk of development of IHD. Here, we postulate that circulating serum-derived miRNAs may serve as potential biomarkers for early IHD diagnosis and help to identify diabetic individuals with a predisposition to undergo IHD. Methods We obtained serum samples from T2DM patients either with IHD or IHD-free and analysed the expression levels of 798 miRNAs using the NanoString nCounter Technology Platform. The prediction of the putative miRNAs targets was performed using the Ingenuity Pathway Analysis (IPA) software. Receiver operating characteristic (ROC) analysis was used to assess the diagnostic value of identified miRNAs. Results Our data showed that 9 miRNAs (miR-1224-5p, miR-1303, miR-3147, miR-4455, miR-498, miR-548b-3p, miR-548d-3p, miR-615-3p, miR-651-5p) were significantly upregulated in T2DM IHD patients compared to T2DM patients without IHD. In patients with upregulated miRNA, functional enrichment analysis of target genes by IPA indicated networks and canonical pathways involved in the pathology of the cardiovascular system. All tested miRNAs showed high diagnostic value (AUC > 0.8). Conclusions Taken together, our findings suggest that circulating miRNAs might have a crucial role in the development of IHD in diabetic patients and may be used as a potential biomarker for early diagnosis.

Serum Lipids Profiling Perturbances in Patients with Ischemic Heart Disease and Ischemic Cardiomyopathy

2020 ◽  
Author(s):  
Lin Yang ◽  
Liang Wang ◽  
Yangyang Deng ◽  
Lizhe Sun ◽  
Bowen Low ◽  
...  
Keyword(s):  
Heart Disease ◽  
Ischemic Heart Disease ◽  
Ischemic Cardiomyopathy ◽  
Serum Lipids ◽  
Ischemic Heart ◽  
Negative Ion ◽  
Serum Samples ◽  
Cross Sectional ◽  
Negative Ion Mode ◽  
Positive Ion

Abstract Background: Ischemic heart disease (IHD) is a common cardiovascular disorder associated with inadequate blood supply to the myocardium. Chronic coronary ischemia leads to ischemic cardiomyopathy (ICM). Despite their rising prevalence and morbidity, few studies have discussed the lipids alterations in these patients. Methods: In this cross-sectional study, we analyzed serum lipids profile in IHD and ICM patients using a lipidomics approach. Consecutive consenting patients admitted to the hospital for IHD and ICM were enrolled. Serum samples were obtained after overnight fasting. Non-targeted metabolomics was applied to demonstrate lipids metabolic profile in control, IHD and ICM patients. Results: A total of 63 and 62 lipids were detected in negative and positive ion mode respectively. Among them, 16:0 Lyso PI, 18:1 Lyso PI in negative ion mode, and 19:0 Lyso PC, 12:0 SM d18:1/12:0, 15:0 Lyso PC, 17:0 PC, 18:1-18:0 PC in positive ion mode were significantly altered both in IHD and ICM as compared to control. 13:0 Lyso PI, 18:0 Lyso PI, 16:0 PE, 14:0 PC DMPC, 16:0 ceramide, 18:0 ceramide in negative ion mode, and 17:0 PE, 19:0 PC, 14:0 Lyso PC, 20:0 Lyso PC, 18:0 PC DSPC, 18:0-22:6 PC in positive ion mode were significantly altered only in ICM as compared to IHD and control. Conclusion: Using non-targeted lipidomics profiling, we have successfully identified a group of circulating lipids that were significantly altered in IHD and ICM. The lipids metabolic signatures shed light on potential new biomarkers and therapeutics for preventing and treating ICM.

scholarly journals Prediction of Ischemic Heart Disease and Stroke in Survivors of Childhood Cancer

2018 ◽  
Vol 36 (1) ◽  
pp. 44-52 ◽  
Author(s):  
Eric J. Chow ◽  
Yan Chen ◽  
Melissa M. Hudson ◽  
Elizabeth A.M. Feijen ◽  
Leontien C. Kremer ◽  
...  
Keyword(s):  
Heart Disease ◽  
High Risk ◽  
Ischemic Heart Disease ◽  
Childhood Cancer ◽  
Area Under The Curve ◽  
Risk Groups ◽  
Ischemic Heart ◽  
Risk Scores ◽  
Individual Risk ◽  
Survivors Of Childhood Cancer

Purpose We aimed to predict individual risk of ischemic heart disease and stroke in 5-year survivors of childhood cancer. Patients and Methods Participants in the Childhood Cancer Survivor Study (CCSS; n = 13,060) were observed through age 50 years for the development of ischemic heart disease and stroke. Siblings (n = 4,023) established the baseline population risk. Piecewise exponential models with backward selection estimated the relationships between potential predictors and each outcome. The St Jude Lifetime Cohort Study (n = 1,842) and the Emma Children’s Hospital cohort (n = 1,362) were used to validate the CCSS models. Results Ischemic heart disease and stroke occurred in 265 and 295 CCSS participants, respectively. Risk scores based on a standard prediction model that included sex, chemotherapy, and radiotherapy (cranial, neck, and chest) exposures achieved an area under the curve and concordance statistic of 0.70 and 0.70 for ischemic heart disease and 0.63 and 0.66 for stroke, respectively. Validation cohort area under the curve and concordance statistics ranged from 0.66 to 0.67 for ischemic heart disease and 0.68 to 0.72 for stroke. Risk scores were collapsed to form statistically distinct low-, moderate-, and high-risk groups. The cumulative incidences at age 50 years among CCSS low-risk groups were < 5%, compared with approximately 20% for high-risk groups ( P < .001); cumulative incidence was only 1% for siblings ( P < .001 v low-risk survivors). Conclusion Information available to clinicians soon after completion of childhood cancer therapy can predict individual risk for subsequent ischemic heart disease and stroke with reasonable accuracy and discrimination through age 50 years. These models provide a framework on which to base future screening strategies and interventions.

scholarly journals COMPARATIVE STUDY OF LEVELS OF SELECTIVE OXIDATIVE STRESS MARKERS (MALONDIALDEHYDE, ZINC, AND ANTIOXIDANT VITAMINS A, E, AND C) IN ISCHEMIC AND NON-ISCHEMIC HEART DISEASE PATIENTS SUFFERING FROM TYPE- 2 DIABETES

2018 ◽  
Vol 11 (8) ◽  
pp. 508
Author(s):  
Abd Elgadir A Altoum ◽  
Ahmed L Osman ◽  
Asaad Ma Babker
Keyword(s):  
Oxidative Stress ◽  
Heart Disease ◽  
Vitamin A ◽  
Ischemic Heart Disease ◽  
Serum Vitamin ◽  
Antioxidant Vitamins ◽  
Ischemic Heart ◽  
Diabetic Patients ◽  
Oxidative Stress Markers ◽  
Stress Markers

Objective: The objective of the current study is to compare the levels of oxidative stress markers malondialdehyde (MDA), zinc, and antioxidant Vitamins (A, E, and C) in ischemic heart disease (IHD) and non-IHD patients with diabetes mellitus.Method: This is cross-sectional study group, conducted in the advanced diagnostic center (Khartoum- Sudan) from the period May 2013 to August 2015. Comprised 100 healthy subjects were, control group with mean (fasting blood sugar) 5.61=m mol/L, the age ranged from 22 to 78 years old, the average of the age was 50.1 years. 300 patients (78 IHD and 222 without) as cases groups, the ages ranged from 30 to 80 years. The age average was 51.2 years, all samples were in a state of fasting for 12 h, and the data were collected using a structured questionnaire and direct interview to collect information. Blood specimens were collected from both groups, and plasma levels of MDA, zinc, and antioxidant Vitamins (A, E, and C) were determined.Results: There was a significant difference between the level of serum Vitamin A, E, and MDA in diabetic patients with IHD and those diabetic without IHD (p<0.05). No significant differences in serum Vitamin C level between both groups (p>0.05).Conclusion: Due to the significant differences in serum Vitamin A, E, Zinc, and MDA between diabetics with IHD and diabetics without IHD, these parameters can be used as prognostic markers for prediction of oxidative stress and antioxidant stress of diabetic patients with IHD complications.

scholarly journals Diagnostic value of exercise thallium-201 scintigraphy for ischemic heart disease in patients with chronic renal failure.

1991 ◽  
Vol 24 (11) ◽  
pp. 1453-1461
Author(s):  
Shigeaki Sato ◽  
Makoto Ohta ◽  
Michimasa Soejima ◽  
Hiroshi Tanaka ◽  
Kenichi Sugimoto ◽  
...  
Keyword(s):  
Renal Failure ◽  
Heart Disease ◽  
Chronic Renal Failure ◽  
Ischemic Heart Disease ◽  
Diagnostic Value ◽  
Thallium 201 ◽  
Ischemic Heart ◽  
Thallium 201 Scintigraphy

scholarly journals Comparative Study of Triglyceride Level in Ischemic Heart Disease Patients in different age groups of Patients in south east Rajasthan

2018 ◽  
Vol 6 (2) ◽  
pp. 92-99
Author(s):  
Darab Singh Underwal ◽  
Sushma Pandey ◽  
Deepak Gupta
Keyword(s):  
Heart Disease ◽  
Ischemic Heart Disease ◽  
Triglyceride Level ◽  
Colorimetric Method ◽  
Serum Triglyceride ◽  
Ischemic Heart ◽  
Diabetic Patients ◽  
Serum Triglyceride Level ◽  
Elderly Male ◽  
Male Patients

Backgrounds: Elevated body triglycerides have been implicated as a risk factor of ischemic heart disease (IHD).Objectives: To study the association of serum triglyceride with ischemic heart disease and to assess the relationship of serum triglyceride with other established conventional risk factors.Methods: A cross sectional case-control study of 75 cases of IHD and 75 controls without having any evidence of IHD/CHD between age group 30-70 years. Serum triglyceride levels were estimated by using Colorimetric Method and other risk factors by enzymatic methods.Results: Mean serum triglyceride (263.674 ± 89.029mg/dl) was significantly higher in cases than controls (98.833 ± 62.682mg/dl). Amongst the patients of IHD, significantly higher level of Serum triglyceride was found in diabetics (340.63±90.78mg/dl) than non-diabetics (225.19±58.30mg/dl), male elderly (>60years of age) smokers (304.20±88.60mg/dl) compared to non smoker (206.37±48.88mg/dl), elderly male with high (>150mg/dl) LDL (323.48±86.73mg/dl) compared to patients with normal (<150mg/dl) LDL (249.33±66.12 mg/dl). Similarly male patients of IHD with high (>40mg/dl) VLDL had significantly higher serum triglyceride (326.49±77.95mg/dl) compared to male patients with normal (<40mg/dl) VLDL (257.18±85.46mg/dl).Conclusion: High serum triglyceride level (>200mg/dl) may provide a cost effective tool for predicting an impending ischemic heart disease especially in diabetic patients, male elderly smokers, elderly males with high LDL, male patients of ischemic heart disease with high VLDL level.

scholarly journals The Risk of Developing Ischemic Stroke in Patients After Exacerbation of Ischemic Heart Disease

Kardiologiia ◽  
2020 ◽  
Vol 60 (8) ◽  
pp. 46-53
Author(s):  
V. A. Brazhnik ◽  
L. O. Minushkina ◽  
N. R. Khasanov ◽  
E. D. Kosmacheva ◽  
M. A. Chichkova ◽  
...  
Keyword(s):  
Heart Disease ◽  
Ischemic Stroke ◽  
Ischemic Heart Disease ◽  
Roc Curve ◽  
Prognostic Model ◽  
External Validation ◽  
Diagnostic Value ◽  
Ischemic Heart ◽  
Blood Pressure Goal ◽  
Coronary Syndrome

Aim      To develop a model for evaluating the risk of stroke in patients after exacerbation of ischemic heart disease who were admitted to the hospitals included into a vascular program.Materials and methods This study included 1803 patients with acute coronary syndrome (ACS) from four institutions of Moscow, Kazan, Astrakhan, and Krasnodar where the vascular program was established. Mean age of patients was 64.9±12.78 years, 62,1 % of them were men. The patients were followed up for one year after the discharge from the hospital. External validation of the developed prognostic model was performed on a cohort of patients with ACS included into the RECORD-3 study.Results During the follow-up period, 42 cases of ischemic stroke were observed. The risk of ischemic stroke was associated with the presence of atrial fibrillation (odd ratio (OR) 2.640; р=0.037), diabetes mellitus (OR 2.718; р=0.041), and chronic heart failure (OR 7.049; р=0.011). Protective factors were high-density lipoprotein cholesterol >1 mmol/l (OR 0.629; р=0.041), percutaneous coronary intervention during an index hospitalization (OR 0.412; р=0.042), anticoagulant treatment (OR 0.670; р=0.049), and achieving the blood pressure goal (OR 0.604; р=0.023). The prognostic model developed on the basis of regression analysis showed a good predictive value (area under the ROC curve, 0.780), sensitivity of 80 %, and specificity of 64.6 %. The diagnostic value of other scales for risk assessment was somewhat lower. The area under the ROC curve was 0.692±0.0245 for the GRACE scale and 0.708±0.0334 for CHA2DS2‑VASc. In the external validation of the scale based on data of the RECORD-3 study, the diagnostic value was lower although satisfactory as well (area under the ROC curve, 0.651); sensitivity was 78.9 %, and specificity was 52.3 %.Conclusion      The study resulted in development of a simple clinical scale, which will probably allow identifying groups at risk of stroke more precisely than with standard scales. 

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