scholarly journals Isolation and Characterization of the Mycorrhiza Helper Bacteria from the Fairy Rings of Floccularia Luteovirens

2020 ◽  
Author(s):  
minghang xu ◽  
Rui Xing ◽  
Qing-bo Gao ◽  
Fa-qi Zhang ◽  
Shi-long Chen
Keyword(s):  
Fungal Growth ◽  
Ectomycorrhizal Fungus ◽  
Tibet Plateau ◽  
Mycorrhizal Symbiosis ◽  
Bacterial Isolates ◽  
Mycorrhiza Helper Bacteria ◽  
Isolation And Characterization ◽  
Fairy Rings ◽  
Qinghai Tibet Plateau

Abstract Floccularia luteovirens is an edible ectomycorrhizal fungus which forms fairy rings (FRs) distributed on the Qinghai-Tibet Plateau. Our previous study found four isolates of potential mycorrhiza helper bacteria (MHB), but they were only isolated from a single FR and their mycorrhizal promoting ability was not tested. Thus, it was necessary to collect samples from a larger area and measure the mycorrhizal promoting ability of the potential candidates. Of 184 bacterial isolates from five fairy rings located in Qinghai-Tibet Plateau belonging to 12 species, and 7 of them (58.3%) significantly promoting the growth of F. luteovirens. We also tested the symbiosis-promoting ability of the isolates, and the results showed that only four of them stimulated the formation of mycorrhizal symbiosis. This is the first report of the construction of mycorrhizal symbiosis between F. luteovirens and its host plant in greenhouse conditions. Our results showed that the strain A89 (Achromobacter marplatensis) could produce antifungal properties but nonetheless stimulated the fungal growth. This may due to the nutrient condition of medium and the genotype specificity of MHBs. The results suggest that the promotion of significant growth is not a good predictor for mycorrhiza formation promotion ability of MHB.

scholarly journals Vector-Borne Pathogens with Veterinary and Public Health Significance in Melophagus ovinus (Sheep Ked) from the Qinghai-Tibet Plateau

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 249
Author(s):  
Qing-Xun Zhang ◽  
Ye Wang ◽  
Ying Li ◽  
Shu-Yi Han ◽  
Bo Wang ◽  
...  
Keyword(s):  
Public Health ◽  
Molecular Characterization ◽  
Tibet Plateau ◽  
Border Disease Virus ◽  
Public Health Significance ◽  
Melophagus Ovinus ◽  
Health Significance ◽  
Vector Borne ◽  
Qinghai Tibet Plateau

Melophagus ovinus (sheep ked) is a hematophagous ectoparasite that mainly parasitizes sheep. In addition to causing inflammation, wool loss, and skin damage to the animal hosts, M. ovinus also serves as a vector for a variety of pathogens and is highly likely to participate in the life and transmission cycle of pathogenic organisms. Herein, we investigated the presence and molecular characterization of vector-borne pathogens in M. ovinus from Qinghai-Tibet Plateau, China. A total of 92 M. ovinus pools collected from the Qinghai province of China were screened for the presence of selected vector-borne pathogens. The overall positive rate of A. ovis, A. bovis, A. phagocytophilum, and T. ovis in M. ovinus was 39.1%, 17.4%, 9.8%, and 89.1%, respectively. All of the samples were negative for Border disease virus (BDV), other Anaplasma species, Babesia spp., Rickettsia spp., and Borrelia spp. Co-infection of different Anaplasma species and T. ovis occurred in 51.2% of all samples with T. ovis. The positive rates of A. ovis, A. bovis, and A. phagocytophilum in different regions and altitudes of the sampling sites were significantly different. Sequence and phylogenetic analysis of target genes confirmed their identity with corresponding pathogens. Our results elucidate the occurrence and molecular characterization of Anaplasma spp. and Theileria spp. in M. ovinus, which could act as potential zoonotic reservoirs. To the best of our knowledge, this is the first report of the detection of A. bovis and A. phagocytophilum DNA in M. ovinus. This study gives the first extensive molecular survey of vector-borne pathogens with veterinary and public health significance in M. ovinus from the Qinghai-Tibet Plateau, China.

scholarly journals Isolation and Characterization of Pb Resistant Bacteria from Cilalay Lake, Indonesia

2015 ◽  
Vol 4 (3) ◽  
Author(s):  
Kesi Kurnia ◽  
Nina Hermayani Sadi ◽  
Syafitri Jumianto
Keyword(s):  
Heterotrophic Bacteria ◽  
Water Environment ◽  
Resistant Bacteria ◽  
Bacterial Isolates ◽  
Gram Negative ◽  
Isolation And Characterization ◽  
Agar Media ◽  
Physical And Chemical ◽  
Standard Disk

<span>Pollution of water environment with heavy metals is becoming one of the most severe environmental and human health hazards. Lead (Pb) is a major pollutant and highly toxic to human, animals, plants, and microbes. </span><span lang="IN">Toxic metals are difficult to remove from the environment, since they cannot be chemically or biologically degraded and are ultimately indestructible. Biological approaches based on metal-resistant microorganisms have received a great deal of attention as alternative remediation processes. </span><span>This study aim to isolat</span><span lang="IN">e</span><span> and characterize Pb resistant of heterotrophic bacteria in Cilalay Lake, </span><span lang="IN">West Java, </span><span>Indonesia. The water samples were collected </span><span lang="IN">along</span><span> three points around Cilalay Lake. </span><span lang="IN">Water physical and chemical </span><span>determination was performed using the Water Quality Checker</span><span lang="IN">. </span><span>The bacterial isolates were screened on T</span><span lang="IN">r</span><span>ipton</span><span lang="IN">e</span><span> Glucose Yeast (TGY) agar plates. </span><span lang="IN">Afterwards s</span><span>elected isolates were grown on Nutrient Agar media 50% </span><span lang="IN">with </span><span>supplemented Pb 100 ppm by the standard disk. Population of resistant bacteria was counted. The result from metal resistant bacteria indicated that all isolates w</span><span lang="IN">ere</span><span> resistant. The most abundant type of resistant </span><span lang="IN">bacteria </span><span>to lead was Gram negative more than Gram positive. Identified have metal resistant bacteria could be useful for the bioremediation of heavy metal contaminated sewage and waste water</span>

Isolation and characterization of nitrate reductase deficient mutants of the ectomycorrhizal fungus Hebeloma cylindrosporum

1998 ◽  
Vol 140 (2) ◽  
pp. 311-318 ◽  
Author(s):  
ROLAND MARMEISSE ◽  
PATRICIA JARGEAT ◽  
FRANCOISE WAGNER ◽  
GILLES GAY ◽  
JEAN-CLAUDE DEBAUD
Keyword(s):  
Nitrate Reductase ◽  
Ectomycorrhizal Fungus ◽  
Hebeloma Cylindrosporum ◽  
Isolation And Characterization

Isolation and characterization of bacteria from the rhizosphere and bulk soil of Stellera chamaejasme L.

2015 ◽  
Vol 61 (3) ◽  
pp. 171-181 ◽  
Author(s):  
Haiyan Cui ◽  
Xiaoyan Yang ◽  
Dengxue Lu ◽  
Hui Jin ◽  
Zhiqiang Yan ◽  
...  
Keyword(s):  
Bulk Soil ◽  
Growth Stages ◽  
Bacterial Isolates ◽  
Phenotypic Properties ◽  
Isolation And Characterization ◽  
Fruiting Stage ◽  
Major Shift ◽  
Stellera Chamaejasme ◽  
Bacterial Groups

This study is the first to describe the composition and characteristics of culturable bacterial isolates from the rhizosphere and bulk soil of the medicinal plant Stellera chamaejasme L. at different growth stages. Using a cultivation-dependent approach, a total of 148 isolates showing different phenotypic properties were obtained from the rhizosphere and bulk soil. Firmicutes and Actinobacteria were the major bacterial groups in both the rhizosphere and bulk soil at all 4 growth stages of S. chamaejasme. The diversity of the bacterial community in the rhizosphere was higher than that in bulk soil in flowering and fruiting stages. The abundance of bacterial communities in the rhizosphere changed with the growth stages and had a major shift at the fruiting stage. Dynamic changes of bacterial abundance and many bacterial groups in the rhizosphere were similar to those in bulk soil. Furthermore, most bacterial isolates exhibited single or multiple biochemical activities associated with S. chamaejasme growth, which revealed that bacteria with multiple physiological functions were abundant and widespread in the rhizosphere and bulk soil. These results are essential (i) for understanding the ecological roles of bacteria in the rhizosphere and bulk soil and (ii) as a foundation for further evaluating their efficacy as effective S. chamaejasme growth-promoting rhizobacteria.

scholarly journals Isolation and Characterization of Thermophilic Bacteria Indigenous to Al-Ahsa Desert

2020 ◽  
Vol 14 (3) ◽  
pp. 2157-2163
Author(s):  
Ahmed Anwar Al-Mulla ◽  
Ashraf Khalifa
Keyword(s):  
Standard Method ◽  
Thermophilic Bacteria ◽  
Soil Samples ◽  
Biotechnological Applications ◽  
Bacterial Isolates ◽  
Phenotypic Characteristics ◽  
Thermostable Enzymes ◽  
Biological Resources ◽  
Isolation And Characterization

Deciphering the biological resources across the Saudi niches is highly recommended for the prosperity. To this end, the aim of the current work was to isolate thermophilic bacteria from unexplored areas of Al-Ahsa region, and investigate their phenotypic characteristics. Three soil samples were collected from different desert sites of Al-Ahsa region. Thermophilic bacteria were isolated directly for soil samples into Thermus medium broth as a standard method. Single colonies of the actively growing bacterial isolates were preserved in 20% glycerol then kept at -80°C. The isolates were screened for production of thermostable enzymes using the commercially available kit API20E strip (bioMerieux, Marcy l’Etoile, France). Incubation were carried out at 50°C. It can be concluded that thermophilic bacteria in Al-Ahsa region harbor novel thermostable enzymes that might have biotechnological applications, in future.

Characterization of the Rickettsia-like and Coxiella-like symbionts in the tick Dermacentor everestianus Hirst, 1926 (Acari: Ixodidae) from the Qinghai-Tibet Plateau

2019 ◽  
Vol 24 (1) ◽  
pp. 106
Author(s):  
Ningxin Li ◽  
Sisi Li ◽  
Duo Wang ◽  
Peng Yan ◽  
Wenying Wang ◽  
...  
Keyword(s):  
Salivary Glands ◽  
Malpighian Tubules ◽  
Tibet Plateau ◽  
The Tibetan Plateau ◽  
Anaplasma Ovis ◽  
Dermacentor Everestianus ◽  
Qinghai Tibet Plateau ◽  
Great Damage

The tick Dermacentor everestianus is widely distributed on the Tibetan Plateau of China, where adult ticks usually parasitize sheep, yaks and horses. D. everestianus is able to transmit many zoonotic pathogens, including Francisella tularensis, Anaplasma ovis and Rickettsia raoultii-like bacteria, and can cause great damage to animals and human health. However, the symbionts in D. everestianus have not yet been investigated, which has hindered our understanding of the relationships between this tick species and associated tick-borne pathogens. In the current study, the Rickettsia-like and Coxiella-like symbionts in D. everestianus were identified and characterized. The results indicated that both Rickettsia-like (RLS-Des) and Coxiella-like (CLS-Des) symbionts showed 100% infection rates and displayed vertical transmission in D. everestianus. The RLS-Des showed a relatively higher abundance than the CLS-Des in D. everestianus. No tissue specificity was found for the RLS-Des or CLS-Des. These symbionts can inhabit the ovaries, salivary glands, midguts, Malpighian tubules and testes of D. everestianus. During the development of D. everestianus, the density of the RLS-Des showed more obvious changes than did that of the CLS-Des. Dramatic changes in the density of the RLS-Des were detected in the midguts, ovaries, salivary glands and Malpighian tubules when female D. everestianus were engorged and detached from the host, which suggested the potential role of these symbionts in the reproduction and development of D. everestianus. The dynamic changes in the density of the CLS-Des during feeding and reproduction of D. everestianus suggest the involvement of the CLS-Des in the reproduction of D. everestianus. 

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