scholarly journals Screening and Joint Analysis of Key lncRNAs for Milk Fat Metabolism in Dairy Cows

2021 ◽  
Author(s):  
Tong Mu ◽  
Honghong Hu ◽  
Xiaofang Feng ◽  
Yanfen Ma ◽  
Ying Wang ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Regulatory Network ◽  
Milk Fat ◽  
Target Genes ◽  
Fat Metabolism ◽  
Enrichment Analysis ◽  
Joint Analysis ◽  
Differential Gene ◽  
Fat Synthesis ◽  
Milk Fat Synthesis

Abstract Background: Long noncoding RNAs (lncRNAs) play an important regulatory role in various biological processes as a key regulatory factor. However, there are largely unknown for the function and expression profile of lncRNAs in milk fat synthesis of dairy cows. Results: In this study, RNA sequencing (RNA-seq) was used to research the whole genome expression of lncRNAs and mRNA transcripts in bovine mammary epithelial cells (BMECs) of dairy cows with high and low milk fat percentage (MFP), and joint analysis was carried out. We identified a total of 47 differentially expressed genes (DEGs) and 38 differentially expressed lncRNAs (DELs, Padj < 0.05), 11 candidate DEGs that may regulate milk fat metabolism were screened by enrichment analysis. Downregulated differential gene ENPP2 and upregulated differential gene BCAT1 are more likely to participate in the milk fat metabolism, and its function needs further experiments verification. The enrichment analysis of target genes predicted by DELs identified 7 cis (co-localization) and 10 trans (co-expression) candidate target genes related to milk lipid metabolism, corresponding to a total of 18 DELs. Among them, the targeting relationship between long intervening/intergenic noncoding RNA (lincRNA) TCONS_00082721 and FABP4 gene that predicts milk fat metabolism by co-localization and co-expression is worthy of attention. Based on the expression information of DELs, differential microRNAs (miRNAs), and lipid metabolism-related target genes, 156 competing endogenous RNAs (ceRNAs) interaction regulation networks related to milk fat metabolism were constructed. The regulatory network centered on miR-145 will be the focus of subsequent experimental research. The ceRNAs regulatory network related to TCONS_00082721 and TCONS_00172817 are more likely to be involved in milk fat synthesis. Conclusions: These results will provide new ways to understand the complex biology of dairy cow milk fat synthesis and provide valuable information for the breed improvement of Chinese Holstein cattle.

Characterisation of gene expression related to milk fat synthesis in the mammary tissue of lactating yaks

2017 ◽  
Vol 84 (3) ◽  
pp. 283-288 ◽  
Author(s):  
Jung Nam Lee ◽  
Yong Wang ◽  
Ya Ou Xu ◽  
Yu Can Li ◽  
Fang Tian ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dairy Cows ◽  
Lipid Droplet ◽  
Milk Fat ◽  
De Novo ◽  
Droplet Formation ◽  
Synthesis Process ◽  
Lipid Droplet Formation ◽  
Fat Synthesis ◽  
Milk Fat Synthesis

This research communication describes the profile of gene expression related to the synthesis of yak milk as determined via quantitative reverse transcription polymerase chain reaction (RT-qPCR). Significant up-regulation during lactation were observed in genes related to fatty acid (FA) uptake from blood (LPL, CD36), intracellular FA transport (FABP3), intracellular FA activation of long- and short-chain FAs (ACSS1, ACSS2, ACSL1), de novo synthesis (ACACA), desaturation (SCD), triacyglycerol (TAG) synthesis (AGPAT6, GPAM, LPIN1), lipid droplet formation (PLIN2, BTN1A1, XDH), ketone body utilisation (BDH1, OXCT1), and transcription regulation (THRSP, PPARGC1A). In particular, intracellular de novo FA synthesis (ACSS2, ACACA, and FABP3) and TAG synthesis (GPAM, AGPAT6, and LPIN1), whose regulation might be orchestrated as part of the gene network under the control of SERBF1 in the milk fat synthesis process, were more activated compared to levels in dairy cows. However, the genes involved in lipid droplet formation (PLIN2, XDH, and BTN1A1) were expressed at lower levels compared to those in dairy cows, where these genes are mainly controlled by the PPARG regulator.

Kisspeptin-10 Promoting the Synthesis of Milk Fat by Inhibiting the AMPK/SIRT6 Signaling Pathway via the GPR54 in Bovine Mammary Epithelial Cells

2020 ◽  
Author(s):  
Yu Cao ◽  
Juxiong Liu ◽  
Qing Zhang ◽  
Lijun Ma ◽  
Jiaxin Wang ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Signaling Pathway ◽  
Milk Fat ◽  
Mammary Epithelial Cells ◽  
Peptide Hormone ◽  
Underlying Mechanism ◽  
Mammary Glands ◽  
Bovine Mammary Epithelial Cells ◽  
Fat Synthesis ◽  
Milk Fat Synthesis

Abstract Background Kp-10 is a peptide hormone mainly involved in the initiation tissue development in puberty. Recent studies have shown that Kp-10 is involved in fat synthesis. However, the role of Kp-10 in milk fat synthesis in lactating dairy cows has not been reported. Therefore, this study investigated the correlation between GPR54 and milk fat synthesis in dairy cows and to study the underlying mechanism in BMECs. Results The results showed that the expression of GPR54, SREBP1 and FASN in mammary glands of high-milk fat dairy cows were significantly higher than those in mammary glands of low-milk fat dairy cows. Meanwhile, 10nM Kp-10 can significantly inhibit AMPK/SIRT6 signaling pathway and promote milk fat synthesis in BMECs through its receptor GPR54. Overexpression of SIRT6 significantly reduced the acetylation level of SREBP1 and milk fat synthesis in BMECs.Conclusions These results suggested that Kp-10 inhibits the AMPK / SIRT6 signaling pathway by mediating GPR54, thereby increasing SREBP1 acetylation levels and increasing milk fat synthesis in BMECs.

Effect of abomasal infusions of geometric isomers of 10,12 conjugated linoleic acid on milk fat synthesis in dairy cows

Lipids ◽  
2005 ◽  
Vol 40 (8) ◽  
pp. 823-832 ◽  
Author(s):  
Asgeir Sæbø ◽  
Per-Christian Sæbø ◽  
J. Mikko Griinari ◽  
Kevin J. Shingfield
Keyword(s):  
Linoleic Acid ◽  
Dairy Cows ◽  
Conjugated Linoleic Acid ◽  
Milk Fat ◽  
Geometric Isomers ◽  
Fat Synthesis ◽  
Milk Fat Synthesis

scholarly journals 2,4-Thiazolidinedione Treatment Improves the Innate Immune Response in Dairy Goats with Induced Subclinical Mastitis

PPAR Research ◽  
2017 ◽  
Vol 2017 ◽  
pp. 1-22 ◽  
Author(s):  
Fernanda Rosa ◽  
Johan S. Osorio ◽  
Erminio Trevisi ◽  
Francisco Yanqui-Rivera ◽  
Charles T. Estill ◽  
...  
Keyword(s):  
Milk Fat ◽  
Target Genes ◽  
Subclinical Mastitis ◽  
Economic Losses ◽  
Minor Effect ◽  
Dairy Goats ◽  
Nonesterified Fatty Acids ◽  
Lactating Goats ◽  
Fat Synthesis ◽  
Milk Fat Synthesis

Mastitis is a major disease in dairy cows resulting in significant economic losses. In vitro works suggest that ruminants peroxisome proliferator-activated receptor gamma (PPARγ) can aid in improving the response to mastitis and can control milk fat synthesis. The objectives of the present experiment were to test if treatment with the putative PPARγ agonist 2,4-thiazolidinedione (TZD) improves (1) the response to subclinical mastitis and (2) milk fat production. Lactating goats received daily injections of 8 mg/kg BW of TZD or saline for 3 weeks. After one week of TZD injection, half of the goats in each group received intramammary infusion of Strep. uberis or saline in both halves for a total of 4 groups (n=6/group). TZD treatment did not affect milk fat but had positive effect on milk somatic cells count, blood nonesterified fatty acids, inflammatory markers, and liver function. TZD significantly increased myeloperoxidase but did not affect leukocytes phagocytosis or insulin. TZD increased adipocytes size and had minor effect on expression of PPARγ target genes in mammary epithelial cells but not in adipose tissue. Overall, TZD ameliorated the response to intramammary infection but the effect on milk fat synthesis and expression of related transcripts was less than expected.

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