Connection between Gab1 Polymorphisms and cholangiocarcinoma patients prognosis

Abstract Background We conducted this study to measure the levels of GRB2 associated binding protein 1 (Gab1) mRNA in cholangiocarcinoma (CCA) tissues and the paired normal tissues. And then assess the prognostic significance of Gab1 in CCA patients. Methods Quantitative real-time (qRT-PCR) was adopted to confirm the expression levels of Gab1 mRNA in both CCA tissues and normal controls. The Chi-square test was applied to estimate the influence of clinical parameters on Gab1 expression. Kaplan-Meier survival analysis with log-rank test were used to determine overall survival rates of patients with different Gab1 expression. Cox regression analysis was carried out to evaluate prognostic value of factors in CCA patients. Results According to the qRT-PCR result, increased expression of Gab1 mRNA was found in CCA tissues compared with the paired noncancerous controls (P<0.0001). The Chi-square test demonstrated that the elevated expression of Gab1 was affected by poor differentiation (P=0.022), positive lymph node metastasis (P=0.008) and high TNM stage (P=0.004). However, age, gender, family history and resection margin had no significant influence on Gab1 levels (all, P>0.05). The survival curves suggested that up-regulation of Gab1 was related with poor prognosis of CCA patients. Furthermore, the Cox analysis demonstrated Gab1 was a prognostic biomarker for CCA patients (P=0.000, 95%CI=2.576, HR=1.552-4.275). Conclusion Taken together, Gab1 expression was increased in CCA and may be a useful biomarker to predict the outcomes of CCA patients.

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The expression of HOXA9 and its prognostic value in cervical cancer

10.21203/rs.3.rs-54158/v1 ◽

2020 ◽

Author(s):

Keqian Zhang ◽

Tianqi Mao ◽

Zhicheng He ◽

Xiaojiao Wu ◽

Yu Peng ◽

...

Keyword(s):

Cervical Cancer ◽

Prognostic Value ◽

Cox Regression ◽

Figo Stage ◽

Rank Test ◽

Chi Square ◽

Cox Regression Analysis ◽

Normal Tissues ◽

Kaplan Meier ◽

Chi Square Test

Abstract Background The HOXA9 gene, belonging to homeobox (HOX) gene family, has been recently reported dys-expressed in several kinds of human cancers. This study aimed to investigate the expression of HOXA9 and its prognostic value in cervical cancer. Methods The HOXA9 mRNA expression was detected with a quantitative real-time polymerase chain reaction (qRT-PCR) assay, and the association of HOXA9 expression with clinical characteristic was analyzed via chi-square test. Kaplan-Meier and cox regression analyses were conducted to estimate the prognostic value of HOXA9 in cervical cancer. Results HOXA9 expression was significantly down-expressed in cervical cancer tissues compared with that in adjacent normal tissues (P < 0.01). And the expression of HOXA9 was significantly associated with TNM stage, pathological grade, FIGO stage and differentiation (All P < 0.05). In addition, Kaplan–Meier analysis indicated that the overall survival of patients with low HOXA9 expression was shorter than those with high HOXA9 expression (log rank test, P = 0.000). Cox regression analysis revealed that HOXA9 had a high prognostic value in cervical cancer. Conclusion HOXA9 is down-regulated and involved in the development of cervical cancer. Moreover, it may be an useful independent prognostic bio-marker for patients with cervical cancer.

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Rab25 Acts as a Promising Novel Bio-Marker in the Prognosis of Patients with Hepatocellular Carcinoma

10.21203/rs.3.rs-43510/v1 ◽

2020 ◽

Author(s):

Zhong Dai ◽

Ke-Qing Yao ◽

Xing-Sheng Hu ◽

Yi-Qun Li ◽

Yu-Tao Liu ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Prognostic Value ◽

Cox Regression ◽

Rank Test ◽

Chi Square ◽

Cox Regression Analysis ◽

Normal Tissues ◽

Kaplan Meier ◽

Chi Square Test ◽

The Relationship

Abstract Background: Rab25 was indicated to be involved in several human tumors. However, the clinical significance of Rab25 in hepatocellular carcinoma (HCC) was still unclear. The purpose of this study was to investigate the expression and prognostic value of Rab25 in HCC.Methods: The relative mRNA expression levels of Rab25 in HCC tissues and adjacent normal tissues were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Chi-square test was used to analyze the relationship between Rab25 expression and clinical characteristics of patients. The prognostic value of Rab25 in HCC was estimated through Kaplan-Meier method and cox regression analysis.Results: Rab25 gene expression level was significantly higher in HCC tissues than that in normal tissues (P<0.001). Importantly, the increased Rab25 expression was closely associated with TNM stage (P=0.024), metastasis (P=0.022) and invasion classification (P=0.039). Moreover, patients with high Rab25 expression tended to have obviously shorter overall survival than those with low expression of Rab25 (log rank test, P<0.001) via Kaplan-Meier analysis. Univariate and multivariate cox regression analyses revealed that Rab25 was an independent prognostic factor of HCC.Conclusions: Rab25 is up-regulated in HCC and contributes to the progression of this tumor. What’s more, Rab25 may be a potential bio-marker for the prognosis of HCC.

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CIP4 Expression is Associated With The Prognosis in Colorectal Cancer

10.21203/rs.3.rs-95046/v1 ◽

2020 ◽

Author(s):

Keqian Zhang ◽

Tianqi Mao ◽

Zhicheng He ◽

Xiaojiao Wu ◽

Yu Peng ◽

...

Keyword(s):

Colorectal Cancer ◽

Cox Regression ◽

Prognostic Significance ◽

Lymphatic Invasion ◽

Chi Square ◽

Interacting Protein ◽

Cox Regression Analysis ◽

Kaplan Meier ◽

Chi Square Test ◽

Student’S T

Abstract Background: This study was conducted to detect the expression of Cdc42 interacting protein 4 (CIP4) in patients with colorectal cancer (CRC), and explore the role of CIP4 in prognosis of CRC patients.Methods: The expression of CIP4 mRNA was determined by quantitative real-time PCR (qRT-CPR) and compared by student’s t-test between groups. Relationships of clinical characteristics and CIP4 expression were analyzed by Chi-square test. Kaplan-Meier curves were used to estimate the overall survival of CRC patients. And Cox regression analysis was conducted to identify the prognostic biomarkers for CRC patients.Results: The qRT-PCR results showed that CRC tissues were detected with significantly high CIP4 mRNA expression compared with adjacent normal controls (P<0.0001). The overexpression of CIP4 in CRC tissues was influenced by distant metastasis (P=0.021), lymphatic invasion (P=0.012) and TNM stage (P=0.006). But, other clinical factors including age, gender, differentiation and tumor site were proved to have no obvious effects on CIP4 expression (all, P>0.05). The survival curves showed that patients with high CIP4 expression generally lived shorter than those with low CIP4 expression (P<0.001). In addition, the multivariate analysis revealed that differentiation (P=0.044, HR=1.631, 95%CI=1.013-2.626) and CIP4 expression (P=0.000, HR=5.283, 95%CI=3.138-8.893) were of great prognostic significance for CRC patients.Conclusion: Taken together, up-regulation of CIP4 in CRC tissues represented poor prognosis for patients.

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Comparison of endoscopic ultrasound-guided hepaticogastrostomy and the antegrade technique: study protocol for a prospective, multicentre, randomised controlled trial

10.21203/rs.2.18009/v1 ◽

2019 ◽

Author(s):

Ye Liao ◽

Tingyue Xiao ◽

Shiyun Sheng ◽

Yufan Wu ◽

Jingjing Zhang ◽

...

Keyword(s):

Endoscopic Ultrasound ◽

Cox Regression ◽

Controlled Trial ◽

Rank Test ◽

Ultrasound Guided ◽

Clinical Trial Registry ◽

Chi Square ◽

Cox Regression Analysis ◽

Chi Square Test ◽

Randomised Controlled

Abstract Background: Endoscopic ultrasound-guided biliary drainage (EUS-BD) is used after failed endoscopic retrograde cholangiopancreatography. Based on existing studies, intrahepatic (IH) approaches are suggested to be preferred in patients with a dilated IH bile duct. Both ultrasound-guided hepaticogastrostomy (EUS-HGS) and ultrasound-guided antegrade treatment (EUS-AG) are available IH approaches for patients with an unreachable papilla. However, a direct comparison between these two approaches is lacking. Therefore, we aim to evaluate and compare the safety and efficiency of EUS-HGS and EUS-AG in patients with an unreachable papilla. Methods: This is a prospective, randomised, controlled, multicentre study. One-hundred forty-eight patients from three hospitals who met the inclusion criteria will be randomly assigned (1:1) to undergo either EUS-HGS or EUS-AG for relief of biliary obstruction. The final study follow-up is scheduled at 1 year postoperatively. The primary endpoint is to compare the safety and efficiency of EUS-HGS and EUS-AG in patients with an unreachable papilla. The secondary endpoint is the overall survival rate of patients undergoing EUS-HGS and EUS-AG. The chi-square test, Kaplan-Meier methods, the log-rank test, and Cox regression analysis will be used to analyse the data. Discussion: To our knowledge, this is the first study to compare these two EUS-BD approaches directly using a multicentre, randomized, controlled trial design. The clinical economic indexes will also be compared, as they may also affect the patient’s choice. The result may contribute to establishing a strategic guideline for choosing IH EUS-BD approaches. Trial registration: Chinese Clinical Trial Registry (ChiCTR), ChiCTR1900020737; registered on 15 January 2019. http://www.chictr.org.cn/showproj.aspx?proj=33672

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Development of predictive prognostic nomogram for NECs of rectum on population-based exploration

Endocrine Connections ◽

10.1530/ec-18-0353 ◽

2018 ◽

Vol 7 (11) ◽

pp. 1178-1185 ◽

Cited By ~ 1

Author(s):

Yang Lv ◽

Ning Pu ◽

Wei-lin Mao ◽

Wen-qi Chen ◽

Huan-yu Wang ◽

...

Keyword(s):

Cox Regression ◽

Population Based ◽

Prognostic Indicators ◽

Rank Test ◽

Chi Square ◽

Related Factors ◽

Ki 67 ◽

Cox Regression Analysis ◽

Kaplan Meier ◽

Chi Square Test

Aim We aim to investigate the clinical characteristics of the rectal NECs and the prognosis-related factors and construct a nomogram for prognosis prediction. Methods The data of 41 patients and 1028 patients with rectal NEC were retrieved respectively from our institution and SEER database. OS or PFS was defined as the major study outcome. Variables were compared by chi-square test and t-test when appropriate. Kaplan–Meier analysis with log-rank test was used for survival analysis and the Cox regression analysis was applied. The nomogram integrating risk factors for predicting OS was constructed by R to achieve superior discriminatory ability. Predictive utility of the nomogram was determined by concordance index (C-index) and calibration curve. Results In the univariate and multivariate analyses, tumor differentiation, N stage, M stage and resection of primary site were identified as independent prognostic indicators. The linear regression relationship was found between the value of Ki-67 index and the duration of OS (P < 0.05). Furthermore, the independent prognostic factors were added to formulate prognostic nomogram. The constructed nomogram showed good performance according to the C-index. Conclusions Contrary to WHO classification guideline, we found that the rectal NEC diseases are heterogeneous and should be divided as different categories according to the pathological differentiation. Besides, the nomogram formulated in this study showed excellent discriminative capability to predict OS for those patients. More advanced predictive model for this disease is required to assist risk stratification via the formulated nomogram.

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PCAT-1 Expression is Associated With The Prognosis in Prostate Cancer.

10.21203/rs.3.rs-112933/v1 ◽

2020 ◽

Author(s):

Sheng Li ◽

Xiaolan Ruan ◽

Tongzu Liu

Keyword(s):

Prostate Cancer ◽

Cox Regression ◽

Prognostic Biomarker ◽

Univariate Analysis ◽

Prognostic Significance ◽

Survival Curves ◽

Chi Square ◽

Cox Regression Analysis ◽

Chi Square Test ◽

Cox Analysis

Abstract Purpose: In the study, we aimed to estimate the prognostic significance of PCAT-1 in patients with prostate cancer (PCa).Methods: The expression of PCAT-1 in paired PCa tissues and normal controls was examined via quantitative real-time polymerase chain reaction (qRT-PCR). The influence of PCAT-1 level on clinical features was assessed using Chi-square test. The survival curves were plotted to estimate the prognosis of patients. And the Cox analysis was carried out to find promising predictive factors for patients.Results: The expression level of PCAT-1 in PCa tissues was significantly elevated compared with the adjacent normal control (P<0.0001). The increased expression of PCAT-1 was affected by high Gleason score (P=0.017), positive serum PSA (P=0.011) and advanced TNM stage (P=0.003). The Kaplan-Meier survival curves showed that the overall survival rate of patients with high PCAT-1 expression was significantly lower than those with low PCAT-1 expression (P<0.001). Both univariate analysis (P=0.000, HR=10.623, 95%CI=5.798-19.464) and multivariate Cox regression analysis (P=0.000, HR=10.996, 95%CI=5.896-20.507) revealed that PCAT-1 could act as a prognostic biomarker for PCa patients.Conclusion: Taken together, overexpression of PCAT-1 is involved in PCa progression and could be a potential prognostic biomarker for PCa patients.

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Prognostic significance of TRIM28 expression in patients with breast carcinoma

Open Medicine ◽

10.1515/med-2021-0263 ◽

2021 ◽

Vol 16 (1) ◽

pp. 472-480

Author(s):

Wen Zhang ◽

Zhengquan Cai ◽

Mingzhu Kong ◽

Anqi Wu ◽

Zeyang Hu ◽

...

Keyword(s):

Breast Carcinoma ◽

Cox Regression ◽

Molecular Subtype ◽

Prognostic Significance ◽

Progression Free Survival ◽

Chi Square ◽

Normal Tissues ◽

Reverse Transcription Pcr ◽

Chi Square Test ◽

And Function

Abstract Background Tripartite motif 28 (TRIM28) plays a role in multiple biological functions. The expression and function of TRIM28 in breast carcinoma (BC) remain unclear. The aim of this study was to explore potential association of TRIM28 with tumor features and survival. Materials and methods Specimens were collected from BC and adjacent normal tissues. Quantitative reverse transcription PCR (RT-qPCR) and immunohistochemistry (IHC) were performed to detect TRIM28 expression. The correlation of TRIM28 with clinicopathological features was evaluated by Chi-square test. The relationship between TRIM28 expression and survival was further analyzed by the Kaplan-Meier and Cox regression method. A receiver operating characteristic (ROC) curve was used to assess the value of TRIM28 in predicting BC. Results In this retrospective research, it was demonstrated that TRIM28 was overexpressed in BC tissues. TRIM28 overexpression was correlated with lymph node metastasis, advanced TNM stage, and poor molecular subtype. The survival analysis showed that overall survival (OS) and progression-free survival (PFS) were significantly shorter in TRIM28-positive group. Moreover, TRIM28 was an independent prognostic factor for BC. And ROC analysis verified the diagnostic role of TRIM28 in BC. Conclusions TRIM28 is overexpressed in BC and might be a promising prognostic and diagnostic biomarker of BC.

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Analysis of Scoring Sequences in Matches of the Portuguese Premier League

Journal of Human Kinetics ◽

10.1515/hukin-2017-0199 ◽

2018 ◽

Vol 64 (1) ◽

pp. 255-263

Author(s):

José M. Pratas ◽

Anna Volossovitch ◽

Ana I. Carita

Keyword(s):

Regression Analysis ◽

Cox Regression ◽

Cox Model ◽

Positive Association ◽

Descriptive Statistics ◽

Chi Square ◽

Cox Regression Analysis ◽

Premier League ◽

Chi Square Test

AbstractThe aim of this study was to examine the sequences of the first two goals scored in soccer matches in accordance with a range of different match contexts. Data from 1506 matches played in the Portuguese Premier League during six consecutive competitive seasons (2009-10 to 2014-2015) were analysed using descriptive statistics and the chi-square test in order to verify the association between variables and a Cox regression analysis was used to predict the time the second goal was scored in function of the time of the first goal scored in the match and the scoreline. The results revealed a higher frequency of the second goals being scored in the second half of a match (58%) and in the last 5 min periods of each half. A positive association was found for home teams and score-doubling goals (58%), as well as for away teams and score-equalizing goals (56%). For home and away teams the score-doubling goal of a match was strongly and positively associated with a win outcome for home (93%) and away teams (92%), while the score-equalizing goals were associated with a draw (home and away teams: 44%) and loss outcome (home: 33% and away teams: 32%). Finally, the Cox model showed that if the first goal was scored in the second half of the match, the probability of the second goal being scored was three times higher compared to the first half.

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Expression of MUM1/IRF4 correlates with clinical outcome in patients with B-cell chronic lymphocytic leukemia

Blood ◽

10.1182/blood-2002-01-0104 ◽

2002 ◽

Vol 100 (13) ◽

pp. 4671-4675 ◽

Cited By ~ 39

Author(s):

Chung-Che Chang ◽

Jennifer Lorek ◽

Daniel E. Sabath ◽

Ying Li ◽

Christopher R. Chitambar ◽

...

Keyword(s):

Chronic Lymphocytic Leukemia ◽

B Cell ◽

Cox Regression ◽

Prognostic Significance ◽

Lymphocytic Leukemia ◽

Rank Test ◽

Cox Regression Analysis ◽

Kaplan Meier ◽

Interferon Regulatory Factor 4 ◽

Cell Chronic Lymphocytic Leukemia

In this study, we evaluated the prognostic significance of multiple myeloma-1/interferon regulatory factor-4 (MUM1/IRF4) expression in B-cell chronic lymphocytic leukemia (B-CLL). Our results demonstrated that the absence of MUM1/IRF4 expression showed the highest relative risk among the factors analyzed in determining the probability for death in patients with B-CLL using univariate and multivariate Cox regression analysis. Patients without MUM1/IRF4 expression had significantly worse overall survival than did those with MUM1/IRF4 expression (52% cumulative survival, 63 months vs not reached, Kaplan-Meier survival analysis; P < .03, log-rank test). Patients with MUM1/IRF4 expression were more likely to have disease at low Rai stage and interstitial/nodular marrow involvement. Furthermore, only 1 of 11 patients with MUM1/IRF4 expression and interstitial/nodular marrow involvement died during a 100-month follow-up. Our results suggest that B-CLL with expression of MUM1/IRF4, indicative of postgerminal center origin, has a more favorable clinical course and that MUM1/IRF4 is an important prognostic marker in B-CLL.

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A comparison of the prognostic significance of changes in NT-proBNP levels in HFrEF and HFpEF.

10.22541/au.161978616.62769552/v1 ◽

2021 ◽

Author(s):

Nishant Sahni ◽

Umesh Sharma ◽

Rashi Arora

Keyword(s):

Regression Models ◽

Cox Regression ◽

Survival Rates ◽

Prognostic Significance ◽

Rank Test ◽

Log Rank Test ◽

Kaplan Meier ◽

Regional Health Care ◽

Comorbidity Scores ◽

Blood Pressures

Background: Rising NT-proBNP are associated with reduced survival patients with HFrEF. However, it remains to be conclusively and formally demonstrated that the temporal trend in NT-proBNP level carries prognostic significance in HFpEF. Objective: To determine whether there is an association between rising NT-proBNP levels and 6-month survival in patients with HFpEF and HFrEF. Methods: We examined a cohort of 5203 patients to 5 hospitals in a regional health care system — who had at least one admission to the hospital with diagnoses of heart failure over a 3-year period. Kaplan-Meier survival curves were constructed for patients with downtrending (>25% net decrease), stable or uptrending (>25% net increase) NT-proBNP levels in HF, HFpEF and HFrEF patients. The log-rank test was used to test for differences in 6-month survival amongst the groups. Multivariate extended Cox regression models were constructed for 6-month survival with NT-proBNP as a time-varying covariate. Age, albumin, sex, race, serum creatinine, systolic and diastolic blood pressures and Charlson comorbidity scores at baseline were used as covariates in the model. Separate analyses were done for HFpEF and HFrEF patients. Results: HFpEF and HFrEF patients with up-trending levels had significantly lower 6-month survival rates than patients with downtrending or stable NT-proBNP levels. A doubling of the NT-proBNP level in patients was significantly associated with reduced 6-month survival in patients with in both subgroups of HF, HFpEF and HFrEF (HFpEF-HR: 1.53(1.49-2.57), HFrEF HR: 1.45(1.43-1.48) after adjusting for covariates.

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