Biomimetic Recombinant of Red Blood Cell Membranes for Improved Photothermal Therapy
Abstract Background: RBC membrane derived nanoparticles (NPs) represent an emerging platform with prolonged circulation capacity for the delivery of active substances. For endowing derived RBCs NPs various functions such as targeting ability, chemical modification of targeting groups or inserting targeted ligand-linker-lipid conjugates into the membranes is usually used as classical strategy. However, these methods may lead to structural failure of membrane proteins, consequently affecting its original immune escape function.Results: In this study, we reported a green technology of “disassembly-reassembly” to prepare biomimetic reconstituted RBCs membrane (rRBCs) by separating the endogenous proteins and lipids from nature RBC membrane. IR780 iodide was used as a pattern drug to verify the property and feasibility of rRBCs by constructing IR780@rRBC NPs with IR780@RBC NPs and free IR780 as controls. The results demonstrated the superiority of IR780@rRBC NPs in toxicity, stability, pharmacokinetics and pharmacodynamics compared with IR780@rRBC and free IR780.Conclusions: The reported “disassembly-reassembly” strategy shows great potential to produce controllable and versatile rRBC membrane-inspired delivery platform, which may be used to overcome the deficiency of target-selectivity ligands in cell membrane coated nanoparticles.