scholarly journals Agronomic Performance of Temporary Immersion Bioreactor – Derived Potato Microtubers in a Peruvian Low Input Cropping Agriculture System

2021 ◽  
Author(s):  
María de Lourdes Tapia y Figueroa ◽  
José Faustino Beraún Tapia ◽  
Elliosha Hajari ◽  
Maritza Escalona ◽  
Hervé Etienne ◽  
...  
Keyword(s):  
Commercial Production ◽  
Agronomic Performance ◽  
Liquid Media ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion ◽  
Low Input ◽  
Seed Tubers ◽  
Planting Material ◽  
Propagation Material

Abstract Potato cultivation is limited by a lack of access to quality propagation material. The application of micropropagation techniques combined with the diagnosis and sanitation of the main pathogens of the crop, has contributed to increased production efficiencies. In this regard, the use of temporary immersion bioreactors (TIBs) has improved the quality of microtubers micropropagated along with savings in costs of production. With the final goal of applying these technologies for commercial production, the current study investigated the agronomic performance of Peruvian Canchan potato microtubers derived from TIBs (basic agamic seed 1 and 2) under low-input agro-technology in the coastal zone of Peru. The results indicated that following 75 d of growth, plants derived from microtubers produced in TIBs displayed slower vegetative growth than those from conventional tubers. However, at harvest, these differences were no longer apparent. Although plants raised from conventional tubers produced the highest fresh mass of tubers, significantly more propagules were produced by plants regenerated from basic agamic seed 1 and 2 derived from micropropagation in liquid media. These results demonstrate that much more planting material (seed tubers) can be obtained from microtubers in the field (basic agamic seed 1) than from the conventional commercial seed tubers.

scholarly journals Studies on the Occurrence of Viruses in Planting Material of Grapevines in Southwestern Germany

Viruses ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 248
Author(s):  
Noemi Messmer ◽  
Patricia Bohnert ◽  
Stefan Schumacher ◽  
René Fuchs
Keyword(s):  
Preventive Measures ◽  
Control Measures ◽  
Grapevine Fanleaf Virus ◽  
Arabis Mosaic Virus ◽  
Planting Material ◽  
General Decline ◽  
Grapevine Pinot Gris Virus ◽  
Propagation Material

Viral diseases in viticulture lead to annual losses in the quantity and quality of grape production. Since no direct control measures are available in practice, preventive measures are taken to keep the vines healthy. These include, for example, the testing of propagation material for viruses such as Arabis mosaic virus (ArMV), Grapevine fanleaf virus (GFLV) or Grapevine leafroll-associated virus 1 (GLRaV-1) and 3 (GLRaV-3). As long-term investigations have shown, GLRaV-1 (2.1%) occurs most frequently in southwestern German wine-growing regions, whereas GLRaV-3 (<0.1%) is almost never found. However, tests conducted over 12 years indicate that there is no general decline in virus-infected planting material. Thus, it can be assumed that a spread of the viruses via corresponding vectors still takes place unhindered. Beyond the examinations regulated within the German Wine Growing Ordinance, one-time tests were carried out on Grapevine Pinot gris virus (GPGV). This analysis showed that GPGV was found in 17.2% of the samples.

scholarly journals A novel temporary immersion bioreactor system for large scale multiplication of banana (Rasthali AAB—Silk)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Subbaraya Uma ◽  
Raju Karthic ◽  
Sathiamoorthy Kalpana ◽  
Suthanthiram Backiyarani ◽  
Marimuthu Somasundaram Saraswathi
Keyword(s):  
Large Scale ◽  
Shoot Proliferation ◽  
Cost Effective ◽  
Culture Method ◽  
Inter Simple Sequence Repeat ◽  
Scale Production ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion ◽  
Planting Material ◽  
Bioreactor System

AbstractMusa sp. cultivar Rasthali (Silk AAB) is a choice variety of the Asian sub-continent. Its production and sustenance are threatened by Fusarium wilt, which affects the livelihoods of small and marginal farmers. The use of quality planting material is one of the strategies to manage the disease. Availability of quality planting material for varieties other than Grand Naine is limited. Large-scale micropropagation using existing technologies is laborious and expensive. Temporary immersion bioreactor system is emerging as a potential advancement in the micropropagation industry. In this study, a cost-effective temporary immersion bioreactor (TIB) system has been developed and an efficient micropropagation method has been standardized. Explants cultured in TIB with 250 ml of culture medium in a 2-min immersion frequency of 6 h were found to be efficient for shoot proliferation and rooting. Its efficacy has been compared with the semisolid culture method. At the end of the 6th subculture, 1496 ± 110 shoots per explant were obtained in TIB. Chlorophyll, carotenoid, stomatal index, and the number of closed stomata were examined to determine the physiological functions of the plants grown in TIB and compared with semisolid grown plantlets. Plantlets grown in TIB were genetically stable and were confirmed using inter-simple sequence repeat (ISSR) markers. The multiplication of shoots in TIB was 2.7-fold higher than the semisolid culture method, which is suitable for large-scale production of planting material for commercial applications.

scholarly journals Micropropagation of Raspberries (Rubus idaeus L.) in Liquid Media by Temporary Immersion Bioreactor in Comparison with Gelled Media

2021 ◽  
Vol 78 (2) ◽  
pp. 56
Author(s):  
Doina CLAPA ◽  
Monica HÂRȚA ◽  
Cornel Viorel POP
Keyword(s):  
Large Scale ◽  
Rubus Idaeus ◽  
Red Raspberry ◽  
Liquid Media ◽  
Temporary Immersion Bioreactor ◽  
In Vitro Proliferation ◽  
Axillary Shoots ◽  
Temporary Immersion ◽  
Experimental Treatments

Temporary Immersion Bioreactor (TIB) is a suitable technique for large scale micropropagation of plant species. The aim of this work was to test the capacity of in vitro proliferation of the primocane-fruiting red raspberry cv Maravilla and floricane-fruiting red raspberry cv Willamette on gelled media compared to liquid media. The two varieties were cultured in vitro on two media, Murashige and Skoog 1962 (MS) and Driver and Kuniyuki walnut medium, 1984 (DKW), both supplemented with 0.5 mg/l 6-benzyladenine (BA). In the control cultures on gelled media the media were gelled with 5g/l Plant Agar, whereas for the cultures in liquid media Plantform bioreactors were used. After six weeks of in vitro culture we recorded the proliferation rates and lengths of the axillary shoots obtained in all the experimental treatments. The highest proliferation rate was 16 ± 2.03, obtained in cv. Willamette on gelled MS medium with 0.5 mg/l BA. The longest shoots (3.17 ± 0.32 cm) were obtained at cv. Maravilla on the DKW medium with 0.5 mg / l BA in the bioreactor. Our research highlighted that Rubus idaeus L. Maravilla and Willamette can be TIB propagated, although further research is needed to improve the efficiency of this method.

Daminozide Enhances Vigor and Steviol Glycoside Yield of Stevia (Stevia rebaudiana Bert.) Propagated in the Temporary Immersion Bioreactor

2022 ◽  
Author(s):  
Rizka Tamania Saptari ◽  
Rizkita Rachmi Esyanti ◽  
Riza Arief Putranto
Keyword(s):  
Stevia Rebaudiana ◽  
Feedback Regulation ◽  
Optimum Concentration ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion ◽  
Steviol Glycoside ◽  
Kaurenoic Acid ◽  
Biosynthesis Gene ◽  
Inhibitor Treatment ◽  
Ga Biosynthesis

Abstract Stevia (Stevia rebaudiana Bertoni) contains sweet compound widely used as natural sweetener, steviol glycoside (SG). SG is a diterpenoid secondary metabolite synthesized from ent-kaurenoic acid, the same precursor of Gibberellin (GA). Therefore, in this study, a GA inhibitor, Daminozide (0, 10, 20 ppm) was used to block ent-kaurenoic acid conversion towards GA synthesis in attempt to increase SG content of stevia propagated in Temporary Immersion Bioreactor (TIB). Daminozide in 10 mg/L was observed to be the optimum concentration which increased biomass weight and SG content (stevioside and rebaudioside A) up to 40%. The treatment also increased transcripts accumulation of genes enrolled in SG biosynthesis, such as SrKA13H, SrUGT85C2, and SrUGT76G1, indicating SG pathway become more active due to the inhibition of GA pathway. Furthermore, the inhibition of GA was also indicated by the upregulated expression of GA biosynthesis gene (GA3ox) as the result of feedback regulation, and the downregulated expression of GA catabolism gene (GA2ox2) as the result of feed-forward regulation caused by inhibitor treatment.

Intravenous fluids

1967 ◽  
Vol 5 (4) ◽  
pp. 13-15
Keyword(s):  
Peritoneal Dialysis ◽  
Hospital Pharmacy ◽  
Commercial Production ◽  
Intravenous Fluids ◽  
Operating Theatres ◽  
Increasing Demand

Many hospitals are currently having difficulties in obtaining adequate supplies of i. v. fluids. Staff shortages and increased demand for special sterile bottled solutions for other purposes, for example in operating theatres, for peritoneal dialysis and for haemodialysis, have compelled many hospital pharmacy departments to reduce their output of i. v. fluids. Commercial production of i.v. fluids has not kept pace with the rapidly increasing demand. The quality of i.v. fluids varies considerably:1 pharmacopoeial standards are often not stringently applied, and some of them need revision.

scholarly journals Spectral quality and temporary immersion bioreactor for in vitro multiplication of Eucalytpus grandis × Eucalyptus urophylla

3 Biotech ◽  
2020 ◽  
Vol 10 (10) ◽  
Author(s):  
Denys Matheus Santana Costa Souza ◽  
Maria Lopes Martins Avelar ◽  
Sérgio Bruno Fernandes ◽  
Eduardo Oliveira Silva ◽  
Vinícius Politi Duarte ◽  
...  
Keyword(s):  
Eucalyptus Urophylla ◽  
Spectral Quality ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion ◽  
In Vitro Multiplication

scholarly journals INFLUENCE OF SOME ROOTSTOCK ON THE GROWTH AND QUALITY OF THE VINE PLANTING MATERIAL OF THE MAVRUD VARIETY

2019 ◽  
Vol 17 (4) ◽  
pp. 371-374
Author(s):  
M. Mihaylov
Keyword(s):  
Vitis Vinifera ◽  
19Th Century ◽  
Biological Characteristics ◽  
Vitis Vinifera L ◽  
Red Wines ◽  
Planting Material ◽  
The 19Th Century

The direction related with production of vine planting material originated from the 19th century, after the advent of Phylloxera vastatrix F. in which a large part of the vineyards in Bulgaria and the main wine-growing countries were destroyed. Only solution was proposed for grafting vine varieties from Vitis vinifera L. on resistant rootstocks. A number of biological characteristics of the vine influence both the nursery for scions and the rootstock nursery. One of them, which is main is the affinity of different rootstocks on the different vine varieties. In the southern Bulgaria, where the Mavrud variety is characteristic, high losses are observed due to the low yield of first-class vines. In Bulgaria, the Mavrud variety is highly valued due to its biological qualities, resulting in extracts and dense red wines that have glorified our country.

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