Gut Microbiota: Effect of Pubertal Status

Abstract Background: The make-up of gut microbiota at different puberty stages has not been reported. This cross-sectional study analyzed the bio-diversity of gut microbiota at different puberty stages. Methods: The fecal microbiome was determined in 89 Chinese subjects aged 5-15 years. Subjects were grouped as non-pubertal (n=42) or pubertal (n=47) according to Tanner stages. Gut colonization patterns were determined by 16S rRNA microbiome profiling.Results: The subjects were divided into non-pubertal (n=42, male%: 66.7%) or pubertal groups (n=47, male%:44.68); in both groups, Firmicutes, Bacteroidetes and Proteobacteria were the dominant phylum. There was no difference of alpha- and beta-diversity among disparate puberty stages. Non-pubertal subjects had members of the order Clostridiales, family Clostridiaceae, genus Coprobacillus which were significantly more prevalent than puberty subjects. Also, the pubertal subjects had members of class Betaproteobacteria, order Burkholderiales which were significantly more prevalent than the non-pubertal subjects. Their relative abundance were independent of BMI-Z. In the pubertal subjects, the abundance of genus Adlercreutzia, Ruminococcus, Dorea, Clostridium and Parabacteroides was associated with the level of testosterone.Conclusion: This is the first report of the diversity of gut microbiota at different puberty stages. The various species of gut microbiota changed gradually associated with puberty stages. Differences in gut microflora at different pubertal status may be related to androgen levels.

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Gut Microbiota: Effect of Pubertal Status

10.21203/rs.3.rs-49000/v1 ◽

2020 ◽

Author(s):

Xin Yuan ◽

Ruimin Chen ◽

Ying Zhang ◽

Xiangquan Lin ◽

Xiaohong Yang

Keyword(s):

Gut Microbiota ◽

Cross Sectional Study ◽

Cross Sectional ◽

Pubertal Status ◽

Fecal Microbiome ◽

Alpha And Beta Diversity ◽

Gut Colonization ◽

Puberty Stages ◽

Colonization Patterns ◽

Chinese Subjects

Abstract Background: The make-up of gut microbiota at different puberty stages has not been reported. This cross-sectional study analyzed the bio-diversity of gut microbiota at different puberty stages. Methods: The fecal microbiome was determined in 89 Chinese subjects aged 5-15 years. Subjects were grouped as non-pubertal (n=42) or pubertal (n=47) according to Tanner stages. Gut colonization patterns were determined by 16S rDNA microbiome profiling.Results: The subjects were divided into non-pubertal (n=42, male%: 66.7%) or pubertal groups (n=47, male%:44.68); in both groups, Firmicutes, Bacteroidetes and Proteobacteria were the dominant phylum. There was no difference of alpha- and beta-diversity among disparate puberty stages. Non-pubertal subjects had significantly higher members of the genus Turicibacter and lower members of genus Sutterella than pubertal subjects. Of note, the proportion of genus Sutterella increased gradually with the pubertal status and independent of BMI-Z. In the pubertal subjects, the abundance of genus Adlercreutzia, Dorea, Clostridium and Parabacteroides was associated with the level of testosterone.Conclusion: This is the first report of the diversity of gut microbiota at different puberty stages. The various species of gut microbiota changed gradually associated with puberty stages. Differences in gut microflora at different pubertal status may be related to androgen levels.

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Gut Microbiota: Effect of Pubertal Status

10.21203/rs.3.rs-52945/v1 ◽

2020 ◽

Author(s):

Xin Yuan ◽

Ruimin Chen ◽

Ying Zhang ◽

Xiangquan Lin ◽

Xiaohong Yang

Keyword(s):

Gut Microbiota ◽

Cross Sectional Study ◽

Cross Sectional ◽

Pubertal Status ◽

Fecal Microbiome ◽

Alpha And Beta Diversity ◽

Gut Colonization ◽

Puberty Stages ◽

Colonization Patterns ◽

Chinese Subjects

Abstract Background: The make-up of gut microbiota at different puberty stages has not been reported. This cross-sectional study analyzed the bio-diversity of gut microbiota at different puberty stages. Methods: The fecal microbiome was determined in 89 Chinese subjects aged 5-15 years. Subjects were grouped as non-pubertal (n=42) or pubertal (n=47) according to Tanner stages. Gut colonization patterns were determined by 16S rDNA microbiome profiling.Results: The subjects were divided into non-pubertal (n=42, male%: 66.7%) or pubertal groups (n=47, male%:44.68); in both groups, Firmicutes, Bacteroidetes and Proteobacteria were the dominant phylum. There was no difference of alpha- and beta-diversity among disparate puberty stages. Non-pubertal subjects had significantly higher members of the genus Turicibacter and lower members of genus Sutterella than pubertal subjects. Of note, the proportion of genus Sutterella increased gradually with the pubertal status and independent of BMI-Z. In the pubertal subjects, the abundance of genus Adlercreutzia, Dorea, Clostridium and Parabacteroides was associated with the level of testosterone.Conclusion: This is the first report of the diversity of gut microbiota at different puberty stages. The various species of gut microbiota changed gradually associated with puberty stages. Differences in gut microflora at different pubertal status may be related to androgen levels.

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Gut microbiota: effect of pubertal status

BMC Microbiology ◽

10.1186/s12866-020-02021-0 ◽

2020 ◽

Vol 20 (1) ◽

Author(s):

Xin Yuan ◽

Ruimin Chen ◽

Ying Zhang ◽

Xiangquan Lin ◽

Xiaohong Yang

Keyword(s):

Gut Microbiota ◽

Relative Abundance ◽

Cross Sectional Study ◽

Gut Microflora ◽

Sectional Study ◽

Cross Sectional ◽

Pubertal Status ◽

Alpha And Beta Diversity ◽

Puberty Stages ◽

Androgen Levels

Abstract Background The make-up of gut microbiota at different puberty stages has not been reported. This cross-sectional study analyzed the bio-diversity of gut microbiota at different puberty stages. Result The subjects (aged 5–15 years) were divided into non-pubertal (n = 42, male%: 66.7%) or pubertal groups (n = 47, male%:44.68); in both groups, Firmicutes, Bacteroidetes and Proteobacteria were the dominant phylum. There was no difference of alpha- and beta-diversity among disparate puberty stages. Non-pubertal subjects had members of the order Clostridiales, family Clostridiaceae, genus Coprobacillus which were significantly more prevalent than puberty subjects. Also, the pubertal subjects had members of class Betaproteobacteria, order Burkholderiales which were significantly more prevalent than the non-pubertal subjects. Their relative abundance was independent of BMI-Z. In the pubertal subjects, the abundance of genus Adlercreutzia, Ruminococcus, Dorea, Clostridium and Parabacteroides was associated with the level of testosterone. Conclusions This is the first report of the diversity of gut microbiota at different puberty stages. The various species of gut microbiota changed gradually associated with puberty stages. Differences in gut microflora at different pubertal status may be related to androgen levels.

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Gut Microbiota: Effect of Pubertal Status

10.21203/rs.3.rs-52945/v3 ◽

2020 ◽

Author(s):

Xin Yuan ◽

Ruimin Chen ◽

Ying Zhang ◽

Xiangquan Lin ◽

Xiaohong Yang

Keyword(s):

Gut Microbiota ◽

Relative Abundance ◽

Cross Sectional Study ◽

Gut Microflora ◽

Sectional Study ◽

Cross Sectional ◽

Pubertal Status ◽

Alpha And Beta Diversity ◽

Puberty Stages ◽

Androgen Levels

Abstract Background: The make-up of gut microbiota at different puberty stages has not been reported. This cross-sectional study analyzed the bio-diversity of gut microbiota at different puberty stages. Result: The subjects (aged 5-15 years) were divided into non-pubertal (n=42, male%: 66.7%) or pubertal groups (n=47, male%:44.68); in both groups, Firmicutes, Bacteroidetes and Proteobacteria were the dominant phylum. There was no difference of alpha- and beta-diversity among disparate puberty stages. Non-pubertal subjects had members of the order Clostridiales, family Clostridiaceae, genus Coprobacillus which were significantly more prevalent than puberty subjects. Also, the pubertal subjects had members of class Betaproteobacteria, order Burkholderiales which were significantly more prevalent than the non-pubertal subjects. Their relative abundance was independent of BMI-Z. In the pubertal subjects, the abundance of genus Adlercreutzia, Ruminococcus, Dorea, Clostridium and Parabacteroides was associated with the level of testosterone.Conclusions: This is the first report of the diversity of gut microbiota at different puberty stages. The various species of gut microbiota changed gradually associated with puberty stages. Differences in gut microflora at different pubertal status may be related to androgen levels.

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Gut Microbiota: Effect of Pubertal Status

10.21203/rs.3.rs-52945/v4 ◽

2021 ◽

Author(s):

Xin Yuan ◽

Ruimin Chen ◽

Ying Zhang ◽

Xiangquan Lin ◽

Xiaohong Yang

Keyword(s):

Gut Microbiota ◽

Relative Abundance ◽

Cross Sectional Study ◽

Gut Microflora ◽

Sectional Study ◽

Cross Sectional ◽

Pubertal Status ◽

Alpha And Beta Diversity ◽

Puberty Stages ◽

Androgen Levels

Abstract Background: The make-up of gut microbiota at different puberty stages has not been reported. This cross-sectional study analyzed the bio-diversity of gut microbiota at different puberty stages. Result: The subjects (aged 5-15 years) were divided into non-pubertal (n=42, male%: 66.7%) or pubertal groups (n=47, male%:44.68); in both groups, Firmicutes, Bacteroidetes and Proteobacteria were the dominant phylum. There was no difference of alpha- and beta-diversity among disparate puberty stages. Non-pubertal subjects had members of the order Clostridiales, family Clostridiaceae, genus Coprobacillus which were significantly more prevalent than puberty subjects. Also, the pubertal subjects had members of class Betaproteobacteria, order Burkholderiales which were significantly more prevalent than the non-pubertal subjects. Their relative abundance was independent of BMI-Z. In the pubertal subjects, the abundance of genus Adlercreutzia, Ruminococcus, Dorea, Clostridium and Parabacteroides was associated with the level of testosterone.Conclusions: This is the first report of the diversity of gut microbiota at different puberty stages. The various species of gut microbiota changed gradually associated with puberty stages. Differences in gut microflora at different pubertal status may be related to androgen levels.

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Gut Microbiota Profile and Its Association with Clinical Variables and Dietary Intake in Overweight/Obese and Lean Subjects: A Cross-Sectional Study

Nutrients ◽

10.3390/nu13062032 ◽

2021 ◽

Vol 13 (6) ◽

pp. 2032

Author(s):

Judit Companys ◽

Maria José Gosalbes ◽

Laura Pla-Pagà ◽

Lorena Calderón-Pérez ◽

Elisabet Llauradó ◽

...

Keyword(s):

Gut Microbiota ◽

Dietary Intake ◽

Saturated Fatty Acids ◽

Cross Sectional Study ◽

Sectional Study ◽

Anthropometric Parameters ◽

Cross Sectional ◽

Negatively Associated ◽

Clinical Variables ◽

Obese Subjects

We aimed to differentiate gut microbiota composition of overweight/obese and lean subjects and to determine its association with clinical variables and dietary intake. A cross-sectional study was performed with 96 overweight/obese subjects and 32 lean subjects. Anthropometric parameters were positively associated with Collinsella aerofaciens, Dorea formicigenerans and Dorea longicatena, which had higher abundance the overweight/obese subjects. Moreover, different genera of Lachnospiraceae were negatively associated with body fat, LDL and total cholesterol. Saturated fatty acids (SFAs) were negatively associated with the genus Intestinimonas, a biomarker of the overweight/obese group, whereas SFAs were positively associated with Roseburia, a biomarker for the lean group. In conclusion, Dorea formicigenerans, Dorea longicatena and Collinsella aerofaciens could be considered obesity biomarkers, Lachnospiraceae is associated with lipid cardiovascular risk factors. SFAs exhibited opposite association profiles with butyrate-producing bacteria depending on the BMI. Thus, the relationship between diet and microbiota opens new tools for the management of obesity.

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Altered Gut Microbiota in Irritable Bowel Syndrome and Its Association with Food Components

Journal of Personalized Medicine ◽

10.3390/jpm11010035 ◽

2021 ◽

Vol 11 (1) ◽

pp. 35

Author(s):

Zahra A. Barandouzi ◽

Joochul Lee ◽

Kendra Maas ◽

Angela R. Starkweather ◽

Xiaomei S. Cong

Keyword(s):

Irritable Bowel Syndrome ◽

Gut Microbiota ◽

Alpha Diversity ◽

Diversity Indices ◽

Cross Sectional Study ◽

Caffeine Intake ◽

Caffeine Consumption ◽

Cross Sectional ◽

Food Components ◽

Irritable Bowel

The interplay between diet and gut microbiota has gained interest as a potential contributor in pathophysiology of irritable bowel syndrome (IBS). The purpose of this study was to compare food components and gut microbiota patterns between IBS patients and healthy controls (HC) as well as to explore the associations of food components and microbiota profiles. A cross-sectional study was conducted with 80 young adults with IBS and 21 HC recruited. The food frequency questionnaire was used to measure food components. Fecal samples were collected and profiled by 16S rRNA Illumina sequencing. Food components were similar in both IBS and HC groups, except in caffeine consumption. Higher alpha diversity indices and altered gut microbiota were observed in IBS compared to the HC. A negative correlation existed between total observed species and caffeine intake in the HC, and a positive correlation between alpha diversity indices and dietary fiber in the IBS group. Higher alpha diversity and gut microbiota alteration were found in IBS people who consumed caffeine more than 400 mg/d. Moreover, high microbial diversity and alteration of gut microbiota composition in IBS people with high caffeine consumption may be a clue toward the effects of caffeine on the gut microbiome pattern, which warrants further study.

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