The Characteristics of Human Papillomavirus Infection in Women with Vaginitis and the Correlation with Vaginitis

Abstract Background: Human papillomavirus (HPV) infection is the main cause of cervical cancer in women and vaginitis may affect HPV infection. In order to determine the characteristics as well as the relationship between HPV Infection and vaginitis, we retrospectively collected and analyzed the data from our hospital.Methods: We retrospectively collected the data of 1330 women who underwent twenty-three HPV DNA quantitative measurement and vaginal microecology examination at the same visit from May 2019 to April 2020 in our hospital.Results: The vaginal discharge infection rate was 37.67% in all cases. The total HPV infection rate in women with vaginitis was 39.60% and HPV58, 52, and 16 had the higher prevalence among 23 HPV types, with infection rates of 10.34%, 6.32% and 6.11% respectively. Single-type infection (77.26%) was more common than multiple-type infection (32%).There appeared the same trend in HPV positive cases and vaginitis, reaching the peak in women aged 31–40 years. Among four types of vaginitis, the HPV infection rates were 45.83%, 40.54%, 37.04% and 30.43% in patients with trichomonas vaginitis(TV) , vulvovaginal candidiasis(VVC), intermediate bacterial vaginitis(IBV)and bacterial vaginitis(BV),respectively. Only26.17% women were infected with HPV in patients with normal vaginal micrabiota. Significant differences were found in HPV infection rate between normal vaginal micrabiota group and different vaginitis (P<0.05). There was a correlation between HPV infection and vaginitis(χ2=31.966,P<0.05；r=0.114, P<0.05).Furthermore, patients with BV showed a higher correlation with HPV infection when compared with other groups (r=0.537, P<0.05).Conclusions: This retrospective study demonstrated that HPV58, 52, and 16 are the most common infection subtypes. Our data provide valuable information for HPV-based screening and prevention strategies for women in vaginitis. The single HPV infection was still very common. There was a correlation between HPV infection and vaginitis and patients with BV showed a higher correlation with HPV infection.

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The relationship between cervical human papillomavirus infection and apoptosis

Clinical & Investigative Medicine ◽

10.25011/cim.v31i3.3474 ◽

2008 ◽

Vol 31 (3) ◽

pp. 168 ◽

Cited By ~ 2

Author(s):

Isil Fidan ◽

Gulendam Bozdayi ◽

Seyyal Rota ◽

Aydan Biri ◽

Feryal Çetin Gurelik ◽

...

Keyword(s):

Human Papillomavirus ◽

Viral Infections ◽

Annexin V ◽

Hpv Infection ◽

Hpv Dna ◽

Papillomavirus Infection ◽

Staining Methods ◽

The Relationship ◽

Cervical Human Papillomavirus ◽

Cellular Immortality

Purpose: Cervical carcinoma is the second most common cancer among women worldwide. Viral infections, especially human papillomavirus (HPV) infections, are important factors in its etiology. Changes in apoptotic regulation are considered to have an important role in the carcinogenesis development. In this study, the relationship between apoptosis and HPV infection was investigated. Methods: HPV DNA and HPV DNA type 16 positivity were detected in 110 cervical smear samples with Real Time PCR and sequencing was performed for HPV DNA type 18. The presence of apoptosis was investigated using TUNEL and Annexin V staining methods and analyzed by fluorescence microscope and flow cytometry. Results: HPV DNA type 16 was detected in 9 samples (8.1%), HPV DNA type 18 positive in 6 samples (5.4%) and HPV types other than HPV type 16 and HPV type 18 in 9 samples (8.1%). A decrease apoptosis was found in HPV DNA positive samples compared with controls (P < 0.05). Conclusion: The decrease of apoptosis during HPV infection might cause cellular immortality and then malignant transformation.

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Traditional Chinese Medicine Combined with Routine Nursing on Patients with Cervical Human Papillomavirus Infection

Tobacco Regulatory Science ◽

10.18001/trs.7.6.115 ◽

2021 ◽

Vol 7 (6) ◽

pp. 6415-6421

Author(s):

Jing Ye ◽

Hui Cheng

Keyword(s):

Human Papillomavirus ◽

Chinese Medicine ◽

Traditional Chinese Medicine ◽

Infection Rate ◽

Hpv Infection ◽

Common Disease ◽

Postoperative Rehabilitation ◽

Human Papillomavirus Infection ◽

Papillomavirus Infection ◽

Cervical Human Papillomavirus

Cervical papillomavirus infection is a common disease in women. The risk of permanent infection is the main reason for cervical HPV and its advanced acanthosis. The last time, the incidence rate of cervical papillomavirus is only different from that of malignant tumor women. The trend of young tumors is more and more obvious. Therefore, the treatment of parasitic lesions is very important to prevent or reduce the occurrence of cervical HPV. This paper aims to study the effect of traditional Chinese medicine combined with routine nursing on patients with cervical human papillomavirus infection. The application of traditional Chinese medicine therapy to promote the postoperative rehabilitation of patients with cervical human papillomavirus infection has significant effect, which is of great significance for clinicians in the future work. In this paper, through the introduction of traditional Chinese medicine and traditional Chinese medicine oral and external use combined with routine nursing methods, through the follow-up experimental investigation method to study the cervical human papillomavirus infection rate and the effect of cervical human papillomavirus infection patients after operation, it is proved that the effect of traditional Chinese medicine oral and external use combined with routine nursing on cervical human papillomavirus infection patients is better. The results show that 54% of the cervical HPV infection rate makes people pay more and more attention to cervical HPV. The infection investigation and genotyping can effectively judge the prognosis of patients, which has extremely important clinical significance.

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Characteristics of Human Papillomavirus Infection Among Women With Cervical Cytological Abnormalities in the Zhoupu District, Shanghai City, China, 2014–2019

10.21203/rs.3.rs-51651/v1 ◽

2020 ◽

Author(s):

Ping Li ◽

Qing Liu ◽

Wei Li ◽

Zhou Liu ◽

Baoling Xing ◽

...

Keyword(s):

Cervical Cancer ◽

Human Papillomavirus ◽

Precancerous Lesions ◽

Hpv Infection ◽

Single Type ◽

Squamous Intraepithelial Lesions ◽

Shanghai City ◽

Intraepithelial Lesions ◽

Type Infection ◽

Multiple Type

Abstract Background: Human papillomavirus (HPV) infection is the main cause of precancerous lesions and cervical cancer in women. To determine the epidemiological characteristics as well as the relationship between the HPV genotype and cytology test results among women, we retrospectively collected and analyzed the data from Zhoupu District hospital in Shanghai, China.Methods: We made a retrospective analysis of human papillomavirus prevalence rate of 23,724 women between 2014 and 2019 in the District Zhoupu of Shanghai City in China. Their cervical exfoliations were collected. HPV genotype testing was performed using a commercial kit designed to detect 21 HPV subtypes including 15 high-risk HPV subtypes(16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66 ,68) and 6 low-risk HPV subtypes(6, 11, 42, 43, 44 and 81). And the thinPrep cytological test (TCT) was also performed at the same time.Results: Among all 23,724 cases, 3,816 (16.08%) women were infected with HPV. HPV52 (3.19%), HPV58 (2.47%) and HPV16 (2.34%) had higher prevalence. 3,480(91.20%) single-type infections were more common than 336(8.8%) multiple-type infections. Single-type infection was more frequently seen in women aged 50–60 years (16.63%) and <30 years (15.37%), and multiple-type infection was more common in those aged >= 60 (2.67%). Significant differences in secular trends from 2014 to 2019 were observed for subtypes HPV52, 58 and 16.HPV positive rates of women changed significantly along with the time period from 2014 to 2019.Among 4,502 TCT positive women, 15 (4.04%), 125 (2.64%) ,159 (1.54%), 4,202(17.71%) and 1(0.004%) had atypical squamous cells (ASC), high-grade squamous intraepithelial lesions (HSIL), low-grade squamous intraepithelial lesions (LSIL), atypical glandular cells (AGC) and cervical adenocarcinoma respectively. The HPV infection rates were 66.08%, 63.99%, 115.20%, 119.50%, and 31.72% for NILM, AGCs, HSILs LSILs and ASCs, respectively.Conclusions: HPV and TCT screening were a key step in the secondary prevention of cervical cancer. Further tracking the results of HPV and TCT was an important clinical strategy for the treatment of cervical precancerous lesions. The widespread use of preventive HPV vaccines can significantly reduce the incidence of pre-neoplastic and neoplastic cervical lesions.

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The genomic distribution map of human papillomavirus in China

10.21203/rs.3.rs-155490/v1 ◽

2021 ◽

Author(s):

Ling Chen ◽

Yan Dong ◽

Jiao Li ◽

Jinqiu Zhao ◽

Li Xu ◽

...

Keyword(s):

Human Papillomavirus ◽

High Risk ◽

Infection Rate ◽

Subgroup Analysis ◽

Hpv Infection ◽

Single Type ◽

Distribution Map ◽

Double Infection ◽

Genomic Distribution ◽

High Risk Hpv

Abstract In this study, a total of 301,880 woman were recruited from 4 different regions of China. The overall prevalence of HPV was 18.01 %. The high-risk HPV infection rate was 79.14%, the low-risk HPV infection rate was 12.56 %, and the mixed HPV infection rate was 8.30%. The most common 4 HR HPV subtypes were HPV-52, 16, 58 and 53, which accounted for 20.49 %, 19.93 %, 14.54 % and 10.01 %. In LR HPV genotype, HPV-6 ranked the highest (28.17 %), followed by HPV-81 (9.09 %) and HPV-11 (3.78 %). HPV genotype subgroup analysis also showed that single-type infections had the highest prevalence rate (77.26 %) among HPV positive individuals. Among muti-infection genotype, double infection was the most common with frequencies of 76.04 %. This large report showed that the overall prevalence of HPV was high in China, whose distribution exhibits different patterns across different particular age and regions. Viral genotypes HPV 53, 6 were frequently detected in this population, which is worth of significant clinical attention.

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Detection of Human Papillomavirus Infection in Diseased and Nondiseased Sites of the Respiratory Tract in Recurrent Respiratory Papillomatosis Patients by DNA Hybridization

Annals of Otology Rhinology & Laryngology ◽

10.1177/000348949210100506 ◽

1992 ◽

Vol 101 (5) ◽

pp. 408-412 ◽

Cited By ~ 38

Author(s):

Shirley Pignatari ◽

Elaine M. Smith ◽

Cynthia Shive ◽

Steven D. Gray ◽

Lubomir P. Turek

Keyword(s):

Human Papillomavirus ◽

Respiratory Tract ◽

Dna Hybridization ◽

Hpv Infection ◽

Recurrent Respiratory Papillomatosis ◽

Hpv Dna ◽

Clinically Normal ◽

Papillomavirus Infection ◽

Hybridization Procedure ◽

Cervical Trachea

We examined 12 recurrent respiratory papillomatosis patients, who were undergoing treatment for recurrence of their disease, for the presence of human papillomavirus (HPV). Biopsies were obtained from their respiratory papillomas and nondiseased sites (NDS) of the respiratory tract: the nasopharynx, posterior tonsillar pillar, aryepiglottic fold, cervical trachea, intrathoracic trachea, and bronchi. The presence of HPV DNA was determined by using the ViraPap/ViraType DNA hybridization procedure. Two thirds of the patients were infected with HPV 6/11 in either the diseased papilloma or NDS: 50% of patients with papilloma specimens typed positive (6 of 12), and 40% of the patients typed HPV-positive in one or more biopsies from the NDS (4 of 10). No single NDS was more likely to be infected with HPV than any other. No oncogenic HPV types 16/18 or 31/33/35 were detected in the papillomas or NDS of these patients. Only patients with multiple, and not isolated, papilloma involvement were found to harbor HPV DNA in NDS; and 80 % of those with infected NDS, compared to 20 % of those without infected NDS, required a shorter (no more than 3 months) surgical treatment interval. Our results show that HPV infection frequently persists in adjacent, clinically normal sites, and suggest that the extent of NDS involvement may predict both the extent of disease and the likelihood of recurrence.

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Epidemiologic Profile of Type-Specific Human Papillomavirus Infection after Initiation of HPV Vaccination

Vaccines ◽

10.3390/vaccines8030425 ◽

2020 ◽

Vol 8 (3) ◽

pp. 425

Author(s):

Masayuki Sekine ◽

Manako Yamaguchi ◽

Risa Kudo ◽

Sharon J. B. Hanley ◽

Megumi Hara ◽

...

Keyword(s):

Human Papillomavirus ◽

Hpv Vaccination ◽

Pap Test ◽

Hpv Infection ◽

Infection Rates ◽

Human Papillomavirus Vaccination ◽

Papillomavirus Infection ◽

Vaccination Recommendation ◽

Epidemiologic Profile ◽

The Government

Organized human papillomavirus vaccination (OHPV) in Japan was introduced in 2010 for girls aged 12–16 years who were born in 1994 or later. The rate of OHPV coverage was 70–80%. However, after suspension of the government vaccination recommendation, the coverage dramatically decreased. We aim to investigate the change in prevalence of HPV infection after the initiation of HPV vaccination. We recruited females aged 20–21 years attending public cervical cancer screening from 2014 to 2017 fiscal years (April 2014 to March 2018). Residual Pap test specimens were collected for HPV testing. We compared the prevalence of HPV type-specific infection between women registered in 2014 (born in 1993–1994, including the pre-OHPV generation) and registered in 2015–2017 (born in 1994–1997, the OHPV generation). We collected 2379 specimens. The vaccination coverage figures were 30.7%, 86.6%, 88.4% and 93.7% (p < 0.01) from 2014 to 2017, respectively. The prevalence of HPV16/18 infection significantly decreased from 1.3% in 2014 to 0% in 2017 (p = 0.02). The three most prevalent types were HPV52, 16 and 56 in 2014, and HPV52, 58 and 56 in 2015–2017, respectively. HPV16 and 33 infection rates decreased. On the other hand, the HPV58 infection rate was obviously increased after OHPV from 0.3% to 2.1%. Our study demonstrates that the prevalence of HPV16/18 infection dramatically decreased and the profile of type-specific HPV infection was changed after OHPV.

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Human papillomavirus infection in nasal polyps in a Chinese population

Journal of General Virology ◽

10.1099/vir.0.031955-0 ◽

2011 ◽

Vol 92 (8) ◽

pp. 1795-1799 ◽

Cited By ~ 12

Author(s):

Fei Pei ◽

Xiao-Ping Chen ◽

Yi Zhang ◽

Yan Wang ◽

Qun Chen ◽

...

Keyword(s):

Human Papillomavirus ◽

Nasal Polyps ◽

Hpv Infection ◽

Clinicopathological Characteristics ◽

Low Risk ◽

Future Research ◽

Genotype Distribution ◽

Healthy Controls ◽

Hpv Dna ◽

Papillomavirus Infection

In order to determine the prevalence and genotype distribution of human papillomavirus (HPV) infection in patients with nasal polyps, a total of 204 patients with nasal polyps and 36 healthy controls were recruited for this study. Genomic DNA was extracted from paraffin-embedded tissue sections. HPV DNA genotyping was achieved by a flow-through hybridization and gene-chip method. HPV-positive infection was identified in 82 of 204 (40.2 %) patients, while HPV DNA was not found in healthy controls (P<0.05). Genotyping analysis showed that low-risk HPV genotype 11 was the most prevalent type of HPV in nasal polyps (45.28 %). Both single and multiple HPV genotype infections were found in these HPV-positive cases, although most (74.39 %) were infected with a single genotype. In addition, there was no correlation between HPV infection or HPV subtypes and the clinicopathological characteristics of patients, such as age, gender, number of surgery and disease course. The data from our study clearly demonstrated that HPV infection was associated with nasal polyps. Both high-risk HPV and low-risk HPV (LR-HPV) genotypes were identified in nasal polyp tissues, and LR-HPV-11 was the most prevalent type. Future research will explore the association of HPV infection with the development and progression of nasal polyps.

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Prevalence and risk factors for oral human papillomavirus infection in Mexican HIV-infected men

Salud Pública de México ◽

10.21149/9834 ◽

2018 ◽

Vol 60 (6, nov-dic) ◽

pp. 653

Author(s):

Yuria Ablanedo-Terrazas ◽

Karla Romero-Mora ◽

María Gómez-Palacio ◽

Claudia Alvarado-de la Barrera ◽

Matilde Ruiz-Cruz ◽

...

Keyword(s):

Risk Factors ◽

Human Papillomavirus ◽

Hpv Infection ◽

Oral Infection ◽

Behavioral Risk ◽

Hiv Diagnosis ◽

Hpv Dna ◽

Papillomavirus Infection ◽

Immunodeficiency Virus ◽

Oral Hpv

Objective. To determine the prevalence and risk factors for oral high-risk human papillomavirus (HR-HPV) infection in human immunodeficiency virus (HIV)-infected men. Materials and methods. Consecutive male outpatients with HIV-infection were enrolled. Demographic and behavioral risk data were obtained. Anal swabs and oral rinses were tested for HR-HPV DNA. Oral, pharyngeal and video laryngoscopy examinations were performed for detection of lesions. Results. The prevalence of HR-HPV oral infection was 9.3% (subtypes other than HR HPV 16/18 predominated). The prevalence of anal HR-HPV infection was 75.7%. The risk factors for oral infection with HR-HPV were tonsillectomy (OR=13.12) and years from HIV diagnosis (OR=1.17). Conclusions. Tonsillectomy and years from HIV diagnosis were associated with oral HPV infection. No association was found between oral and anal HR-HPV infections. This is the first study reporting the prevalence and risk factors for oral HR-HPV infection in Mexican HIV-infected population.

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Single-Center Analysis of Human Papillomavirus Infection and P16INK4A Expression among Korean Patients with Penile Cancer

BioMed Research International ◽

10.1155/2019/6940582 ◽

2019 ◽

Vol 2019 ◽

pp. 1-7

Author(s):

Whi-An Kwon ◽

Min Young Yoon ◽

Sung Han Kim ◽

Ho Kyung Seo ◽

Jinsoo Chung ◽

...

Keyword(s):

Human Papillomavirus ◽

Penile Cancer ◽

Hpv Infection ◽

Asian Study ◽

Hpv Dna ◽

P16ink4a Expression ◽

Papillomavirus Infection ◽

Hpv Status ◽

The Mean ◽

Expression Status

Purpose. This study aimed to evaluate the statuses of P16INK4A expression and human papillomavirus (HPV) infection among patients with penile cancer at a single Korean institution. Patients and Methods. Fourteen patients with penile cancer at our center were retrospectively identified and their clinicopathological data were analyzed. The patients’ HPV and P16INK4A expression status (a known tumor suppressor protein) were tested using genotyping with a DNA chip assay and immunohistochemical staining, respectively. The results regarding HPV status were compared to those from another Asian study. Results. The mean age at diagnosis was 60 years (range: 34–86 years). The median tumor size was 3.0 cm (range: 0.6–4.7 cm). Ten tumors were located on the penile glans. Five patients tested positive for HPV DNA (5/14, 36%) and all cases involved HPV type 16 (5/5, 100%). Positive expression of P16INK4A was observed in 6 cases (6/14, 43%). Among the HPV positive cases, 80% of cases (4/5) were also positive for P16INK4A. The prevalence of HPV infection in our study (36%) was higher than in a previous Asian study (23%). Conclusions. This is the first study to evaluate the prevalence of HPV infection and P16INK4A expression among patients with penile cancer at a single Korean institution. The prevalence of HPV (36%) was slightly higher than the results from a previous Asian study. Additional multi-center studies are needed to better understand penile cancer in Korea and to identify biomarkers that can determine high-risk cases and predict their prognosis.

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Verucciform Xanthoma of the Penis Not Associated With Human Papillomavirus Infection

Archives of Pathology & Laboratory Medicine ◽

10.5858/2005-129-e62-vxotpn ◽

2005 ◽

Vol 129 (3) ◽

pp. e62-e64 ◽

Cited By ~ 5

Author(s):

Çağatay Erşahin ◽

Anna M. Szpaderska ◽

Kimberly Foreman ◽

Sherri Yong

Keyword(s):

Human Papillomavirus ◽

Hpv Infection ◽

Nested Polymerase Chain Reaction ◽

Hpv Dna ◽

Negative Results ◽

Papillomavirus Infection ◽

Rare Lesion ◽

Hpv Types ◽

Polymerase Chain ◽

Primer Sets

Abstract Verruciform xanthoma (VX) is a rare lesion with a predilection for oral mucosa. Only 16 cases of VX of the penis have been reported. Histologically, VX lesions in different locations are identical; however, the etiology is controversial. Previous studies have reported the presence of human papillomavirus (HPV) in VX of the skin. The purpose of this study was to determine whether HPV is a causative agent in this rare case of VX of the penis. Microscopically, the lesion demonstrated prominent verrucoid squamous hyperplasia with hyperkeratosis, parakeratosis, and acanthosis. Histiocytes, a hallmark of VX, were identified in the elongated dermal papillae. Nested polymerase chain reaction was performed on the DNA with the commonly used primer sets MY9/MY11 and GP5+/GP6+, which identify more than 40 HPV types. The results failed to identify HPV DNA in the sample, although HPV could be readily detected in genomic DNA extracted from paraffin-embedded condyloma acuminatum, a known HPV-associated lesion. Additionally, we tested a VX lesion of the palate for HPV DNA and obtained negative results. Our results indicate that VX can arise without HPV infection and suggest other possible origins may be involved.

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