scholarly journals Comparative Liver Transcriptome Analysis on Kunming mice with Streptozotocin and Natural Food Induced Type 2 Diabetes Mellitus

2021 ◽  
Author(s):  
YANJUN WU ◽  
XIANGXING ZHU ◽  
Arome Solomon Odiba ◽  
Zisheng LIN ◽  
JIANCONG WEN ◽  
...  
Keyword(s):  
Diabetic Animal ◽  
High Carbohydrate Diet ◽  
Natural Food ◽  
Standard Diet ◽  
High Fat ◽  
Carbohydrate Diet ◽  
Kegg Pathways ◽  
High Carbohydrate ◽  
Liver Transcriptome

Abstract Background: Streptozotocin is a classic drug used to induce diabetic animal models. Objective: The aim of this study is to investigate the liver transcriptome of diabetic Kunming mice induced by streptozotocin (STZ) or Non-STZ. Methods: Forty male mice were randomly assigned into 4 groups, namely Control (Ctr, standard diet), mHH (high fat and high carbohydrate diet), mHS (high fat and high carbohydrate diet for 4 weeks followed by 60 mg/kg STZ for 3 consecutive days) and mSH (60 mg/kg STZ for 3 consecutive days and then fed with high fat and high carbohydrate diet for 12 weeks). All mice injected with STZ were identified as diabetes despite the sequential feeding of high fat and high carbohydrate diet. Results: Only 7 of 13 mice in mHH group met the diagnostic criteria for diabetes. The FBG of mHH, mHS, mSH and Ctrl groups were 13.27 ± 1.14, 15.01 ± 2.59, 15.95 ± 4.38 and 6.28 ± 0.33 mmol/L at 12th week, respectively. Compared with mHH group, the transcriptions of 85 genes were elevated in liver of mHS mice while 21 genes were down-regulated, and 97 genes were shown to be up-regulated in mSH group while 35 genes were decreased. Total 43 co-expressed genes were identified in mHSvsmHH and mSHvsmHH groups. GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) analyses showed that there were 2 corporate GO terms and 2 KEGG pathways significantly annotated in STZ-treated groups. Both the GO term and pathway were related to the metabolism mediated by p53. Conclusion: High fat and high carbohydrate diet combined with low dose of STZ can effectively induce diabetic models in Kunming mice despite the abnormal expressions of genes in the liver. The differentially expressed genes were related to the metabolism mediated by p53.

scholarly journals Mixed lineage kinase 3 deficient mice are protected against the high fat high carbohydrate diet-induced steatohepatitis

2013 ◽  
Vol 34 (3) ◽  
pp. 427-437 ◽  
Author(s):  
Samar H. Ibrahim ◽  
Gregory J. Gores ◽  
Petra Hirsova ◽  
Michelle Kirby ◽  
Lili Miles ◽  
...  
Keyword(s):  
High Carbohydrate Diet ◽  
High Fat ◽  
Carbohydrate Diet ◽  
Mixed Lineage Kinase ◽  
High Carbohydrate ◽  
Mixed Lineage Kinase 3 ◽  
Deficient Mice

scholarly journals Biochemical aspects of malabsorption in marasmus: effect of dietary rehabilitation

1985 ◽  
Vol 54 (3) ◽  
pp. 567-575 ◽  
Author(s):  
H. C. Mehta ◽  
A. S. Saini ◽  
Harjit Singh ◽  
P. S. Dhatt
Keyword(s):  
Total Energy ◽  
High Fat Diet ◽  
High Protein Diet ◽  
High Carbohydrate Diet ◽  
High Protein ◽  
Protein Diet ◽  
Duodenal Juice ◽  
High Fat ◽  
Carbohydrate Diet ◽  
High Carbohydrate

1. Sixty marasmic children were investigated for the absorption of xylose, proteins and fats. Their duodenal juice samples were also analysed for bile salts and microflora.2. The marasmic children were then studied in three groups of twenty by allocating them to three different dietary schedules: a high-protein diet (30% of the total energy from protein), a high-fat diet (40% of the total energy from fat) and a high-carbohydrate diet (70% of the total energy from carbohydrate) for 2 weeks and the previous measurements repeated.3. Whereas the high-fat diet resulted in improved fat absorption, along with an increase in total and conjugated bile acids, and the high-carbohydrate diet led to improved xylose absorption, the diet rich in protein resulted in an improvement in the absorption of all three dietary ingredients. It appears that a high-protein diet improves the overall absorption process by improving the intestinal environment as a whole, while high-carbohydrate and high-fat diets bring about adaptive changes related to the respective absorptive processes.

scholarly journals Restoration of the glycogen-forming function of hepatocytes in rats with liver cirrhosis is facilitated by a high-carbohydrate diet

1999 ◽  
Vol 81 (6) ◽  
pp. 473-480 ◽  
Author(s):  
Margarita V. Kudryavtseva ◽  
Natalia N. Besborodkina ◽  
Boris N. Kudryavtsev
Keyword(s):  
Liver Cirrhosis ◽  
Glycogen Phosphorylase ◽  
Glycogen Synthase ◽  
Normal Liver ◽  
Fold Increase ◽  
Tissue Level ◽  
High Carbohydrate Diet ◽  
Standard Diet ◽  
Carbohydrate Diet ◽  
High Carbohydrate

Using cytofluorimetric and biochemical methods, the content of glycogen and its labile and stable fractions, as well as activities of glucose-6-phosphatase (EC 3.1.3.9), glycogen phosphorylase (EC 2.4.1.1) and glycogen synthase (EC 2.4.1.11) were determined in the rat liver for 6 months after chronic poisoning of the animals with CCl4 and then at 1, 3, and 6 months after the end of the poisoning. One group of rats was given a standard diet, the other, a high-carbohydrate diet. The 6-month long chronic intoxication with CCl4 was shown to produce development of typical liver cirrhosis characterized by a 2·8-fold increase in the total glycogen content in hepatocytes as compared with normal cells, by a fall in the glycogen labile fraction (from 85 to 53 % of the total glycogen) as well as by decreases in the activities of glycogen phosphorylase and glucose-6-phosphatase by 25 and 82% respectively. The structural rehabilitation occurred faster and more completely at the cellular level than at the tissue level. Functional variables of the cirrhotic liver tissue also recovered, after cessation of poisoning, faster and more completely than the liver structure at the tissue level: glycogen levels in hepatocytes fell dramatically, the labile: stable glycogen fraction ratio recovered completely, and the activity of glycogen phosphorylase rose to the level characteristic of the normal liver. Use of the high-carbohydrate diet promoted a somewhat faster and more complete recovery of hepatic structure and function.

scholarly journals P2-190: SOLUBLE TNF MEDIATES HIGH-FAT-HIGH-CARBOHYDRATE DIET-INDUCED ALTERATIONS IN PERIPHERAL AND BRAIN IMMUNOPHENOTYPE IN A MOUSE MODEL OF ALZHEIMER'S DISEASE

2019 ◽  
Vol 15 ◽  
pp. P649-P650
Author(s):  
Lori N. Eidson ◽  
Kathryn P. MacPherson ◽  
Mary K. Herrick ◽  
Maria Elizabeth de Sousa Rodrigues ◽  
Lindsey J. Sniffen ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Mouse Model ◽  
High Carbohydrate Diet ◽  
High Fat ◽  
Carbohydrate Diet ◽  
High Carbohydrate ◽  
Model Of Alzheimer’S Disease

Effects of quercetin on the neuromotor function and behavioral responses of Wistar and Zucker rats fed a high-fat and high-carbohydrate diet

2020 ◽  
Vol 378 ◽  
pp. 112270 ◽  
Author(s):  
Kristina V. Mzhelskaya ◽  
Vladimir A. Shipelin ◽  
Antonina A. Shumakova ◽  
Anna D. Musaeva ◽  
Jorge S. Soto ◽  
...  
Keyword(s):  
Behavioral Responses ◽  
High Carbohydrate Diet ◽  
Zucker Rats ◽  
High Fat ◽  
Carbohydrate Diet ◽  
High Carbohydrate ◽  
Neuromotor Function
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