scholarly journals Sebaceous Carcinoma of The Lip: Report of a Case and Literature Review.

2021 ◽  
Author(s):  
Mariateresa Ambrosino ◽  
pasquale somma ◽  
andrea santarelli ◽  
stefania staiabano ◽  
michele di cosola ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Cell Carcinoma ◽  
Histopathological Examination ◽  
Sebaceous Carcinoma ◽  
Neoplastic Tissue ◽  
Male Smoker ◽  
Neoplastic Cells ◽  
Ki 67 ◽  
Lower Lip ◽  
S 100

Abstract Background Sebaceous carcinoma (SC) is a very rare, aggressive, malignant tumor arising in the adnexal epithelium of the sebaceous gland. SC in the oral cavity is extremely rare, in literature there are only 14 cases. We reported the 4th case of sebaceous carcinoma involving the lip Case presentation A 71-year-old male smoker presented an ulcerated lesion in the lateral region of the lower lip. The patient stated that the lesion had been present for 1 year. The past medical history was unremarkable. Extra-oral examination revealed a markedly ulcerated, exophytic, irregularly shaped, indurated mass of the lower right labial region, measuring 1.8 cm in size. The nodular lesion, located at the point of transition between mucosa and skin, showed a central ulceration. No other intraoral lesions were identified. The clinical differential diagnosis included squamous cell carcinoma, basal cell carcinoma with sebaceous differentiation and salivary gland neoplasms. The operation was performed under local anaesthesia. On histopathological examination, the tumor was composed by nodules or sheet of cells separated by a fibrovascolar stroma. The neoplastic tissue was deeply infiltrating, involving the submucosa and even the underlying muscle. Neoplastic cells showed a range of sebaceous differentiation with finely vacuolated rather than clear cytoplasm. Neoplastic cells were positive for S-100 protein, EMA, but negative for CEA. Therefore, based on these findings, a diagnosis of sebaceous carcinoma of the lower lip was rendered. Conclusion The histogenesis, differential diagnosis, and clinicopathologic conditions of this disease in the literature were reviewed. SC should be distinguished from other tumors full of vacuolated clear cells. Some useful biomarkers can be Ki-67, P53, CK, PAS, S-100, EMA, and AR.

scholarly journals Effects of electrochemotherapy with cisplatin and peritumoral IL-12 gene electrotransfer on canine mast cell tumors: a histopathologic and immunohistochemical study

2017 ◽  
Vol 51 (3) ◽  
pp. 286-294 ◽  
Author(s):  
Claudia Salvadori ◽  
Tanja Svara ◽  
Guido Rocchigiani ◽  
Francesca Millanta ◽  
Darja Pavlin ◽  
...  
Keyword(s):  
Mast Cell ◽  
T Lymphocytes ◽  
Mhc Class Ii ◽  
Cellular Response ◽  
Histopathological Examination ◽  
Combined Treatment ◽  
Gene Electrotransfer ◽  
Neoplastic Cells ◽  
Ki 67 ◽  
Mast Cell Tumors

AbstractBackgroundThe study was aimed to characterize tumor response after combined treatment employing electrochemotherapy with IL-12 gene electrotransfer in dogs with spontaneous mast cell tumors (MCT).Materials and methodsEleven dogs with eleven MCTswere included in the study. Histological changes were investigated in biopsy specimens collected before the treatment (T0), and 4 (T1) and 8 weeks (T2) later. Cellular infiltrates were characterized immunohistochemically by using anti CD3, CD20, Foxp3 (Treg), CD68 and anti MHC-class II antibodies. Proliferation and anti-apoptotic activity of neoplastic cells were assessed using anti Ki-67 and Bcl-2 antibodies. Angiogenetic processes were investigated immunohistochemically by using anti Factor VIII and anti CD31 antibodies and micro vessel density quantification.ResultsHistopathological examination of samples at T0confirmed the diagnosis and the presence of scanty infiltrates consisted mainly of T-lymphocytes and macrophages. At T1and T2neoplastic cells were drastically reduced in 7/11 cases, small clusters of neoplastic cells were detected in 3/11 cases and 1/11 cases neoplastic cells were still evident. Proliferation activity of neoplastic cells was significantly reduced at T1and T2and expression of anti-apoptotic protein at T1. Microvessel density was drastically reduced in all samples after treatment. The number of T-lymphocytes increased at T1, although not significant, while Treg were significant higher at T1and macrophages at T2.ConclusionsThe combined electrochemotherapy and IL-12 gene electrotransfer effectively induced a cellular response against neoplastic cells characterized mainly by the recruitment of T-lymphocytes and macrophages and a fibrotic proliferation with reduction of microvessels.

Immunohistochemical comparative analysis of IMP-3 and KI-67 in actinic cheilitis and lower lip squamous cell carcinoma

2021 ◽  
Author(s):  
Adriana Costa de Souza Martins Câmara ◽  
Amanda Katarinny Goes Gonzaga ◽  
Joabe dos Santos Pereira ◽  
Salomão Israel Monteiro Lourenço Queiroz ◽  
Éricka Janine Dantas da Silveira ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Comparative Analysis ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Actinic Cheilitis ◽  
Ki 67 ◽  
Lower Lip

scholarly journals Malignant Leydig cell tumor in dogs: two cases and a review of the literature

2019 ◽  
Vol 31 (4) ◽  
pp. 557-561 ◽  
Author(s):  
Tomoo Kudo ◽  
Junichi Kamiie ◽  
Naoyuki Aihara ◽  
Masaki Doi ◽  
Ayumi Sumi ◽  
...  
Keyword(s):  
Leydig Cell ◽  
Testicular Tumor ◽  
Cutaneous Metastasis ◽  
Leydig Cell Tumor ◽  
Cell Tumor ◽  
Neoplastic Tissue ◽  
Neoplastic Cells ◽  
Ki 67 ◽  
Primary Tumors ◽  
Eosinophilic Cytoplasm

Malignant Leydig cell tumor (MLCT) is a rare testicular tumor in dogs. We report herein 2 dogs with MLCT and cutaneous metastasis. Grossly, marked enlargement and distortion of the involved testes were noted; on cut surface, the parenchyma was completely replaced by neoplastic tissue. In addition, these tumors had extensive necrosis and hemorrhage. Case 1 had a rapidly growing cutaneous mass in the left angle of the mouth; the lesion was well-circumscribed and had an indistinct lobular pattern. Case 2 had multiple cutaneous masses in the dorsal neck region, the thoracic back region, and the right hindlimb. Microscopically, the tumor lobules were composed of oval-to-polyhedral cells with eosinophilic cytoplasm and resembled testicular tumors. By immunohistochemistry, the neoplastic cells in both the testicular and cutaneous tumors were positive for inhibin-alpha and melan A. The mitotic counts of the primary tumors from cases 1 and 2 were 21 and 11 per 10 high-power fields, respectively. Based on these findings, the cases were diagnosed as MLCT with cutaneous metastasis. Ki-67 expression in the neoplastic cells of the 2 cases was higher than in benign Leydig cell tumors. Our findings may be helpful for the diagnosis of canine MLCT.

Primary Pulmonary Acinic Cell Carcinoma: A Clinicopathological Study of 6 Cases and Literature Review

2019 ◽  
Vol 27 (6) ◽  
pp. 584-592 ◽  
Author(s):  
Ling Nie ◽  
Chunlei Zhou ◽  
Hongyan Wu ◽  
Qiang Zhou ◽  
Fanqing Meng
Keyword(s):  
Cell Carcinoma ◽  
Comprehensive Evaluation ◽  
Clinicopathological Features ◽  
Acinic Cell Carcinoma ◽  
Mitotic Figure ◽  
Neoplastic Cells ◽  
Acinic Cell ◽  
S 100 ◽  
The Difference ◽  
The Right

Primary pulmonary acinic cell carcinoma (ACC) is rare. The clinicopathological features are not identical to that of classic ACC that leads to misdiagnosis. In this article, we summarized the clinicopathological features of 25 such cases, including 6 cases in this series and additional 19 cases in the literature. Pulmonary ACCs showed an overwhelming solid growth pattern. The neoplastic cells had eosinophilic granular and clear cytoplasm in most cases and displayed basophilic cytoplasm in only 4 cases. Intratumoral fibrous septa, mitotic figure, necrosis, and psammoma bodies were observed in some cases. Prominent nuclear atypia and perineural invasion might suggest high-grade transformation, metastasis, and recurrence. The tumor cells were strongly positive for CK8/18 and negative for TTF-1, p63, S-100, mammaglobin, MUC5b, MUC5ac, and DOG1. CK7 was exclusively positive for neoplastic cells with ductal differentiation. Of the 25 included cases, 10 cases were initially misdiagnosed. The tumor was prone to involve the right bronchus. The patient outcome was favorable. The accurate diagnosis of primary pulmonary ACC relies on comprehensive evaluation of histological and immunohistochemical features and realization of the difference from classic ACC.

scholarly journals Cytokeratin 17 and Ki-67: Immunohistochemical markers for the differential diagnosis of keratoacanthoma and squamous cell carcinoma

2017 ◽  
Vol 13 (4) ◽  
pp. 2539-2548
Author(s):  
Cem Leblebici ◽  
Esra Pasaoglu ◽  
Canan Kelten ◽  
Seher Darakci ◽  
Nevra Dursun
Keyword(s):  
Squamous Cell Carcinoma ◽  
Differential Diagnosis ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Ki 67 ◽  
Cytokeratin 17 ◽  
Immunohistochemical Markers

scholarly journals EXPRESSION OF OCT-4, P53, P16 AND KI-67, WITH HPV ASSOCIATED PRECANCER AND CANCER OF THE CERVIX

2016 ◽  
Vol 7 (1) ◽  
pp. 34-38
Author(s):  
E A Kogan ◽  
Li Ts Ts ◽  
N M Faizullina ◽  
A V Kazachenko
Keyword(s):  
Squamous Cell Carcinoma ◽  
Differential Diagnosis ◽  
Tumor Growth ◽  
Cell Carcinoma ◽  
Tumor Markers ◽  
Hpv Infection ◽  
Ki 67 ◽  
P53 Expression ◽  
Cancer Of The Cervix ◽  
Cancer Spheroids

The pathology of cervical HPV infection is detected by three types of epithelial-mesenchymal spheroidal structures: reparative, neoplastic and cancerous involved in reparation processes and in tumor growth. Cancer spheroids are different from neoplastic not only larger in size but also a tendency to merge, and high expression of p53. Expression of tumor markers p53, p16 and Ki-67 could be used in the early differential diagnosis of precancer and squamous cell carcinoma of the cervix.

scholarly journals Immunoexpression of P16 and Ki 67 in oral squamous cell carcinoma: Association with clinicopathological parameters.

2020 ◽  
Vol 3 (2) ◽  
pp. 135-144
Author(s):  
Shadan Omer ◽  
Payman Rashid
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Biological Behavior ◽  
Clinicopathological Parameters ◽  
Statistical Association ◽  
Ki 67 ◽  
Lower Lip ◽  
Significant Statistical Association

Background and Objectives: Oral squamous cell carcinoma (OSCC) is considered as a major health problem worldwide and has been associated with high recurrence rate and poor progno-sis. Advances in understanding of OSCC have not improved the outcome in their management significantly. Many studies have focused on the roles of biomolecular markers in OSCC. The use of p16 and Ki67 as biomarkers of biological behavior of oral squamous cell carcinoma is contro-versial. This study aimed to determine immunoexpression of P16 and Ki67 in oral squamous cell carcinoma and to evaluate their association with various clinicopathological parameters. Materials and Methods: Fifty cases of squamous cell carcinoma from different locations in the oral cavity were included in this cross sectional study. The cases were collected from Rizgary Teaching Hospital and Private Laboratories in Erbil city during a period of eight months from October 2018 to May 2019. The expression of p16 and Ki 67 were evaluated immunohistochem-ically; the findings were correlated with the age of the patients, gender, site of the tumor and grade of the tumor. Result: A total of 50 patients with oral squamous cell carcinoma were enrolled in this study the age ranged from 33 to 89 years, with a mean age ± SD of (64.24 ±12.01) years and more than half (52.0%) of them were males. Lower lip was the most common site of the tumor followed by upper lip and tongue (42.0%, 26.0% and 18.0%, respectively). Histopathological findings of the tumor showed that (54.0%) of the patients had moderately differentiated squamous cell carci-noma. However, (84.0%) of the patients showed negative expression of P 16, while Ki 67 ex-pression was positive among (76.0%) of them. No significant statistical association were found between immunoexpression of p16 and age, sex of patient, site of the tumor and grade of the tumor (P=0.67, P=0,095, P=0.696, P=0.454 respectively). No significant statistical association were found between immunoexpression of Ki67 and age, sex of patient, site of the tumor and grade of the tumor (P=0.637, P=0,411, P=0.353, P=1.00 respectively). Conclusion: in relation to the results obtained in this study no significant association were found between P16 and Ki 67 immunoexpression in oral squamous cell carcinoma with clinicopatho-logical parameters. Further researches have to be designed to better understand the role of p16 and Ki 67 in OSCC. Keywords: oral squamous cell carcinoma, immunoexpression, P16, Ki67.

scholarly journals Hemangiosarcoma in a Cat’s Tongue

2019 ◽  
Vol 48 ◽  
Author(s):  
Calvin Braga Gnoatto ◽  
Tatiana Regina Lichmann ◽  
Germano Filipe Grings ◽  
Fernanda Genro Cony ◽  
Regina Tose Temper ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Differential Diagnosis ◽  
Smooth Muscle ◽  
Oral Cavity ◽  
Histopathological Examination ◽  
Malignant Neoplasm ◽  
Diagnostic Methods ◽  
Blood Contamination ◽  
Neoplastic Cells ◽  
Vascular Channels

Background: Hemangiosarcoma is a primary malignant neoplasm of vascular endothelial cells, common in dogs and uncommon in cats. It is characterized by being aggressive and presenting high metastatic behavior. It corresponds to 0.5% to 2% of all feline neoplasms, with more reports in shorthaired cats aged between 8 and 12.2 years old, with no predisposition for sex or breed. Among the diagnostic methods, histopathological and immunohistochemical exams stand out because they are more accurate, since the result of fine-needle aspiration biopsy is usually hidden by blood contamination. This report aims to describe a case of a hemangiosarcoma in a cat's tongue.Case: A 9-year-old, female, shorthair cat with a history of weight loss, lethargy, chewing difficulty with the evolution of three months and two-day oral bleeding was attended. Physical examination revealed blood in the oral cavity, enlargement, and darkening of the tongue surface, normalized oral and ocular mucous membranes, estimated dehydration of 7%, a rectal temperature of 38,8°C, and pulmonary and cardiac auscultation without changes. The cat was hospitalized, and euthanasia was elected. Cytological and histopathological exams of the tongue were requested, which revealed a neoplastic proliferation of endothelial cells expanding and replacing the muscular layer of the tongue, forming primitive vascular structures filled with a large number of red blood cells and supported by a delicate fibrovascular stroma. There were moderate anisocytosis and anisocariosis and rare figures of mitosis. Immunohistochemical examination showed the expression of von Willebrand factor, smooth muscle vimentin, and actin in the cytoplasm of neoplastic cells, and negative staining for pancitokeratin.Discussion: According to the results of cytological, histopathological and immunohistochemical examinations, the cat was diagnosed with tongue hemangiosarcoma. The involvement of the tongue in cases of feline hemangiosarcoma is not commonly reported, as the oral cavity is an uncommon site of neoplasm in cats and hemangiossarcoma neoplasm is considered rare in this species. The cytological examination of the patient's tongue was useful to suggest the diagnosis of sarcoma due to a large number of neoplastic cells present in the sample, and later, because it is more specific, the histopathological examination was performed to find out the origin of the tissue. The definitive diagnosis of hemangiosarcoma could be confirmed by the alterations found in the histopathological and immunohistochemical exams. The appearance of erythrocytes within tumor vascular channels, the rapid mass development, moderate anisocytosis, anisocariosis, and irregularity of vascular channels, along with the expression of smooth muscle action, excluded the differential diagnosis of hemangioma and lymphangiosarcoma. Due to the ability of the hemangiosarcoma to be a primary or metastatic tumor and the performance of the necropsy examination was not authorized, we could not determinate the primary origin of the tumor. However, we suggest in the present study that the neoplasm is primary of the tongue because there are no skin lesions or alterations in other organs, besides the tongue is not a common site of metastasis. In conclusion, hemangiosarcoma should be included as a differential diagnosis in cases of tongue neoplasia, although uncommon in felines, and that histopathological and immunohistochemical examinations are indispensable to define an accurate diagnosis.

scholarly journals Assessment of Density of Neovascularization in Lower Lip Squamous Cell Carcinoma in Relation To Neoplasm Differentiation Grade in Patients with and without Neck Lymph Nodes Metastasis

2019 ◽  
Vol 7 (1) ◽  
pp. 19-23
Author(s):  
Lena Kakasheva-Mazhenkovska ◽  
Marko Kostovski ◽  
Gjorgje Gjokik ◽  
Vesna Janevska
Keyword(s):  
Squamous Cell Carcinoma ◽  
Lymph Nodes ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Histopathological Examination ◽  
Tumour Progression ◽  
Regional Lymph Nodes ◽  
Lower Lip ◽  
Neck Metastasis ◽  
Significant Difference

BACKGROUND: Squamous cell carcinoma (SCC) of the lower lip is a relatively rare carcinoma, with the incidence of 1 to 2%, but it is the most common carcinoma in the oral cavity accounting for 25-30% of all malignant oral tumours. AIM: This study aimed to determine the role of neovascularisation in the process of tumour progression. METHODS: We analysed the surgical specimens obtained from 60 patients with squamous cell carcinoma (SCC) of the lower lip. The examined group consisted of 45 specimens of patients without metastasis and 15 specimens of patients with metastasis in the regional lymph nodes. Histopathological slides were immunohistochemically stained with an antibody against CD34 and by hematoxylin & eosin staining for routine histopathological examination. RESULTS: The results obtained showed a statistically significant difference in the density of neovascularisation between groups of the SCC with different grade of differentiation (Kruskal-Wallis test: H (2, N = 60) = 30.0943, p = 0.00001). Statistical analysis also showed a significant difference in the density of vascularisation of lower lip SCC between patients without metastasis and patients with neck metastasis (Mann-Whitney U test, p = 0.000198). Applying Pearson’s chi-square test, we found a highly significant statistical difference in grade of SCC differentiation in patients with and without neck metastasis (p = 0.0000). CONCLUSION: In conclusion, the density of neoangiogenesis is increased in tumours with poorer differentiation and in patients with neck metastasis. So, the density of neovascularisation of the primary lip SCC may predict the tumour progression.

Sign in / Sign up

Export Citation Format

Share Document