scholarly journals Myogenic differentiation potential of chicken mesenchymal stem cells from bone marrow

2021 ◽  
Author(s):  
Zhen Zhou ◽  
Changbin Zhao ◽  
Bolin Cai ◽  
Manting Ma ◽  
Shaofen Kong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Muscle Development ◽  
Myogenic Differentiation ◽  
Horse Serum ◽  
Growth Characteristic ◽  
Normal Growth ◽  
Receptor Interaction ◽  
Differentiation Potential ◽  
Differential Adhesion

Abstract Background: Mesenchymal stem cells (MSCs) have the potential to multilineage differentiation, which can be used for a good model to provide critical insight of chicken muscle development. Differential adhesion method is one of the commonest methods to isolate MSCs based on the ability of plastic adhesion. 5-azacytidine (5-Aza), dexamethasone (DXMS), hydrocortisone (HC) and horse serum had been proved the potential to induce the myogenic differentiation of MSCs. However, the myogenic differentiation of MSCs is still poorly understood in chicken. In present study, we isolated chicken mesenchymal stem cells (cMSCs) from bone using 4-hour differential adhesion method and analyzed the myogenic effect of cMSCs treated with different method based on 5-Aza, DXMS, HC and horse serum.Results: cMSCs isolated by 4-hour differential adhesion method expressed MSCs special surface markers and presented normal growth characteristic. cMSCs showed great potential of myogenic differentiation by the treatment of 5-Aza and horse serum. RNA-sequence, GO and KGEE enrichment analysis revealed that this effect might be based on demethylation of 5-Aza and ECM-receptor interaction, focal adhesion, PI3K-Akt, p53, TGF-beta signaling pathways. Moreover, DXMS, HC and horse serum also presented potential of myogenic differentiation, but the effect was not as good as 5-Aza and horse serum method.Conclusions: cMSCs showed potential of myogenic differentiation by the treatment of 5-Aza and horse serum or DXMS, HC and horse serum.

Effect of Substrate Elasticity on In Vitro Aging of Human Mesenchymal Stem Cells

2012 ◽  
Vol 1498 ◽  
pp. 39-45
Author(s):  
Courtney E. LeBlon ◽  
Caitlin R. Fodor ◽  
Tony Zhang ◽  
Xiaohui Zhang ◽  
Sabrina S. Jedlicka
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Elastic Modulus ◽  
Myogenic Differentiation ◽  
Human Mesenchymal Stem Cells ◽  
Immunofluorescent Staining ◽  
Differentiation Potential ◽  
Gene And Protein Expression ◽  
The Impact

ABSTRACTHuman mesenchymal stem cells (hMSCs) were routinely cultured on tissue-culture polystyrene (TCPS) to investigate the in vitro aging and cell stiffening. hMSCs were also cultured on thermoplastic polyurethane (TPU), which is a biocompatible polymer with an elastic modulus of approximately 12.9MPa, to investigate the impact of substrate elastic modulus on cell stiffening and differentiation potential. Cells were passaged over several generations on each material. At each passage, cells were subjected to osteogenic and myogenic differentiation. Local cell elastic modulus was measured at every passage using atomic force microscopy (AFM) indentation. Gene and protein expression was examined using qRT-PCR and immunofluorescent staining, respectively, for osteogenic and myogenic markers. Results show that the success of myogenic differentiation is highly reliant on the elastic modulus of the undifferentiated cells. The success of osteogenic differentiations is most likely somewhat dependent on the cell elastic modulus, as differentiations were more successful in earlier passages, when cells were softer.

scholarly journals Effect of 20(S)-Hydroxycholesterol on Multilineage Differentiation of Mesenchymal Stem Cells Isolated from Compact Bones in Chicken

Genes ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 1360
Author(s):  
Roshan Adhikari ◽  
Chongxiao Chen ◽  
Woo Kyun Kim
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Health ◽  
Hedgehog Signaling ◽  
Myogenic Differentiation ◽  
Adipogenic Differentiation ◽  
Bioactive Molecules ◽  
Marker Genes ◽  
Human Mscs ◽  
Differentiation Potential

Bone health and body weight gain have significant economic and welfare importance in the poultry industry. Mesenchymal stem cells (MSCs) are common progenitors of different cell lineages such as osteoblasts, adipocytes, and myocytes. Specific oxysterols have shown to be pro-osteogenic and anti-adipogenic in mouse and human MSCs. To determine the effect of 20(S)-hydroxycholesterol (20S) on osteogenic, adipogenic, and myogenic differentiation in chicken, mesenchymal stem cells isolated from compact bones of broiler chickens (cBMSCs) were subjected to various doses of 20S, and markers of lineage-specific mRNA were analyzed using real-time PCR and cell cytochemistry. Further studies were conducted to evaluate the molecular mechanisms involved in lineage-specific differentiation pathways. Like human and mouse MSCs, 20S oxysterol expressed pro-osteogenic, pro-myogenic, and anti-adipogenic differentiation potential in cBMSCs. Moreover, 20(S)-Hydroxycholesterol induced markers of osteogenic genes and myogenic regulatory factors when exposed to cBMSCs treated with their specific medium. In contrast, 20S oxysterol suppressed expression of adipogenic marker genes when exposed to cBMSCs treated with OA, an adipogenic precursor of cBMSCs. To elucidate the molecular mechanism by which 20S exerts its differentiation potential in all three lineages, we focused on the hedgehog signaling pathway. The hedgehog inhibitor, cyclopamine, completely reversed the effect of 20S induced expression of osteogenic and anti-adipogenic mRNA. However, there was no change in the mRNA expression of myogenic genes. The results showed that 20S oxysterol promotes osteogenic and myogenic differentiation and decreases adipocyte differentiation of cBMSCs. This study also showed that the induction of osteogenesis and adipogenesis inhibition in cBMSCs by 20S is mediated through the hedgehog signaling mechanism. The results indicated that 20(S) could play an important role in the differentiation of chicken-derived MSCs and provided the theory basis on developing an intervention strategy to regulate skeletal, myogenic, and adipogenic differentiation in chicken, which will contribute to improving chicken bone health and meat quality. The current results provide the rationale for the further study of regulatory mechanisms of bioactive molecules on the differentiation of MSCs in chicken, which can help to address skeletal health problems in poultry.

scholarly journals Myogenic Differentiation Potential of Mesenchymal Stem Cells Derived from Fetal Bovine Bone Marrow

2017 ◽  
Vol 29 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Lucas Hidenori Okamura ◽  
Paloma Cordero ◽  
Jaime Palomino ◽  
Victor Hugo Parraguez ◽  
Cristian Gabriel Torres ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Myogenic Differentiation ◽  
Bovine Bone ◽  
Differentiation Potential ◽  
Fetal Bovine

scholarly journals Umbilical cord tissue is a robust source for mesenchymal stem cells with enhanced myogenic differentiation potential compared to cord blood

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Shivangi Mishra ◽  
Jayesh Kumar Sevak ◽  
Anamica Das ◽  
G. Aneeshkumar Arimbasseri ◽  
Shinjini Bhatnagar ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Myogenic Differentiation ◽  
Differentiation Potential ◽  
Transcriptomic Sequencing ◽  
Differentiation Protocol ◽  
Umbilical Cord Tissue ◽  
Term Births

Abstract Differentiation of mesenchymal stem cells (MSCs) derived from two different sources of fetal tissues such as umbilical cord blood (UCB) and tissue (UCT) into skeletal muscle have remained underexplored. Here, we present a comparative analysis of UCB and UCT MSCs, in terms of surface markers, proliferation and senescence marker expression. We find that CD45−CD34− MSCs obtained from UCT and UCB of term births display differences in the combinatorial expression of key MSC markers CD105 and CD90. Importantly, UCT MSCs display greater yield, higher purity, shorter culture time, and lower rates of senescence in culture compared to UCB MSCs. Using a robust myogenic differentiation protocol, we show that UCT MSCs differentiate more robustly into muscle than UCB MSCs by transcriptomic sequencing and specific myogenic markers. Functional assays reveal that CD90, and not CD105 expression promotes myogenic differentiation in MSCs and could explain the enhanced myogenic potential of UCT MSCs. These results suggest that in comparison to large volumes of UCB that are routinely used to obtain MSCs and with limited success, UCT is a more reliable, robust, and convenient source of MSCs to derive cells of the myogenic lineage for both therapeutic purposes and increasing our understanding of developmental processes.

scholarly journals Mesenchymal stem cells isolated from peripheral blood and umbilical cord Wharton’s jelly

2013 ◽  
Vol 141 (3-4) ◽  
pp. 178-186 ◽  
Author(s):  
Drenka Trivanovic ◽  
Jelena Kocic ◽  
Slavko Mojsilovic ◽  
Aleksandra Krstic ◽  
Vesna Ilic ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Peripheral Blood ◽  
Myogenic Differentiation ◽  
Smooth Muscle Actin ◽  
Culture Method ◽  
Good Alternative ◽  
Explant Culture ◽  
Differentiation Potential

Introduction. Mesenchymal stem cells (MSCs) are a promising tool for regenerative medicine, but due to the heterogeneity of their populations, different sources and isolation techniques, the characteristics defining MSCs are inconsistent. Objective. The aim of this study was to compare the characteristics of MSCs derived from two different human tissues: peripheral blood (PB-MSCs) and umbilical cord Wharton?s Jelly (UC-MSCs). Methods. The PB-MSC and UC-MSC were isolated by adherence to plastic after gradient-density separation or an explant culture method, respectively, and compared regarding their morphology, clonogenic efficiency, proliferating rates, immunophenotype and differentiation potential. Results. MSCs derived from both sources exhibit similar morphology, proliferation capacity and multilineage (osteogenic, chondrogenic, adipogenic and myogenic) differentiation potential. Differences were observed in the clonogenic capacity and the immunophenotype, since UC-MSCs showed higher CFU-F (colony-forming units-fibroblastic) cloning efficiency, as well as higher embryonic markers (Nanog, Sox2, SSEA4) expression. When additional surface antigens were analyzed by flow cytometry (CD44, CD90, CD105, CD33, CD34, CD45, CD11b, CD235a) or immunofluorescent labeling (vimentin, STRO-1 and ?-smooth muscle actin), most appeared to have similar epitope profiles irrespective of MSC source. Conclusion. The results obtained demonstrated that both MSCs represent good alternative sources of adult MSCs that could be used in cell therapy applications.

scholarly journals The effect of extracellular matrix on myogenic induction of adipose mesenchymal stem cells

2020 ◽  
Author(s):  
Wenyong Fei ◽  
bin xie ◽  
Ying Liu ◽  
Mingsheng Liu ◽  
Xuanqi Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Myogenic Differentiation ◽  
Subcutaneous Fat ◽  
Myoblast Differentiation ◽  
Differentiation Potential ◽  
Culture Bottle ◽  
Significant Difference ◽  
Adipose Mesenchymal Stem Cells ◽  
Myogenic Induction

Abstract Bankground: As one of the hot cells in the field of regenerative medicine, adipose mesenchymal stem cells(ADSCs) have been proved to have the ability of myoblast differentiation, but the disadvantage is that the efficiency of myoblast differentiation is not very high.Extracellular matrix(ECM), as a mixture of cytokines and proteins secreted by cells, is the substance secreted by cells. It has the advantages of non-toxic and harmless, and can provide the necessary material and environmental basis for cell growth and development.This study was to explore whether the myogenic differentiation potential of ADSCs cultured in ECM was improved.Method: ADSCs were extracted from subcutaneous fat of male SD rats at the age of 3 months and weight 250g. The third generation cellular of ADSCs were prepared for ECM，and the ECM was contained in the culture bottle for amplify the ADSCs. Myogenic induction was performed by 5-azacytidine using the same generation of ADSCs cultured with ECM and non-ECM.Result: ECM can significantly increase the rate of cell proliferation (P<0.05).Immunofluorescence detected the expression of β-actin, Myod and Desmin in cells ,and showed no significant difference in the expression of β-actin between groups (P>0.05). However, there were significant differences in Myod and Desmin between groups (P<0.001).RT-PCR showed that the mRNA expressions of myosin heavy chain(MHC), troponin, Myogein and Myf5 were significantly different among groups (P<0.001).Conclusion: ECM culture of ADSCs can improve its growth rate and myogenic differentiation potential.

scholarly journals Mesenchymal stem cells isolated from human periodontal ligament

2014 ◽  
Vol 66 (1) ◽  
pp. 261-271 ◽  
Author(s):  
Maja Miletic ◽  
S. Mojsilovic ◽  
Ivana Okic-Djordjevic ◽  
Tamara Kukolj ◽  
Aleksandra Jaukovic ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Periodontal Ligament ◽  
Myogenic Differentiation ◽  
Mesenchymal Cell ◽  
Stem Cell Markers ◽  
Differentiation Potential ◽  
Hematopoietic Stem ◽  
Cell Markers ◽  
Multipotent Cells

Mesenchymal stem cells (MSCs) were isolated from human periodontal ligament (hPDL-MSCs) and characterized by their morphology, clonogenic efficiency, proliferation and differentiation capabilities. hPDL-MSCs, derived from normal impacted third molars, possessed all of the properties of MSC, including clonogenic ability, high proliferation rate and multi-lineage (osteogenic, chondrogenic, adipogenic, myogenic) differentiation potential. Moreover, hPDL-MSCs expressed a typical MSC epitope profile, being positive for mesenchymal cell markers (CD44H, CD90, CD105, CD73, CD29, Stro-1, fibronectin, vimentin, ?-SMA), and negative for hematopoietic stem cell markers (CD34, CD11b, CD45, Glycophorin-CD235a). Additionally, hPDL-MSCs, as primitive and highly multipotent cells, showed high expression of embryonic markers (Nanog, Sox2, SSEA4). The data obtained provided yet further proof that cells with mesenchymal properties can be obtained from periodontal ligament tissue. Although these cells should be further investigated to determine their clinical significance, hPDL-MSCs are believed to provide a renewable and promising cell source for new therapeutic strategies in the treatment of periodontal defects.

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