Behavioral appraisal by implementing a short sequence of stress resolves adaptively changed stress gains

Abstract Chronic stress produces adaptive changes in the brain via the cumulative action of glucocorticoids, which causes psychiatric illnesses such as depression. Here we show that a behavioral method implementing weak stress does not strengthen but resolves existing stress gains. Chronic stress produces persistent depressive behaviors in mice, and repeated daily treatment with 5-min restraint produces antidepressive effects. Repeated treatment with low-dose glucocorticoids mimics the anti-depressive effects of weak stress. Repeated weak stress or low-dose glucocorticoid treatment distinctively activates the prelimbic cortex (PL), and reverses the stress-induced altered gene expression profiles. Chemogenetic inhibition of PL outputs projecting to the nucleus accumbens, basolateral amygdala, or bed nucleus of the stria terminalis (BNST) dissipates antidepressive effects of weak stress, but only the PL-to-BNST circuit produces changes in dysregulated glucocorticoid release. Our results suggest that behavioral appraisal by implementing weak stress can resolve adaptively altered stress gains and rectify stress-induced depressive behaviors.

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Repeated exposure with short-term behavioral stress resolves pre-existing stress-induced depressive-like behavior in mice

Nature Communications ◽

10.1038/s41467-021-26968-4 ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Eun-Hwa Lee ◽

Jin-Young Park ◽

Hye-Jin Kwon ◽

Pyung-Lim Han

Keyword(s):

Basolateral Amygdala ◽

Expression Profiles ◽

Gene Expression Profiles ◽

Repeated Exposure ◽

Repeated Injection ◽

Stria Terminalis ◽

Prelimbic Cortex ◽

Short Term ◽

Bed Nucleus ◽

The Brain

AbstractChronic stress induces adaptive changes in the brain via the cumulative action of glucocorticoids, which is associated with mood disorders. Here we show that repeated daily five-minute restraint resolves pre-existing stress-induced depressive-like behavior in mice. Repeated injection of glucocorticoids in low doses mimics the anti-depressive effects of short-term stress. Repeated exposure to short-term stress and injection of glucocorticoids activate neurons in largely overlapping regions of the brain, as shown by c-Fos staining, and reverse distinct stress-induced gene expression profiles. Chemogenetic inhibition of neurons in the prelimbic cortex projecting to the nucleus accumbens, basolateral amygdala, or bed nucleus of the stria terminalis results in anti-depressive effects similarly to short-term stress exposure, while only inhibition of neurons in the prelimbic cortex projecting to the bed nucleus of the stria terminalis rescues defective glucocorticoid release. In summary, we show that short-term stress can reverse adaptively altered stress gains and resolve stress-induced depressive-like behavior.

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PSIV-11 Effects of very low-dose antibiotics on gene expression profiles in ileal mucosa of weaned pigs infected with a pathogenic E. coli

Journal of Animal Science ◽

10.1093/jas/skaa278.515 ◽

2020 ◽

Vol 98 (Supplement_4) ◽

pp. 286-286

Author(s):

Kwangwook Kim ◽

Sungbong Jang ◽

Yanhong Liu

Keyword(s):

Gene Expression ◽

Systemic Inflammation ◽

Low Dose ◽

Expression Profiles ◽

Gene Expression Profiles ◽

Post Inoculation ◽

Growth Promoter ◽

Weaned Pigs ◽

Ileal Mucosa ◽

E Coli

Abstract Our previous studies have shown that supplementation of low-dose antibiotic growth promoter (AGP) exacerbated growth performance and systemic inflammation of weaned pigs infected with pathogenic Escherichia coli (E. coli). The objective of this experiment, which is extension of our previous report, was to investigate the effect of low-dose AGP on gene expression in ileal mucosa of weaned pigs experimentally infected with F18 E. coli. Thirty-four pigs (6.88 ± 1.03 kg BW) were individually housed in disease containment rooms and randomly allotted to one of three treatments (9 to 13 pigs/treatment). The three dietary treatments were control diet (control), and 2 additional diets supplemented with 0.5 or 50 mg/kg of AGP (carbadox), respectively. The experiment lasted 18 d [7 d before and 11 d after first inoculation (d 0)]. The F18 E. coli inoculum was orally provided to all pigs with the dose of 1010 cfu/3 mL for 3 consecutive days. Total RNA [4 to 6 pigs/treatment on d 5; 5 to 7 pigs/treatment on 11 post-inoculation (PI)] was extracted from ileal mucosa to analyze gene expression profiles by Batch-Tag-Seq. The modulated differential gene expression were defined by 1.5-fold difference and a cutoff of P < 0.05 using limma-voom package. All processed data were statistically analyzed and evaluated by PANTHER classification system to determine the biological process function of genes in these lists. Compared to control, supplementation of recommended-dose AGP down-regulated genes related to inflammatory responses on d 5 and 11 PI; whereas, feeding low-dose AGP up-regulated genes associated with negative regulation of metabolic process on d 5, but down-regulated the genes related to immune responses on d 11 PI. The present observations support adverse effects of low-dose AGP in our previous study, indicated by exacerbated the detrimental effects of E. coli infection on pigs’ growth rate, diarrhea and systemic inflammation.

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Effect of low-dose hydrocortisone on gene expression profiles after severe burn injury

Critical Care ◽

10.1186/cc13265 ◽

2014 ◽

Vol 18 (Suppl 1) ◽

pp. P75

Author(s):

J Plassais ◽

MA Cazalis ◽

F Venet ◽

G Monneret ◽

A Pachot ◽

...

Keyword(s):

Gene Expression ◽

Low Dose ◽

Burn Injury ◽

Expression Profiles ◽

Gene Expression Profiles ◽

Severe Burn ◽

Severe Burn Injury

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Integrative Genomics with Mediation Analysis in a Survival Context

Computational and Mathematical Methods in Medicine ◽

10.1155/2013/413783 ◽

2013 ◽

Vol 2013 ◽

pp. 1-8 ◽

Cited By ~ 2

Author(s):

Szilárd Nemes ◽

Toshima Z. Parris ◽

Anna Danielsson ◽

Zakaria Einbeigi ◽

Gunnar Steineck ◽

...

Keyword(s):

Expression Profiles ◽

Gene Expression Profiles ◽

Genomic Analysis ◽

Mrna Levels ◽

Integrative Genomics ◽

Asymptotic Results ◽

Data Set ◽

Cancer Data ◽

Dna And Rna ◽

Altered Gene

DNA copy number aberrations (DCNA) and subsequent altered gene expression profiles may have a major impact on tumor initiation, on development, and eventually on recurrence and cancer-specific mortality. However, most methods employed in integrative genomic analysis of the two biological levels, DNA and RNA, do not consider survival time. In the present note, we propose the adoption of a survival analysis-based framework for the integrative analysis of DCNA and mRNA levels to reveal their implication on patient clinical outcome with the prerequisite that the effect of DCNA on survival is mediated by mRNA levels. The specific aim of the paper is to offer a feasible framework to test the DCNA-mRNA-survival pathway. We provide statistical inference algorithms for mediation based on asymptotic results. Furthermore, we illustrate the applicability of the method in an integrative genomic analysis setting by using a breast cancer data set consisting of 141 invasive breast tumors. In addition, we provide implementation in R.

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