Spectrophotometric Method for the Determination of Metronidazole in Pharmaceutical products based on the Formation of Azo dye

A spectrophotometric method has been developed for the determination of [(2-chlorophenyl)methylene]propane dinitrile (CS) stabilized by the diazonium salts Fast Red TR and Fast Blue B after extraction with chloroform. Mass spectrometry, 1H and 13C NMR and elemental analysis confirmed the presence of the hydrazoform of the azo dye formed by reaction with malononitrile, as a product of the alkaline hydrolysis of substance CS, and the diazonium salt Fast Red TR.

Download Full-text

Design and optimization of a chemometrics-assisted spectrophotometric method for the simultaneous determination of levodopa and carbidopa in pharmaceutical products

Analytica Chimica Acta ◽

10.1016/j.aca.2005.04.065 ◽

2005 ◽

Vol 543 (1-2) ◽

pp. 192-198 ◽

Cited By ~ 24

Author(s):

Patricia C. Damiani ◽

Andrea C. Moschetti ◽

Adrián J. Rovetto ◽

Fernando Benavente ◽

Alejandro C. Olivieri

Keyword(s):

Simultaneous Determination ◽

Spectrophotometric Method ◽

Pharmaceutical Products ◽

Design And Optimization

Download Full-text

Spectrophotometric determination of methyldopa in pharmaceutical formulations

Eclética Química ◽

10.1590/s0100-46702005000300003 ◽

2005 ◽

Vol 30 (3) ◽

pp. 23-28 ◽

Cited By ~ 7

Author(s):

P. R. S. Ribeiro ◽

L. Pezza ◽

H. R. Pezza

Keyword(s):

Correlation Coefficient ◽

Spectrophotometric Method ◽

Confidence Level ◽

Low Cost ◽

Pharmaceutical Preparations ◽

Pharmaceutical Formulations ◽

Pharmaceutical Products ◽

Complexation Reaction ◽

Excellent Correlation

A new, simple, precise, rapid and low-cost spectrophotometric method for methyldopa determination in pharmaceutical preparations is described. This method is based on the complexation reaction of methyldopa with molybdate. Absorbance of the resulting yellow coloured product is measured at 410 nm. Beer's Law is obeyed in a concentration range of 50 - 200 µg ml-1 methyldopa with an excellent correlation coefficient (r = 0.9999). No interference was observed from common excipients in formulations. The results show a simple, accurate, fast and readily applied method to the determination of methyldopa in pharmaceutical products. The analytical results obtained for these products by the proposed method are in agreement with those of the Brazilian Pharmacopoeia procedure at 95% confidence level.

Download Full-text

A Simple, Sensitive Spectrophotometric Determination of Mosapride in Pharmaceutical Preparations Using Novel Reagent

E-Journal of Chemistry ◽

10.1155/2004/285604 ◽

2004 ◽

Vol 1 (5) ◽

pp. 267-271 ◽

Cited By ~ 1

Author(s):

G. Krishnamurthy Naidu ◽

K. Suvardhan ◽

M. V. Jyothi ◽

B. S. Sastry ◽

P. Chiranjeevi

Keyword(s):

Optimum Condition ◽

Spectrophotometric Determination ◽

Spectrophotometric Method ◽

Azo Dye ◽

Acidic Medium ◽

Pharmaceutical Preparations ◽

Coupling Reaction ◽

Pharmaceutical Formulation ◽

Sensitive Spectrophotometric Method

A rapid, selective and sensitive spectrophotometric method for the determination of mosarpide in either pure form or in its formulations in pharmaceutical preparations is described. The method is based on the diazotization coupling reaction of mosapride with 1-naphthol in acidic medium to give red azo dye product with theλmaxof 500 nm and is stable for more than 12 hours at optimum condition, Beer's law is obeyed at concentration of 0.2 to 12 μg mL-1at the wavelength of maximum absorbance. Common excipients used as additives in pharmaceutical preparations do not interfere in the proposed method. The developed method is highly reproducible and applied to wide variety of pharmaceutical formulation and the results were compared favourably with the reported method.

Download Full-text

DETERMINATION OF THIAMINE HYDROCHLORIDE AND PYRIDOXINE HYDROCHLORIDE ON RICE WITH WASHING, MILLING AND POUNDING BY SPECTROMETRIC METHODS

Journal of Islamic Pharmacy ◽

10.18860/jip.v3i2.6136 ◽

2018 ◽

Vol 3 (2) ◽

pp. 30

Author(s):

Edy Agustian Yazid ◽

Damar Pasunaring Ati ◽

Aulia Mahardika

Keyword(s):

Alkaline Solution ◽

Spectrophotometric Method ◽

Azo Dye ◽

Food Source ◽

Coupling Reaction ◽

Statistical Test ◽

Thiamine Hydrochloride ◽

Pyridoxine Hydrochloride ◽

Significant Difference

<em>Rice is a principal food source for most of the world's population. Rice has many nutrients including thiamin hydrocloride (THC) and pyrodoxine hydrocloride (PHC). Before consumption, rice is usually processed such as milling, washing, and cooking. Determination of THC on IR-64 and Rojolele rice with washing three times has been conducted. Also determined of PHC on the milled IR-64 rice and the pounded IR-64 rice. The THC was determined by spectrophotometric method at 246 nm. Determination of PHC based on the coupling reaction with diazo reagent in alkaline solution to form azo dye then the absorption was measured at 440 nm. The result of determination of THC on IR-64 and Rojolele rice after washing three times can decrease THC level 12,93% and 12,88% respectively. Determination of PHC on the milled IR-64 rice obtained 1.54 ± 0.027 mg/g and on the pounded IR-64 rice 1.80 ± 0.048 mg/g. The result of statistical test with independent T test showed there were significant difference (α = 0,05) PHC level on both treatments.</em>

Download Full-text

Determination of Fluoroquinolones in Pharmaceutical Formulations by Extractive Spectrophotometric Methods Using Ion-Pair Complex Formation with Bromothymol Blue

Journal of Analytical Methods in Chemistry ◽

10.1155/2018/8436948 ◽

2018 ◽

Vol 2018 ◽

pp. 1-11 ◽

Cited By ~ 1

Author(s):

Trung Dung Nguyen ◽

Hoc Bau Le ◽

Thi Oanh Dong ◽

Tien Duc Pham

Keyword(s):

Spectrophotometric Method ◽

High Performance ◽

Limit Of Detection ◽

Pharmaceutical Formulations ◽

Bromothymol Blue ◽

Pharmaceutical Products ◽

Ion Pair ◽

Spectrophotometric Methods ◽

Method Of Continuous Variation

In this paper, we reported a new, simple, accurate, and precise extractive spectrophotometric method for the determination of fluoroquinolones (FQs) including ciprofloxacin (CFX), levofloxacin (LFX), and ofloxacin (OFX) in pharmaceutical formulations. The proposed method is based on the ion-pair formation complexes between FQs and an anionic dye, bromothymol blue (BTB), in acidic medium. The yellow-colored complexes which were extracted into chloroform were measured at the wavelengths of 420, 415, and 418 nm for CFX, LFX, and OFX, respectively. Some effective conditions such as pH, dye concentration, shaking time, and organic solvents were also systematically studied. Very good limit of detection (LOD) of 0.084 µg/mL, 0.101 µg/mL, and 0.105 µg/mL were found for CFX, LFX, and OFX, respectively. The stoichiometry of the complexes formed between FQs and BTB determined by Job’s method of continuous variation was 1 : 1. No interference was observed from common excipients occurred in pharmaceutical formulations. The proposed method has been successfully applied to determine the FQs in some pharmaceutical products. A good agreement between extractive spectrophotometric method with high-performance liquid chromatography mass spectrometry (HPLC-MS) for the determination of FQs in some real samples demonstrates that the proposed method is suitable to quantify FQs in pharmaceutical formulations.

Download Full-text