scholarly journals THE EFFECTS OF THE INTERACTION BETWEEN LIGHT AND EXOGENOUS ABSCISIC ACID ON COLD ACCLIMATION OF `ALASKA' PEA SEEDLINGS.

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 685b-685
Author(s):  
Milton E. Tignor ◽  
Russell L. Weiser
Keyword(s):  
Abscisic Acid ◽  
Pisum Sativum ◽  
Plant Growth ◽  
Cold Acclimation ◽  
Combination Treatment ◽  
Distilled Water ◽  
Pea Seedlings ◽  
Cold Tolerant

Alaska peas (Pisum sativum `Alaska') were germinated in the dark at 25C. After three days, when the shoots were approx. 1.5 cm, treatments were initiated. ABA, at 10-4M, was exogenously applied through the root solution. The control peas remained in distilled water. All treatments involving the application of ABA were applied under green safe light. Light treatments were applied using overhead fluorescent lights for designated timed intervals (0 to 20 min) over 3 days. A methanol bath was then used to induce freezing stresses from 0 to -9C. The combination treatment of light and ABA had the lowest LT50 (more cold tolerant) followed by light, dark, and dark with ABA (least cold tolerant). Extensin levels, plant growth, and stem bendability were also recorded.

scholarly journals RESPONSE OF `ALASKA' PEA SEEDLINGS TO FREEZING AND CHILLING STRESSES AFTER EXOGENOUS APPLICATION OF ABSCISIC ACID.

HortScience ◽  
1991 ◽  
Vol 26 (6) ◽  
pp. 737B-737
Author(s):  
Milton E. Tignor ◽  
Russell L. Weiser
Keyword(s):  
Amino Acids ◽  
Cell Wall ◽  
Abscisic Acid ◽  
Chilling Stress ◽  
Dry Weight ◽  
Visual Observation ◽  
Distilled Water ◽  
Fresh Weight ◽  
Pea Seedlings ◽  
Blank Solution

Alaska peas (Pisum sativum `Alaska') germinated in a dark growth chamber were treated ABA dissolved in a small amount of acetone before diluting in distilled water with 0.1% spreader. A blank solution was identically prepared without ABA. Both solutions were applied via paintbrush to the epicotyls of the peas every twelve hours for seven days following emergence. The blank solution was applied to two controls, chronological and physiological. A methanol bath was used to induce freezing and chilling stresses. ABA significantly improved cold tolerance (electrolyte leakage) in the pea seedlings for both freezing and chilling stress as compared to the physiological and chronological controls. Visual observation of the pea stems suggested a difference in stem flexibility among ABA treated peas and the controls. Pea stem elasticity and plasticity were measured along with plant dry weight, cell wall weight/gram fresh weight, and the quantity of cell wall sugars and amino acids.

scholarly journals The effect of fluridone and flurochloridone on the incidence of albinism in pea (Pisum sativum) and on the abscission of leaves of privet (Ligustrum vulgare)

2011 ◽  
Vol 48 (No. 6) ◽  
pp. 255-260
Author(s):  
Š. Klíčová ◽  
J. Šebánek ◽  
M. Hudeová ◽  
H. Vítková ◽  
H. Vlašínová
Keyword(s):  
Abscisic Acid ◽  
Pisum Sativum ◽  
Leaf Blade ◽  
Acid Synthesis ◽  
Apical Part ◽  
Concentrated Solutions ◽  
Pea Seedlings ◽  
Ligustrum Vulgare ◽  
High Concentrations ◽  
Cut Surface

The effect of fluridone (or flurochloridone), the inhibitor of carotenoid, chlorophyll and abscisic acid synthesis, on the abscission of Ligustrum vulgare leaves was investigated. Both forms of fluridone inhibited the abscission of petioles when they were applied as a 1.0% concentration in lanolin to the leaf blade. Fluridone was capable of inhibiting abscission even when it was applied to the petiole as late as 7 hours after the blade was cut off. Fluridone applied in lanolin to the apical part of intact pea seedlings or on the cut surface after decapitation of the epicotyl caused albinism of the stipules. The degree of albinism decreased according to the concentration of applied flurodine (from the highest – 0.5% to the lowest – 0.03%) and was higher in intact than in decapitated plants. Albinism also appeared in pea seedlings grown from seeds swollen in differently concentrated solutions of flurochloridone, particularly in the basal part of the shoots. Complete albinism occurred only in plants cultivated from seeds swollen in high concentrations (0.06–0.12%). The lowest concentration (0.007%) stimulated root growth of the pea seedlings, but inhibited epicotyl growth.

scholarly journals Tracking of Zinc Ferrite Nanoparticle Effects on Pea (Pisum sativum L.) Plant Growth, Pigments, Mineral Content and Arbuscular Mycorrhizal Colonization

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 583
Author(s):  
Reda E. Abdelhameed ◽  
Nagwa I. Abu-Elsaad ◽  
Arafat Abdel Hamed Abdel Latef ◽  
Rabab A. Metwally
Keyword(s):  
Pisum Sativum ◽  
Plant Growth ◽  
Zinc Ferrite ◽  
Crop Improvement ◽  
Nanocrystalline Structure ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Transmission Electron ◽  
Agricultural Applications ◽  
The Impact

Important gaps in knowledge remain regarding the potential of nanoparticles (NPs) for plants, particularly the existence of helpful microorganisms, for instance, arbuscular mycorrhizal (AM) fungi present in the soil. Hence, more profound studies are required to distinguish the impact of NPs on plant growth inoculated with AM fungi and their role in NP uptake to develop smart nanotechnology implementations in crop improvement. Zinc ferrite (ZnFe2O4) NPs are prepared via the citrate technique and defined by X-ray diffraction (XRD) as well as transmission electron microscopy for several physical properties. The analysis of the XRD pattern confirmed the creation of a nanocrystalline structure with a crystallite size equal to 25.4 nm. The effects of ZnFe2O4 NP on AM fungi, growth and pigment content as well as nutrient uptake of pea (Pisum sativum) plants were assessed. ZnFe2O4 NP application caused a slight decrease in root colonization. However, its application showed an augmentation of 74.36% and 91.89% in AM pea plant shoots and roots’ fresh weights, respectively, compared to the control. Moreover, the synthesized ZnFe2O4 NP uptake by plant roots and their contents were enhanced by AM fungi. These findings suggest the safe use of ZnFe2O4 NPs in nano-agricultural applications for plant development with AM fungi.

scholarly journals Method for maintenance of coffee leaves in vitro for mass rearing of Leucoptera coffeellum (Guérin-Méneville) (Lepidoptera: Lyonetiidae)

2000 ◽  
Vol 29 (4) ◽  
pp. 849-854 ◽  
Author(s):  
Ronaldo Reis Jr. ◽  
Lima ◽  
Evaldo F. Vilela ◽  
Raimundo S. Barros
Keyword(s):  
Plant Growth ◽  
Mass Rearing ◽  
Developmental Cycle ◽  
Plant Tissues ◽  
Distilled Water ◽  
Complete Development ◽  
Detached Leaves ◽  
Leaf Survival ◽  
The Ideal

To accomplish systematic studies with coffee leafminer, it is necessary to establish a mass rearing system under artificial conditions. It is possible to rear this species, from egg to adult, under laboratory conditions, without using coffee seedlings but detached leaves maintained in vitro. Synthetic cytokinins are routinely used for maintenance of plant cell and plant tissues in vitro. Two plant growth regulators, benzyladenin and kinetin, in concentrations 10-6 and 10-7 M were used to mantain the leaves. Green leaves collected in the field were maintained in the solution to be tested. Distilled water served as control. The experiment lasted 30 days, a period longer than the necessary for the complete development of the insect. Both artificial cytokinines indeed increased the lifetime of the coffee leaves, maintaining them green and healthy. Leaves placed in the cages for oviposition were attractive to the insect, with significant number of eggs per leaf. In most cases, eggs resulted in individuals that completed the whole developmental cycle. Tests with regulator in different concentrations with healthy leaves showed efficiency. However, we believe that hormone concentrations to be used with mined leaves should be larger, because these when maintained at 10-7 M leaves did not present a satisfactory lifetime. Therefore, tests with mined leaves with different hormone concentrations should be made to find out the ideal concentration for leaf survival. In our laboratory we are successfully using 10-6 M benzyladenin for the maintenance of mined leaves.

scholarly journals AtMybL-O modulates abscisic acid biosynthesis to optimize plant growth and ABA signaling in response to drought stress

2018 ◽  
Vol 61 (4) ◽  
pp. 473-477 ◽  
Author(s):  
Chan Young Jeong ◽  
Won Je Lee ◽  
Hai An Truong ◽  
Cao Sơn Trịnh ◽  
Suk-Whan Hong ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Drought Stress ◽  
Plant Growth ◽  
Aba Signaling ◽  
Acid Biosynthesis ◽  
Abscisic Acid Biosynthesis

scholarly journals Longevity of wild pansy flowers treated with growth regulators

2018 ◽  
Vol 24 (2) ◽  
pp. 103-108
Author(s):  
Tania Pires Da Silva ◽  
Fernanda Ferreira Araujo ◽  
Fernando Luiz Finger
Keyword(s):  
Weight Loss ◽  
Abscisic Acid ◽  
Growth Regulators ◽  
Water Status ◽  
Fluorescent Light ◽  
Distilled Water ◽  
Fresh Weight ◽  
Exogenous Ethylene ◽  
Fourth Experiment ◽  
And Control

The objective of this study was to evaluate the growth regulators action on the senescence of wild pansy flowers. In the first experiment, floral stems were treated with ethylene for 24 hours at concentrations of 0.1, 1.0, 10, 100 and 1000 μL L-1 and control without the hormone. In a second experiment, the flowers were immersed in solutions of abscisic acid (ABA) containing 5, 20, 50 and 100 μM for one minute and control with water. In a third experiment, 1-methylcyclopropene (1-MCP) was applied at concentrations of 0.5, 1.0 and 1.5 μL L-1 and control without the chemical. In a fourth experiment, 1-MCP and ethylene were applied, where 1-MCP was first applied followed by ethylene. After the treatments with 1-MCP and ethylene, the floral stems were removed from the hermetic chambers and kept in a vessel containing distilled water at 25 °C, 10 μmol m-2 s- 1 white fluorescent light and 50-70% relative humidity as for the ABA treatment. Flowers treated with ethylene did not present significant differences among the concentrations for visual senescence, showing evidence that this flower is not sensitive to ethylene. Treatment with 1000 μL L-1 of ethylene led to a slightly higher fresh weight loss than other treatments, which had a loss of about 33% at end of the experiment. For the ABA treatment, the flowers showed similar fresh weight loss among the different treatments; however, higher concentrations induced slight senescence of flowers. The use of 1-MCP increased the longevity of wild pansy flowers. These results show that 1-MCP is beneficial in maintaining the flower water status, even in the presence of exogenous ethylene, although ethylene may not be directly involved in the senescence of wild pansy flowers.

scholarly journals Abscisic acid as a factor in regulation of photosynthetic carbon metabolism of pea seedlings

2014 ◽  
Vol 51 (2) ◽  
pp. 229-240 ◽  
Author(s):  
Maria Faltynowicz ◽  
Waldemar Lechowicz ◽  
Jerzy Poskuta
Keyword(s):  
Abscisic Acid ◽  
Carbon Metabolism ◽  
Rubp Carboxylase ◽  
Pep Carboxylase ◽  
Pea Seedlings ◽  
Photosynthetic Carbon ◽  
Photosynthetic Carbon Metabolism ◽  
Sugar Phosphates ◽  
Reduced Activity ◽  
Endogenous Aba

The influence of abscisic acid (ABA) on carbon metabolism and the activity of ribulosebisphosphate (RuBP) and phosphoenolpyruvate (PEP) carboxylases in 8-day-old pea seedlings was investigated. It was endeavoured to correlate the changes observed in metabolic processes with the endogenous ABA level. In plants treated with ABA incorporation of labeled carbon into sucrose, glucose, fructose and sugar phosphates was depressed, while <sup>14</sup>C incorporation into starch, ribulose and malic acid was enhanced. The activity of RuBP carboxylase was considerably lowered, whereas that of PEP carboxylase was slightly increased. It is considered that inhibition of photosynthesis due to the action of ABA is caused to a great extent by the obstruction of the C-3 pathway and reduced activity of RuBP carboxylase, whereas (β-carboxylation was not blocked.

Sign in / Sign up

Export Citation Format

Share Document