757 PB 155 LABORATORY RESPONSE TO DORMANCY-BREAKING TREATMENTS DIFFERS BY BUD AND CUTTING TYPE IN PEACH
Endodormant `Hawthorne' peach shoots were collected in early autumn and sectioned into long (30-40 cm), short terminal (10-15 cm), or short sub-terminal (IO-15 cm) cuttings. Dormancy-breaking treatments included application of hydrogen cyanamide H2C N2), thiourea (TU), sodium azide (NaN3) or gibberellic acid (GA3) solutions; atmospheric methyl jasmonate (MJ); hot water (50C for 1-2 h); or chilling (3.5C for 1-4 weeks). During forcing at 24C, & budbreak of all bud types on long cuttings was very low. On short cuttings, % apical budbreak was greater than % lateral vegetative budbreak, with almost no floral budbreak. Relative to H2CN2-induced lateral vegetative budbreak, budbreak induced by MJ, TIJ, GA,, and NaN3 was 17, 34, 50, and 92%, respectively. Relative apical budbreak was 0, 95, 53, and 63%, respectively. Addition of aminooxyacetic acid (AOA) to the beaker solution (in which cuttings were forced) induced apical, but not lateral, budbreak by itself; AOA synergistically improved H2CN2-induced budbreak by 23%. Latetal budbreak on short sub-terminal cuttings treated with hot water (1 h) was similar to that of H2CN2 treatment. Chilling increased apical budbreak to 100% as duration increased to 3 weeks, lateral vegetative budbreak only reached 43% after 4 weeks of treatment. The use of different bud and cutting types as model systems to study the differential regulation of dormancy by various treatments will be discussed.