scholarly journals Freezing Tolerance and Cold Acclimation in Guava (Psidium guajava L.)

HortScience ◽  
2009 ◽  
Vol 44 (5) ◽  
pp. 1258-1266 ◽  
Author(s):  
Wei Hao ◽  
Rajeev Arora ◽  
Anand K. Yadav ◽  
Nirmal Joshee

Guava (Psidium guajava L.) is a tropical evergreen tree that tolerates a wide range of frost-free environments. In recent years, the American market demand for exotic and nutritious fruits, like guava, has been increasing, and, with a long harvest period, guava can be a potential alternative, high-value cash crop in the United States. However, the major limitation with commercializing guava cultivation in the United States is its low cold tolerance. In this article, we studied the physiology of freezing tolerance and cold acclimation in guava. Laboratory freeze–thaw tests (on leaves), shoot growth and leaf relative water content measurements, leaf anthocyanin content analyses, and leaf protein analyses were performed on nonacclimated and cold-acclimated guava cultivars Lucknow-49 and Ruby × Supreme. The leaf freezing tolerance (expressed as LT50 values) of nonacclimated tissues was ≈–2.5 °C and significantly enhanced to ≈–4.4 °C after an environmentally controlled cold acclimation regime for both cultivars. However, when compared based on actual injury sustained by leaves at various freezing temperatures in a freeze–thaw test, ‘Ruby × Supreme’ exhibited significantly less injury than ‘Lucknow-49’ at most temperatures. Growth and leaf relative water content reduced, whereas leaf anthocyanins accumulated during cold acclimation. Leaf protein analyses, which were performed after cold acclimation and drought stress, revealed that four proteins (69, 48, 23.5, and 17.4 kDa) accumulated in response to low temperatures, and two proteins (17.4 and 16 kDa) accumulated in response to drought stress. Antidehydrin immunoblots revealed that one common 17.4 kDa dehydrin accumulated in response to cold and drought stresses. Our data indicate that guava possesses leaf freezing tolerance, exhibits cold acclimation ability, and that ‘Ruby × Supreme’ leaves are relatively more freezing-tolerant than ‘Lucknow-49’ when compared up to –4 and –8 °C for nonacclimated and cold-acclimated tissues, respectively. Cold acclimation in guava appears to be a multifactorial process involving complex physiological and biochemical changes and also overlapping responses with drought stress.

Author(s):  
Agustina Asri Rahmianna ◽  
Dan Joko Purnomo

Drought stress during generative stage affected pod yield, yield components, seed and pod qualities of groundnut (Arachis hypogaea L.). The reseach was carried out to assess the effect of drought stress at various soil water availabilities during generative stage on pod yield, pod and seed physical qualities. The experiment was conducted at Muneng Experimental Farm, Probolinggo District during July-October 2012. Five genotypes were arranged in a RCB design, with 3 replicates. The replications were nested into four treatments of soil water availability (0-100, 0-85, 0-70,0-55 days after sowing/DAS). The pods were harvested at 102 days after sowing. The result showed that the shorter the water availability, the lower the leaf relative water content, pod and seed water contents, number of mature pods, seed size, and intact seeds weight. Pod yield reduced when water was available upto 55 DAS only. Turangga variety had the highest pod yield (1.626 ton ha-1) with low pod and seed physical qualities. GH-51 yielded in 1.076 ton ha-1 with superior pod and seed physical qualities. Despite of its lowest pod yield (0.964 ton ha-1), J-11 produced the same pod and seed physical qualities as GH 51 did. ICGV 86590 was superior on its pod yield (1.338 ton ha-1) with low pod and seed physical qualities. Kancil variety did not perform any superiority.<br /><br />Keywords: intact seeds, leaf relative water content, pod moisture content, seed moisture content


HortScience ◽  
2013 ◽  
Vol 48 (10) ◽  
pp. 1309-1312 ◽  
Author(s):  
Smita Barkataky ◽  
Robert C. Ebel ◽  
Kelly T. Morgan ◽  
Keri Dansereau

This study was conducted on well-watered citrus to determine changes in water relations during cold acclimation independent of drought stress. Potted sweet orange and Satsuma mandarin trees were exposed to progressively lower, non-freezing temperatures down to 10/4 °C, light/dark temperatures, respectively, for 9 weeks in environmental growth chambers to promote cold acclimation. The trees were watered twice daily and three times on the day water relations data were collected to minimize drought stress. Although soil moisture was higher and non-limiting for plants in the cold than in the warm chamber, cold temperatures promoted stomatal closure, higher root resistance, lower stem water potential (Ψstem), lower transpiration, and lower leaf ψS. Leaf relative water content (RWC) was not different for cold-acclimated trees compared with the controls. Cold acclimation promoted stomatal closure at levels only observed in severely drought-stressed plants exposed to warm temperatures and where Ψstem and RWC are typically much lower than what was found in this study. Ψstem continued to decline the last 4 weeks of the experiment although air temperature, leaf ψS, RWC, stomatal conductance (gS), and transpiration were constant. The results of this experiment indicate that water relations of citrus during cold acclimation vary from those known to occur as a result of drought stress, which have implications for using traditional measures of plant water status in irrigation scheduling during winter.


2016 ◽  
Vol 66 (2) ◽  
pp. 328-331 ◽  
Author(s):  
Dorota Soltys-Kalina ◽  
Jarosław Plich ◽  
Danuta Strzelczyk-Żyta ◽  
Jadwiga Śliwka ◽  
Waldemar Marczewski

Author(s):  
Diah Rachmawati ◽  
Ni Luh Gde Mona Monika ◽  
Dan Ulfatul Masruroh

<p>Drought inhibits several physiological process and induces oxidative stress due to the enhanced production of reactive oxygen species (ROS) mainly in photosynthetic apparatus. Silicon (Si) is known to increase tolerance of rice against drought stress.  However, long period of intensive crop cultivation depleted the available soil Si by approximately 11-20%. Rice husk ash (RHA) is potential Si source. The objective of this research was to analyze the potency of RHA through pot experiment to observe: 1) internal water balanced; 2) integrity of cell membrane and  antioxidant content; and 3) production of tolerant cultivar ‘Segreng’ and sensitive one ‘Cempo merah’. Application of RHA was at level of 0, 4, and 8 tons ha-1. Drought stress was imposed by with holding water until soil water content reached 50% of field capacity (moderate stress) and 25% of field capacity (severe stress). Application of RHA significantly increased leaf relative water content and membrane stability index of rice ‘Segreng’ and ‘Cempo merah’. Tolerant cultivar ‘Segreng’ had better response than ‘Cempo merah’ as shown by greater leaf relative water content under moderate and severe stress. RHA application at level of 8 tons ha-1 increased index of membrane stability and level of antioxidant (AAred and α-tocopherol) which determine production of both rice ‘Segreng’ and ‘Cempo merah’ during drought.<br /><br />Keywords: antioxidant, field capacity, membrane stability, segreng cultivar, α-tocopherol</p>


2015 ◽  
Vol 48 (1) ◽  
pp. 107-114 ◽  
Author(s):  
S. Parvin ◽  
T. Javadi ◽  
N. Ghaderi

Abstract Drought is one of the critical environmental stresses that affect growth and development of plants. Plants are damaged directly and indirectly under drought stress. Increasing water stress tolerance in plants is crucial. The aim of this study was to investigate the effects of different water stress levels (-1, -5, and -10 bars) and paclobutrazol application (0 and 50 mg-1) on strawberry cv. Paros. According to analyses of variance there were significant effects of drought stress and paclobutrazol application on leaf area, leaf dry weight, leaf relative water content (RWC), cell membrane stability index (MSI), proline and protein content of leaves. Leaf area, leaf dry weight, leaf relative water content and cell membrane stability index decreased in drought stress, especially at -10 bars. Proline and protein contents were enhanced by increasing water stress levels. Paclobutrazol application increased leaf relative water content and cell membrane stability index, proline and protein contents of leaves. Leaf relative water content was 68.77% in -10 bars drought stress that increased to 79% in paclobutrazol treatment. Also, cell membrane stability index was 69.65% in severe drought stress and reached to 77% in paclobutrazol treatment. According to the results pacloburazol is a benefit substance to ameliorate drought stress effects in strawberry cv. Paros.


2022 ◽  
Vol 7 (1) ◽  
pp. 37-60
Author(s):  
Yenni ◽  
◽  
Mohd Hafiz Ibrahim ◽  
Rosimah Nulit ◽  
Siti Zaharah Sakimin ◽  
...  

<abstract> <p>Drought stress is one of the challenges that can affect the growth and the quality of strawberry. The study aims to determine the growth, biochemical changes and leaf gas exchange of three strawberry cultivars under drought stress. This study was conducted in a glasshouse at Indonesian Citrus and Subtropical Fruits Research Institute, Indonesia, from July-November 2018. The experiment was arranged in a factorial randomized completely block design (RCBD) with three replications and four water deficit (WD) levels [100% field capacity (FC)/well-watered), 75% of FC (mild WD), 50% of FC (moderate WD), and 25% of FC (severe WD)] for three strawberry cultivars (Earlibrite, California and Sweet Charlie). The results showed that total chlorophyll and anthocyanin contents (p ≤ 0.05) were influenced by the interaction effects of cultivars and water deficit. Whereas other parameters such as plant growth, transpiration rate (<italic>E</italic>), net photosynthesis (<italic>A</italic>), stomatal conductance (<italic>gs</italic>), leaf relative water content (LRWC), flowers and fruits numbers, proline content, length, diameter, weight and total soluble solid (TSS) of fruit were affected by water deficit. <italic>A</italic> had positive significant correlation with plant height (r = 0.808), leaf area (r = 0.777), fruit length (r = 0.906), fruit diameter (r = 0.889) and fruit weight (r = 0.891). Based on the results, cultivars affected LRWC, and also number of flowers and fruits of the strawberry. This study showed that water deficit decreased plant growth, chlorophyll content, leaf gas exchange, leaf relative water content, length, diameter and weight of fruit but enhanced TSS, anthocyanin, MDA, and proline contents. Increased anthocyanin and proline contents are mechanisms for protecting plants against the effects of water stress. California strawberry had the highest numbers of flowers and fruits, and also anthocyanin content. Hence, this cultivar is recommended to be planted under drought stress conditions. Among all water stress treatments, 75% of FC had the best results to optimize water utilization on the strawberry plants.</p> </abstract>


Author(s):  
Erum F H Kazi ◽  
Dr. Satish Kulkarni

Air pollution is one of major concerns in Pune City currently. Study highlights increase in Particulate matter from Vehicular sources & Urbanization in Karaj area is having harmful impact on the trees in the area. Leaf of Plant species such as Peepal( Ficusreligiosa),, Tamarind(Tamarindusindica), Rain tree( Samaneasaman), Ashoka( Saracaasoca), Manago( Mangiferaindica), Almond( Terminaliacatappa) , Banyan tree(Ficusbenghalensis) were selected and it was found that Ashoka( Saracaasoca), Mango tree( Mangiferaindica) showed Intermediate APTI whereas Peepal, Tamarind, Rain tree, Almond, Banyan tree were found to be Sensitive to pollution. KEYWORDS: Air Pollutants, APTI of plants, Total Chlorophyll, Ascorbic acid, p H of leaf, Relative water Content ( RWC)


Genetika ◽  
2012 ◽  
Vol 44 (1) ◽  
pp. 25-32 ◽  
Author(s):  
Ahmad Golparvar

Mode of gene action, heritability and determination of the effective breeding strategy for improvement of physiological and traits specifically in drought stress conditions is very important. Therefore, this study was conducted by using two drought susceptible and tolerant wheat cultivars. Cultivars Sakha8 (tolerant) and Pishtaz (susceptible) as parents along with F1, F2, BC1 and BC2 generations were sown in a randomized complete block design with three replications in drought stress conditions. Results of analysis of variance indicated significant difference between generations as well as degree of dominance revealed over-dominance for the both traits. Fitting simple additive-dominance model designated that this model was not able to account for changes of traits relative water content and mean of grain filling rate. It was revealed that m-d-h-i-j model for relative water content and m-d-h-i model for mean of grain filling rate are the best models. Estimation of heritability and mode of gene action indicated that selection for improvement of traits studied in stress condition and specifically in early generations have medium genetic gain. In conclusion, grain filling rate is better than relative water content as indirect selection criteria to improve plant grain yield in drought stress condition.


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