scholarly journals Effect of Geographical Location, Year, and Cultivar on Survival of Malus sp. Dormant Buds Stored in Vapors of Liquid Nitrogen

HortScience ◽  
2011 ◽  
Vol 46 (9) ◽  
pp. 1230-1234 ◽  
Author(s):  
Maria M. Jenderek ◽  
Phil Forsline ◽  
Joseph Postman ◽  
Ed Stover ◽  
David Ellis
Keyword(s):  
Cold Hardiness ◽  
Geographical Location ◽  
Germplasm Collections ◽  
Dormant Bud ◽  
Apple Cultivars ◽  
Woody Crop ◽  
Crop Germplasm ◽  
Winter Buds ◽  
Cryopreservation Method ◽  
Geographical Locations

Clonal woody crop germplasm collections often originate and are grown in distinct geographical locations. Because the degree of cold-hardiness is known to be a factor in the successful use of dormant bud cryopreservation for Malus, it was suggested that material from relatively warmer climates would not respond to cryopreservation as well as material from colder environments. To test this hypothesis, the effect of growing provenance on cryosurvival of dormant buds from three Malus (apple) cultivars grown in three locations (Geneva, NY; Davis, CA; and Corvallis, OR) was tested in 3 consecutive years. Dormant winter buds were harvested at the three locations, cryopreserved, and bud viability was tested by grafting. The collective 3-year mean viability for cryopreserved dormant apple buds for the three locations ranged from 63% to 81% of the buds surviving with the highest survival from the Corvallis site; however, the Geneva twigs were exposed to the lowest preharvest temperature. These results suggest that the temperature at the growing location may not hinder application of the dormant bud cryopreservation method with Malus to the extent previously speculated.

scholarly journals Cryopreservation of Cold-tender Apple Germplasm

1999 ◽  
Vol 124 (6) ◽  
pp. 612-618 ◽  
Author(s):  
Manfredo J. Seufferheld ◽  
Cecil Stushnoff ◽  
Philip L. Forsline ◽  
Gerardo H. Terrazas Gonzalez
Keyword(s):  
Cold Acclimation ◽  
Cold Hardiness ◽  
Meristematic Tissue ◽  
Tissue Levels ◽  
Malus Sylvestris ◽  
Dormant Bud ◽  
Golden Delicious ◽  
Apple Cultivars ◽  
Forced Air ◽  
Cold Hardy

Unlike cold-hardy apple germplasm, dormant vegetative buds from cold-tender accessions require stabilization of meristematic tissue to protect against injury during desiccation and cryopreservation. Dormant buds of six apple cultivars [Malus sylvestris (L.) Mill. var. domestica (Borkh.) Mansf. `Cox's Orange Pippin', `Einshemer', `Golden Delicious', `Jonagold', `K-14', and `Mutsu'] collected at specific intervals in 1993, 1994, and 1995 at Geneva, N.Y., were stabilized by encapsulation in 5% alginate, treated with step-wise imbibition of 0.5 to 1.0 m sucrose and 0.2 m raffinose solution, and desiccated with forced air at 0 °C. Sugar-alginate stabilization reduced injury during desiccation, increased cold-hardiness of the six cold-tender cultivars frozen to -30 °C, and improved recovery following cryopreservation of buds collected before optimal cold acclimation was attained. Sucrose tissue levels did not increase following stabilization treatment, but levels of glucose and fructose, and of an unknown disaccharide increased. This procedure used nontoxic cryoprotectants, and has potential to expand the scope of dormant bud cryopreservation to include cold-tender apple germplasm.

scholarly journals Low Temperature Tolerance of Apple Cultivars of Different Ploidy at Different Times of the Winter

2017 ◽  
Vol 71 (3) ◽  
pp. 127-131 ◽  
Author(s):  
Zoya Ozherelieva ◽  
Evgeny Sedov
Keyword(s):  
Frost Resistance ◽  
Cold Hardiness ◽  
Temperature Tolerance ◽  
Late Winter ◽  
Low Temperature Tolerance ◽  
Russian Research Institute ◽  
Apple Cultivars ◽  
Artificial Freezing ◽  
In The Beginning ◽  
Winter Buds

AbstractArtificial freezing was used to evaluate diploid and triploid apple cultivars from the All Russian Research Institute of Fruit Crop Breeding at Orel throughout three winters. The studied apple varieties were developed by breeder E. N. Sedov and cytological analysis was carried out by cytologist G. A. Sedysheva. In early winter, all cultivars exhibited high tolerance to cold. In mid-winter buds and wood were severely damaged, while bark was more resistant for most cultivars. Basic components of hardiness were estimated: component I - frost resistance at -25 °C in the beginning of winter; component II - maximum value of frost resistance at -40 °C developed by plants during hardening; component III - ability to retain the hardened condition at -25 °C after a period of three-day thaw at +2 °C; and component IV - the ability to restore frost resistance at -30 °C after repeated hardening and three-day thaw at +2 °C. During late-winter thaws, buds suffered from frosts, while the bark and wood retained frost hardiness. Late in winter all cultivars demonstrated high resistance to repeated frosts. Triploid cultivars exhibited the highest level of cold hardiness of vegetative buds, bark and wood of annual shoots throughout the winter; these cultivars included ‘Zhilinskoye’, ‘Vavilovskoye’, ‘Osipovskoye’, ‘Patriot’, ‘Sinap Orlovski’, ‘Spasskoye’, ‘Turgenevskoye’, and diploids ‘Bolotovskoye’, ‘Sokovinka’, and ‘’Ranneye Aloye’.

scholarly journals The Perspective of Croatian Old Apple Cultivars in Extensive Farming for the Production of Functional Foods

Foods ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 708
Author(s):  
Boris Duralija ◽  
Predrag Putnik ◽  
Dora Brdar ◽  
Anica Bebek Markovinović ◽  
Sandra Zavadlav ◽  
...  
Keyword(s):  
Functional Foods ◽  
Geographical Location ◽  
Nutritional Composition ◽  
Climatic Conditions ◽  
Biologically Active ◽  
Biological Potential ◽  
Apple Cultivars ◽  
The Republic ◽  
Processed Form ◽  
Extensive Farming

The Republic of Croatia has a long tradition of fruit growing due to its geographical location, climatic conditions, and high quality of fruit crops, especially apple fruits. Apples can be used for the formulation of functional foods either in processed form (e.g., juice), or as a by-product (e.g., apple pomace). However, there is a growing demand for functional foods derived from ancient and traditional plant sources as they are recognized as a very valuable source of health-promoting bioactive ingredients. Similarly, old apple cultivars (Malus domestica Borkh.) are characterized by good morphological and pomological properties, less need for chemicals during cultivation and the higher share of biologically active compounds (BACs) with better sensory acceptability compared to commercial cultivars. However, their nutritional and biological potential is underestimated, as is their ability to be processed into functional food. The importance in preserving old apple cultivars can also be seen in their significance for improving the nutritional composition of other apple cultivars through innovative cultivation strategies, and therefore old local apple cultivars could be of great importance in future breeding programs.

scholarly journals Bayesian Geographical Multi-Dimensional Scaling

2019 ◽  
Vol 1 ◽  
pp. 1-2
Author(s):  
Hayato Nishi ◽  
Yasushi Asami
Keyword(s):  
Three Dimensional ◽  
Geographical Location ◽  
Point Of View ◽  
Statistical Point ◽  
Dimensional Scaling ◽  
Regional Features ◽  
Geographical Distances ◽  
Strong Relation ◽  
Geographical Locations ◽  
Multi Dimensional Scaling

<p><strong>Abstract.</strong> Multi-dimensional scaling (MDS) is a popular method of visualizing the similarity of individuals in a dataset. When dissimilarities between individuals in a dataset are measured, MDS projects these individuals into the (typically two- or three-dimensional) map. In this map, because similar individuals are projected to be close to one another, distances between individuals correspond to their dissimilarities. In other words, MDS makes a similarity map of a dataset.</p><p>Some of the dissimilarities and distances have a strong relation to the geographical location. For example, time distances are similar to geographical distances, and regional features will be similar if the regions are close together. Therefore, it will be useful to compare the MDS projection and geographical locations. However, because MDS projection is not concerned with the rotation, parallel translation, and similarity expansion, it might be difficult to compare the projection to the actual geographical locations. When geographically related similarities are visualized, projected locations should be bound to the geographical locations.</p><p>In this article, we propose Bayesian Geographical Multidimensional Scaling (BGMDS), in which geographical restrictions of projections are given from a statistical point of view. BGMDS gives not only geographically bound projections, but also incorporates the uncertainty of the projections.</p>

scholarly journals Candidate gene mapping identifies genomic variations in the fire blight susceptibility genes HIPM and DIPM across the Malus germplasm

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Richard Tegtmeier ◽  
Valerio Pompili ◽  
Jugpreet Singh ◽  
Diego Micheletti ◽  
Katchen Julliany Pereira Silva ◽  
...  
Keyword(s):  
Candidate Gene ◽  
Fire Blight ◽  
Gene Families ◽  
Genomic Diversity ◽  
Bacterial Disease ◽  
Nucleotide Polymorphisms ◽  
Germplasm Collections ◽  
Apple Cultivars ◽  
Candidate Gene Mapping ◽  
In Fire

Abstract Development of apple (Malus domestica) cultivars resistant to fire blight, a devastating bacterial disease caused by Erwinia amylovora, is a priority for apple breeding programs. Towards this goal, the inactivation of members of the HIPM and DIPM gene families with a role in fire blight susceptibility (S genes) can help achieve sustainable tolerance. We have investigated the genomic diversity of HIPM and DIPM genes in Malus germplasm collections and used a candidate gene-based association mapping approach to identify SNPs (single nucleotide polymorphisms) with significant associations to fire blight susceptibility. A total of 87 unique SNP variants were identified in HIPM and DIPM genes across 93 Malus accessions. Thirty SNPs showed significant associations (p < 0.05) with fire blight susceptibility traits, while two of these SNPs showed highly significant (p < 0.001) associations across two different years. This research has provided knowledge about genetic diversity in fire blight S genes in diverse apple accessions and identified candidate HIPM and DIPM alleles that could be used to develop apple cultivars with decreased fire blight susceptibility via marker-assisted breeding or biotechnological approaches.

Alignment of Chartered SUCs Information Technology Program to the Commission on Higher Education (CHED) Policies and Standards

2015 ◽  
Vol 13 (1) ◽  
Author(s):  
Unife O. Cagas
Keyword(s):  
Higher Education ◽  
Information Technology ◽  
Technology Education ◽  
Statistical Treatment ◽  
Geographical Location ◽  
Survey Questionnaire ◽  
Global Challenge ◽  
Information Technology Education ◽  
Excellence In Education ◽  
Geographical Locations

Considering the global challenge of achieving excellence in education, it is imperative that the Philippine Higher Education Institutions should align to the standards set by the accrediting bodies. The study aimed to determine the alignment of chartered SUCs IT program in the Caraga Region to CHED Policies and Standards. Employing mixed methods, the study considered three types of instruments: survey questionnaire, interview schedule, and observation/ocular visit checklist. The survey questionnaire and observation/ocular visit checklist, which was based on CMO no. 53, series 2006 for Information Technology Education Programs, were used. Data were subjected to statistical treatment as frequency, mean, standard deviation and Analysis of Variance (ANOVA). The results revealed that in terms of alignment percentage by geographical location, generally, the areas – curriculum outline, instructional standards, admission and residence requirements, garnered the highest alignment percentage of 100.00% in four geographical locations. The study, therefore, concludes that one geographical location is not significantly better than the other, or that one is not significantly more aligned than other geographical locations. It is recommended that chartered SUC administrations should continuously upgrade and update the school’s physical facilities and equipment in conformity to the CHED standards and other recognized accrediting agencies that require standardization to support the IT faculty in the delivery of instruction.   Keywords - Technology Education, alignment, chartered SUCs, CHED policies and standards, descriptive design, Philippines

scholarly journals Diversity of Intestinal Microbiota in Coilia ectenes from Lake Taihu, China

2017 ◽  
Vol 12 (1) ◽  
pp. 315-325 ◽  
Author(s):  
Jinrong Duan ◽  
Dongpo Xu ◽  
Kai Liu ◽  
Yanfeng Zhou ◽  
Pao Xu
Keyword(s):  
Chlorophyll A ◽  
Total Nitrogen ◽  
Intestinal Microbiota ◽  
High Throughput Sequencing ◽  
Lake Taihu ◽  
Geographical Location ◽  
Operational Taxonomic Units ◽  
Coilia Ectenes ◽  
Geographical Locations

AbstractTo investigate the community structure and species composition of intestinal microbiota in Coilia ectenes, sixty-seven samples were collected from Lake Taihu in China. The intestinal microbiota of the C. ectenes were identified by the V4 of the 16S ribosomal RNA gene using high-throughput sequencing. Furthermore, the water quality of different sampling sites was also evaluated. A total of 53 phyla and 730 classified genera were found in all the samples. The eight dominant phyla Planctomycetes, Firmicutes, Proteobacteria, Bacteroidetes, Cyanobacteria, Crenarchaeota, Actinobacteria, and Verrucomicrobia were included. The intestinal microbiota compositions of the C. ectenes obtained from the same location presented more similar profiles, and the intestinal microbiota compositions of the C. ectenes from different geographical locations showed considerable differences. The operational taxonomic units (OTUs) abundance of the intestinal microbiota was significantly correlated with both the concentrations of total nitrogen and chlorophyll-a of the aquatic environment (p<0.05). Geographical location was an important determinant for the fish’s intestinal microbiota composition. The intestinal microbiota of C. ectenes would be affected by the concentrations of total nitrogen and chlorophyll-a in the water. These findings achieve a basic understanding of fish’s gut microbiota, and are helpful for the protection of fish resources in Lake Taihu and provided the cornerstone to sustainable utlization of C. ectenes.

scholarly journals Molecular Variation of Satellite DNAβ Molecules Associated with Malvastrum yellow vein virus and Their Role in Pathogenicity

2008 ◽  
Vol 74 (6) ◽  
pp. 1909-1913 ◽  
Author(s):  
Wei Guo ◽  
Tong Jiang ◽  
Xian Zhang ◽  
Guixin Li ◽  
Xueping Zhou
Keyword(s):  
Petunia Hybrida ◽  
Yunnan Province ◽  
Geographical Origin ◽  
Geographical Location ◽  
Yellow Vein ◽  
Molecular Variation ◽  
Infectious Clones ◽  
Malvastrum Coromandelianum ◽  
Geographical Locations ◽  
Limited Sequence

ABSTRACT Previous studies have found that the diversity of begomovirus-associated DNAβ satellites is related to host and geographical origin. In this study, we have cloned and sequenced 20 different isolates of DNAβ molecules associated with Malvastrum yellow vein virus (MYVV) isolated from Malvastrum coromandelianum plants in different geographical locations of Yunnan Province, China. Analyses of their molecular variation indicate that the satellites are clustered together according to their geographical location but that they have only limited sequence diversity. Infectivity tests using infectious clones of MYVV and its associated DNAβ molecule indicate that MYVV DNAβ is indispensable for symptom induction in Nicotiana benthamiana, N. glutinosa, Petunia hybrida, and M. coromandelianum plants. Furthermore, we showed that MYVV interacts functionally with heterologous DNAβ molecules in N. benthamiana plants.

scholarly journals Development of a minimal KASP marker panel for distinguishing genotypes in apple collections

PLoS ONE ◽  
2020 ◽  
Vol 15 (11) ◽  
pp. e0242940
Author(s):  
Mark Winfield ◽  
Amanda Burridge ◽  
Matthew Ordidge ◽  
Helen Harper ◽  
Paul Wilkinson ◽  
...  
Keyword(s):  
Snp Markers ◽  
Nucleotide Polymorphism ◽  
Accurate Identification ◽  
Crop Species ◽  
Single Nucleotide ◽  
Germplasm Collections ◽  
Large Numbers ◽  
Small Set ◽  
Apple Cultivars ◽  
Kasp Marker

Accurate identification of named accessions in germplasm collections is extremely important, especially for vegetatively propagated crops which are expensive to maintain. Thus, an inexpensive, reliable, and rapid genotyping method is essential because it avoids the need for laborious and time-consuming morphological comparisons. Single Nucleotide Polymorphism (SNP) marker panels containing large numbers of SNPs have been developed for many crop species, but such panels are much too large for basic cultivar identification. Here, we have identified a minimum set of SNP markers sufficient to distinguish apple cultivars held in the English and Welsh national collections providing a cheaper and automatable alternative to the markers currently used by the community. We show that SNP genotyping with a small set of well selected markers is equally efficient as microsatellites for the identification of apple cultivars and has the added advantage of automation and reduced cost when screening large numbers of samples.

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