Eligible strategies of drought response to improve drought resistance in woody crops: a mini-review

Drought is the main abiotic stress that negatively affects the crop yield. Due to the rapid climate change, actual plant defence mechanisms may be less effective against increased drought stress and other related or co-occurring abiotic stresses such as salt and high temperature. Thus, genetic engineering approaches may be an important tool for improving drought tolerance in crops. This mini-review focuses on the responses to drought stress of the woody crop species Olea europaea and Citrus sp., selecting in particular five main strategies adopted by plants in response to drought stress: aquaporin (AQPs) expression, antioxidant activity, ABA signalling, and trehalose and proline accumulation. Transgenic studies on both the herbaceous Arabidopsis and woody Populus plant models showed an improvement in drought resistance with increasing expression of these drought-inducible genes. Outcomes from the present study suggest the overexpression of the gene families associated with AQPs and ABA biosynthesis, mainly involved in regulating water transport and in preventing water loss, respectively, as candidate targets for improving drought resistance; antioxidants-, trehalose- and proline-related genes remain valid candidates for resistance to a wider spectrum of abiotic stressors, including drought. However, the contribution of an increased stiffness of the modulus elasticity of leaf parenchyma cell walls to the rapid recovery of leaf water potential, delaying by this way the stress onset, is not a secondary aspect of the transgenic optimization, in particular for Olea cultivars.

Eucalyptus Amplifolia and Corymbia Torelliana in the Southeastern USA: Genetic Improvement and Potential Uses

Eucalyptus amplifolia and Corymbia torelliana genetic improvement has been conducted in the lower southeastern USA by UF and collaborators since 1980. The collective accomplishments in genetic resources and potential commercial uses are summarized. For example, fast-growing, freeze-resilient E. amplifolia seeds are provided by 1st and 2nd generation seedling seed orchards (SSO) and a 2nd generation clonal seed orchard (CSO), while C. torelliana seed are available from 1st and 2nd generation SSOs. Breeding values (BV) have been developed for guiding the deployment of improved genotypes. Collaborative genetic improvement of these species is ongoing, including testing E. amplifolia in 11 countries and development of hybrid clones. Short Rotation Woody Crop (SRWC) systems may increase productivity and extend uses beyond conventional mulchwood to products such as medium density fiberboard (MDF), biochar, and energywood, while other possible applications include honey production, windbreaks, dendroremediation, and carbon sequestration. C. torelliana may be paired with E. grandis in two-row windbreaks to maximum windbreak effectiveness and may sequester as much carbon as E. grandis.

The Tea Plant Leaf Cuticle: From Plant Protection to Tea Quality

Camellia sinensis (tea tree) is a perennial evergreen woody crop that has been planted in more than 50 countries worldwide; its leaves are harvested to make tea, which is one of the most popular nonalcoholic beverages. The cuticle is the major transpiration barrier to restrict nonstomatal water loss and it affects the drought tolerance of tea plants. The cuticle may also provide molecular cues for the interaction with herbivores and pathogens. The tea-making process almost always includes a postharvest withering treatment to reduce leaf water content, and many studies have demonstrated that withering treatment-induced metabolite transformation is essential to shape the quality of the tea made. Tea leaf cuticle is expected to affect its withering properties and the dynamics of postharvest metabolome remodeling. In addition, it has long been speculated that the cuticle may contribute to the aroma quality of tea. However, concrete experimental evidence is lacking to prove or refute this hypothesis. Even though its relevance to the abiotic and biotic stress tolerance and postharvest processing properties of tea tree, tea cuticle has long been neglected. Recently, there are several studies on the tea cuticle regarding its structure, wax composition, transpiration barrier organization, environmental stresses-induced wax modification, and structure–function relations. This review is devoted to tea cuticle, the recent research progresses were summarized and unresolved questions and future research directions were also discussed.

Short-term impact of crop diversification on soil carbon fluxes and balance in rainfed and irrigated woody cropping systems under semiarid Mediterranean conditions

Purpose Diversification practices such as intercropping in woody cropping systems have recently been proposed as a promising management strategy for addressing problems related to soil degradation, climate change mitigation and food security. In this study, we assess the impact of several diversification practices in different management regimes on the main carbon fluxes regulating the soil carbon balance under semiarid Mediterranean conditions. Methods The study was conducted in two nearby cropping systems: (i) a low input rainfed almond (Prunus dulcis Mill.) orchard cultivated on terraces and (ii) a levelled intensively irrigated mandarin (Citrus reticulata Blanco) orchard with a street-ridge morphology. The almond trees were intercropped with Capparis spinosa or with Thymus hyemalis While the mandarin trees were intercropped with a mixture of barley and vetch followed by fava bean. Changes caused by crop diversifications on C inputs into the soil and C outputs from the soil were estimated. Results Crop diversification did not affect soil organic carbon stocks but did affect the carbon inputs and outputs regulating the soil carbon balance of above Mediterranean agroecosystems. Crop diversification with perennials in the low-input rainfed woody crop system significantly improved the annual soil C balance in the short-term. However, crop diversification with annual species in the intensively managed woody crop system had not effect on the annual soil C balance. Conclusions Our results highlight the potential of intercropping with perennials in rainfed woody crop systems for climate change mitigation through soil carbon sequestration.

Monitoring Crop Evapotranspiration and Transpiration/Evaporation Partitioning in a Drip-Irrigated Young Almond Orchard Applying a Two-Source Surface Energy Balance Model

Encouraged by the necessity to better understand the water use in this woody crop, a study was carried out in a commercial drip-irrigated young almond orchard to quantify and monitor the crop evapotranspiration (ETc) and its partitioning into tree canopy transpiration (T) and soil evaporation (E), to list and analyze single and dual crop coefficients, and to extract relationships between them and the vegetation fractional cover (fc) and remote-sensing-derived vegetation indices (VIs). A Simplified Two-Source Energy Balance (STSEB) model was applied, and the results were compared to ground measurements from a flux tower. This study comprises three consecutive growing seasons from 2017 to 2019, corresponding to Years 2 to 4 after planting. Uncertainties lower than 50 W m−2 were obtained for all terms of the energy balance equation on an instantaneous scale, with average estimation errors of 0.06 mm h−1 and 0.6 mm d−1, for hourly and daily ETc, respectively. Water use for our young almond orchard resulted in average mid-season crop coefficient (Kc mid) values of 0.30, 0.33, and 0.45 for the 2017, 2018, and 2019 growing seasons, corresponding to fc mean values of 0.21, 0.35, and 0.39, respectively. Average daily evapotranspiration for the same periods resulted in 1.7, 2.1, and 3.2 mm d−1. The results entail the possibility of predicting the water use of any age almond orchards by monitoring its biophysical parameters.

Evaluation of Established Methods for DNA Extraction and Primer Pairs Targeting 16S rRNA Gene for Bacterial Microbiota Profiling of Olive Xylem Sap

Next-generation sequencing has revolutionized our ability to investigate the microbiota composition of diverse and complex environments. However, a number of factors can affect the accuracy of microbial community assessment, such as the DNA extraction method, the hypervariable region of 16S rRNA gene targeted, or the PCR primers used for amplification. The aim of this study was to assess the influence of commercially available DNA extraction kits and different primer pairs to provide a non-biased vision of the composition of bacterial communities present in olive xylem sap. For that purpose, branches from “Picual” and “Arbequina” olive cultivars were used for xylem sap extraction using a Scholander chamber device. The DNA extraction protocol significantly affected xylem sap bacterial community assessment. That resulted in significant differences in alpha (Richness) and beta diversity (UniFrac distances) metrics among DNA extraction protocols, with the 12 DNA extraction kits evaluated being clustered in four groups behaving differently. Although the core number of taxa detected by all DNA extraction kits included four phyla, seven classes, 12 orders, 16 or 21 families, and 12 or 14 genera when using the Greengenes or Silva database for taxonomic assignment, respectively, some taxa, particularly those identified at low frequency, were detected by some DNA extraction kits only. The most accurate depiction of a bacterial mock community artificially inoculated on sap samples was generated when using the PowerPlant DNA extraction kit, the combination of 799F/1193R primers amplifying the hypervariable V5–V7 region, and the Silva 132 database for taxonomic assignment. The DESeq2 analysis displayed significant differences among genera abundance between the different PCR primer pairs tested. Thus, Enterobacter, Granulicatella, Prevotella, and Brevibacterium presented a significant higher abundance in all PCR protocols when compared with primer pair 799F/1193R, while the opposite was true for Pseudomonas and Pectobacterium. The methodological approach followed in this study can be useful to optimize plant-associated microbiome analysis, especially when exploring new plant niches. Some of the DNA extraction kits and PCR primers selected in this study will contribute to better characterize bacterial communities inhabiting the xylem sap of olives or other woody crop species.

Determinant factors in olive oil accumulation for optimizing harvest time in a context of climate change

<p>Olive is a woody crop extended over 10 Mha around the world (FAOSTAT, 2019), being Spain the country with the largest area (2.7 Mha). Andalusia is located in the South of Spain, with 1.6 Mha cultivated with olive trees, most of them (around 90%) dedicated to olive oil production (MAPA, 2020). This region is characterized by a great diversity of weather conditions. This diversity greatly affects important agronomic parameters of olive as the pattern of oil accumulation. This influence is different depending on the cultivar considered. In addition, this pattern of oil accumulation is a key aspect since is the most relevant trait determining the optimal harvest time. For that reason, in the present study, the relative influence of cultivar and environment, and their interaction, have been evaluated for the full pattern of oil accumulation.</p><p>This study was carried out in four locations of Andalusia covering a wide range of weather conditions, and where olive trees are well established or under expansion: Antequera (Málaga), Córdoba, Úbeda (Jaén) and Gibraleón (Huelva). In 2008, five cultivars were planted in a randomized complete block design consisting in four blocks and four trees per elementary plot: Arbequina, Hojiblanca, Koroneiki, Picual and Sikitita-3 (a new registered cultivar from the olive breeding program developed by the University of Córdoba and IFAPA). The first two locations were monitored in 2018 and 2020 while the other two locations were monitored only during 2020 campaign. Fruits samples were collected periodically, starting 4 weeks after full bloom until the oil accumulation was finished. Then, in the laboratory, fruits’ oil content was measured by nuclear magnetic resonance.</p><p>Results show sigmoid patterns regarding fruit oil accumulation and dry basis along each campaign in all genotypes, locations and years. There were significant differences of maximum olive oil accumulation among genotypes, recording the genotype Sikitita-3 the maximum ones. Furthermore, a significant genotype-environment interaction was also found for these. These results have relevant consequences regarding the selection of the optimal harvest time, to accomplish a desired balance between maximum oil accumulation and quality indicators which require early harvest dates.</p><p> </p><p>References:</p><p>FAOSTAT, 2019. Food and Agriculture Organization of the United Nations. FAOSTAT database available at http://www.fao.org/faostat/en/#data. Last accessed 12 January 2020.</p><p>MAPA, 2020. Ministry of Agriculture, Fisheries and Food. Survey of surfaces and crop yields 2020 available at https://www.mapa.gob.es/es/estadistica/temas/estadisticas-agrarias/agricultura/esyrce/. Last accessed 12 January 2020.</p>

Epigenetic Changes and Transcriptional Reprogramming Upon Woody Plant Grafting for Crop Sustainability in a Changing Environment

Plant grafting is an ancient agricultural practice widely employed in crops such as woody fruit trees, grapes, and vegetables, in order to improve plant performance. Successful grafting requires the interaction of compatible scion and rootstock genotypes. This involves an intricate network of molecular mechanisms operating at the graft junction and associated with the development and the physiology of the scion, ultimately leading to improved agricultural characteristics such as fruit quality and increased tolerance/resistance to abiotic and biotic factors. Bidirectional transfer of molecular signals such as hormones, nutrients, proteins, and nucleic acids from the rootstock to the scion and vice versa have been well documented. In recent years, studies on rootstock-scion interactions have proposed the existence of an epigenetic component in grafting reactions. Epigenetic changes such as DNA methylation, histone modification, and the action of small RNA molecules are known to modulate chromatin architecture, leading to gene expression changes and impacting cellular function. Mobile small RNAs (siRNAs) migrating across the graft union from the rootstock to the scion and vice versa mediate modifications in the DNA methylation pattern of the recipient partner, leading to altered chromatin structure and transcriptional reprogramming. Moreover, graft-induced DNA methylation changes and gene expression shifts in the scion have been associated with variations in graft performance. If these changes are heritable they can lead to stably altered phenotypes and affect important agricultural traits, making grafting an alternative to breeding for the production of superior plants with improved traits. However, most reviews on the molecular mechanisms underlying this process comprise studies related to vegetable grafting. In this review we will provide a comprehensive presentation of the current knowledge on the epigenetic changes and transcriptional reprogramming associated with the rootstock–scion interaction focusing on woody plant species, including the recent findings arising from the employment of advanced—omics technologies as well as transgrafting methodologies and their potential exploitation for generating superior quality grafts in woody species. Furthermore, will discuss graft—induced heritable epigenetic changes leading to novel plant phenotypes and their implication to woody crop improvement for yield, quality, and stress resilience, within the context of climate change.