The Differential Response of Two Chrysanthemum Cultivars to Shading: Photosynthesis, Chloroplast, and Sieve Element-companion Cell Ultrastructure

The response to reduced light intensity of two contrasting cultivars Puma Sunny (shade intolerant) and Gongzi (shade tolerant) was characterized in terms of plant height, the root/shoot ratio, photosynthetic capacity, and the morphology and ultrastructure of their chloroplasts and phloem companion cells. The initial response to shading of cultivar Puma Sunny plants was to extend their stems, and while the equivalent response of cultivar Gongzi was less marked. Shading depressed the maximum relative electron transport rate (rETR) in both cultivars, and while the efficiency of light capture in cultivar Puma Sunny was compromised by shading, this was not the case for cultivar Gongzi. Low levels of incident light inhibited the formation of starch grains in the chloroplast and increased the volume of interspace between the grana lamellae. In cultivar Puma Sunny, but less so in cultivar Gongzi, the chloroplasts became more slender and the stroma lamellae more swollen. Adjusting chloroplast morphology by developing extra layers of grana lamellae and maintaining the integrity of the phloem companion cells are both adaptations which help make ‘Gongzi’ a more shade-tolerant cultivar.

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A double-digitising method for building 3D virtual trees with non-planar leaves: application to the morphology and light-capture properties of young beech trees (Fagus sylvatica)

Functional Plant Biology ◽

10.1071/fp08051 ◽

2008 ◽

Vol 35 (10) ◽

pp. 1059 ◽

Cited By ~ 17

Author(s):

Jean-Christophe Chambelland ◽

Mathieu Dassot ◽

Boris Adam ◽

Nicolas Donès ◽

Philippe Balandier ◽

...

Keyword(s):

Fagus Sylvatica ◽

Leaf Morphology ◽

Incident Light ◽

Light Availability ◽

Laser Scanner ◽

Light Interception ◽

Shape Parameters ◽

Fagus Sylvatica L ◽

Cloud Of Points ◽

Light Capture

We developed a double-digitising method combining a hand-held electromagnetic digitizer and a non-contact 3D laser scanner. The former was used to record the positions of all leaves in a tree and the orientation angles of their lamina. The latter served to obtain the morphology of the leaves sampled in the tree. As the scanner outputs a cloud of points, software was developed to reconstruct non-planar (NP) leaves composed of triangles, and to compute numerical shape parameters: midrib curvature, torsion and transversal curvature of the lamina. The combination of both methods allowed construction of 3D virtual trees with NP leaves. The method was applied to young beech trees (Fagus sylvatica L.) from different sunlight environments (from 1 to 100% incident light) in a forest in central France. Leaf morphology responded to light availability, with a more bent shape in well-lit leaves. Light interception at the leaf scale by NP leaves decreased from 4 to 10% for shaded and sunlit leaves compared with planar leaves. At the tree scale, light interception by trees made of NP leaves decreased by 1 to 3% for 100% to 1% light, respectively.

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Enhanced Relative Electron Transport Rate Contributes to Increased Photosynthetic Capacity in Autotetraploid Pak Choi

Plant and Cell Physiology ◽

10.1093/pcp/pcz238 ◽

2020 ◽

Vol 61 (4) ◽

pp. 761-774 ◽

Cited By ~ 2

Author(s):

Changwei Zhang ◽

Huiyu Wang ◽

Yuanyuan Xu ◽

Shuning Zhang ◽

Jianjun Wang ◽

...

Keyword(s):

Electron Transport ◽

Powdery Mildew Resistance ◽

Photosynthetic Activity ◽

Photosynthetic Capacity ◽

Electron Transport Rate ◽

Ectopic Expression ◽

Transport Rate ◽

Pak Choi ◽

Relative Electron Transport Rate ◽

Relative Electron

Abstract Autopolyploids often show growth advantages over their diploid progenitors because of their increased photosynthetic activity; however, the underlying molecular basis of such mechanism remains elusive. In this study, we aimed to characterize autotetraploid pak choi (Brassica rapa ssp. chinensis) at the physiological, cellular and molecular levels. Autotetraploid pak choi has thicker leaves than its diploid counterparts, with relatively larger intercellular spaces and cell size and greater grana thylakoid height. Photosynthetic data showed that the relative electron transport rate (rETR) was markedly higher in autotetraploid than in diploid pak choi. Transcriptomic data revealed that the expressions of genes involved in ‘photosynthesis’ biological process and ‘thylakoids’ cellular component were mainly regulated in autotetraploids. Overall, our findings suggested that the increased rETR in the thylakoids contributed to the increased photosynthetic capacity of autotetraploid leaves. Furthermore, we found that the enhanced rETR is associated with increased BrPetC expression, which is likely altered by histone modification. The ectopic expression of BrPetC in Arabidopsis thaliana led to increased rETR and biomass, which were decreased in BrPetC-silenced pak choi. Autotetraploid pak choi also shows altered hormone levels, which was likely responsible for the increased drought resistance and the impaired powdery mildew resistance of this lineage. Our findings further our understanding on how autotetraploidy provides growth advantages to plants.

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Influence of Low Light on the Growth and Development of Vallisneria natans Seedlings

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.295-298.62 ◽

2013 ◽

Vol 295-298 ◽

pp. 62-68 ◽

Cited By ~ 3

Author(s):

Qiang Li ◽

Yun Cheng Xie

Keyword(s):

Weight Ratio ◽

Photochemical Quenching ◽

Low Light ◽

Vallisneria Natans ◽

Relative Electron Transport Rate ◽

Leaf Formation ◽

Relative Electron ◽

Macrophyte Growth ◽

Non Photochemical Quenching ◽

Photochemical Quantum Yield

To research influence of low light on macrophyte growth, seedlings of Vallisneria natans were transplanted into the waters with 5%, 1%, 0.5% and 0.1% full sunlights, respectively. The results show that germination rates are significantly inhibited with the experiment. In first 20 days leaf formation of new plants aren’t inhibited, and their leaf elongation are promoted. Since then, them of old plants and new plants are remarkably inhibited with the experiment. Though maximum photochemical quantum yield (Fv/Fm), relative electron transport rate (ETR) and non-photochemical quenching (NPQ) of old plants reduce significantly on low light conditions, but V. natans still hsa a certain degree of photoasynthetic capacity on the 80th day. Fresh weight ratio of roots and stems increase with decreasing light intensity. However, it exhibits an opposite trend for leaves. Thus, V. natans has a strong ability of low-light (≤1% full sunlight) tolerance.

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Influence of Photoperiods on Microalgae Biofilm: Photosynthetic Performance, Biomass Yield, and Cellular Composition

Energies ◽

10.3390/en12193724 ◽

2019 ◽

Vol 12 (19) ◽

pp. 3724 ◽

Cited By ~ 3

Author(s):

Xinru Zhang ◽

Hao Yuan ◽

Libo Guan ◽

Xinyu Wang ◽

Yi Wang ◽

...

Keyword(s):

Biomass Yield ◽

Low Cost ◽

Biomass Productivity ◽

Chlorella Pyrenoidosa ◽

Photosynthetic Performance ◽

Light Regimes ◽

Chlorella Sp ◽

Relative Electron Transport Rate ◽

Relative Electron ◽

Immense Potential

Microalgae have immense potential as biological sources to produce biofuels and high-value biomolecules. Biofilm-based microalgae cultivation has attracted much interest recently because of its high biomass productivity, reduced water use, and low cost of harvesting. This study aimed to understand the effect of photoperiod on three microalgae biofilms, including Nannochloris oculata, Chlorella sp., and Chlorella pyrenoidosa. The examined photoperiods were 3:3 s, 5:5 s, 30:30 min, 12:12 h (light-period-to-dark-period ratio), and continuous lighting. By determining the maximum quantum yield and relative electron transport rate of photosystem II, we found that photoperiods on the seconds scale improved photosynthetic performance of microalgae biofilm. Biomass yield and lipid content of these three microalgae cultured under the photoperiod with the seconds scale increased by 11%–24% and 7%–22%, respectively, compared with those cultured under continuous lighting. In addition, the photoperiods of 3:3 s, 5:5 s, 30:30 min, and 12:12 h were beneficial for protein synthesis. These results have important implications in establishing suitable light regimes for microalgae biofilm-based cultivation systems.

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Comparison of the Effects of Metamitron on Chlorophyll Fluorescence and Fruit Set in Apple and Peach

HortScience ◽

10.21273/hortsci.47.4.509 ◽

2012 ◽

Vol 47 (4) ◽

pp. 509-514 ◽

Cited By ~ 24

Author(s):

Steven J. McArtney ◽

John D. Obermiller ◽

Consuelo Arellano

Keyword(s):

Chlorophyll Fluorescence ◽

Fruit Set ◽

Chlorophyll Concentration ◽

Ionic Surfactant ◽

Additional Information ◽

Commercial Thinning ◽

Leaf Chlorophyll ◽

Relative Electron Transport Rate ◽

Negative Effect ◽

Relative Electron

The effects of foliar applications of the photosystem II (PSII) inhibitor metamitron on chlorophyll fluorescence and fruit set were compared in peach and apple trees. Metamitron increased dark-adapted chlorophyll fluorescence, measured as a reduction in Fv/Fm values, in both peaches and apples. Maximum suppression of the normalized ratio of variable fluorescence to maximum fluorescence (Fv/Fm) in peaches occurred 1 to 2 days after application and Fv/Fm values recovered by 7 days after treatment. The effects of metamitron on chlorophyll fluorescence were more persistent in apples compared with peaches. Fv/Fm values in apple declined within 2 days of treatment and did not start recovering until 5 days after treatment or longer. Concentrations of metamitron greater than 200 mg·L−1 were phytotoxic to peach leaves, reducing the leaf chlorophyll concentration as determined by SPAD measurements. At 300 mg·L−1, metamitron reduced fruit set in apple but not in peach. Inclusion of a non-ionic surfactant (Silwett L-77) with metamitron greatly increased its negative effect on Fv/Fm, quantum photosynthetic yield of PSII (ΦPSII), and relative electron transport rate (ETR). These results suggest that metamitron may be a useful thinner in apple but not in peach. Additional information is needed to understand how combining metamitron with existing thinning chemicals might enhance their activity. In particular, caution may be necessary if metamitron is applied as a tank mixture with commercial thinning products that have been formulated with a wetting agent.

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Response of tropical marine benthic diatoms exposed to elevated irradiance and temperature

10.5194/bg-2021-18 ◽

2021 ◽

Author(s):

Sazlina Salleh ◽

Andrew McMinn

Keyword(s):

Quantum Yield ◽

Electron Transport Rate ◽

High Tide ◽

Benthic Diatoms ◽

Photosynthetic Parameters ◽

Effective Quantum Yield ◽

Tidal Cycles ◽

Relative Electron Transport Rate ◽

Diatom Communities ◽

Relative Electron

Abstract. Shallow tropical marine environments are likely to experience future water temperatures that will challenge the ability of life to survive. Changes in temperature and irradiance during tidal cycles in the Tanjung Rhu estuary, Langkawi, Malaysia in 2007 did not significantly affect the benthic diatom communities, although, higher photosynthetic parameters, such as maximum relative electron transport rate (rETRmax), photosynthetic efficiency (α), maximum quantum yield (Fv/Fm) and effective quantum yield (∆F/Fm'), were recorded at high tide when the temperatures were lower. However, when benthic diatoms were experimentally exposed to irradiances of 1800 µmol photons m−2 s−1, they were only able to photosynthesize at temperatures

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The Fine Structure of Phloem Cells

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100119867 ◽

1985 ◽

Vol 43 ◽

pp. 632-635

Author(s):

James Cronshaw

Keyword(s):

Structural Studies ◽

High Concentration ◽

Long Distance ◽

Phloem Tissue ◽

Sieve Elements ◽

Long Distance Transport ◽

P Protein ◽

Companion Cells ◽

Electron Microscopical ◽

Distance Transport

Long distance transport in plants takes place in phloem tissue which has characteristic cells, the sieve elements. At maturity these cells have sieve areas in their end walls with specialized perforations. They are associated with companion cells, parenchyma cells, and in some species, with transfer cells. The protoplast of the functioning sieve element contains a high concentration of sugar, and consequently a high hydrostatic pressure, which makes it extremely difficult to fix mature sieve elements for electron microscopical observation without the formation of surge artifacts. Despite many structural studies which have attempted to prevent surge artifacts, several features of mature sieve elements, such as the distribution of P-protein and the nature of the contents of the sieve area pores, remain controversial.

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Effects of the pseudomonad phytotoxin coronatine on tomato leaf structure and ultrastructure

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100140683 ◽

1995 ◽

Vol 53 ◽

pp. 862-863

Author(s):

D.A. Palmer ◽

C.L. Bender

Keyword(s):

Pseudomonas Syringae ◽

Membrane Integrity ◽

Leaf Tissue ◽

Cell Number ◽

Cell Ultrastructure ◽

Response To Treatment ◽

Prominent Symptom ◽

Chlorophylls A And B ◽

Cell Dimensions ◽

Structure And Ultrastructure

Coronatine is a non-host-specific phytotoxin produced by several members of the Pseudomonas syringae group of pathovars. The toxin acts as a virulence factor in P. syringae pv. tomato, allowing the organism to multiply to a higher population density and develop larger lesions than mutant strains unable to produce the toxin. The most prominent symptom observed in leaf tissue treated with coronatine is an intense spreading chlorosis; this has been attributed to a loss of chlorophylls a and b in tobacco. Coronatine's effects on membrane integrity and cell ultrastructure have not been previously investigated. The present study describes changes in tomato leaves in response to treatment with purified coronatine, infection by a coronatine-producing strain of P. syringae pv. tomato, and infection by a cor" mutant.In contrast to H2O-treated tissue, coronatine-treated tissue showed a diffuse chlorosis extending approximately 5 mm from the inoculation site. Leaf thickness, cell number, and cell dimensions were similar for both healthy and coronatine-treated, chlorotic tissue; however, the epidermal cell walls were consistently thicker in coronatine-treated leaves (Figs, la and lb).

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