scholarly journals Effect of Low-temperature Storage on Sweet Cherry (Prunus avium L.) Pollen Quality

HortScience ◽  
2020 ◽  
Vol 55 (2) ◽  
pp. 258-260
Author(s):  
Akide Özcan
Keyword(s):  
Sweet Cherry ◽  
Prunus Avium ◽  
Pollen Viability ◽  
Pollen Quality ◽  
Low Temperature Storage ◽  
C Storage ◽  
Sweet Cherry Cultivars ◽  
Storage Temperatures ◽  
Temperature Storage

The objective of this study was to analyze the effects of low storage temperatures on the quality of pollen obtained from the sweet cherry cultivars 0900 Ziraat, Regina, Starks Gold, and Sweet Heart. The pollen was stored at 4 °C, –20 °C, and –80 °C for 12 months, and its viability and germinability were determined at 3-month intervals. The results demonstrated that the initial pollen viability varied between 73.62% and 79.37%, while pollen germinability varied between 41.24% and 53.56%. The percentage of pollen viability declined remarkably from the third to 12th month by almost 3% in 4 °C storage. The pollen viability for the other two storage temperatures (–20 °C and –80 °C) was greater than 50% by the end of the 12th month. It can be concluded that the pollen quality of these cultivars can be preserved sufficiently at temperatures less than –20 °C.

scholarly journals Genetic Diversity of Some Sweet Cherry Cultivars Based on Molecular Markers

2016 ◽  
Vol 73 (2) ◽  
pp. 153
Author(s):  
Ioana Virginia Berindean ◽  
Elena Tămaş ◽  
Oana Maria Toderic ◽  
Ioan Zagrai
Keyword(s):  
Tree Species ◽  
Rapd Markers ◽  
Sweet Cherry ◽  
Prunus Avium ◽  
Molecular Level ◽  
Genetic Relationships ◽  
Pcr Amplification ◽  
High Level ◽  
Sweet Cherry Cultivars

Sweet cherry (Prunus avium L.), originated around the Caspian and Black Sea, is an important fruit tree species of economic interest, and hence, breeding and conservation are requested (. Genetic analysis at the molecular level can be used effectively to study molecular polymorphism existing between intraspecific and interspecific tree species and phylogenetic relationships between them and their hybrids. The purpose of this study was to characterize and determine genetic relationships among the sweet cherry native genotypes belonging to Fruit Research & Development Station Bistrita, Romania, using RAPD markers. To eliminate the existence of possible synonyms from national romanian collection, we collect four Van cultivars, from four different national collection. For molecular analysis of the 16 varieties of sweet cherry were considered 13 RAPD primers selected from the literature. They were later used to determine the genetic variability at the molecular level using PAST program, and the dendrogram was generated based on Jaccard’s genetic distance. The dendrogram constructed by PAST software. The quantity and quality of the DNA obtained was suitable to achieve PCR amplification step. Only seven out of the 13 RAPD primers have generate polymorphic bands. The rest of seven were monomorphics. The most polymorphic primer was OPB10 which generated 11 bands from which 100% were polymorphic.Seven RAPD primers generated a high level of polymorphism which allowed to divide these cherry varieties into two groups according to their genetic geographical origin and the pedigree.

scholarly journals Low Temperature Storage and in vitro Pollen Germination of Selected Spring Wheat Accessions

2017 ◽  
Vol 9 (9) ◽  
pp. 1
Author(s):  
Bahram Baninasab ◽  
Mirko Tabori ◽  
Junjie Yu ◽  
Yuxue Zhang ◽  
Xuelian Wang ◽  
...  
Keyword(s):  
Spring Wheat ◽  
Pollen Germination ◽  
Pollen Viability ◽  
Triticum Aestivum L ◽  
In Vitro Pollen Germination ◽  
Low Temperature Storage ◽  
Fresh Pollen ◽  
Storage Temperatures ◽  
Temperature Storage

The study was conducted to evaluate in vitro pollen germination of 50 spring wheat (Triticum aestivum L.) accessions at three storage temperatures (23 °C, 5 °C, and -20 °C). Germination of the mature pollen was measured right after harvest (0 time), and 24, 48, and 72 h after storage (HAS). Differences in fresh pollen germination between accessions were significant and ranged from 21.15% to 1.09%. Pollen germination was the lowest at 24 h at room temperature. The pollens of 24 wheat accessions (AAC Scotia, AW636, AW725, AW780, AW804, AW822, AW823, Brookfield, BRS Pardela, ECSW05, ECSW38, ECSW48, ECSW49, ECSW69, Hoffman, Kleos, Major, Nass, 12NQW-237, 12NQW-413, 12NQW-414, 12NQW-436, 12NQW-754, and 12BW0374) were viable after 24 h when they were stored at 5 °C. Sub-zero temperature did not prolong pollen viability.

scholarly journals Low-temperature Storage of Garlic for Spring Planting

HortScience ◽  
2004 ◽  
Vol 39 (3) ◽  
pp. 571-573 ◽  
Author(s):  
Gayle M. Volk ◽  
Kate E. Rotindo ◽  
Walter Lyons
Keyword(s):  
Room Temperature ◽  
Allium Sativum ◽  
Shoot Elongation ◽  
Storage Conditions ◽  
Low Temperature Storage ◽  
C Storage ◽  
Time Of Harvest ◽  
Spring Planting ◽  
Temperature Storage

Garlic bulbs (Allium sativum L.) harvested in the summer are often stored at room temperature between the time of harvest and curing and either consumption or planting in the fall. The quality of these bulbs usually deteriorates dramatically by 6 months after harvest. Garlic bulbs were placed at -3, 0, or 5 °C for ≈6 months to determine if bulbs could be maintained for spring planting. Response to cold-storage conditions was cultivar dependent. We found that most cured garlic bulbs stored at -3 °C for 6 months successfully formed cloves within bulbs when planted in the following spring. Unlike the high-quality bulbs formed after -3 °C storage, bulbs stored at 0 °C for 6 months often formed side cloves and had loose wrappers. In another study, garlic bulbs stored at 0, 5, 15, or 23 °C exhibited a higher rate of shoot elongation within the cloves during storage than bulbs stored at -3 °C. After 9 months of -3 °C storage, bulbs then held at room temperature retained the quality characteristics of freshly harvested garlic (firmness, taste) for at least 2 months. These studies suggest that cured garlic can be spring planted and consumed year-round when bulbs are stored at -3 °C.

scholarly journals Multi-year analyses on three populations reveal the first stable QTLs for tolerance to rain-induced fruit cracking in sweet cherry (Prunus avium L.)

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
José Quero-García ◽  
Philippe Letourmy ◽  
José Antonio Campoy ◽  
Camille Branchereau ◽  
Svetoslav Malchev ◽  
...  
Keyword(s):  
Confidence Intervals ◽  
Sweet Cherry ◽  
Prunus Avium ◽  
Linear Models ◽  
Basic Research ◽  
Fruit Weight ◽  
Phenotypic Variance ◽  
Fruit Cracking ◽  
The Stability ◽  
Sweet Cherry Cultivars

AbstractRain-induced fruit cracking is a major problem in sweet cherry cultivation. Basic research has been conducted to disentangle the physiological and mechanistic bases of this complex phenomenon, whereas genetic studies have lagged behind. The objective of this work was to disentangle the genetic determinism of rain-induced fruit cracking. We hypothesized that a large genetic variation would be revealed, by visual field observations conducted on mapping populations derived from well-contrasted cultivars for cracking tolerance. Three populations were evaluated over 7–8 years by estimating the proportion of cracked fruits for each genotype at maturity, at three different areas of the sweet cherry fruit: pistillar end, stem end, and fruit side. An original approach was adopted to integrate, within simple linear models, covariates potentially related to cracking, such as rainfall accumulation before harvest, fruit weight, and firmness. We found the first stable quantitative trait loci (QTLs) for cherry fruit cracking, explaining percentages of phenotypic variance above 20%, for each of these three types of cracking tolerance, in different linkage groups, confirming the high complexity of this trait. For these and other QTLs, further analyses suggested the existence of at least two-linked QTLs in each linkage group, some of which showed confidence intervals close to 5 cM. These promising results open the possibility of developing marker-assisted selection strategies to select cracking-tolerant sweet cherry cultivars. Further studies are needed to confirm the stability of the reported QTLs over different genetic backgrounds and environments and to narrow down the QTL confidence intervals, allowing the exploration of underlying candidate genes.

scholarly journals Viability of Najawa carp (Cyprinus carpio L.) sperm at 4°C temperature storage

2020 ◽  
Vol 147 ◽  
pp. 01015
Author(s):  
Dimas Fendy Pradana ◽  
Ignatius Hardaningsih ◽  
Dini Wahyu Kartika Sari
Keyword(s):  
Low Temperature ◽  
Cyprinus Carpio ◽  
Sperm Viability ◽  
Low Temperature Storage ◽  
Progressive Motility ◽  
C Storage ◽  
Randomized Design ◽  
Significant Difference ◽  
Carp Cyprinus Carpio ◽  
Temperature Storage

The objectives of this study were to evaluate the sperm viability of Najawa carp (Cyprinus carpio L.) in cryopreservation pre-conditions at 4°C. The design used in this study was Complete Randomized Design with 4 treatments, BSS as a control, 10% DMSO, 0,2 M Sucrose, and 5% DMSO + 0,1 M Sucrose; each consist of three replications. The parameters observed were progressive motility of fresh sperm, diluted sperm before low temperature storage, and 2 hours; 3 hours; 4 hours; 5 hours; one day; one week; a month after 4°C storage. The data were analyzed by ANOVA. The data showed that there was no significant difference between treatment (P>0.05). The best viability was 40.56% of sperm motility which survive for one week, it was achieved by 5% DMSO + 0,1 M Sucrose.

scholarly journals Self-incompatibility allele identification in Ukrainian sweet cherry (Prunus avium L.) cultivars

2018 ◽  
Vol 15 (2) ◽  
pp. 150-158
Author(s):  
Ya. I. Ivanovych ◽  
N. V. Tryapitsyna ◽  
K. M. Udovychenko ◽  
R. A. Volkov
Keyword(s):  
Sweet Cherry ◽  
Prunus Avium ◽  
Electrophoretic Analysis ◽  
Self Incompatibility ◽  
Incompatibility Group ◽  
S Alleles ◽  
Pcr Products ◽  
Incompatibility Alleles ◽  
Sweet Cherry Cultivars ◽  
Consensus Primers

Aim. Ukrainian breeders have created a large number of sweet cherry cultivars, which still remain almost unexplored at the molecular level. The aim of our study was to identify the self-incompatibility alleles (S-alleles) in Ukrainian sweet cherry cultivars and landraces, and to elucidate, to which cross-incompatibility group the cultivars belong. Methods. The PCR was conducted using consensus primers to the first and second introns of S-RNAse gene and to the single intron of SFB gene. The electrophoretic analysis of the PCR products of the second intron of S-RNAse was carried out in agarose gel, whereas detection of fluorescently labeled DNA fragments of the first S-RNAse intron and the SFB intron was performed using a genetic analyzer. Results. The S-alleles of 25 Ukrainian sweet cherry cultivars and 10 landraces were identified. The S-alleles frequencies and affiliation of cultivars and landraces to the groups of cross-incompatibility were determined. The obtained data can be used in breeding programs and by planning of industrial plantings. Conclusions. In the study, 12 different S-alleles and 79 S-haplotypes were identified. The S1, S3, S4, S5, S6 and S9 alleles are the most widespread among Ukrainian sweet cherry cultivars and landraces. The high frequencies of S5 and especially of S9 alleles are characteristic for the Ukrainian cultivars and distinguish them from other European ones. For the Ukrainian sweet cherry cultivars, the XXXVII (S5S9) cross-incompatibility group appeared to be the most numerous.Keywords: Ukrainian sweet cherry cultivars, S-locus, Sgenotypes, self- and cross-incompatibility, Prunus avium.

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