scholarly journals Sunflower Seed Hulls as Substrate for the Cultivation of Shiitake Mushrooms

2002 ◽  
Vol 12 (4) ◽  
pp. 652-655 ◽  
Author(s):  
Néstor Curvetto ◽  
Débora Figlas ◽  
Silvia Delmastro

Nutritive agar formulations with additions of poplar (Populus alba) sawdust, wheat (Triticum durum) bran, or milled sunflower (Helianthus annuus) seed hulls (SSH) were evaluated for mycelium cultivation of shiitake (Lentinula edodes), in petri dishes. Sawdust, 2, 3 and 4 g·L-1 (0.27, 0.40 and 0.53 oz/gal) added to MYA (malt, yeast extract and agar) medium did not improve the mycelium growth rate, while media that included 1, 2, and 3 g·L-1 (0.13, 0.27, and 0.40 oz/gal) wheat bran or 2, 3, and 4 g·L-1 (0.27, 0.40, and 0.53 oz/gal) milled SSH exhibited a significant increase in the mycelium growth rate, at 25 °C (77 °F). The use of SSH obtained directly from the oil industry was evaluated as a substrate for the cultivation of shiitake mushroom via synthetic logs in plastic bags. A linear growth test was used to previously assay the mycelium growth rate in substrate compositions with different contents of SSH, wheat bran, and poplar sawdust, at 25 °C. The largest mycelial growth rates were 2.75, 2.88, and 2.93 mm·d-1 (0.108, 0.113, and 0.115 inch/day) for the substrates formulated with 8 SSH: 2 wheat bran, 9 SSH: 1 poplar sawdust, and 8 SSH: 1 wheat bran: 1 poplar sawdust by weight, respectively. The synthetic logs showed a daily production rate of 2 kg shiitake/100 kg dry substrate for a 55 days cycle production with a simple formula containing 37.5% SSH, 0.5% calcium carbonate (CaCO3), 2% calcium sulfate (CaSO4), and 60% water. Addition of wheat bran to the SSH-based synthetic log produced no significant differences on biological efficiency, mushroom production, or productivity.

Foods ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 239 ◽  
Author(s):  
Aikaterini Papadaki ◽  
Panagiota Diamantopoulou ◽  
Seraphim Papanikolaou ◽  
Antonios Philippoussis

Morchella sp. is one of the most expensive mushrooms with a high nutritional profile. In this study, the polysaccharide content of Morchella species was investigated. Specifically, mycelium growth rate, biomass production, sclerotia formation, and glucosamine and total polysaccharides content of six Morchella species grown on a starch-based media were evaluated. Submerged fermentations in potato dextrose broth resulted in a glucosamine content of around 3.0%. In solid-state fermentations (SSF), using potato dextrose agar, a high linear growth rate (20.6 mm/day) was determined. Increased glucosamine and total polysaccharides content were observed after the formation of sclerotia. Biomass and glucosamine content were correlated, and the equations were used for the indirect estimation of biomass in SSF with agro-industrial starch-based materials. Wheat grains (WG), potato peels (PP), and a mixture of 1:1 of them (WG–PP) were evaluated as substrates. Results showed that the highest growth rate of 9.05 mm/day was determined on WG and the maximum biomass yield (407 mg/g) on WG–PP. The total polysaccharide content reached up to 18.4% of dried biomass in WG–PP. The results of the present study proved encouraging for the efficient bioconversion of potato and other starch-based agro-industrial waste streams to morel biomass and sclerotia eliciting nutritional and bioactive value.


2021 ◽  
Vol 36 (1) ◽  
pp. 1-13
Author(s):  
Ana Obradovic ◽  
Jelena Vukadinovic ◽  
Milica Nikolic ◽  
Aleksandra Bulajic ◽  
Goran Stankovic ◽  
...  

The aim of this study was to assess variations in aggressiveness and trichothecene production of F. graminearum isolates originating from maize, wheat and barley in Serbia. Analyzing F. graminearum isolates (98) obtained from various agroecological conditions of Serbia over the period from 1993 to 2010, using the HPLC method, the following two chemotypes were observed: 3-acetyl-deoxinivalenol (3ADON) and 15-acetyl-deoxinivalenol (15ADON). A great diversity in the production of deoxinivalenol (DON) derivatives was observed. A majority of F. graminearum isolates, regardless of their origin (maize, wheat or barley) belonged to the 15ADON chemotype. The 3ADON chemotype was also detected, but in a significantly smaller number (13/98) samples, compared to the 15ADON chemotype (85/98). None of the tested isolates belonged to the NIV chemotype. The examined isolates showed different pathogenicity on barley leaf, wheat class and maize ears. The average pathogenicity of the tested isolates was the highest on barley leaf. It was observed that isolates originating from wheat had the highest average daily increase in mycelium growth rate (27.37 mm). Statistical analysis of the obtained results for mycotoxins synthesis showed that there was a highly significant statistical correlation between the production potentials of total DON, 3ADON and 15ADON in F. graminearum isolates belonging to various chemotypes. However, there was no statistically significant correlation between the aggressiveness of isolates and the production of total DON in isolates belonging to 3ADON and 15ADON chemotypes.


2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Ying-Fan Liu ◽  
Lei Wang ◽  
Chun-Po Bu ◽  
Guo-Qing Wang ◽  
Yan-Hui Zhang ◽  
...  

This paper focuses on the synthesis of novel Ag nanoclusters (NCs) using DHLA as capping reagents in aqueous solution by a photoreduction method. Luminescence studies indicated that the DHLA-Ag NCs exhibited strong blue emission with maximum peak at 480 nm. The maximum emission of the NCs can be greatly improved with irradiating time by around 15-fold from 3 h to 67 h. By means of mycelium growth rate, the results showed that the Ag NCs with smaller sizes had a good antimicrobial effect.


2013 ◽  
Vol 38 (2) ◽  
pp. 137-145
Author(s):  
Hanna Bryk

Variability in size of conidia, formation of sclerotia and mycelial growth were examined in monoconidial cultures of 5 isolates of <i>Botrytis cinerea</i> Pers. The influence of two media on the above-mentioned features was also studied. It was found that <i>Botrytis cinerea</i> Pers. is relatively homogenous in respect to the size of conidia. Out of the 50 monoconidial cultures tested, only two had conidia somewhat larger than the remaining cultures. Monoconidial cultures derived from the same isolate varied among themselves in the rate of mycelial growth and in the number and size of sclerotia. The kind of medium used influenced mycelial growth and sclerotia formation. Better mycelial growth was observed on PDA medium than on Czapek's medium. On the latter, the fungus produced more sclerotia which were, however, smaller in size than on PDA medium.


2017 ◽  
Vol 109 (3) ◽  
pp. 555 ◽  
Author(s):  
Mohammad Ebrahim RANJBAR ◽  
Jamal Ali OLFATI ◽  
Masoumeh AMANI

<p><em></em>Shiitake is an edible mushroom native to East Asia. In the present research, the soaking water was targeted as the vehicle to enrich the substrate. The amount of nutrients in the substrate is severely reduced by mycelium growth and development during spawn running and browning period. Some part of this reduction can be compensated by soaking the substrate in the enriched soaking water. In this study, soaking water was enriched by some complement materials and enrichment effects on some important properties of shiitake mushroom were evaluated. The highest biological efficiency (69.88 %) was gained with soaking the blocks in wheat bran extraction suspension. The highest dry matter of mushroom was obtained by rice bran extraction suspension as the enriched soaking water. The results of this research showed that some important properties of shiitake mushroom can be improved by soaking the blocks with enriched soaking water. According to the results, wheat bran extraction suspension was the best enriched solution to increase productivity of shiitake mushrooms and rice bran extraction suspension was suitable to improve quality of mushrooms.<strong></strong></p>


2021 ◽  
Vol 911 (1) ◽  
pp. 012044
Author(s):  
R Muslimin ◽  
H Hartono ◽  
R Rachmawaty ◽  
A Ali ◽  
M Junda ◽  
...  

Abstract The study aimed to find alternative substrates for growing spawn of oyster mushroom (Pleurotus ostreatus). The experiment was laid out in a completely randomized design with three compositions of substrates. The substrate compositions were: (1) Substrate A (corn 100%), (2) Substrate B (corn and sawdust mix in a ratio of (1:1, v/v)), and (3) Substrate C (corn and sawdust mix in a ratio of (3:1, v/v)). All compositions were replicated 18 times. The complete colonization (days) and mycelium growth rate (cm day−1) were recorded. Means were analyzed by Analysis of Variance (ANOVA) and duncan test was performed if there were significant between the substrate compositions. The results showed that substrate C was the best substrate indicated with the fastest complete colonization of 14.17±0.92 and the highest growth rate of 0.85±0.06 cm day−1.


2017 ◽  
Vol 48 (4) ◽  
Author(s):  
Abdulrazzaq & et al.

This study was conducted to evaluate the efficiency of the substrate on the productivity and quality for Flammulina velutipes. Results of the effect of different agro-waste sawdust (SD), wheat straw (WS) and palm waste (PW). on the mean fungal mycelium growth shows that Flammulina velutipes gave the highest mean fungal mycelium growth on SD culture media amended with 2, 3, 4% lime at 30c. evaluation the efficiency of the above  agro-waste  substrate morphological and productivity showed that, the shortest period for the full growth of F. velutipes mycelium was on SD substrate, the shortest period for the pinhead formation 8.6days on  the fungus gave highest yield on WS which was 152.3 gm/ bag with 61.1% biological efficiency. The shortest time for full growth of F. velutipes was achieved in PW amended with wheat bran, the highest yield was in WS enriched with wheat bran which 306.7 gm/ bag with 129.3% biological efficiency. Results showed differences in quality characteristics in fruit bodies  of fungi by effect of substrate. 


2013 ◽  
Vol 8 (7) ◽  
pp. 701-711 ◽  
Author(s):  
Lidia Irzykowska ◽  
Jan Bocianowski ◽  
Anna Baturo-Cieśniewska

Abstract


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