The effect of different substrates on oyster mushroom (Pleurotus ostreatus) spawn growth

Abstract The study aimed to find alternative substrates for growing spawn of oyster mushroom (Pleurotus ostreatus). The experiment was laid out in a completely randomized design with three compositions of substrates. The substrate compositions were: (1) Substrate A (corn 100%), (2) Substrate B (corn and sawdust mix in a ratio of (1:1, v/v)), and (3) Substrate C (corn and sawdust mix in a ratio of (3:1, v/v)). All compositions were replicated 18 times. The complete colonization (days) and mycelium growth rate (cm day−1) were recorded. Means were analyzed by Analysis of Variance (ANOVA) and duncan test was performed if there were significant between the substrate compositions. The results showed that substrate C was the best substrate indicated with the fastest complete colonization of 14.17±0.92 and the highest growth rate of 0.85±0.06 cm day−1.

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Pengaruh penambahan serbuk jagung pada komposisi media tanam terhadap pertumbuhan miselium bibit F1 jamur tiram putih (Pleurotus ostreatus)

BIOMA Jurnal Ilmiah Biologi ◽

10.26877/bioma.v10i2.7429 ◽

2021 ◽

Vol 10 (2) ◽

pp. 158-170

Author(s):

Muhaeming Muhaeming ◽

Jamilah Jamilah ◽

Zulkarnaim Zulkarnaim

Keyword(s):

Experimental Study ◽

Analysis Of Variance ◽

Pleurotus Ostreatus ◽

Medium Composition ◽

Oyster Mushroom ◽

Control Treatment ◽

Mycelium Growth ◽

Randomized Design ◽

Significant Difference ◽

Completely Randomized Design

Penambahan nutrisi perlu dilakukan untuk meningkatkan produktivitas pertumbuhan jamur tiram putih (Pleurotus ostreatus). Salah satu bahan yang dapat digunakan adalah serbuk jagung. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan serbuk jagung pada komposisi media tanam terhadap pertumbuhan miselium bibit F1 jamur tiram putih (Pleurotus ostreatus) dan mengetahui konsentrasi serbuk jagung yang paling baik digunakan pada pertumbuhan miselium bibit F1 jamur tiram putih (Pleurotus ostreatus). Penelitian ini mengunakan Rancangan Acak Lengkap (RAL) 4 perlakuan dan 3 ulangan sehingga diperoleh 12 satuan percobaan; P0 (perlakuan kontrol), P1 (25%), P2 (50%), dan P3 (100%). Variabel yang diukur adalah persentase pertumbuhan miselium yang diamati pada hari ke-6, 12 dan 18 dan waktu penyebaran miselium Hari Setelah Inokulasi (HSI). Data hasil penelitian dianalisis menggunakan analysis of variance (ANOVA) dengan uji lanjut menggunakan uji Beda Nyata Terkecil (BNT). Hasil penelitian menunjukkan bahwa ada pengaruh pada pertumbuhan miselium bibit F1 jamur tiram putih (Pleurotus ostreatus) dengan penambahan serbuk jagung pada komposisi media tanam. Selain Itu, konsentrasi serbuk jagung yang memberikan hasil yang paling baik terhadap pertumbuhan miselium adalah P2 dengan konsentrasi 50%. Kata kunci : miselium F1; Pleurotus ostreatus; serbuk jagung ABSTRACTAdditional nutrition needs to be done to increase the productivity of oyster mushroom (Pleurotus ostreatus) growth. One of the materials that can be used is corn powder. This study aimed to determine the effect of corn powder on the planting medium composition towards mycelium growth of oyster mushroom F1 seeds (Pleurotus ostreatus) and to find the best concentration of corn powder to grow mycelium seeds F1 oyster mushroom (Pleurotus ostreatus). This research was an experimental study using a Completely Randomized Design (CRD) with 4 treatments namely; P0 (control treatment), P1 (25%), P2 (50%), and P3 (100%), and 3 repetitions so that obtained 12 experimental units. The variables measured were the mycelium growth percentage on days 6, 12, and 18, and the mycelium widespread time after the day of inoculation. The data were analyzed using ANOVA then continued on the Least Significant Difference (LSD) test. The results showed that the addition of corn powder on oyster mushroom growing media affected mycelium growth of the oyster mushroom F1 seeds (Pleurotus ostreatus). It also found that the P2 with a 50% concentration of corn powder gave the widest mycelium growth. Keywords: corn powder; mycelium; Pleurotus ostreatus

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Efek Penambahan Limbah Lokal Jerami Dan Sekam Padi Bagi Pertumbuhan Jamur Tiram Putih (Pleurotus ostreatus)

Bioeksperimen Jurnal Penelitian Biologi ◽

10.23917/bioeksperimen.v5i2.9233 ◽

2019 ◽

Vol 5 (2) ◽

pp. 70-76

Author(s):

Afifah Nur Shobah ◽

Swastika Oktavia

Keyword(s):

Growth Rate ◽

Pleurotus Ostreatus ◽

Dry Weight ◽

Oyster Mushroom ◽

Microscopic Fungi ◽

Artificial Medium ◽

Mycelium Growth ◽

Randomized Design ◽

Wet Weight ◽

Completely Randomized Design

White oyster mushroom (Pleurotus ostreatus) is a group of microscopic fungi that are used as food. P. ostreatus is cultivated in an artificial medium derived from sawdust and has been sterilized. However, the use of sawdust also has problems. Straw and husk of rice can be used as mushroom growing media because they contain organic ingredients such as cellulose, hemicellulose, and lignin. This study aimed to know the growth of white oyster mushroom (P. ostreatus) on straw and husk of rice as an artificial medium and to know the best composition of straw and husk of rice that can be got highly produced of white oyster mushroom (P. ostreatus). The methods of this research were experimental with ten treatments and included several stages including preparation of tools, materials and research sites, the stage of cultivation of P. ostreatus and data collection. This study used an experimental method with a completely randomized design consisting of 40 experimental units. The results obtained were the most optimal P. ostreatus mycelium growth in K1J2S1 treatment with a mean growth rate of 30,60 cm / 30 days, the highest wet weight was K3J1S0 which was 85,83 g while the highest dry weight was in K1J2S1 treatment that is equal to 8,71 g.

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Pemanfaatan Limbah Serbuk Teh sebagai Substitusi Serbuk Gergaji terhadap Pertumbuhan Miselium dan Produksi Jamur Tiram Putih (Pleurotus Ostreatus)

Agrotekma Jurnal Agroteknologi dan Ilmu Pertanian ◽

10.31289/agr.v3i1.2065 ◽

2018 ◽

Vol 3 (1) ◽

pp. 27

Author(s):

Siti Mardiana ◽

Ellen L Panggabean ◽

Retno A Kuswardani ◽

Muhammad Usman

Keyword(s):

Fruiting Body ◽

Pleurotus Ostreatus ◽

Fruit Body ◽

Oyster Mushroom ◽

Combined Treatments ◽

Mycelium Growth ◽

Growing Media ◽

Randomized Design ◽

Tea Waste ◽

Completely Randomized Design

<p class="HowToCite"><em>The waste of tea powder is abundant and has not been widely used optimally. The alternative use is to use the waste as an additional medium or substitution for the white oyster mushroom growing media. The purpose of the study was to determine the effect of mycelium growth and the production of the fruit of the white oyster mushroom on combination media / tea powder substitution with sawdust. The research design used was a completely randomized design (CRD) with 4 replications. Combined treatments were M0 = 100% sawdust; M1 = 100% Tea waste powder; M2 = 75% tea powder + 25% sawdust; M3 = 50% tea powder + 50% sawdust and M4 = 25% tea powder + 75% sawdust. All treatments used the composition of the manufacture of mushroom growing media consisting of each combination of treatments, bran 10% by weight of the material and lime 0.5% by weight of the material. The parameters observed were the growth of white oyster mushroom mycelium, the rate of mycelium growth, the time the mycelium closed the substrate, the time of appearance of the fruiting body, the weight of the fruit body and the diameter of the fruit hood. The results showed that all parameters differed significantly. The best treatment is in treatment M4, followed by treatment of M3, M0 and M2.</em></p>

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Potential Of Various Types of Media for Breeding Oyster Mushroom F2

E3S Web of Conferences ◽

10.1051/e3sconf/202132808015 ◽

2021 ◽

Vol 328 ◽

pp. 08015

Author(s):

Widiwurjani ◽

Ida Retno Mulyani ◽

Ilmatus Sa’diyah ◽

N.K. Sari

Keyword(s):

Growth Rate ◽

Rice Bran ◽

Oyster Mushroom ◽

Growth Speed ◽

Soybean Seeds ◽

Mycelium Growth ◽

Randomized Design ◽

Mycelium Growth Rate ◽

Materials Used ◽

Corn Kernels

This research was conducted at the Laboratory of Biotechnology 1 and Farmers' Land in Karanganyar village, Poncokusumo, Malang. The materials used in this study were white oyster mushroom F1 seeds, 70% alcohol, soybean seeds, corn seeds, rice, sawdust, rice bran and mushroom baglog. This study used an experimental design, namely Completely Randomized Design (CRD) with 8 treatment levels B1 = corn kernels (100%), B2 = soybean seeds (100%), B3 = rice (100%), B4 = sawdust (100%), B5 = corn kernels (50%) + sawdust (40%) + rice bran (10%). Parameters observed were height of Mycelium-covered Media (cm), Mycelium Growth Speed (cm/day), Contamination Occurrence Percentage (%). The average yield of the highest seedling height was 12.79 cm in treatment B8. The parameter of mycelium growth rate in B8 treatment had the fastest growth rate of 1.82 cm/ha. The lowest contamination was found in treatment B5, B6 by 4% and B7 by 20%. Treatment B3, B4 and B8 did not experience contamination.

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Growth performance and blood profile of rabbit bucks in two housing types on aqueous extract of oyster mushroom (Pleurotus ostreatus Jacq ex fr.)

Nigerian Journal of Animal Production ◽

10.51791/njap.v46i1.1316 ◽

2020 ◽

Vol 46 (1) ◽

pp. 51-60

Author(s):

O. M. Sogunle ◽

T. S. Aremu ◽

O. O. Olatubosun ◽

C. P. Njoku ◽

O. M. Sogunle ◽

...

Keyword(s):

Growth Performance ◽

Pleurotus Ostreatus ◽

Octadecadienoic Acid ◽

Conversion Ratio ◽

Oyster Mushroom ◽

Feed Conversion ◽

Blood Profile ◽

Mushroom Extract ◽

Randomized Design ◽

Completely Randomized Design

The growth performance and blood profile of forty-eight (48), six (6) weeks old rabbit bucks of cross (Chinchilla and New Zealand White) were assessed for 10 weeks. The bucks were allotted on weight equalization basis in a 2 x 4 factorial experimental layout composed of 24 rabbits into steel hutches and wooden hutches each and on four (4) varying levels (0, 5, 10 and 15 mL/litre of water, respectively) of oyster mushroom (Pleurotus ostreatus Jacq ex fr.) extract. Six (6) bucks were assigned to each treatment group and replicated three times with two (2) bucks per replicate in a Completely Randomized Design. The phytochemicals of the oyster mushroom extract showed that 9,12-Octadecadienoic acid (Z, Z)- methyl ester was the most abundant. Results showed that the feed conversion ratio was significantly (P<0.05) best (6.08) in bucks reared in wooden cage and administered 15 mL oyster mushroom extract. Significantly (P<0.05) highest triglycerides (81.00 mg/dL) was obtained in bucks on steel hutch and on 5 mL oyster mushroom extract and lowest (55.00 mg/dL) in bucks on steel hutch and on 10 mL oyster mushroom extract. The lowest (P<0.05) lymphocytes (59.00%) were recorded in bucks on wooden cage and administered 10 mL oyster mushroom extract. It was concluded that oyster mushroom extract up to 15 mL/litre of water administered in either steel hutch or wooden hutch did not impair the growth performance and blood profile of rabbit bucks.

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Pengaruh Pemberian Probiotik Berbeda Pada Pakan Komersial Terhadap Pertumbuhan Dan Efisiensi Pakan Ikan Lele Sangkuriang (Clarias Sp.) [ The Present Effect Of Different Probiotics On Commercial Feed Towards Growth And Feed Efficiency Of Sangkuriang Catfish (Clarias Sp.) ]

Jurnal Ilmiah Perikanan dan Kelautan ◽

10.20473/jipk.v6i1.11381 ◽

2019 ◽

Vol 6 (1) ◽

pp. 49

Author(s):

Muhammad Arief, Nur Fitriani, Sri Subekti

Keyword(s):

Growth Rate ◽

Experimental Method ◽

Analysis Of Variance ◽

Feed Efficiency ◽

Duncan Multiple Range Test ◽

Randomized Design ◽

Commercial Feed ◽

Completely Randomized Design ◽

Range Test ◽

Significant Data

Abstract Freshwater culture commodity such as sangkuriang catfish (Clarias sp.) has high sufficient demand. One way to fullfil necessity of sangkuriang catfis demand is accelerate the growth. The aim of this research is to determine the present effect of different probiotics on commercial feed towards growth and feed efficiency of sangkuriang catfish. The research using experimental method, using Completely Randomized Design (CRD). Sangkuriang catfish kept in 30 days with four treatments and five replications, that is P1 (control), P2 (5% probiotics A), P3 (5% probiotics B), and P4 (5% probiotics C). The obtained data were processed by Analysis of Variance (ANOVA) and followed by Duncan Multiple Range Test if there was significant data. The result showed that different probiotics giving significant effect to growth rate and feed efficiency (p<0,05). The best sangkuriang catfish (Clarias sp.) growth rate is P2 treatment 2,88% ± 0,38, and the lowest is P1 treatment at 1,73% ± 0,7. The best feed efficiency is P2 treatment at 54,69% ± 9,67 and the lowest is P1 treatment at 30,27% ± 12,65

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Yield performance of oyster mushroom (Pleurotus ostreatus) on different substrates

Bangladesh Journal of Agricultural Research ◽

10.3329/bjar.v38i4.18946 ◽

2014 ◽

Vol 38 (4) ◽

pp. 613-623 ◽

Cited By ~ 1

Author(s):

D Das ◽

M Kadiruzzaman ◽

SK Adhikary ◽

MY Kabir ◽

M Akhtaruzzaman

Keyword(s):

Sugarcane Bagasse ◽

Pleurotus Ostreatus ◽

Growth And Yield ◽

Oyster Mushroom ◽

Fruiting Bodies ◽

Yield Attributes ◽

Randomized Design ◽

Biological Yield ◽

Completely Randomized Design ◽

Coconut Coir

An experiment was conducted at Mushroom Lab of Horticulture Centre under Department of Agricultural Extension (DAE), Khairtala, Jessore to determine the performance of different substrates on the yield of oyster mushroom (Pleurotus ostreatus). Sugarcane bagasse, sawdust, and coconut coir individually and their combinations were used as substrates. The experiment was laid out in Completely Randomized Design (CRD) with five replications. Data were taken from 1st and 2nd flush of different growth and yield attributes of mushroom. The maximum number of effective fruiting bodies was obtained from sawdust (40) and the lowest number (31.6) from combination of sawdust and coconut coir (1:1). In the 1st flush, the maximum weight of individual fruiting body was observed in coconut coir and minimum in sawdust. The highest biological (186.06 g) and economic yield (180.64 g) were obtained from coconut coir and the lowest from sugarcane bagasse. The maximum and minimum harvest index was found in 1st flush at coconut coir and combination of sawdust and sugarcane bagasse (1:1), respectively. Most yield attributes were found higher in coconut coir. Economic yield was positively correlated to number of effective fruiting bodies, pileus diameter, and biological yield. DOI: http://dx.doi.org/10.3329/bjar.v38i4.18946 Bangladesh J. Agril. Res. 38(4): 613-623, December 2013

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The Growth Of White Oyster Mushroom Mycelium (Pleurotus ostreatus) (Jacq) (P. Kumm) From Liquid And Solid Inoculum

Biocelebes ◽

10.22487/bioceb.v13i3.14971 ◽

2019 ◽

Vol 13 (3) ◽

pp. 279-287

Author(s):

Nisfaun Safitriana ◽

Umrah ◽

Orryani Lambui

Keyword(s):

Liquid Medium ◽

Incubation Time ◽

Pleurotus Ostreatus ◽

Solid Medium ◽

Oyster Mushroom ◽

Mycelium Growth ◽

Average Growth ◽

Solid Media ◽

Randomized Design ◽

Department Faculty

The study about the growth of white oyster mushroom mycelium (Pleurotus ostreatus) (Jacq) (P. Kumm) from liquid and solid inoculum conducted on January until July 2018 in Biotechnology laboratory, Biology Department, Faculty of Math and Science, Tadulako University. This study was aimed to find out the mycelium growth of source of stem inoculum and hood in liquid and solid media. This study was designed by Complete Randomized Design (CRD) which consisted of four treatments and five replications. The treatment were M1 (source of stem inoculum in liquid medium), M2 (source of stem inoculum in solid medium), M3 (source of hood inoculum in liquid medium), and M4 (source of hood inoculum in solid medium). The parameters were (a) incubation time till mycelium fills medium, (b) CFU, and (c) the viability of inoculum on producing medium. The result showed that the faster incubation were M1 and M3 (for 2 days, higher CFU was M3 with an average number 8,2 x 10 10 CFU /ml. The faster viability of mycelium growth were M3 with an average growth of mycelium 6,97 cm/days.

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Optimization of growth of oyster mushroom mycelium (Pleurotus sp.) from Tasikmalaya on several kinds of cereal medium

Journal of Microbial Systematics and Biotechnology ◽

10.37604/jmsb.v1i2.27 ◽

2019 ◽

Vol 1 (2) ◽

pp. 13-17

Author(s):

Khusnul Khusnul

Keyword(s):

Human Body ◽

Edible Mushroom ◽

Oyster Mushroom ◽

Mushroom Cultivation ◽

Mycelium Growth ◽

Randomized Design ◽

Growing Medium ◽

Completely Randomized Design ◽

Seed Medium

Pleurotus sp. or oyster mushroom is one type of edible mushroom that contains essential nutrients for the human body. This mushroom has been widely cultivated in Indonesia, one of which is Pleurotus sp. from Tasikmalaya. The key to the success of oyster mushroom cultivation is the seeds and growing medium or baglog, while the factors that influence the quality of the seeds include the type of medium. This study aims to determine 1) the effect of the type of seed medium from the type of cereals used on the growth of mycelium Pleurotus sp. from Tasikmalaya, 2) determine the type of seed medium that has the best mycelium growth. This study uses an experimental method with a Completely Randomized Design (CRD). The parameters measured include the main parameters, namely the rate of growth of mycelium Pleurotus sp. from Tasikmalaya on the seed medium. Supporting parameters are the quality of mycelium growth in the seed medium, the proximate value of cereals used for the seed medium. The results of the analysis showed that the medium of cereals used as seeds ready for cultivation could affect the quality of the seeds produced. The seedling medium from milled corn has the best growth of mycelium.

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Pertumbuhan rumput laut Kappaphycus alvarezii hasil kultur jaringan pada jarak tanam yang berbeda

DEPIK ◽

10.13170/depik.5.1.3843 ◽

2016 ◽

Vol 5 (1) ◽

Author(s):

Ayuningsih Ria Sapitri ◽

Nunik Cokrowati ◽

Rusman .

Keyword(s):

Growth Rate ◽

Tissue Culture ◽

Weight Gain ◽

Analysis Of Variance ◽

Kappaphycus Alvarezii ◽

Initial Weight ◽

Microsoft Excel ◽

Randomized Design ◽

Significant Difference ◽

Completely Randomized Design

Abstract. The purpose of this study is to determine the best planting space on the growth of regenerated tissue culture Kappaphycusalvarezii. The completely randomized design (CRD) was utilized in this study, thre treatment was four different planting spaces; P1: 15cm, P2: 20 cm, P3: 25 cm, and P4: 30 cm with initial weight of 100g/hill. Every treatment has four replicates (four rafts). The data were subjected to one way of analysis of variance (ANOVA) at 5% of error levels and followed by Least Significant Difference (LSD) test. The Anova test showed that planting space gave a significant affect on weight gain and growth rate where the best planting space was 25 cm with weight gain and growth rate of 331.4 g and 4.87% perday, respectively. Keywords: K.alvarezii;space; tissue culture regenerated;growth;photosynthesis Abstrak. Tujuan penelitian ini adalah untuk menentukan jarak tanam optimum untuk pertumbuhan rumput laut Kappaphycus alvarezii hasil kultur jaringan. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan menggunakan bibit hasil kultur jaringan dengan jarak tanam yang berbeda yakni P1: jarak tanam 15cm , P2: jarak tanam 20 cm, P3: jarak tanam 25 cm, dan P4: jarak tanam 30 cm dengan berat awal 100 g/rumpun, masing-masing perlakuan dengan empat kali ulangan pada 4 rakit. Data penelitian yang diperoleh, ditabulasi menggunakan Microsoft excel dan dianalisis menggunakan analisis sidik ragam (ANOVA) pada taraf kesalahan 5%, kemudian dilakukan uji lanjut dengan uji Least Significant Difference (LSD). Hasil penelitian ini menunjukkan bahwa jarak tanam berpengaruh nyata terhadap pertumbuhan rumput laut (P<0,05), dimana jarak tanam terbaik adalah 25 cm dengan pertambahan bobot 331,4 g dan laju pertumbuhan 4,87% per hari.Kata Kunci: K. alvarezii; Jarak tanam; kultur jaringan; pertumbuhan; fotosintesis

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