scholarly journals Immobilization of Mycelial Pellets from Liquid Spawn of Oyster Mushroom Based on Carrier Adsorption

2011 ◽  
Vol 21 (1) ◽  
pp. 82-86 ◽  
Author(s):  
Lanqing Wang ◽  
Yinfeng Li ◽  
Dehai Liu ◽  
Chaohui Zhang ◽  
Yuancheng Qi ◽  
...  
Keyword(s):  
Polyphenol Oxidase ◽  
Wheat Bran ◽  
Storage Time ◽  
Practical Method ◽  
Edible Mushroom ◽  
Oyster Mushroom ◽  
Respiration Intensity ◽  
Mycelial Pellets ◽  
Cottonseed Hull ◽  
Liquid Spawn

We investigated a practical method for immobilizing liquid spawn of oyster mushroom (Pleurotus ostreatus) to prolong the storage time and provide convenient transportation of liquid spawn of edible mushrooms. The method was based on the mycelial pellets of liquid spawn adsorbed in carriers. Selected carriers were similar to cultivation substrates, and the best carrier was a mixture of cottonseed hull, corn core, and wheat bran with a ratio of 4.5:4.5:1 by weight. Immobilized spawn were prepared by mixing the pellets from liquid spawn with carriers using a ratio of 1:8 by weight. Within the first 15 days of storage at 20–25 °C, the immobilized spawn grew strongly, respiration intensity and cellulase activities rose rapidly, and the count and brightness of the isozyme bands of esterase, peroxidase, and polyphenol oxidase increased remarkably as well. From days 30 to 60, the cellulase activities fell and the brightness of the peroxidase and polyphenol oxidase bands gradually decreased, whereas the respiration intensity and the band count of esterase and peroxidase remained constant. After 60 days, the cultivated characteristics of the immobilized spawn were same as the fresh conventional solid cottonseed hull spawn. The results showed that immobilized spawn on the basis of the mycelial pellets of liquid spawn adsorbed in carrier can be used to extend the storage time and simplify transportation of liquid spawn of edible mushroom.

Properties of wheat bran polyphenol oxidase

Nahrung/Food ◽  
2004 ◽  
Vol 48 (1) ◽  
pp. 5-8 ◽  
Author(s):  
Çi??dem Soysal ◽  
Zerrin Söylemez
Keyword(s):  
Polyphenol Oxidase ◽  
Wheat Bran

A New Way of Biological Treatment of Oil Residue - A Case of the Mushroom Cultivation

2011 ◽  
Vol 183-185 ◽  
pp. 411-416
Author(s):  
Xi Qin Wang ◽  
Zheng Wen Huang ◽  
Bo Yu ◽  
Jian Qiang Zhang
Keyword(s):  
Wheat Bran ◽  
Culture Medium ◽  
Raw Materials ◽  
Test Group ◽  
Raw Material ◽  
Control Group ◽  
Mushroom Cultivation ◽  
Edible Fungi ◽  
Oil Residue ◽  
Cottonseed Hull

As an industrial organic solid waste, oil residue, with wide sources and low price, but could not find an effective way to use currently. In addition, with the development of mushroom industry and rising price of traditional raw-materials, it is very necessary to find the substitute raw material to reduce production cost of mushroom cultivation. This study attempts to produce the strains of Flammulina velutipes by taking use of oil residue to replace part of the traditional raw material, there are two groups in the experiment: the test group is adding 5%, 10%, 15%, 20%, 40% of the oil residue to replace the culture medium of cottonseed hull and wheat bran; the control group is the traditional culture medium, then comparatively observed the growth of the mycelium. The results showed that it can promote the growth of the mycelium and shorten the cycle of seed production to add the oil residue in the test. But the growth rate of mycelium will be hindered when the concentration exceeds a certain range.The optimal culture medium to add the oil residue is as follows: 15% of the oil residue, 64% cottonseed hull, 20% coarse wood chips, 0% wheat bran , 1% lime. Oil residue contains a lot of the nutritional components to benefit the growth of edible fungi, and without the heavy metal pollution, can replace the culture medium of cottonseed hull and wheat bran and other traditional materials, reduce cost of production the strains, a fair-sized economic efficiency can be received, the environment can be protected.

scholarly journals Grey Oyster Mushroom Biofarm for Small-Scale Entrepreneurship

2019 ◽  
Vol 2019 ◽  
pp. 1-6
Author(s):  
Desta Berhe Sbhatu ◽  
Haftom Baraki Abraha ◽  
Hiluf Tekle Fisseha
Keyword(s):  
Wheat Bran ◽  
Oyster Mushroom ◽  
Small Scale ◽  
Optimum Conditions ◽  
Home Based ◽  
Randomized Design ◽  
Polyethylene Bags ◽  
Completely Randomized Design ◽  
Aseptic Conditions ◽  
Growing Mass

This paper reports the productivity of a small-scale pilot biofarm of grey oyster mushroom (Pleurotus sajor-caju (Fr.) Sing.). The pilot was tested in Mekelle city (Ethiopia) in a brick-walled dark room. Growing structures were constructed by erecting three wood poles and fixing them with three wooden side bars at multiple locations to make a prism-shaped rack with multiple triangular open shelves, each capable of carrying one bag of spawned substrate. Mushroom substrates were prepared from maize stalk and wheat bran supplement. Pasteurized chopped maize stalk and wheat bran were mixed at the ratio of 10:0, 9:1, 8:2, and 7:3—yielding four treatments. Five kilograms of substrate was taken from each treatment and was mixed with one kilogram of gypsum to produce a growing mass. Each mass was spawned with 200 g of inoculum under aseptic conditions and put in polyethylene bags. The treatments were replicated thrice and the bags were put on the growth racks in completely randomized design. The growing room was maintained at optimum conditions. Maize stalk substrates supplemented with 10% and 20% of wheat bran have resulted in statistically comparable productivities but statistically significantly higher than those grown on nonsupplemented and highly supplemented maize stalk substrates (p ≤ 0.05). The ingenuity of the design and the convenience of the construction of the racks, the availability of the substrates, and the simplicity of the management and maintenance of the biofarm rendered the piloted design suitable for home-based and small- and medium-scale mushroom biofarm entrepreneurship.

scholarly journals An Improved Method for Storing Yam (Dioscorea alata)

1969 ◽  
Vol 58 (4) ◽  
pp. 456-465
Author(s):  
J. R. Rivera ◽  
M. A. González ◽  
A. Collazo de Rivera ◽  
J. Cuevas-Ruiz
Keyword(s):  
Relative Humidity ◽  
Gamma Radiation ◽  
Storage Time ◽  
Practical Method ◽  
Dioscorea Alata ◽  
Improved Method ◽  
Ambient Conditions ◽  
Dormancy Period ◽  
Controlled Conditions ◽  
Significant Chemical

By storing cured yam tubers of the variety "Florido" (D. alata) at a temperature of 16° C and a relative humidity of 80 percent their dormancy period can be extended 4 additional months. No significant chemical changes are observed during the storage time. Their flavor remains unchanged and they lose very little weight. When yam tubers stored for 7 months under controlled conditions are treated with the sprout inhibitor CIPC in Dinafog form, their inactivity period is prolonged for 20 additional days. This sprout inhibitor could be used as a good and practical method to increase the transit period of the tubers if and when its use is authorized. The effects of gamma radiation in yam tubers stored for 7 months under controlled conditions were also studied. Irradiation doses of 7.5 and 10 Krad controlled their sprouting process satisfactorily. No change was observed in flavor. Treatments with 7.5 and 10 Krad doses extend the dormancy period of the tubers kept at prevailing ambient conditions for 60 additional days, thus extending the yam shelf life.

scholarly journals Can Fluted Pumpkin (Telfairia occidentalis) Hook.f. Serve as Supplement for Production of Oyster Mushroom (Pleurotus floridanus) Singer?

2020 ◽  
pp. 47-54
Author(s):  
F. Ofomana ◽  
O. M. Adedokun
Keyword(s):  
Wheat Bran ◽  
Dry Weight ◽  
Oyster Mushroom ◽  
Pumpkin Seed ◽  
Mushroom Production ◽  
Fluted Pumpkin ◽  
Telfairia Occidentalis ◽  
Significant Difference ◽  
Pleurotus Floridanus ◽  
Blocked Design

Supplement in mushroom production boosts yield. Fluted pumpkin parts were assessed as an alternative to wheat bran as supplement in mushroom production. Sawdust, lime and wheat bran or fluted pumpkin (Pod, seed, stem) were mixed in ratio 180: 1: 30. There were 10 replicates for each treatment. The design was completely randomized blocked design. The results showed that supplemented sawdust with wheat bran had higher value for the dry weight with significant difference from treatments with pumpkin parts in some flushes and none in other flushes Supplemented sawdust with mixture of all supplements was highest in Ash (14.30%) and moisture (24.75%). Protein and lipids values were highest for supplemented sawdust with wheat bran and significantly different (p<0.05) from other treatments. Sawdust supplemented with fluted pumpkin stem (28.06%) had highest value for carbohydrates while that supplemented with pod had (21.16%), the highest for fiber. For minerals, K (794.50 mg/kg), Ca (349.75 mg/kg), and Na (379.50 mg/kg) were highest for Fluted Pumpkin Seed Supplement, Fluted Pumpkin Pod Supplement and No Supplement respectively. In conclusion, Fluted pumpkin pod and stem compares very well with wheat bran supplement, and can serve as supplement in the production of oyster mushroom.

scholarly journals Optimization of growth of oyster mushroom mycelium (Pleurotus sp.) from Tasikmalaya on several kinds of cereal medium

2019 ◽  
Vol 1 (2) ◽  
pp. 13-17
Author(s):  
Khusnul Khusnul
Keyword(s):  
Human Body ◽  
Edible Mushroom ◽  
Oyster Mushroom ◽  
Mushroom Cultivation ◽  
Mycelium Growth ◽  
Randomized Design ◽  
Growing Medium ◽  
Completely Randomized Design ◽  
Seed Medium

Pleurotus sp. or oyster mushroom is one type of edible mushroom that contains essential nutrients for the human body. This mushroom has been widely cultivated in Indonesia, one of which is Pleurotus sp. from Tasikmalaya. The key to the success of oyster mushroom cultivation is the seeds and growing medium or baglog, while the factors that influence the quality of the seeds include the type of medium. This study aims to determine 1) the effect of the type of seed medium from the type of cereals used on the growth of mycelium Pleurotus sp. from Tasikmalaya, 2) determine the type of seed medium that has the best mycelium growth. This study uses an experimental method with a Completely Randomized Design (CRD). The parameters measured include the main parameters, namely the rate of growth of mycelium Pleurotus sp. from Tasikmalaya on the seed medium. Supporting parameters are the quality of mycelium growth in the seed medium, the proximate value of cereals used for the seed medium. The results of the analysis showed that the medium of cereals used as seeds ready for cultivation could affect the quality of the seeds produced. The seedling medium from milled corn has the best growth of mycelium.

scholarly journals Evaluation of waste paper for cultivation of oyster mushroom (Pleurotus ostreatus) with some added supplementary materials

2019 ◽  
Author(s):  
Teklemichael Tesfay ◽  
Tesfay Godifey ◽  
Roman Mesfin ◽  
Girmay Kalayu
Keyword(s):  
Wheat Bran ◽  
Total Yield ◽  
Waste Paper ◽  
Oyster Mushroom ◽  
Biological Efficiency ◽  
Corn Stalk ◽  
Mushroom Cultivation ◽  
Promising Alternative ◽  
Lignocellulosic Wastes ◽  
Stalk Length

AbstractMushroom cultivation is an economically feasible bio-technological process for conversion of various lignocellulosic wastes. This study was conducted at Aksum University with the aim of evaluating the suitability of waste paper supplemented with corn stalk and wheat bran for Oyster mushroom cultivation. Spawn were prepared in Microbiology laboratory and inoculated into the prepared substrates. Waste paper supplemented with corn stalk and wheat bran with 0%, 25% and 50% were tested for their productivity and biological efficiency (BE) for cultivation of P.ostreatus mushroom. Data were analyzed using SPSS version 20. Higher (26.20± 19.36) mean weight, pileus diameter (7.90 ±2.66cm), total yield (646.4 ±273.1gm) and BE (64.64± 273 % were obtained from waste paper (50%) +cornstalk (25%) +wheat bran (25%). However, Lower (17.92±81.95%) BE were obtained from waste paper (100%). Moreover, the highest (3.88 ±0.32 cm) mean stalk length were obtained from waste paper (50%) + cornstalk (50). This study revealed that waste paper supplemented with corn stalk and wheat bran results in high BE and total yield. Thus, appears to be a promising alternative for the cultivation of oyster mushroom. Yet, waste paper without supplement poorly supports the growth of P.ostreatus mushroom.

scholarly journals Production of biocomposites from the reuse of coconut powder colonized by Shiitake mushroom

2019 ◽  
Vol 43 ◽  
Author(s):  
Maxwell Paca Matos ◽  
Juan Lopes Teixeira ◽  
Brenno Lima Nascimento ◽  
Sandro Griza ◽  
Francisco Sandro Rodrigues Holanda ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Wheat Bran ◽  
Lentinula Edodes ◽  
Oil Industry ◽  
Edible Mushroom ◽  
Mechanical Resistance ◽  
Shiitake Mushroom ◽  
Drying Time ◽  
Growing Period ◽  
Biodegradable Composite

ABSTRACT The demand for biodegradable composite has grown worldwide in recent years, mainly in order to reduce environmental contamination by structural materials produced from the oil industry. The objective of this study was to evaluate the growth of isolates from the edible mushroom “Shiitake” (Lentinula edodes) in substrate coconut powder-based supplemented with wheat bran, as well as to analyze the influence of fungi growing period and drying time of the colonized substrate on the mechanical properties of the composite, in order to produce a biodegradable composite. The mycelial density is not influenced by the type of hyphae of L. edodes. Drying of the composite does not influence the residual odor, depending on the isolate. The compressive strength and foam type of the fungal composite may be influenced by the culture period and type of hyphae, depending on the fungal isolate. The composites colonized by the L. edodes isolates presented higher mechanical resistance at 30 days of complete colonization. The coconut powder supplemented with wheat bran colonized by isolated fungi LED 96/18 is an ecological alternative in the packaging production considering its mechanical properties.

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