scholarly journals New Method for the Selection of Multicopy Transformants of Pichia pastoris, Using 3-Amino-1,2,4 Triazol

BioTechniques ◽  
2000 ◽  
Vol 29 (5) ◽  
pp. 1094-1099 ◽  
Author(s):  
Javier Menéndez ◽  
Bianca García ◽  
Yurima Hidalgo
Keyword(s):  
Pichia Pastoris ◽  
New Method ◽  
Selection Of

scholarly journals Positive Selection of Novel Peroxisome Biogenesis-Defective Mutants of the Yeast Pichia pastoris

Genetics ◽  
1999 ◽  
Vol 151 (4) ◽  
pp. 1379-1391
Author(s):  
Monique A Johnson ◽  
Hans R Waterham ◽  
Galyna P Ksheminska ◽  
Liubov R Fayura ◽  
Joan Lin Cereghino ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Allyl Alcohol ◽  
Alcohol Oxidase ◽  
Methylotrophic Yeast ◽  
Direct Selection ◽  
Toxic Exposure ◽  
Peroxisome Biogenesis ◽  
Yeast Pichia Pastoris ◽  
Methylotrophic Yeast Pichia Pastoris ◽  
Selection Of

Abstract We have developed two novel schemes for the direct selection of peroxisome-biogenesis-defective (pex) mutants of the methylotrophic yeast Pichia pastoris. Both schemes take advantage of our observation that methanol-induced pex mutants contain little or no alcohol oxidase (AOX) activity. AOX is a peroxisomal matrix enzyme that catalyzes the first step in the methanol-utilization pathway. One scheme utilizes allyl alcohol, a compound that is not toxic to cells but is oxidized by AOX to acrolein, a compound that is toxic. Exposure of mutagenized populations of AOX-induced cells to allyl alcohol selectively kills AOX-containing cells. However, pex mutants without AOX are able to grow. The second scheme utilizes a P. pastoris strain that is defective in formaldehyde dehydrogenase (FLD), a methanol pathway enzyme required to metabolize formaldehyde, the product of AOX. AOX-induced cells of fld1 strains are sensitive to methanol because of the accumulation of formaldehyde. However, fld1 pex mutants, with little active AOX, do not efficiently oxidize methanol to formaldehyde and therefore are not sensitive to methanol. Using these selections, new pex mutant alleles in previously identified PEX genes have been isolated along with mutants in three previously unidentified PEX groups.

A New Method for Selecting the Best Design

2012 ◽  
Vol 20 (1) ◽  
pp. 11-17
Author(s):  
Mark E. Benden ◽  
Kristen Miller ◽  
Eric Wilke ◽  
Eduardo Ibarra
Keyword(s):  
Patient Outcomes ◽  
New Method ◽  
Driver Safety ◽  
Transport Vehicle ◽  
Medical Transport ◽  
Expert Ratings ◽  
Vehicle Seat ◽  
Improve Patient ◽  
Individual Expert ◽  
Selection Of

In this article the authors illustrate how individual expert ratings can be employed to prioritize specifications for use in forced rankings. Those rankings are then used to select a design with the best overall usability. The authors provide an example of this approach in the selection of a medical transport vehicle seat to produce a more ergonomic product that could improve patient outcomes and driver safety.

A New Method for Selection of Escherichia coli Mutants defective in Membrane Lipid Synthesis

1972 ◽  
Vol 240 (96) ◽  
pp. 21-22 ◽  
Author(s):  
J. E. CRONAN
Keyword(s):  
Escherichia Coli ◽  
Lipid Synthesis ◽  
Membrane Lipid ◽  
New Method ◽  
Selection Of

scholarly journals First person – Natalí Delorme and Leonardo Zamora

Biology Open ◽  
2021 ◽  
Vol 10 (12) ◽  
Keyword(s):  
Oxidative Stress ◽  
Life Cycle ◽  
New Zealand ◽  
Stress Response ◽  
Marine Invertebrates ◽  
Early Career ◽  
New Method ◽  
First Person ◽  
Early Career Researchers ◽  
Selection Of

ABSTRACT First Person is a series of interviews with the first authors of a selection of papers published in Biology Open, helping early-career researchers promote themselves alongside their papers. Natalí Delorme and Leonardo Zamora are co-first authors on ‘ A new method to localise and quantify oxidative stress in live juvenile mussels’, published in BiO. They are both researchers in the laboratory of Serean Adams at the Cawthron Institute, Nelson, New Zealand. Natalí's research interests centre around ecophysiology of marine invertebrates, particularly on the organisms' stress response. Leonardo is investigating the biology of commercially, ecologically and culturally relevant marine invertebrates throughout their life cycle.

Selection of Pichia pastoris strains expressing recombinant immunoglobulin G by cell surface labeling

2010 ◽  
Vol 358 (1-2) ◽  
pp. 66-74 ◽  
Author(s):  
Song Lin ◽  
Zheng Shen ◽  
Dongxing Zha ◽  
Nathan Sharkey ◽  
Bianka Prinz ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Cell Surface ◽  
Immunoglobulin G ◽  
Selection Of

scholarly journals A new method for the selection of candidates for shading and blocking in central receiver systems

2020 ◽  
Vol 152 ◽  
pp. 961-973
Author(s):  
Guillermo Ortega ◽  
Antonio Rovira
Keyword(s):  
New Method ◽  
Central Receiver ◽  
Selection Of

Quantitative Comparison of the Shape of Bio-organic Molecules

1983 ◽  
Vol 38 (12) ◽  
pp. 1342-1345 ◽  
Author(s):  
loan Motoc
Keyword(s):  
Organic Molecules ◽  
Drug Research ◽  
Biological Action ◽  
Quantitative Comparison ◽  
New Method ◽  
Selection Of

Abstract The paper proposes and illustrates a new method for quantitatively comparing the degree of relatedness of the shape of bio-organic molecules which exhibit the same biological action but which may vary widely in their shape. The method is of interest for drug research in connection with the design and selection of test compounds.

Compared to What? A Comment on “A Robust Transformation Procedure for Interpreting Political Text” by Martin and Vanberg

2007 ◽  
Vol 16 (1) ◽  
pp. 101-111 ◽  
Author(s):  
Kenneth Benoit ◽  
Michael Laver
Keyword(s):  
Research Design ◽  
Critical Analysis ◽  
Statistical Analyses ◽  
New Method ◽  
Policy Positions ◽  
Transformation Procedure ◽  
Alternative Method ◽  
Research Designs ◽  
Original Reference ◽  
Selection Of

In “A Robust Transformation Procedure,” Martin and Vanberg (2007, hereafter MV) propose a new method for rescaling the raw virgin text scores produced by the “Wordscores” procedure of Laver, Benoit, and Garry (2003, hereafter LBG). Their alternative method addresses two deficiencies they argue exist with the transformation of virgin text scores proposed by LBG: First, that the LBG transformation is sensitive to the selection of virgin texts, and second, that it distorts the reference metric by failing to recover the original reference scores when reference texts are scored and transformed as if they were virgin texts. Their proposed alternative is “robust” in the sense that it avoids both shortcomings. Not only is MV's transformation a welcome contribution to the Wordscores project but also the critical analysis on which it is based brings to light a number of assumptions and choices that face the analyst seeking to estimate actors' policy positions using statistical analyses of the texts they generate. When first describing the possibility of rescaling the raw virgin text estimates, we emphasized that ourparticular approach to rescaling is not fundamental to our word-scoring technique but, rather, is a matter of substantive research design unrelated to the validity of the raw virgin text scores… Other transformations are of course possible. (LBG, 316)To explore more fully into the assumptions and choices behind alternative transformations and the research designs which motivate them, we offer the following comments.

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