The effect of extraction technology on antioxidant activity of black chokeberry

Manililla is a mango variety whose residues contain bioactive compounds such as polyphenols and flavonoids, with high added value. The use of environmentally friendly extraction technology would be of great relevance; hence, this study aimed to evaluate the effect of solvent relation, sonication time and amplitude on the ultrasound-assisted extraction of total polyphenols in Manililla mango residues (peel, endocarp and kernel) and antioxidant activity. An experimental design 23 with a central point was used to evaluate the curvature behavior of the process variables. Conventional maceration was used as a control. The better conditions were obtained at the central point using 50% ethanol in water, 60% amplitude and 20 min of sonication time. We obtained values of up to 1814 mg GAE/100 g, 469 mg GAE/100 g and 672 mg GAE/100 g of total polyphenols and 1228 mg QE/100 g, 653 mg QE/100 g and 880 mg QE/100 g of total flavonoids for peel, endocarp and kernel, respectively. Mangiferin was quantified in ultrasound-assisted extraction at 150 mg/g in peel and 0.025 mg/g in the kernel, but it was not detectable in maceration. An antioxidant capacity of 87%, 14% and 83% inhibition for peel, endocarp and kernel, respectively, were obtained. Peel and kernel were the residues with higher potential as extraction material, while endocarp was not.

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Optimization of the Extraction Technology and Assessment of Antioxidant Activity of Chlorogenic Acid-Rich Extracts From Eucommia ulmoides Leaves

Natural Product Communications ◽

10.1177/1934578x211046105 ◽

2021 ◽

Vol 16 (10) ◽

pp. 1934578X2110461

Author(s):

Hua Jiang ◽

Jun Li ◽

Ning Zhang ◽

Hai-Yang He ◽

Jia-Min An ◽

...

Keyword(s):

Antioxidant Activity ◽

Chlorogenic Acid ◽

Ph Value ◽

Extraction Process ◽

Eucommia Ulmoides ◽

Extraction Technology ◽

Dpph Method ◽

Contour Plots ◽

Technology And Assessment ◽

Design Optimum

Chlorogenic acid has been proved to have cardiovascular protection, antibacterial, antiviral, hemostatic, and hypolipidemia effects. Modern scientific research on the bioactivity of chlorogenic acid has been extended to the fields of food, medicine, health care and daily-use chemical industry. The aim of this research was to optimize the extraction conditions for chlorogenic acid from Eucommia ulmoides (Eucommiaceae) leaves. The significant variables were screened and optimized by a combination of Plackett-Burman test and Box-Behnken design. Optimum extraction parameters with ethanol concentration of 50%, solvent pH value of 3, and particle size of 60 mesh were determined according to variance analysis and contour plots. Under these conditions, the yield of chlorogenic acid was up to 4.36 mg/g, which was basically consistent with the theoretical prediction value of 4.50 mg/g. This study also proved the potential antioxidant activity of E. ulmoides leaves. The optimal extract of E. ulmoides leaves rich in chlorogenic acid showed the highest antioxidant activity in the FRAP method, which was 219.8 μM Trolox equivalents (TE) per g extract weight (EW) (μM TE/g EW). The DPPH method gave a similar value (168 μM TE/g EW) to the ABTS method (152 μM TE/g EW). The established extraction process was efficient in the recovery of chlorogenic acid from E. ulmoides leaves, encouraging its valorization as a cheap and sustainable alternative for the isolation of chlorogenic acid.

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Multivariate analysis of polyphenolic content and in vitro antioxidant capacity of wild and cultivated berries from Bosnia and Herzegovina

Scientific Reports ◽

10.1038/s41598-021-98896-8 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Aleksandra Marjanovic ◽

Jasmina Djedjibegovic ◽

Aida Lugusic ◽

Miroslav Sober ◽

Luciano Saso

Keyword(s):

Antioxidant Activity ◽

Antioxidant Capacity ◽

Bosnia And Herzegovina ◽

Total Phenolics ◽

Water Extract ◽

In Vitro Tests ◽

Black Currant ◽

Cornelian Cherry ◽

Black Chokeberry

AbstractThe aim of this study was to determine the antioxidant activity, total phenolics, total flavonoid, proanthocyanidins, and anthocyanins content of eight berry species, namely serviceberry, gooseberry, blackberry, black chokeberry, bilberry, red currant, black currant, and cornelian cherry harvested in the regions of Sarajevo and Tuzla, Bosnia and Herzegovina. The antioxidant activity was determined by a battery of in vitro tests including DPPH radical assay, FRAP assay, ABTS assay, and phosphomolybdate test for total antioxidant capacity. Total phenolics, total flavonoids, and proanthocyanidins ranged from 0.834 to 6.921 mg TAE (tannic acid equivalents), 0.081–0.673 mg of quercetin, and 0.162–3.247 mg of catechin per gram of fresh fruit, respectively. The water extract of fruits had considerable levels of tested constituents and antioxidant activity, with the highest results obtained for black chokeberry. The multivariate clustering analysis showed that water extracts of analyzed species of berries belong to four distinct types in terms of their antioxidants levels and antioxidant activity. Furthermore, these results support the opinion that employment of multiple antioxidant tests is indeed required for adequate in vitro assessment of antioxidant capacity. Results also emphasized the need for a more detailed evaluation of the fruit species with good antioxidant potential (relative to standards), such as cornelian cherry and gooseberry, which are abundant yet not frequently consumed in Bosnia and Herzegovina.

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Effect of extraction method on phenolic content and antioxidant activity of mistletoe extracts from Viscum album subsp. abietis

Chemical Papers ◽

10.2478/s11696-013-0524-4 ◽

2014 ◽

Vol 68 (7) ◽

Cited By ~ 14

Author(s):

Wioleta Pietrzak ◽

Renata Nowak ◽

Marta Olech

Keyword(s):

Antioxidant Activity ◽

Extraction Efficiency ◽

Antioxidant Activities ◽

Total Content ◽

Viscum Album ◽

Extraction Methods ◽

Solvent Mixtures ◽

Ultrasound Assisted Extraction ◽

Extraction Technology ◽

Considerable Saving

AbstractThe total content of polyphenols and flavonoids determined in the same plant and their corresponding antioxidant activities may vary widely, depending on the extraction conditions applied. This study was conducted to optimise the extraction conditions of phenolics and flavonoids from the mistletoe plant. Various extraction methods, i.e. ultrasound-assisted extraction technology, maceration, maceration with stirring, accelerated solvent extraction (ASE), and extraction under reflux were evaluated for their percentage extraction of polyphenols (TPC) and flavonoids (TFC) from Viscum album subsp. abietis. In addition, the anti-radical activity of extracts was analysed using the 2,2-diphenyl-1-picrylhydrazyl method. The effects of temperature, solvent type, and concentration on the phenolic extraction efficiency and antioxidant activity were studied using chemometric and statistical methods. The results showed that the extracts of V. album subsp. abietis contained large amounts of polyphenols and flavonoids (up to 57.673 mg g−1 and 9.955 mg g−1 of dry extract, respectively) and exhibited potent antioxidant activity, hence representing promising sources of powerful antioxidants. Due to its high extraction efficiency and considerable saving of time and solvent, ASE was more effective than the other extraction techniques. Extracts prepared with water-polar solvent mixtures displayed the highest TPC, TFC, and antioxidant activity, while organic polar solvents were the least efficient extractants.

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Optimization of Ultrasonic-assisted Extraction Technology of Sargassum fusiforme Polysaccharides and Evaluation of Their Antioxidant Activity

Food Science and Technology Research ◽

10.3136/fstr.19.157 ◽

2013 ◽

Vol 19 (2) ◽

pp. 157-162 ◽

Cited By ~ 4

Author(s):

Caiyan LI ◽

Caisheng WANG ◽

Sufang WANG ◽

Guoying QIAN ◽

Qiuhua ZHU ◽

...

Keyword(s):

Antioxidant Activity ◽

Extraction Technology ◽

Sargassum Fusiforme ◽

Assisted Extraction ◽

Ultrasonic Assisted ◽

Ultrasonic Assisted Extraction

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Aronia melanocarpa Fruit and Leaves Hot-Assisted Ethanolic Extracts Antioxidant Activity

Proceedings ◽

10.3390/proceedings2020057057 ◽

2020 ◽

Vol 57 (1) ◽

pp. 57

Author(s):

Andrei Munteanu ◽

Alin Enache ◽

Georgeta Neagu ◽

Corina Bubueanu ◽

Alice Grigore ◽

...

Keyword(s):

Antioxidant Activity ◽

Quinic Acid ◽

Antioxidant Compounds ◽

Total Phenols ◽

Aronia Melanocarpa ◽

Fresh Material ◽

Ethanolic Extracts ◽

Black Chokeberry ◽

Caffeoyl Quinic Acid

Aronia melanocarpa L. fruit (common black chokeberry) is one of the most abundant sources of antioxidant compounds in the plant world, superior to all edible fruits; chokeberry fruits contain up to 100 g total phenols per kg fresh material, predominantly (−)epicatechin, cyanidin-3-glycosides and procyanidins (60%), added to quercetin and caffeoyl quinic acid derivates.

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Optimization of Microwave-Assisted Extraction of Polysaccharides from Ulva pertusa and Evaluation of Their Antioxidant Activity

Antioxidants ◽

10.3390/antiox8050129 ◽

2019 ◽

Vol 8 (5) ◽

pp. 129 ◽

Cited By ~ 11

Author(s):

Bao Le ◽

Kirill S. Golokhvast ◽

Seung Hwan Yang ◽

Sangmi Sun

Keyword(s):

Antioxidant Activity ◽

Superoxide Dismutase ◽

Raw Material ◽

Ulva Pertusa ◽

Extraction Time ◽

Oxidative Enzymes ◽

Microwave Assisted Extraction ◽

Extraction Technology ◽

Microwave Assisted ◽

Assisted Extraction

The use of green marine seaweed Ulva spp. as foods, feed supplements, and functional ingredients has gained increasing interest. Microwave-assisted extraction technology was employed to improve the extraction yield and composition of Ulva pertusa polysaccharides. The antioxidant activity of ulvan was also evaluated. The impacts of four independent variables, i.e., extraction time (X1, 30 to 60 min), power (X2, 500 to 700 W), water-to-raw-material ratio (X3, 40 to 70), and pH (X4, 5 to 7) were evaluated. The chemical structure of different polysaccharides fractions was investigated via FT-IR and the determination of their antioxidant activities. A response surface methodology based on a Box–Behnken design (BBD) was used to optimize the extraction conditions as follows: extraction time of 43.63 min, power level of 600 W, water-to-raw-material ratio of 55.45, pH of 6.57, and maximum yield of 41.91%, with a desired value of 0.381. Ulvan exerted a strong antioxidant effect against 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and showed reducing power in vitro. Ulvan protected RAW 264.7 cells against H2O2-induced oxidative stress by upregulating the expression and enhancing the activity of oxidative enzymes such as superoxide dismutase (SOD) and superoxide dismutase (CAT). The results suggest that the polysaccharides from U. pertusa might be promising bioactive compounds for commercial use.

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