In vivo biodistribution, anti-inflammatory, and hepatoprotective effects of liver targeting dexamethasone acetate loaded nanostructured lipid carrier system

Lead is one of the most common environmental contaminants in the Earth’s crust, which induces a wide range of humans biochemical changes. Previous studies showed that Opuntia dillenii (OD) fruit possesses several antioxidant and anti-inflammatory properties. The present study evaluates OD fruit hydroalcoholic extract (OHAE) hepatoprotective effects against lead acetate- (Pb-) induced toxicity in both animal and cellular models. Male rats were grouped as follows: control, Pb (25 mg/kg/d i.p.), and groups 3 and 4 received OHAE at 100 and 200 mg/kg/d + Pb (25 mg/kg/d i.p.), for ten days of the experiment. Thereafter, we evaluated the levels of alkaline phosphatase (ALP), alanine aminotransferase (ALT), and aspartate aminotransferase (AST), catalase (CAT) activity and malondialdehyde (MDA) in serum, and liver histopathology. Additionally, the cell study was also done using the HepG2 cell line for measuring the direct effects of the extract on cell viability, oxidative stress MDA, and glutathione (GSH) and inflammation tumor necrosis factor-α (TNF-α) following the Pb-induced cytotoxicity. Pb significantly increased the serum levels of ALT, AST, ALP, and MDA and liver histopathological scores but notably decreased CAT activity compared to the control group ( p < 0.001 for all cases). OHAE (100 and 200 mg/kg) significantly reduced the levels of serum liver enzyme activities and MDA as well as histopathological scores while it significantly increased CAT activity compared to the Pb group ( p < 0.001 –0.05 for all cases). OHAE (20, 40, and 80 μg/ml) concentration dependently and significantly reduced the levels of MDA and TNF-α, while it increased the levels of GSH and cell viability in comparison to the Pb group ( p < 0.001 –0.05 for all cases). These data suggest that OHAE may have hepatoprotective effects against Pb-induced liver toxicity both in vitro and in vivo by its antioxidant and anti-inflammatory activities.

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Curcumin loaded and co-loaded nanosystems: A review from a biological activity enhancement perspective

Pharmaceutical Nanotechnology ◽

10.2174/2211738508666201228150659 ◽

2020 ◽

Vol 08 ◽

Author(s):

Andrea Mariela Araya-Sibaja ◽

Krissia Wilhelm ◽

Gustavo Adolfo González-Aguilar ◽

José Roberto Vega-Baudrit ◽

Norma Julieta Salazar-López ◽

...

Keyword(s):

Central Nervous System ◽

Nervous System ◽

Aqueous Solubility ◽

Nanostructured Lipid Carrier ◽

Protective Effects ◽

Cell Internalization ◽

Low Toxicity ◽

Anti Inflammatory

Background: Curcumin is a natural phenolic compound exhibiting multiple bioactivities that have been evaluated in vitro, in vivo as well as through clinical studies in humans. Some of them include antimicrobial, antioxidant, anti-inflammatory and central nervous system protective effects. Further, curcumin is considered a Generally Recognized as Safe substance because of its low toxicity. However, its molecular structure is susceptible to changes in pH, oxidation, photodegradation, low aqueous solubility and biotransformation compromising its bioavailability, drawbacks that have been successfully addressed through nanotechnology. Objective: The present review systematizes findings on the enhancement of curcumin’s beneficial effects when it is loaded and co-loaded into different types of nanosystems covering liposomes, polymeric and solid-lipid nanoparticles, nanostructured lipid carrier, lipid-polymeric hybrids, self-assembled and protein-based core-shell systems in relation to its antimicrobial, antioxidant, anti-inflammatory and central nervous system protective bioactivities. Conclusion: Curcumin is a versatile molecule capable of exerting antimicrobial, antioxidant, anti-inflammatory and central nervous system protective effects in an enhanced manner using the possibilities offered by the nanotechnology - based approach. Its enhanced bioactivities are associated with increments in solubility, stability, bioavailability as well as in improved intracellular uptake and cell internalization. These advantages, in addition to curcumin’s low toxicity, indicate the potential of curcumin to be loaded and co-loaded into nanosystems capable to provide a controlled release and targeted administration.

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Nanostructured lipid carrier improved in vivo anti-tumor and immunomodulatory effect of Zerumbone in 4T1 challenged mice

RSC Advances ◽

10.1039/c5ra00144g ◽

2015 ◽

Vol 5 (28) ◽

pp. 22066-22074 ◽

Cited By ~ 14

Author(s):

Nurul Elyani Mohamad ◽

Nadiah Abu ◽

Heshu Sulaiman Rahman ◽

Huynh Ky ◽

Wan Yong Ho ◽

...

Keyword(s):

Antitumor Immunity ◽

Immunomodulatory Effect ◽

Nanostructured Lipid Carrier ◽

Anti Inflammatory

Nanostructured lipid carrier (NLC) encapsulation enhanced thein vivoantitumor, antitumor immunity and anti-inflammatory effects of Zerumbone on 4T1 challenged mice.

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Engineering of glycyrrhizin capped gold nanoparticles for liver targeting: in vitro evaluation and in vivo biodistribution study

RSC Advances ◽

10.1039/c6ra05202a ◽

2016 ◽

Vol 6 (51) ◽

pp. 44944-44954 ◽

Cited By ~ 5

Author(s):

Shaivee Borker ◽

Milind Patole ◽

Alpana Moghe ◽

Varsha Pokharkar

Keyword(s):

Gold Nanoparticles ◽

Antiviral Drug ◽

Biodistribution Study ◽

Liver Targeting ◽

In Vitro Evaluation ◽

In Vivo Biodistribution

Glycyrrhizin reduced and stabilized gold nanoparticles as carriers of antiviral drug lamivudine. The presence of glycyrrhizin enhanced uptake and localization of drug loaded gold nanoparticles in hepatocytes.

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