`Objective: To know the prevalence of ABO and Rhesus blood groups.Design: Observational cross sectional study. Setting: Blood transfusion Center Tehsil Headquarter HospitalLiaquatpur. Subjects and Methods: The data of blood donors from 2001 to 2003. Results: Total numbers of donorsstudied were 1389. The commonest ABO blood group was O present in 44.56% (CL 41.97% - 47.20%) followed byB in 32.54% (CL 30.13% - 35.06%), A in 20.88% (CL 18.82% - 23.10%) and AB in 2.02% (CL 1.39% - 2.91%) donorswhile 90.35% (CL 88.68% - 91.80%) donors were Rh +ve and 9.65% (CL 8.20% - 11.32%) were Rh -ve. Thecommonest ABO +ve group was O +ve present in 40.03% (CL 37.48% - 42.69%) followed by B +ve in 30.31% (CL27.95% - 32.78%), A +ve in 18.21% (CL 16.27% - 20.34%) and AB +ve in 1.8% (CL 1.21% - 2.66%) donors. The mostcommon ABO -ve was O -ve present in 4.54% (CL 3.56% - 5.77%), A -ve in 2.66% (CL 1.93% - 3.66%), B -ve in 2.23%(CL 1.57% - 3.17%) donors. Conclusion: Blood group O is the commonest ABO blood group and 90.35% are RH +vein this area.