scholarly journals Vibrio fischeri and Escherichia coli adhesion tendencies towards photolithographically modified nanosmooth poly (tert-butyl methacrylate) polymer surfaces

2008 ◽  
Vol Volume 1 ◽  
pp. 33-44 ◽  
Author(s):  
Elena Ivanova
Keyword(s):  
Escherichia Coli ◽  
Vibrio Fischeri ◽  
Polymer Surfaces ◽  
Butyl Methacrylate

Degradation and changes in toxicity and biodegradability of tetracycline during ozone/ultraviolet-based advanced oxidation

2014 ◽  
Vol 70 (7) ◽  
pp. 1229-1235 ◽  
Author(s):  
Huyen Trang Luu ◽  
Kisay Lee
Keyword(s):  
Escherichia Coli ◽  
Biological Treatment ◽  
Advanced Oxidation Processes ◽  
Vibrio Fischeri ◽  
Oxygen Demand ◽  
Advanced Oxidation ◽  
Uv Treatment ◽  
Experimental Conditions ◽  
Oxidation Processes ◽  
Complete Mineralization

Advanced oxidation processes (AOPs) composed of O3, H2O2 and ultraviolet (UV) were applied to degrade tetracycline (TC). Degradation efficiency was evaluated in terms of changes in absorbance (ABS) and total organic carbon (TOC). The change in biotoxicity was monitored with Escherichia coli and Vibrio fischeri. The improvement in biodegradability during oxidation was demonstrated through 5-day biochemical oxygen demand/chemical oxygen demand ratio and aerobic biological treatment. The combination of O3/H2O2/UV and O3/UV showed the best performance for the reductions in ABS and TOC. However, mineralization and detoxification were not perfect under the experimental conditions that were used in this study. Therefore, for the ultimate treatment of TC compounds, it is suggested that AOP treatment is followed by biological treatment, utilizing enhanced biodegradability. In this study, aerobic biological treatment by Pseudomonas putida was performed for O3/UV-treated TC. It was confirmed that O3/UV treatment improved TOC reduction and facilitated complete mineralization in biological treatment.

Involvement of host factors in the regulation of the Vibrio fischeri lux operon in Escherichia coli cells

Microbiology ◽  
2006 ◽  
Vol 75 (4) ◽  
pp. 452-458 ◽  
Author(s):  
I. V. Manukhov ◽  
V. Yu. Kotova ◽  
G. B. Zavil’gel’sky
Keyword(s):  
Escherichia Coli ◽  
Vibrio Fischeri ◽  
Host Factors ◽  
Escherichia Coli Cells ◽  
Lux Operon

H-NS Protein Represses Transcription of the lux Systems of Vibrio fischeri and Other Luminous Bacteria Cloned into Escherichia coli

1997 ◽  
Vol 35 (6) ◽  
pp. 336-342 ◽  
Author(s):  
Shimon Ulitzur ◽  
Abdul Matin ◽  
Cress Fraley ◽  
Edward Meighen
Keyword(s):  
Escherichia Coli ◽  
Vibrio Fischeri ◽  
Luminous Bacteria

Bacteriophage-Based Bioluminescent Bioreporter for the Detection of Escherichia coli O157:H7

2007 ◽  
Vol 70 (6) ◽  
pp. 1386-1392 ◽  
Author(s):  
JENNIFER R. BRIGATI ◽  
STEVEN A. RIPP ◽  
COURTNEY M. JOHNSON ◽  
POLINA A. IAKOVA ◽  
PATRICIA JEGIER ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pathogenic Bacteria ◽  
Vibrio Fischeri ◽  
Homoserine Lactone ◽  
Escherichia Coli O157 ◽  
Recombinant Phage ◽  
E Coli ◽  
Lux Operon ◽  
Multiple Sample ◽  
New Bioassay

The rapid detection of pathogenic bacteria in food and water is vital for the prevention of foodborne illness. In this study, the lux reporter genes were used in a new bioassay that allows pathogen monitoring without multiple sample manipulations or the addition of exogenous substrate. A recombinant phage specific for Escherichia coli O157:H7 was constructed that, upon infection, catalyzes the synthesis of N-(3-oxohexanoyl)-L-homoserine lactone (OHHL). This phage PP01 derivative carries the luxI gene from Vibrio fischeri under the control of the phage promoter PL. OHHL produced by infected E. coli O157:H7 induces bioluminescence in bioreporter cells carrying the V. fischeri lux operon. The ability of phage PP01-luxI to detect several strains of E. coli O157:H7 was confirmed in a 96-well plate assay. In this assay, luxCDABE bioreporter cells capable of detecting OHHL were mixed with phage PP01-luxI and E. coli O157:H7, and luminescence was monitored. Reporter phages induced light in bioreporter cells within 1 h when exposed to 104 CFU/ml of E. coli O157:H7 and were able to detect 10 CFU/ml in pure culture with a preincubation step (total detection time, 4 h). The detection method was also applied to contaminated apple juice and was able to detect 104 CFU/ml of E. coli O157:H7 in 2 h after a 6-h preincubation.

scholarly journals Adhesion and Proliferation of Human Periodontal Ligament Cells on Poly(2-methoxyethyl acrylate)

2014 ◽  
Vol 2014 ◽  
pp. 1-14 ◽  
Author(s):  
Erika Kitakami ◽  
Makiko Aoki ◽  
Chikako Sato ◽  
Hiroshi Ishihata ◽  
Masaru Tanaka
Keyword(s):  
Tissue Engineering ◽  
Periodontal Ligament ◽  
Polymer Surfaces ◽  
Butyl Methacrylate ◽  
Periodontal Ligament Cells ◽  
Hydroxyethyl Methacrylate ◽  
Pdl Cells ◽  
Human Blood Cells ◽  
Initial Adhesion ◽  
Cell Source

Human periodontal ligament (PDL) cells obtained from extracted teeth are a potential cell source for tissue engineering. We previously reported that poly(2-methoxyethyl acrylate) (PMEA) is highly biocompatible with human blood cells. In this study, we investigated the adhesion, morphology, and proliferation of PDL cells on PMEA and other types of polymers to design an appropriate scaffold for tissue engineering. PDL cells adhered and proliferated on all investigated polymer surfaces except for poly(2-hydroxyethyl methacrylate) and poly[(2-methacryloyloxyethyl phosphorylcholine)-co-(n-butyl methacrylate)]. The initial adhesion of the PDL cells on PMEA was comparable with that on polyethylene terephthalate (PET). In addition, the PDL cells on PMEA spread well and exhibited proliferation behavior similar to that observed on PET. In contrast, platelets hardly adhered to PMEA. PMEA is therefore expected to be an excellent scaffold for tissue engineering and for culturing tissue-derived cells in a blood-rich environment.

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