scholarly journals Influence of different ex vivo cell culture methods on the proliferation and anti-tumor activity of cytokine-induced killer cells from gastric cancer patients

2018 ◽  
Vol Volume 11 ◽  
pp. 2657-2672
Author(s):  
Bin Shi ◽  
Aixia Sun ◽  
Xiaorui Zhang
Keyword(s):  
Gastric Cancer ◽  
Cell Culture ◽  
Cancer Patients ◽  
Ex Vivo ◽  
Culture Methods ◽  
Killer Cells ◽  
Gastric Cancer Patients ◽  
Anti Tumor Activity ◽  
Cell Culture Methods

scholarly journals Immune Cells Expansion From Peripheral Blood of Patients of Three Leading Cancers in Vietnam

2021 ◽  
Vol 37 (3) ◽  
Author(s):  
Chu Thi Thao ◽  
Bui Viet Anh ◽  
Nguyen Van Phong ◽  
Nguyen Trung Kien ◽  
Nguyen Dac Tu ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cell Viability ◽  
Cancer Treatment ◽  
Cancer Patients ◽  
Cell Expansion ◽  
Immune Cell ◽  
Nk Cell ◽  
Ex Vivo ◽  
Cell Number ◽  
Gastric Cancer Patients

Immunotherapy recently attracted the high attention of scientists in cancer treatment. However, this therapy is poorly studied and applied in Vietnam. In this report, we present the results of immune cell ex vivo expansion for cancer treatment. After 20-21 days of culture, the average number of CD3+CD8+ cytotoxic T lymphocytes (CTLs) increased by 633.6-fold, equivalent to 3277.5 × 106 cells, and with the cell viability was 96.3%. Among them, the increasing fold of lung, liver and gastric cancer patients were 232.4, 812.4 and 655.6 respectively. Meanwhile, the average number of CD3-CD56+ NK cells increased by 940.3-fold, equivalent to 2659.3 × 106 cells, with the cell viability of 95%. Gastric cancer patients had the highest fold of Natural killer (NK) cell expansion (1644.9 fold) compared to that in lung cancer (695.0 fold) and liver cancer patients (358.3 fold). These results revealed that our method of immune cell expansion met the requirements in the immune cell number for clinical applications in cancer treatment in Vietnam.

scholarly journals Increased apoptosis and elevated Fas expression in circulating natural killer cells in gastric cancer patients

2012 ◽  
Vol 16 (4) ◽  
pp. 473-479 ◽  
Author(s):  
Hiroaki Saito ◽  
Seigo Takaya ◽  
Tomohiro Osaki ◽  
Masahide Ikeguchi
Keyword(s):  
Gastric Cancer ◽  
Natural Killer Cells ◽  
Natural Killer ◽  
Cancer Patients ◽  
Killer Cells ◽  
Gastric Cancer Patients

Impaired IFN-γ production after stimulation with bacterial components by natural killer cells from gastric cancer patients

2011 ◽  
Vol 317 (6) ◽  
pp. 849-858 ◽  
Author(s):  
Åsa Lindgren ◽  
Cheol-Heui Yun ◽  
Åsa Sjöling ◽  
Camilla Berggren ◽  
Jia-Bin Sun ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Natural Killer Cells ◽  
Natural Killer ◽  
Cancer Patients ◽  
Killer Cells ◽  
Ifn Γ ◽  
Gastric Cancer Patients

CLINICAL SIGNIFICANCE OF SERUM CAVEOLIN-1 LEVELS IN GASTRIC CANCER PATIENTS

2018 ◽  
Vol 40 (4) ◽  
pp. 323-327 ◽  
Author(s):  
F Tas ◽  
S Karabulut ◽  
K Erturk ◽  
D Duranyildiz
Keyword(s):  
Gastric Cancer ◽  
Cancer Patients ◽  
Histological Grade ◽  
Clinical Importance ◽  
Disease Stage ◽  
Control Group ◽  
Tissue Samples ◽  
Caveolin 1 ◽  
Study Results ◽  
Gastric Cancer Patients

Aim: Caveolin-1 plays a significant role in the pathogenesis of various carcinomas and its expression affects the survival of cancer patients. However, the molecular function of caveolin-1 and its possible clinical importance has remained uncertain in gastric cancer. No clinical trial has examined serum caveolin-1 levels in gastric cancer patients so far, instead all available results were provided from studies conducted on tissue samples. In the current study, we analyzed the soluble serum caveolin-1 levels in gastric cancer patients, and specified its associations with the clinical factors and prognosis. Material and Methods: Sixty-three patients with pathologically confirmed gastric cancer were enrolled into the trial. Serum caveolin-1 concentrations were detected by ELISA method. Thirty healthy subjects were also included in the study. Results: The median age of patients was 62 years, ranging from 28 to 82 years. The serum caveolin-1 levels in gastric cancer patients were significantly higher than those in control group (p < 0.001). The common clinical parameters including patient age, sex, lesion localization, histopathology, histological grade, disease stage, and various serum tumor markers (e.g. LDH, CEA, and CA 19.9) were not found to be associated with serum caveolin-1 levels (p > 0.05). Similarly, no correlation existed between serum caveolin-1 concentration and chemotherapy responsiveness (p = 0.93). Furthermore, serum caveolin-1 level was not found to have a prognostic role (p = 0.16). Conclusion: Even though it is neither predictive nor prognostic, serum caveolin-1 level may be a valuable diagnostic indicator in patients with gastric cancer. Key

DETERMINATION OF P53 GENE CODON 72 POLYMORPHISMS IN PATIENTS WITH GASTRIC CANCER

2013 ◽  
pp. 11-17
Author(s):  
Thi Tuy Ha Nguyen ◽  
Thi Minh Thi Ha
Keyword(s):  
Gastric Cancer ◽  
Cancer Patients ◽  
P53 Gene ◽  
Diffuse Gastric Cancer ◽  
Codon 72 ◽  
Cancer Group ◽  
Pcr Rflp ◽  
Gastric Cancer Patients ◽  
The Relationship

Background: The role of p53 gene in the gastric cancer is still controversial. This study is aimed at determining the rate of the p53 gene codon 72 polymorphisms in gastric cancer patients and evaluating the relationship between these polymorphisms and endoscopic and histopathological features of gastric cancer. Patients and methods: Sixty eight patients with gastric cancer (cases) and one hundred and thirty six patients without gastric cancer (controls) were enrolled. p53 gene codon 72 polymorphisms were determined by PCR-RFLP technique with DNA extracted from samples of gastric tissue. Results: In the group of gastric cancer, Arginine/Argnine, Arginine/Proline and Proline/Proline genotypes were found in 29.4%, 42.7% and 27.9%, respectively. The differences of rates were not statistically significant between cases and controls (p > 0,05). In males, the Proline/Proline genotype was found in 38.1% in patients with gastric cancer and more frequent in patients without gastric cancer (15.7%, p = 0,01). An analysis of ROC curve showed that the cut-off was the age of 52 in the Proline/Proline genotype, but it was 65 years old in the Arginine/Proline genotype. The Proline/Proline genotype was found in 41.9% in Borrmann III/IV gastric cancer, this rate was higher than Borrmann I/II gastric cancer (16.2%, p = 0.037) and also higher than controls (18.4%, p = 0,01). The rate of Proline/Proline genotype was 41.7% in the diffuse gastric cancer, it was higher than in controls (p = 0,023). Conclusion: No significative difference of rate was found in genotypes between gastric cancer group and controls. However, there was the relationship between Proline/Proline genotype and gastric cancer in males, Borrmann types of gastric cancer, the diffuse gastric cancer. Key words: polymorphism, codon 72, p53 gene, PCR - RFLP, gastric cancer.

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