scholarly journals Impact of various factors on in vitro investigation of rare mangrove species Sonneratia apetala Buch.-Ham. (Myrtales: Lythraceae) and Suaeda maritima (L.) Dumort (Caryophyllales: Amaranthaceae)

2016 ◽  
Vol 3 (5) ◽  
pp. 181
Author(s):  
Abdul Kader ◽  
Sankar Narayan Sinha ◽  
Parthadeb Ghosh
Keyword(s):  
Callus Formation ◽  
Monsoon Season ◽  
Gene Isolation ◽  
Nodal Segments ◽  
Mangrove Species ◽  
Sonneratia Apetala ◽  
Suaeda Maritima ◽  
In Vitro Investigation ◽  
Callus Initiation

Recent studies have showed the importance and destruction of mangroves. So their restoration through tissue culture study is urgently required because in vivo propagation is plagued with unforeseen obstacles. In vitro investigation of mangroves found to be suitable material for salt tolerant mechanism studies and anti stress gene isolation. This study describes for the first time in vitro approach for rare species Sonneratia apetala Buch.-Ham. (Myrtales: Lythraceae) and herb species Suaeda maritima (L.) Dumort (Caryophyllales: Amaranthaceae) through callus. For this investigation, as a source of explants various part (leaf, intermodal and nodal segments) of both mangroves were used. These explants were cultured in various types of media (MS, LS, WPM, X and B5) with different combination of phytohormones (2, 4-D and NAA with BAP in combination). We also examined the effect of NaCl and seasons on callus initiation and growth. The highest rate of callus formation was obtained with nodal explants in MS medium supplemented with 1.5 mg.L^-1 NAA and 0.5 mg.L^-1 BAP in combination and 1 mg.L^-1 NAA and 0.5 mg.L^-1 BAP in combination for Sonneratia apetala and Suaeda maritime, respectively. We also found that callus initiation rate and growth decreased with increasing NaCl concentration higher than 80 mM and 120 mM for Sonneratia apetala and Suaeda maritima, respectively in MS media. This study also found that monsoon season was best time for invitro investigation of mangroves. The results presented here give an insight into the development of in vitro investigation suitable for mangroves. The initiated callus could be restored in low salaine or devoid of salaine land.

scholarly journals Induction, Growth Kinetics and Morpho-histological Characterization of Neem Callus

2018 ◽  
Vol 10 (6) ◽  
pp. 283
Author(s):  
Leila Albuquerque Resende de Oliveira ◽  
Annie Carolina Araújo de Oliveira ◽  
Caroline De Araújo Machado ◽  
Milena Nascimento Cardoso ◽  
Fernanda Vieira Santana ◽  
...  
Keyword(s):  
Cell Division ◽  
Azadirachta Indica ◽  
Culture Medium ◽  
Callus Formation ◽  
Nodal Segments ◽  
Insecticidal Action ◽  
Histological Characteristics ◽  
Light Brown Color

Azadirachta indica A. Juss, popularly known as neem, is a species native to India, belonging to family Meliaceae, considered the most important plant species with insecticidal action. The aim of this study was to evaluate the influence of growth regulators on induction and growth of neem callus and to observe their viability for embryogenesis through morpho-histological characteristics. In vitro germinated plants were used for excision of nodal explants. These segments were inoculated in Murashige and Skoog culture medium containing 1.0 mg/l 2,4-D (2,4-dichlorophenoxyacetic) combined with BAP (6-benzylaminopurine) at the following concentrations: 0.0, 0.5, 1.0 and 2.0 mg/l (T1, T2, T3 and T4 respectively), for callus induction. At 0 (mass of nodal segments without callus), 20, 40 and 60 days of culture, the percentage of callus formation was observed and the callus weight was measured for each treatment and at the end of the 60 days, consistency, color, and cell histology were evaluated. There was callus formation in all treatments tested. The highest induction of Azadirachta indica A. Juss callus is observed in the presence of 1.0 mg/l 2,4-D + 2.0 mg/l BAP, with callus showing light brown color, friable consistency and rounded cells with intense cell division, typical of cells with potential embryogenic capacity.

scholarly journals MICROPROPAGATTON OF ACER GINNALA.

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1095a-1095 ◽  
Author(s):  
Yrina P. Ferreras ◽  
Harry Jan Swartz
Keyword(s):  
Callus Formation ◽  
Shoot Proliferation ◽  
Shoot Elongation ◽  
Axillary Buds ◽  
Gelling Agent ◽  
Nodal Segments ◽  
Explant Orientation ◽  
In Vitro Shoots ◽  
Acer Ginnala

Shoot proliferation of Acer ginnala Maxim. (Amur maple) from nodal-segments was obtained on Murashige and Skoog salts and vitamins supplemented with 25 nM thidiazuron and 3% sucrose. Higher concentrations of cytokinin resulted in callus formation at the base of explants. Explant orientation had a significant effect on shoot elongation. Explant elongation and proliferation were correlated. Plants inverted in the medium elongated and proliferated readily. Branching was obtained primarily from axillary buds several nodes basal to the apex. Gelling agent type did not affect proliferation. Vitrification was significantly affected by type of gelling agent, gelling agent concentration and thidiazuron concentration. In vitro shoots rooted readily even in medium containing adenine. Greater than 95% of the in vitro plants established in the greenhouse.

scholarly journals In vitro propagation from nodal segments of Lippia origanoides (chemotype A)

2022 ◽  
Vol 52 (7) ◽  
Author(s):  
Julián Sebastián Ramírez Moreno ◽  
Sergio Andrés Vega Porras ◽  
Roosevelt Humberto Escobar ◽  
Elena E. Stashenko ◽  
Jorge Luis Fuentes Lorenzo ◽  
...  
Keyword(s):  
Plant Growth ◽  
Gibberellic Acid ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Callus Formation ◽  
Nodal Segments ◽  
Ms Medium ◽  
Shoot Number ◽  
Murashige And Skoog Medium

ABSTRACT: This research described an efficient micropropagation protocol for Lippia origanoides (Verbenaceae). Sterile seeds were used to obtain germinated seedlings in Murashige and Skoog medium (MS) supplemented with sucrose and agar. The nodal segments obtained from seedlings were grown on MS medium supplemented with different concentrations of gibberellic acid (GA), benzylaminopurine (BAP) and 1-naphthalenacetic acid (NAA) with BAP. The callus induction, shoots length, shoots number and root length, were analyzed. The treatments showed high percentage of callus formation at 0.5 to 1.5 mg L-1 of BAP alone or in combination with NAA (0.1 mg L-1). The highest value of shoot number per nodal segments was obtained at 1.5 mg L-1 of BAP (4.3 ± 0.8). The obtained plantlets were better rooted in vitro in the absence of plant growth regulators (PGRs) and they showed acclimatization rate of 90%. We reported a protocol for in vitro propagation and acclimatization of L. origanoides for A chemotypes from Colombia.

scholarly journals Silicon in the Embriogenic Potential of Callus in vitro of Passiflora edulis

2018 ◽  
Vol 10 (5) ◽  
pp. 345
Author(s):  
Gabrielen De Maria Gomes Dias ◽  
David Correia Dos Anjos ◽  
Bárbara Nogueira De Souza ◽  
Moacir Pasqual ◽  
Bruno Grossi Costa Homem ◽  
...  
Keyword(s):  
Culture Medium ◽  
Growth Parameters ◽  
Callus Formation ◽  
Passion Fruit ◽  
Nodal Segments ◽  
X Rays ◽  
Passiflora Edulis ◽  
Embryogenic Cells ◽  
Embryogenic Potential

Adding silicon to the culture medium may contribute, to improve many growth parameters including embryogenesis and organogenesis. The objective of this study was to evaluate the embryogenic potential of passion fruit in vitro (Passiflora edulis f. Flavicarpa O. Deg), submitted to different concentrations of silicon. Nodal segments of plants were inoculated at five concentrations of silicic acid added to the culture medium, 0.0; 0.5; 1.0; 1.5 and 2.0 g L-1. After 60 days, the visual characteristics (% contamination, callus, bud, root and oxidation), cytochemical test and microanalysis of X-rays evaluated. Concentrations of 0.5; 1.0 and 2.0 g L-1 of silicon did not differ in relation to callus formation. By the cytochemical test, all treatments presented embryogenic cells indicated by the reaction to acetic Carmine and, little reaction to the Evans blue associated with cell death. By the microanalysis of X-rays, the nutrients sulfur and phosphorus, presented significant absorption responses at the concentration of 1.5 g L-1 of silicon in comparison to the other concentrations. The silicon added to the culture medium has a response in the formation of cells with embryogenic potential in nodal segments of passion fruit. The concentration of 2.0 g L-1 of silicon acid (H4SiO4) has a high embryogenic potential in passionflower cells, Passiflora edulis f. Flavicarpa O. Deg.

scholarly journals Some important aspects in Moringa oleifera Lam. micropropagation

2019 ◽  
Vol 113 (1) ◽  
pp. 13
Author(s):  
Abdullah EL-NAGISH ◽  
Ahmed HASSANEIN ◽  
Jehan SALEM ◽  
Fayza FAHEED
Keyword(s):  
Callus Formation ◽  
Staining Intensity ◽  
Nodal Segments ◽  
Chemical Components ◽  
Nodal Segment ◽  
High Concentration ◽  
Plant Materials ◽  
Half Strength ◽  
Better Than

<p>Type and source of explant as well as the type of cytokinin were important factors for successful moringa micropropagation. Explants obtained from <em>in vitro</em> grown plant materials were better than others obtained from soil growing seedlings. In addition, nodal segments were better than shoot cuttings in terms of number of shoots/ explants, frequency of shoot formation and number of nodes/shoot. While callus formation on the base of nodal segment on BAP containing media were higher than those of KIN, especially under the influence of high concentration as an aspect of vitrification, BAP was better than KIN in moringa multiplication. Low nutrient medium (half strength MS) supplemented with 0.5 mg l<sup>-1</sup> IAA was essential for successful root formation. The suitable conditions for moringa micropropagation on full strength MS or SH may exert low stress and low need to raise the expression of SOD and POX. On the other side, stress due to over increase of chemical components of double MS medium or low nutrient content of half strength MS, B5 or WPM expressed the highest number and staining intensity of SOD and POX bands, vice versa was detected in case of CAT.</p>

scholarly journals Conservação in vitro de Erythrina velutina a partir de embriões zigóticos

2015 ◽  
Vol 15 ◽  
Author(s):  
Priscila Tavares Fonseca ◽  
Cristina Ferreira Nepomuceno ◽  
Tecla Dos Santos Silva ◽  
José Raniere Ferreira de Santana
Keyword(s):  
Natural Resources ◽  
Callus Formation ◽  
Germination Percentage ◽  
Shoot Length ◽  
Nodal Segments ◽  
Zygotic Embryos ◽  
Leaves And Roots ◽  
Number Of Shoots

Erythrina velutina is native from the Caatinga domain, which has been heavily affected by the exploitation of natural resources. Thus, the aim of this study was to evaluate the induction of buds from nodal segments of in vitro germinated plants, aiming at the conservation of E. velutina. In the experiment I, intact seeds and zygotic embryos were inoculated in WPM culture and the number of buds, leaves and roots, germination percentage, shoot length and percentage of normal plants were analyzed. In the experiment II, the nodal segments of plants from germination of zygotic embryos in vitro were inoculated in WPM culture supplemented with BAP and CIN [0.0, 10.0, 20.0, 30.0 and 40.0 µM] and percentage of responsive explants to form buds, number of shoots and leaves, shoot length and the percentage of callus formation were analyzed. The cultivation of zygotic embryos provided more vigorous and uniform plants, with more gems than those obtained from the germination of intact seeds. The concentration of 20 µM of BAP is indicated for the regeneration of plants from zygotic embryos of E. velutina; with this concentration, an average of 2.78 shoots/explant was obtained.

In vitro investigation of Varizella zoster virus as an oncolytic virus in glioblastoma therapy

2008 ◽  
Vol 35 (S 01) ◽  
Author(s):  
H Leske ◽  
A Baiker ◽  
C Schichor ◽  
J.C Tonn ◽  
R Goldbrunner ◽  
...  
Keyword(s):  
Oncolytic Virus ◽  
In Vitro Investigation ◽  
Varizella Zoster Virus

Synthetic Seed Technology for Encapsulation and Regrowth of in vitro Derived Stevia rebaudiana Bert. nodal Segments

Planta Medica ◽  
2013 ◽  
Vol 79 (05) ◽  
Author(s):  
H Lata ◽  
S Chandra ◽  
IA Khan
Keyword(s):  
Stevia Rebaudiana ◽  
Nodal Segments ◽  
Synthetic Seed ◽  
Seed Technology

Antioxidant defense and photosynthesis for non-indigenous mangrove species Sonneratia apetala and Laguncularia racemosa under NaCl stress

2013 ◽  
Vol 37 (5) ◽  
pp. 443-453 ◽  
Author(s):  
Jian CHEN ◽  
Ni-Ya LI ◽  
Qiang LIU ◽  
Cai-Rong ZHONG ◽  
Min HUANG ◽  
...  
Keyword(s):  
Antioxidant Defense ◽  
Nacl Stress ◽  
Laguncularia Racemosa ◽  
Mangrove Species ◽  
Sonneratia Apetala
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