Silicon in the Embriogenic Potential of Callus in vitro of Passiflora edulis

Adding silicon to the culture medium may contribute, to improve many growth parameters including embryogenesis and organogenesis. The objective of this study was to evaluate the embryogenic potential of passion fruit in vitro (Passiflora edulis f. Flavicarpa O. Deg), submitted to different concentrations of silicon. Nodal segments of plants were inoculated at five concentrations of silicic acid added to the culture medium, 0.0; 0.5; 1.0; 1.5 and 2.0 g L-1. After 60 days, the visual characteristics (% contamination, callus, bud, root and oxidation), cytochemical test and microanalysis of X-rays evaluated. Concentrations of 0.5; 1.0 and 2.0 g L-1 of silicon did not differ in relation to callus formation. By the cytochemical test, all treatments presented embryogenic cells indicated by the reaction to acetic Carmine and, little reaction to the Evans blue associated with cell death. By the microanalysis of X-rays, the nutrients sulfur and phosphorus, presented significant absorption responses at the concentration of 1.5 g L-1 of silicon in comparison to the other concentrations. The silicon added to the culture medium has a response in the formation of cells with embryogenic potential in nodal segments of passion fruit. The concentration of 2.0 g L-1 of silicon acid (H4SiO4) has a high embryogenic potential in passionflower cells, Passiflora edulis f. Flavicarpa O. Deg.

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Induction, Growth Kinetics and Morpho-histological Characterization of Neem Callus

Journal of Agricultural Science ◽

10.5539/jas.v10n6p283 ◽

2018 ◽

Vol 10 (6) ◽

pp. 283

Author(s):

Leila Albuquerque Resende de Oliveira ◽

Annie Carolina Araújo de Oliveira ◽

Caroline De Araújo Machado ◽

Milena Nascimento Cardoso ◽

Fernanda Vieira Santana ◽

...

Keyword(s):

Cell Division ◽

Azadirachta Indica ◽

Culture Medium ◽

Callus Formation ◽

Nodal Segments ◽

Insecticidal Action ◽

Histological Characteristics ◽

Light Brown Color

Azadirachta indica A. Juss, popularly known as neem, is a species native to India, belonging to family Meliaceae, considered the most important plant species with insecticidal action. The aim of this study was to evaluate the influence of growth regulators on induction and growth of neem callus and to observe their viability for embryogenesis through morpho-histological characteristics. In vitro germinated plants were used for excision of nodal explants. These segments were inoculated in Murashige and Skoog culture medium containing 1.0 mg/l 2,4-D (2,4-dichlorophenoxyacetic) combined with BAP (6-benzylaminopurine) at the following concentrations: 0.0, 0.5, 1.0 and 2.0 mg/l (T1, T2, T3 and T4 respectively), for callus induction. At 0 (mass of nodal segments without callus), 20, 40 and 60 days of culture, the percentage of callus formation was observed and the callus weight was measured for each treatment and at the end of the 60 days, consistency, color, and cell histology were evaluated. There was callus formation in all treatments tested. The highest induction of Azadirachta indica A. Juss callus is observed in the presence of 1.0 mg/l 2,4-D + 2.0 mg/l BAP, with callus showing light brown color, friable consistency and rounded cells with intense cell division, typical of cells with potential embryogenic capacity.

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Rhizogenesis in in vitro shoot cultures of passion fruit (Passiflora edulis f. flavicarpa Deg.) is affected by ethylene precursor and by inhibitors

International Journal of Horticultural Science ◽

10.31421/ijhs/7/1/247 ◽

2001 ◽

Vol 7 (1) ◽

Author(s):

W. M. Marota ◽

W. C. Otoni ◽

M. Carnelossi ◽

E. Silva ◽

A. A. Azevedo ◽

...

Keyword(s):

Ethylene Production ◽

Time Course ◽

Root Formation ◽

Callus Formation ◽

Passion Fruit ◽

Shoot Cultures ◽

Ethylene Inhibitors ◽

Beneficial Effects ◽

Ethylene Precursor

The effects of the ethylene precursor ACC and two inhibitors, AgNO3 and AVG, on root formation were tested in in vitro shoots of passion fruit (Passiflora Midis f.flavicalpa Deg.). The organogenic response was assessed on the basis of percentage of shoot-forming. roots, root number and length. The time course of ethylene production was also monitored. ACC inhibited root formation by delaying root emergence and increasine, callus formation at the basis of the shoots. In addition, ACC caused a marked increase in ethylene production, coupled to leaf chlorosis and senescence with lower rooting frequencies, number and length of roots. IAA supplementation increased ethylene production. Both ethylene inhibitors, AgNO3 and AVG, at appropriate concentrations reduced callus formation at the basis of shoots. AVG increased the number of roots per shoot, but drastically reduced length of differentiated roots. Regarding to leaf pigments, ACC promoted a marked reduction on carotenoids and total chlorophyll, whereas AVG and AgNO3 delayed explant senescence and pigments degradation, not differing from IAA supplemented and non-supplemented control treatments. The results confirm previous reports on the beneficial effects of ethylene inhibitors on in vitro rooting and suggest its reliability to be used as an alternative approach to evaluate sensitivity of Passiflora species to ethylene.

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Types and concentrations of cytokinins in the micropropagation of Anthurium maricense

Revista Agro mbiente On-line ◽

10.18227/1982-8470ragro.v12i2.4671 ◽

2018 ◽

Vol 12 (2) ◽

pp. 117

Author(s):

Cecília Moreira Serafim ◽

Arlene Santisteban Campos ◽

Priscila Bezerra Dos Santos Melo ◽

Ana Cecília Ribeiro de Castro ◽

Ana Cristina Portugal Pinto de Carvalho

Keyword(s):

Light Intensity ◽

Culture Medium ◽

Culture Media ◽

In Vitro Regeneration ◽

Nodal Segments ◽

Nodal Segment ◽

Viable Option ◽

Growth Room ◽

In Vitro Induction

Faced with the demand for plants potted for their foliage, Anthurium maricense is seen as a viable option. However, most of the studies on obtaining micropropagated plantlets are for A. andraeanum, with nothing yet reported for A. maricense. The aim of this study therefore, was to evaluate the effect of four cytokinins in different concentrations, on the in vitro induction of shoots from nodal segments of A. maricense. The experimental design was completely randomised in a 4 x 4 factorial scheme, with four cytokinins (BAP, ZEA, CIN and 2iP) and 4 concentrations (0, 2.22, 4.44 and 6.66 μM), for a total of 16 treatments, with 6 replications of five test tubes, and using one nodal segment. Cultures were kept in a growth room at 25 ± 2°C, a photoperiod of 16 h and a light intensity of 30 μmolm-2 s-1 for 60 days. After this period, the number of shoots formed per node was evaluated. The addition of a cytokinin to the culture medium was determinant for the in vitro regeneration of shoots in A. maricense. The greatest estimated number of shoot formations in A. maricense were obtained in the culture media containing ZEA (3.87) and BAP (3.30), both at concentration of 6.66 μM.

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Essential factors for in vitro regeneration of rose and a protocol for plant regeneration from leaves

Horticultural Science ◽

10.17221/12/2017-hortsci ◽

2018 ◽

Vol 45 (No. 2) ◽

pp. 83-91

Author(s):

Anber Mahmoud Ahmed Hassanein ◽

Inas Mohamed Ali Mahmoud

Keyword(s):

Indole Acetic Acid ◽

Culture Medium ◽

Callus Formation ◽

In Vitro Regeneration ◽

Shoot Elongation ◽

Favorable Effect ◽

Indole Butyric Acid ◽

Leaf Discs ◽

Better Than

In vitro propagation of Rosa hybrida, L. cv. ‘Eiffel Tower’ was improved by the addition of thidiazuron (TDZ) and silver nitrate (AgNo3) to the culture medium. The combination of auxin and cytokinins was indispensable for inducing response from leaf discs. Maintaining cultures under dark was better than light for callus formation and quality. The source of explants was vital in the regeneration process wherein situ explants produced callus while, in vitro explants regenerated somatic embryos and shoots. Gibberellic acid (GA3) had a favorable effect where in vitro explants showed somatic embryogenesis with no shoots on media containing TDZ however, 37% of explants regenerated shoots directly on medium containing GA3. The presence of benzyl adenine (BA) was essential for shoot elongation, and indole butyric acid (IBA) was better than indole acetic acid (IAA) for rooting. The optimum conditions produced rooted plants from leaf discs within ten weeks. The reported results clarify factors controlling in vitro regeneration of R. hybrida, and provide a rapid protocol allowing further improvements of rose.

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LIGHT QUALITY IN THE IN VITRO INTRODUCTION OF Corymbia HYBRID CLONES

Revista Árvore ◽

10.1590/1806-90882018000600004 ◽

2018 ◽

Vol 42 (6) ◽

Cited By ~ 1

Author(s):

Denys Matheus Santana Costa Souza ◽

Aloisio Xavier ◽

Wagner Campos Otoni ◽

Natane Amaral Miranda ◽

Joane Helena Maggioni

Keyword(s):

Light Source ◽

Culture Medium ◽

Light Quality ◽

Shoot Length ◽

Nodal Segments ◽

Fluorescent Lamp ◽

Corymbia Citriodora ◽

Hybrid Clones ◽

Effect Of Light

ABSTRACT Micropropagation via axillary bud proliferation is recommended for rejuvenation or reinvigoration of selected clones, as well as for improving clonal seedlings rooting. The success of a micropropagation protocol depends on the in vitro introduction, since following phases, multiplication, elongation, and rooting can only take place once the aseptic crop with vegetative vigor has been established. This study aims to assess the effect of light on the in vitro introduction of hybrid clones of Corymbia torelliana x C. citriodora e Corymbia citriodora x C. torelliana by the micropropagation technique through proliferation by axillary buds. The mini-stumps, suppliers of explants for in vitro introduction, were conducted in semi-hydroclonal mini-clonal hedge. Nodal segments from three Corymbia torelliana x C. citriodora (TC01, TC02 e TC03) clones and one Corymbia citriodora x C. torelliana (CT01) clone were collected, disinfested and inoculated in JADS culture medium, in order to compare the effects of light quality from a dark/fluorescent lamp, a fluorescent lamp, and white and red/blue LEDs. At 30 days after inoculation, the following characteristics were evaluated: average contamination percentage, oxidation, non-reactive explants, shoot length and average number of shoots per explant greater than 0.5 cm. Gathered data showed that the use of red/blue LED light source obtained the best results in all assessed characteristics in the in vitro introduction.

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Biotechnological methods of in vitro propagation in willows (Salix spp.)

Open Life Sciences ◽

10.2478/s11535-012-0069-5 ◽

2012 ◽

Vol 7 (5) ◽

pp. 931-940 ◽

Cited By ~ 3

Author(s):

Dagmar Skálová ◽

Božena Navrátilová ◽

Lenka Richterová ◽

Michal Knit ◽

Michal Sochor ◽

...

Keyword(s):

Central Europe ◽

In Vitro Propagation ◽

Culture Medium ◽

Reproductive Potential ◽

Ovule Culture ◽

Nodal Segments ◽

Young Shoot ◽

Fragmented Populations ◽

Salix Spp

AbstractMany populations of high-mountainous relic dioecious willows in Central Europe only consist of female individuals and are thus limited in their reproductive potential. We completed micropropagation experiments with shoot apexes and nodal segments of common and endangered willow (Salix) species, which can help to reintroduce autochthonous genotypes to their natural sites. Until recently, cultivation of green young shoot apexes of S. alba and S. lapponum showed the highest percentage of regeneration. We successfully applied the two-times-sterilisation due to high contamination of natural explants. The OK medium was the most efficient culture medium. In vitro propagation of willows with unisexual catkins, anther and ovule cultures were tested and optimised. Isolated anthers were cultivated on selected media and then microcallus and calluses of S. caprea and calluses of S. viminalis were formed on the A medium. Among various tested and optimised media for the ovule culture, the CP medium was the most efficient one. In this case, only the microcalluses of S. viminalis were observed. We developed biotechnological procedures that can be useful in conserving fragmented populations of high-mountainous willows.

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Anatomical and ultrastructural analyses of in vitro organogenesis from root explants of commercial passion fruit (Passiflora edulis Sims)

Plant Cell Tissue and Organ Culture (PCTOC) ◽

10.1007/s11240-012-0171-4 ◽

2012 ◽

Vol 111 (1) ◽

pp. 69-78 ◽

Cited By ~ 24

Author(s):

Diego Ismael Rocha ◽

Lorena Melo Vieira ◽

Francisco André Ossamu Tanaka ◽

Luzimar Campos da Silva ◽

Wagner Campos Otoni

Keyword(s):

Passion Fruit ◽

Root Explants ◽

Passiflora Edulis ◽

In Vitro Organogenesis ◽

Passiflora Edulis Sims

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Antioxidant activity of aqueous extract of passion fruit (Passiflora edulis) leaves: In vitro and in vivo study

Food Research International ◽

10.1016/j.foodres.2012.12.043 ◽

2013 ◽

Vol 53 (2) ◽

pp. 882-890 ◽

Cited By ~ 68

Author(s):

Juliana Kelly da Silva ◽

Cinthia Baú Betim Cazarin ◽

Talita C. Colomeu ◽

Ângela Giovana Batista ◽

Laura M.M. Meletti ◽

...

Keyword(s):

Antioxidant Activity ◽

Aqueous Extract ◽

Passion Fruit ◽

In Vivo Study ◽

Passiflora Edulis

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Micropropagation of Isoplexis chalcantha Svent, O´Shanahan from Mature Plants

Plant Tissue Culture and Biotechnology ◽

10.3329/ptcb.v18i2.3395 ◽

1970 ◽

Vol 18 (2) ◽

pp. 131-137 ◽

Cited By ~ 1

Author(s):

S. Mederos-Molina

Keyword(s):

Culture Medium ◽

Culture Media ◽

Nodal Segments ◽

Axillary Shoots ◽

In Vitro Shoots ◽

Half Strength ◽

Modified Ms Medium ◽

High Degree ◽

First Time

Culture medium requirements for micropropagation of Isoplexis chalcantha was achieved for the first time after high degree of contamination and phenolic exudates were detected and solved. Cultures were established from axillary shoots using juvenile branches collected from this medicinal plant. Most satisfying results were obtained using a solidified and a modified MS medium (NO3- : NH4+ ratios) enriched with ascorbic acid or soluble PVP plus GA3, BAP and NAA. Explants (nodal segments) were used for in vitro shoots multiplication and best results were achieved with modified MS plus BAP and auxins. Vigorous shoots rooted without symptoms in the half-strength modified MS enriched with low concentration of IBA. Key words: Isoplexis chalcantha, axillary shoots, contamination, phenolic exudates, culture media, NO3- : NH4+ ratios D.O.I. 10.3329/ptcb.v18i2.3395 Plant Tissue Cult. & Biotech. 18(2): 131-137, 2008 (December)

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